|
5
|
1 <tool id="bam2wig" name="BAM to Wiggle">
|
|
|
2 <description>
|
|
|
3 converts all types of RNA-seq data from .bam to .wig
|
|
|
4 </description>
|
|
|
5 <requirements>
|
|
|
6 <requirement type="package" version="2.3.7">rseqc</requirement>
|
|
|
7 </requirements>
|
|
|
8 <command interpreter="python">
|
|
|
9 samtoolshelper.py bam2wig.py -i $input -s $chromsize -o outfile
|
|
|
10
|
|
|
11 #if str($strand_type.strand_specific) == "pair"
|
|
|
12 -d
|
|
|
13 #if str($strand_type.pair_type) == "sd"
|
|
|
14 '1++,1--,2+-,2-+'
|
|
|
15 #else
|
|
|
16 '1+-,1-+,2++,2--'
|
|
|
17 #end if
|
|
|
18 #end if
|
|
|
19
|
|
|
20 #if str($strand_type.strand_specific) == "single"
|
|
|
21 -d
|
|
|
22 #if str($strand_type.single_type) == "s"
|
|
|
23 '++,--'
|
|
|
24 #else
|
|
|
25 '+-,-+'
|
|
|
26 #end if
|
|
|
27 #end if
|
|
|
28
|
|
|
29 #if $wigsum.wigsum_type
|
|
|
30 -t $wigsum.totalwig
|
|
|
31 #end if
|
|
|
32
|
|
|
33 #if $skipmultihits
|
|
|
34 -u
|
|
|
35 #end if
|
|
|
36 </command>
|
|
|
37 <inputs>
|
|
|
38 <param name="input" type="data" label="Input .bam File" format="bam" />
|
|
|
39 <param name="chromsize" type="data" label="Chromosome size file (tab or space separated)" format="txt,tabular" />
|
|
|
40 <param name="skipmultihits" type="boolean" label="Skip Multiple Hit Reads/Only Use Uniquely Mapped Reads" value="false" />
|
|
|
41 <conditional name="wigsum">
|
|
|
42 <param name="wigsum_type" type="boolean" label="Specify wigsum?" value="false">
|
|
|
43 </param>
|
|
|
44 <when value="true">
|
|
|
45 <param name="totalwig" value="0" type="integer" label="specified wigsum" />
|
|
|
46 </when>
|
|
|
47 <when value="false"></when>
|
|
|
48 </conditional>
|
|
|
49 <conditional name="strand_type">
|
|
|
50 <param name="strand_specific" type="select" label="Strand-specific?" value="none">
|
|
|
51 <option value="none">none</option>
|
|
|
52 <option value="pair">Pair-End RNA-seq</option>
|
|
|
53 <option value="single">Single-End RNA-seq</option>
|
|
|
54 </param>
|
|
|
55 <when value="pair">
|
|
|
56 <param name="pair_type" type="select" display="radio" label="Pair-End Read Type (format: mapped --> parent)" value="sd">
|
|
|
57 <option value="sd"> read1 (positive --> positive; negative --> negative), read2 (positive --> negative; negative --> positive)</option>
|
|
|
58 <option value="ds">read1 (positive --> negative; negative --> positive), read2 (positive --> positive; negative --> negative)</option>
|
|
|
59 </param>
|
|
|
60 </when>
|
|
|
61 <when value="single">
|
|
|
62 <param name="single_type" type="select" display="radio" label="Single-End Read Type (format: mapped --> parent)" value="s">
|
|
|
63 <option value="s">positive --> positive; negative --> negative</option>
|
|
|
64 <option value="d">positive --> negative; negative --> positive</option>
|
|
|
65 </param>
|
|
|
66 </when>
|
|
|
67 <when value="none"></when>
|
|
|
68 </conditional>
|
|
|
69 </inputs>
|
|
|
70 <outputs>
|
|
|
71 <data format="wig" name="output" from_work_dir="outfile.wig">
|
|
|
72 <filter>strand_type['strand_specific'] == 'none'</filter>
|
|
|
73 </data>
|
|
|
74 <data format="wig" name="outputfwd" from_work_dir="outfile_Forward.wig">
|
|
|
75 <filter>strand_type['strand_specific'] != 'none'</filter>
|
|
|
76 </data>
|
|
|
77 <data format="wig" name="outputrv" from_work_dir="outfile_Reverse.wig">
|
|
|
78 <filter>strand_type['strand_specific'] != 'none'</filter>
|
|
|
79 </data>
|
|
|
80 </outputs>
|
|
|
81 <tests>
|
|
|
82 <test>
|
|
|
83 <param name="input" value="Pairend_nonStrandSpecific_36mer_Human_hg19.bam" />
|
|
|
84 <param name="chromsize" value="sample.hg19.chrom.sizes.txt" />
|
|
|
85 <param name="skipmultihits" value="false" />
|
|
|
86 <param name="wigsum.wigsum_type" value="false" />
|
|
|
87 <param name="strand_type.strand_specific" value="none" />
|
|
|
88 <output name="output" file="outfile.wig" />
|
|
|
89 </test>
|
|
|
90 </tests>
|
|
|
91 <help>
|
|
|
92 .. image:: https://code.google.com/p/rseqc/logo?cct=1336721062
|
|
|
93
|
|
|
94 -----
|
|
|
95
|
|
|
96 About RSeQC
|
|
|
97 +++++++++++
|
|
|
98
|
|
|
99 The RSeQC package provides a number of useful modules that can comprehensively evaluate high throughput sequence data especially RNA-seq data. “Basic modules” quickly inspect sequence quality, nucleotide composition bias, PCR bias and GC bias, while “RNA-seq specific modules” investigate sequencing saturation status of both splicing junction detection and expression estimation, mapped reads clipping profile, mapped reads distribution, coverage uniformity over gene body, reproducibility, strand specificity and splice junction annotation.
|
|
|
100
|
|
|
101 The RSeQC package is licensed under the GNU GPL v3 license.
|
|
|
102
|
|
|
103 Inputs
|
|
|
104 ++++++++++++++
|
|
|
105
|
|
|
106 Input BAM file
|
|
|
107 Alignment file in BAM format (SAM is not supported). BAM file will be sorted and indexed using samTools.
|
|
|
108
|
|
|
109 Chromosome size file
|
|
|
110 Tab or space separated text file with 2 columns: first column is chromosome name, second column is size of the chromosome. Chromosome names (such as "chr1") should be consistent between this file and BAM file.
|
|
|
111
|
|
|
112 Specified wigsum (default=none)
|
|
|
113 Specified wigsum. Wigsum of 100000000 equals to coverage achieved by 1 million 100nt reads. Ignore this option to disable normalization.
|
|
|
114
|
|
|
115 Skip multiple Hit reads
|
|
|
116 skips multiple hit reads or only use uniquely mapped reads
|
|
|
117
|
|
|
118 Strand-specific (default=none)
|
|
|
119 How read(s) were stranded during sequencing. If you are not sure about the strand rule, run infer_experiment.py
|
|
|
120
|
|
|
121 Outputs
|
|
|
122 ++++++++++++++
|
|
|
123
|
|
|
124 If RNA-seq is not strand specific, one wig file will be generated, if RNA-seq
|
|
|
125 is strand specific, two wig files corresponding to Forward and Reverse will be generated.
|
|
|
126
|
|
|
127
|
|
|
128 </help>
|
|
|
129 </tool> |
