Mercurial > repos > nilesh > rseqc
diff read_GC.xml @ 3:71ed55a3515a draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rseqc commit 37fb1988971807c6a072e1afd98eeea02329ee83
author | iuc |
---|---|
date | Tue, 14 Mar 2017 10:22:57 -0400 |
parents | f92b87abef3d |
children | d7f6b3653d84 |
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--- a/read_GC.xml Thu Jul 18 11:01:08 2013 -0500 +++ b/read_GC.xml Tue Mar 14 10:22:57 2017 -0400 @@ -1,53 +1,74 @@ -<tool id="read_GC" name="Read GC"> - <description>determines GC% and read count</description> - <requirements> - <requirement type="package" version="2.15.1">R</requirement> - <requirement type="package" version="2.3.7">rseqc</requirement> - </requirements> - <command interpreter="python"> read_GC.py -i $input -o output - </command> - <inputs> - <param name="input" type="data" format="bam,sam" label="input bam/sam file" /> - </inputs> - <outputs> - <data format="xls" name="outputxls" from_work_dir="output.GC.xls"/> - <data format="r" name="outputr" from_work_dir="output.GC_plot.r" /> - <data format="pdf" name="outputpdf" from_work_dir="output.GC_plot.pdf" /> - </outputs> - <tests> - <test> - <param name="input" value="Pairend_nonStrandSpecific_36mer_Human_hg19.bam" /> - <output name-"outputxls" file="Pairend_nonStrandSpecific_36mer_Human_hg19.bam" /> - <output name="outputr" file="readgcout.GC_plot.r" /> - <output name="outputpdf" file="readgcout.GC_plot.pdf" /> - </test> - </tests> - <help> - .. image:: https://code.google.com/p/rseqc/logo?cct=1336721062 +<tool id="rseqc_read_GC" name="Read GC" version="@WRAPPER_VERSION@"> + <description>determines GC% and read count</description> + + <macros> + <import>rseqc_macros.xml</import> + </macros> + + <expand macro="requirements" /> + + <expand macro="stdio" /> + + <version_command><![CDATA[read_GC.py --version]]></version_command> + + <command><![CDATA[ + read_GC.py + --input-file '${input}' + --out-prefix output + --mapq ${mapq} + ]]> + </command> ------ + <inputs> + <expand macro="bam_sam_param" /> + <expand macro="mapq_param" /> + <expand macro="rscript_output_param" /> + </inputs> + + <outputs> + <expand macro="pdf_output_data" filename="output.GC_plot.pdf" /> + <expand macro="xls_output_data" filename="output.GC.xls" /> + <expand macro="rscript_output_data" filename="output.GC_plot.r" /> + </outputs> -About RSeQC -+++++++++++ + <tests> + <test> + <param name="input" value="pairend_strandspecific_51mer_hg19_chr1_1-100000.bam" /> + <param name="rscript_output" value="true" /> + <output name="outputxls" file="output.GC.xls" /> + <output name="outputr" file="output.GC_plot.r" /> + <output name="outputpdf" file="output.GC_plot.pdf" compare="sim_size" /> + </test> + </tests> -The RSeQC package provides a number of useful modules that can comprehensively evaluate high throughput sequence data especially RNA-seq data. “Basic modules” quickly inspect sequence quality, nucleotide composition bias, PCR bias and GC bias, while “RNA-seq specific modules” investigate sequencing saturation status of both splicing junction detection and expression estimation, mapped reads clipping profile, mapped reads distribution, coverage uniformity over gene body, reproducibility, strand specificity and splice junction annotation. + <help><![CDATA[ +read_GC.py +++++++++++ -The RSeQC package is licensed under the GNU GPL v3 license. Inputs ++++++++++++++ Input BAM/SAM file - Alignment file in BAM/SAM format. + Alignment file in BAM/SAM format. Output ++++++++++++++ 1. output.GC.xls: Two column, plain text file, first column is GC%, second column is read count 2. output.GC_plot.r: R script to generate pdf file. -3. output.GC_plot.pdf: graphical output generated from R script. +3. output.GC_plot.pdf: graphical output generated from R script. + +.. image:: $PATH_TO_IMAGES/read_gc.png + :height: 600 px + :width: 600 px + :scale: 80 % -.. image:: http://dldcc-web.brc.bcm.edu/lilab/liguow/RSeQC/figure/read_gc.png +@ABOUT@ - </help> +]]> + </help> + + <expand macro="citations" /> + </tool>