Mercurial > repos > mvdbeek > r_goseq_1_22_0
diff goseq.xml @ 0:a6427f7893b0 draft
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/goseq_1_22_0 commit fdd0811efc61c31f88ff17096fbe8ee8cfacd766-dirty
author | mvdbeek |
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date | Fri, 26 Feb 2016 06:57:47 -0500 |
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children | ba71bc50299b |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/goseq.xml Fri Feb 26 06:57:47 2016 -0500 @@ -0,0 +1,80 @@ +<tool id="goseq" name="goseq gene ontology analyser" version="0.1.2"> + <description /> + <requirements> + <requirement type="package" version="3.2.1">R</requirement> + <requirement type="package" version="1.22.0">goseq</requirement> + </requirements> + <command interpreter="Rscript"> + goseq.r --dge_file "$dge_file" + --p_adj_column "$p_adj_column" + --cutoff "$p_adj_cutoff" + #if $source.use_length_file == "yes": + --length_file "$length_file" + #end if + --genome "$genome" + --gene_id "$gene_id" + --wallenius_tab "$wallenius_tab" + --sampling_tab "$sampling_tab" + --nobias_tab "$nobias_tab" + --length_bias_plot "$length_bias_plot" + --sample_vs_wallenius_plot "$sample_vs_wallenius_plot" + --repcnt "$repcnt" + </command> + <inputs> + <param help="deseq2/edger/limma differential gene expression list" label="DGE list" name="dge_file" type="data" format="tabular" /> + <param help="Select the column that contains the multiple-testing corrected p-value" label="p adjust column" name="p_adj_column" type="data_column" data_ref="dge_file"/> + <param help="Typically 0.05 after multiple testing correction" max="1" label="Minimum p adjust value to consider genes as differentially expressed" name="p_adj_cutoff" type="float" value="0.05" /> + <conditional name="source"> + <param help="This is needed if the gene length is not available in goseq. e.g. hg38 and mm10." label="Use gene length file?" name="use_length_file" type="select"> + <option value="no">no</option> + <option value="yes">yes</option> + </param> + <when value="yes"> + <param label="Gene length file" name="length_file" type="data" format="tabular" required="false" /> + </when> + </conditional> + <param help="Needed to retrieve gene length for length correction" label="Select the genome source" name="genome" size="3" type="select"> + <options from_table="go_genomes"></options> + </param> + <param help="Needed for GO analysis" label="Select gene identifier" name="gene_id" type="select"> + <options from_table="go_gene_ids"></options> + </param> + <param help="Do this many random samplings. Set to 0 to not do sampling. Larger values take a long time" label="Number of random sampling" name="repcnt" size="3" type="integer" min="0" max="10000" value="0" /> + </inputs> + <outputs> + <data format="pdf" label="length bias plot" name="length_bias_plot" /> + <data format="pdf" label="Plot P-value from sampling against wallenius distribution" name="sample_vs_wallenius_plot"> + <filter>repcnt == "0"</filter> + </data> + <data format="tabular" label="Ranked category list - wallenius" name="wallenius_tab" /> + <data format="tabular" label="Ranked category list - sampling" name="sampling_tab"> + <filter>repcnt == "0"</filter> + </data> + <data format="tabular" label="Ranked category list - no length bias correction" name="nobias_tab" /> + </outputs> + <tests> + <test> + <param name="dge_file" value="dge_list.tab" /> + <param name="use_length_file" value="no" /> + <param name="p_adj_column" value="2" /> + <param name="genome" value="hg19" /> + <param name="gene_id" value="ensGene" /> + <output name="wallenius_tab" file="wall.tab" /> + </test> + </tests> + <help> + + **What it does** + + Detects Gene Ontology and/or other user defined categories which are over/under represented in RNA-seq data. + + Options map closely to the excellent manual_ + + .. _manual: https://bioconductor.org/packages/release/bioc/vignettes/goseq/inst/doc/goseq.pdf + + + </help> + <citations> + <citation type="doi">10.1186/gb-2010-11-2-r14</citation> + </citations> +</tool>