# HG changeset patch
# User mvdbeek
# Date 1498052289 14400
# Node ID 5b08a7a4caa9935a560176889a0ccad7ebd468ec
# Parent  a01f2a172eeb89366260fe497e21f76e02b275ce
planemo upload for repository https://github.com/bardin-lab/readtagger/tree/master/galaxy commit 6e01a2e472ebbb07ce5181b836bae8bc5c7ecf36

diff -r a01f2a172eeb -r 5b08a7a4caa9 add_matesequence.xml
--- a/add_matesequence.xml	Wed Jun 21 07:24:36 2017 -0400
+++ b/add_matesequence.xml	Wed Jun 21 09:38:09 2017 -0400
@@ -1,7 +1,7 @@
-<tool id="add_matesequence" name="Add matesequence" version="0.3.24">
+<tool id="add_matesequence" name="Add matesequence" version="0.3.25">
     <description>into tag field</description>
     <requirements>
-        <requirement type="package" version="0.3.24">readtagger</requirement>
+        <requirement type="package" version="0.3.25">readtagger</requirement>
     </requirements>
     <version_command>add_matesequence --version</version_command>
     <command detect_errors="aggressive"><![CDATA[
diff -r a01f2a172eeb -r 5b08a7a4caa9 allow_dovetailing.xml
--- a/allow_dovetailing.xml	Wed Jun 21 07:24:36 2017 -0400
+++ b/allow_dovetailing.xml	Wed Jun 21 09:38:09 2017 -0400
@@ -1,7 +1,7 @@
-<tool id="allow_dovetailing" name="Allow dovetailing" version="0.3.24">
+<tool id="allow_dovetailing" name="Allow dovetailing" version="0.3.25">
     <description>modifies proper_pair flag in bam files</description>
     <requirements>
-        <requirement type="package" version="0.3.24">readtagger</requirement>
+        <requirement type="package" version="0.3.25">readtagger</requirement>
     </requirements>
     <command detect_errors="aggressive"><![CDATA[
         allow_dovetailing -i '$input' -o '$output'
diff -r a01f2a172eeb -r 5b08a7a4caa9 bam_readtagger.xml
--- a/bam_readtagger.xml	Wed Jun 21 07:24:36 2017 -0400
+++ b/bam_readtagger.xml	Wed Jun 21 09:38:09 2017 -0400
@@ -1,10 +1,10 @@
-<tool id="bam_readtagger" name="Tag alignment files" version="0.3.24">
+<tool id="bam_readtagger" name="Tag alignment files" version="0.3.25">
     <description>from multiple bam files</description>
     <macros>
         <import>macros.xml</import>
     </macros>
     <requirements>
-        <requirement type="package" version="0.3.24">readtagger</requirement>
+        <requirement type="package" version="0.3.25">readtagger</requirement>
     </requirements>
     <command detect_errors="aggressive"><![CDATA[
         readtagger -t '$tag_file' -s
diff -r a01f2a172eeb -r 5b08a7a4caa9 deploy.sh
diff -r a01f2a172eeb -r 5b08a7a4caa9 findcluster.xml
--- a/findcluster.xml	Wed Jun 21 07:24:36 2017 -0400
+++ b/findcluster.xml	Wed Jun 21 09:38:09 2017 -0400
@@ -1,7 +1,10 @@
-<tool id="findcluster" name="Find clusters of reads" version="0.3.24">
+<tool id="findcluster" name="Find clusters of reads" version="0.3.25">
     <description>in bam files</description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
     <requirements>
-        <requirement type="package" version="0.3.24">readtagger</requirement>
+        <requirement type="package" version="0.3.25">readtagger</requirement>
     </requirements>
     <version_command>findcluster --version</version_command>
     <command detect_errors="aggressive"><![CDATA[
@@ -9,17 +12,19 @@
         ln -f -s $input.metadata.bam_index input.bam.bai &&
         findcluster
         --input_path input.bam
-       #if $transposon_reference_fasta:
-            --transposon_reference_fasta '$transposon_reference_fasta'
-        #end if
-        #if $genome_reference_fasta:
-            --genome_reference_fasta '$genome_reference_fasta'
+        #if $transposon_source.ref_file:
+            #if str($transposon_source.reference_source_selector) == "history":
+                --transposon_reference_fasta '$transposon_source.ref_file'
+            #else :
+                --transposon_bwa_index '$reference_source.ref_file.fields.path'
+            #end if
         #end if
-       #if $transposon_bwa_index:
-            --transposon_bwa_index '$transposon_bwa_index'
-        #end if
-        #if $genome_bwa_index:
-            --genome_bwa_index '$genome_bwa_index'
+        #if $genome_source.ref_file:
+            #if str($genome_source.reference_source_selector) == "history":
+                --genome_reference_fasta '$genome_source.ref_file'
+            #else :
+                --genome_bwa_index '$reference_source.ref_file.fields.path'
+            #end if
         #end if
         --output_bam '$output_bam'
         --output_gff '$output_gff'
@@ -29,20 +34,9 @@
     ]]></command>
     <inputs>
         <param name="input" argument="--input_path" type="data" format="bam"/>
-        <param argument="--transposon_reference_fasta" label="Transposon Fasta" help="Reconstructed contigs at clusters will be blasted against this sequence." type="data" format="fasta" optional="True"/>
-        <param argument="--genome_reference_fasta" label="Genome Fasta" help="Reconstructed contigs at clusters will be blasted against this sequence." type="data" format="fasta" optional="True"/>
-        <param argument="--transposon_bwa_index" label="Transposon BWA index" help="Reconstructed contigs at clusters will be blasted against this sequence." type="select" optional="True">
-            <options from_data_table="bwa_mem_indexes">
-                <filter type="sort_by" column="2" />
-                <validator type="no_options" message="No indexes are available" />
-            </options>
-        </param>
-        <param argument="--genome_bwa_index" label="Genome BWA index" help="Reconstructed contigs at clusters will be blasted against this sequence." type="select" optional="True">
-            <options from_data_table="bwa_mem_indexes">
-                <filter type="sort_by" column="2" />
-                <validator type="no_options" message="No indexes are available" />
-            </options>
-        </param>
+
+        <expand macro="reference_source_conditional" reference_type="transposon"/>
+        <expand macro="reference_source_conditional" reference_type="genome"/>
     </inputs>
     <outputs>
         <data name="output_bam" format="bam" label="findcluster BAM on $on_string"/>
@@ -57,7 +51,8 @@
         </test>
         <test>
             <param name="input" value="extended_and_annotated_roi.bam" ftype="bam"/>
-            <param name="transposon_reference_fasta" value="reference.fasta" ftype="fasta"/>
+            <param name="transposon_source|reference_source_selector" value="history"/>
+            <param name="transposon_source|ref_file" value="reference.fasta" ftype="fasta"/>
             <output name="output_bam" file="three_cluster_out.bam" ftype="bam" lines_diff="2"/>
             <output name="output_gff">
                 <assert_contents>
@@ -73,24 +68,35 @@
 
       Find clusters of reads that support a TE insertion.
 
-        Options:
+    Options:
       --input_path PATH               Find cluster in this BAM file.
+      --region TEXT                   Find clusters in this Region (Format is
+                                      chrX:2000-1000).
+      --max_proper_pair_size INTEGER  Maximum proper pairs size. If not given will
+                                      be inferred from the data.
       --output_bam PATH               Write out BAM file with cluster information
                                       to this path. Reads will have an additional
                                       "CD" tag to indicate the cluster number
       --output_gff PATH               Write out GFF file with cluster information
                                       to this path.
+      --output_fasta PATH             Write out supporting evidence for clusters
+                                      to this path.
       --sample_name TEXT              Sample name to use when writing out clusters
                                       in GFF file. Default is to infer the name
                                       from the input filename.
       --include_duplicates / --no-include_duplicates
                                       Include reads marked as duplicates when
                                       finding clusters.
-      --transposon_reference_fasta TEXT          Blast cluster contigs against this fasta
-                                      file
-      --blastdb TEXT                  Blast cluster contigs against this blast
-                                      database
+      --transposon_reference_fasta TEXT
+                                      Transposon fasta to align clipped reads to.
+                                      Not necessary if BWA index is provided.
+      --transposon_bwa_index TEXT     Transposon BWA index to align clipped reads
+                                      to
+      --genome_reference_fasta TEXT   Genome fasta to align clipped reads to. Not
+                                      necessary if BWA index is provided.
+      --genome_bwa_index TEXT         Genome BWA index to align clipped reads to
       --threads INTEGER RANGE         Threads to use for cap3 assembly step
+      --shm_dir PATH                  Path to shared memory folder
       --version                       Show the version and exit.
       --help                          Show this message and exit.
 
diff -r a01f2a172eeb -r 5b08a7a4caa9 macros.xml
--- a/macros.xml	Wed Jun 21 07:24:36 2017 -0400
+++ b/macros.xml	Wed Jun 21 09:38:09 2017 -0400
@@ -26,4 +26,24 @@
         <option value="X">X</option>
         <option value="Y">Y</option>
     </xml>
+    <xml name="reference_source_conditional" token_reference_type="reference_type">
+        <conditional name="@REFERENCE_TYPE@_source">
+            <param name="reference_source_selector" type="select" label="Will you select a @REFERENCE_TYPE@ reference genome from your history or use a built-in index?" help="Built-ins were indexed using default options. See `Indexes` section of help below">
+                <option value="cached">Use a built-in @REFERENCE_TYPE@ genome index</option>
+                <option value="history">Use a genome from history and build index</option>
+            </param>
+            <when value="cached">
+                <param name="ref_file" type="select" label="Using @REFERENCE_TYPE@ reference genome" help="Select @REFERENCE_TYPE@ genome from the list" optional="True">
+                    <options from_data_table="bwa_mem_indexes">
+                        <filter type="sort_by" column="2" />
+                        <validator type="no_options" message="No indexes are available" />
+                    </options>
+                    <validator type="no_options" message="A built-in reference genome is not available for the build associated with the selected input file"/>
+                </param>
+            </when>
+            <when value="history">
+                <param name="ref_file" type="data" format="fasta" label="Use the following dataset as the reference sequence" help="You can upload a FASTA sequence to the history and use it as reference" optional="True"/>
+            </when>
+        </conditional>
+    </xml>
 </macros>
diff -r a01f2a172eeb -r 5b08a7a4caa9 update_mapq.xml
--- a/update_mapq.xml	Wed Jun 21 07:24:36 2017 -0400
+++ b/update_mapq.xml	Wed Jun 21 09:38:09 2017 -0400
@@ -1,7 +1,7 @@
-<tool id="update_mapq" name="Update MAPQ score" version="0.3.24">
+<tool id="update_mapq" name="Update MAPQ score" version="0.3.25">
     <description>of supplementary alignments</description>
     <requirements>
-        <requirement type="package" version="0.3.24">readtagger</requirement>
+        <requirement type="package" version="0.3.25">readtagger</requirement>
     </requirements>
     <version_command>update_mapq --version</version_command>
     <command detect_errors="aggressive"><![CDATA[
diff -r a01f2a172eeb -r 5b08a7a4caa9 write_supplementary_fastq.xml
--- a/write_supplementary_fastq.xml	Wed Jun 21 07:24:36 2017 -0400
+++ b/write_supplementary_fastq.xml	Wed Jun 21 09:38:09 2017 -0400
@@ -1,7 +1,7 @@
-<tool id="write_supplementary_fastq" name="Extract supplementary alignments" version="0.3.24">
+<tool id="write_supplementary_fastq" name="Extract supplementary alignments" version="0.3.25">
     <description>from SAM/BAM alignment files as FASTQ</description>
     <requirements>
-        <requirement type="package" version="0.3.24">readtagger</requirement>
+        <requirement type="package" version="0.3.25">readtagger</requirement>
     </requirements>
     <version_command>write_supplementary_fastq --version</version_command>
     <command detect_errors="aggressive"><![CDATA[