annotate stacks_populations.xml @ 7:0b55dd3202c2 draft default tip

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 82e7263f3e57a29362d981e36b82b82063f12ac3
author matthias
date Wed, 26 Jun 2019 05:16:31 -0400
parents 1e260cccfafb
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1 <tool id="stacks2_populations" name="Stacks2: populations" profile="@PROFILE@" version="@STACKS_VERSION@+galaxy@WRAPPER_VERSION@">
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2 <description>Calculate population-level summary statistics</description>
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3 <macros>
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4 <import>macros.xml</import>
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5 </macros>
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6 <expand macro="requirements"/>
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7 <expand macro="version_cmd"/>
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8 <command detect_errors="aggressive"><![CDATA[
0
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9 #import re
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11 mkdir stacks_inputs stacks_outputs
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12
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13 &&
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15 #if str($options_usage.input_type) == 'stacks'
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16 #for $input_file in $options_usage.input_aln
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17 #set $filename = str($input_file.element_identifier)
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18 #if $filename == "catalog.calls" or $filename == "catalog.fa.gz":
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19 ln -s '${input_file}' 'stacks_inputs/${filename}' &&
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20 #elif $filename == "catalog.calls.vcf":
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21 gzip -c '${input_file}' > stacks_inputs/catalog.calls &&
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22 #end if
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23 #end for
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24 #else if str($options_usage.input_type) == 'vcf'
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25 ln -s '$options_usage.input_vcf' 'stacks_inputs/input.vcf' &&
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26 #end if
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27
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28 populations
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30 -t \${GALAXY_SLOTS:-1}
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31
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32 #if str($options_usage.input_type) == 'vcf'
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33 -V stacks_inputs/input.vcf
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34 #else
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35 -P stacks_inputs
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36 #end if
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37 -O stacks_outputs
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38
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39 #if str($popmap) != 'None':
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40 -M '$popmap'
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41 #end if
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42 #if str($batch_size) != '':
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43 -batch_size '$batch_size'
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44 #end if
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45
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46 ## Data filtering
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47
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48 -p $options_filtering.minpop
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49 -r $options_filtering.minperc
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50 -R $options_filtering.min_samples_overall
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51 $options_filtering.filter_haplotype_wise
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52 --min_maf $options_filtering.min_maf
0
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53 --min_mac $options_filtering.min_mac
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54 #if str($options_filtering.max_obs_het)
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55 --max_obs_het $options_filtering.max_obs_het
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56 #end if
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57 ## deprecated https://groups.google.com/forum/#!msg/stacks-users/jdC7gw_MuK4/1v0FG3u7AwAJ
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58 ## #if str($options_filtering.lnl)
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59 ## --lnl_lim $options_filtering.lnl
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60 ## #end if
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61 $options_filtering.filter_single_snp
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62 #if $options_filtering.blacklist
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63 -B '$options_filtering.blacklist'
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64 #end if
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65 #if $options_filtering.whitelist
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66 -W '$options_filtering.whitelist'
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67 #end if
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68
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69 ## Merging and Phasing:
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70 #if str($merge_phase.enzyme) != '':
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71 -e $merge_phase.enzyme
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72 #end if
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73 $merge_phase.merge_sites
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74 --merge_prune_lim $merge_phase.merge_prune_lim
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75
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76 ## Locus stats:
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77 $locus_stats.hwe
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78
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79 ## Fstats
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80 #if str($fstats_conditional.fstats) != 'no':
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81 --fstats
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82 #if str( $fstats_conditional.correction_select.fst_correction ) != "no_corr"
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83 --fst_correction $fstats_conditional.correction_select.fst_correction
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84 --p_value_cutoff $fstats_conditional.correction_select.p_value_cutoff
0
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85 #end if
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86 #end if
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87
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88 ## Kernel-smoothing algorithm:
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89 $kernel_smoothing.options_kernel.kernel
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90 #if str($kernel_smoothing.options_kernel.kernel)!='':
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91 --sigma $kernel_smoothing.options_kernel.sigma
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92 #end if
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93 #if $kernel_smoothing.bootstrap_resampling_mode.bootstrap
0
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94 --bootstrap
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95 #else
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96 $kernel_smoothing.bootstrap_resampling_mode.bootstrap_pifis
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97 $kernel_smoothing.bootstrap_resampling_mode.bootstrap_fst
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98 $kernel_smoothing.bootstrap_resampling_mode.bootstrap_div
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99 $kernel_smoothing.bootstrap_resampling_mode.bootstrap_phist
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100 #end if
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101
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102 #if $kernel_smoothing.bootstrap_resampling_mode.bootstrap or $kernel_smoothing.bootstrap_resampling_mode.bootstrap_pifis or $kernel_smoothing.bootstrap_resampling_mode.bootstrap_fst or $kernel_smoothing.bootstrap_resampling_mode.bootstrap_div or $kernel_smoothing.bootstrap_resampling_mode.bootstrap_phist:
0
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103 #if str($kernel_smoothing.bootstrap_reps)
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104 --bootstrap_reps $kernel_smoothing.bootstrap_reps
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105 #end if
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106 #if $kernel_smoothing.bootstrap_wl
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107 --bootstrap_wl '$kernel_smoothing.bootstrap_wl'
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108 #end if
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109 #end if
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110
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111 ## File output options:
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112
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113 $populations_output.ordered_export
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114 $populations_output.fasta_loci
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115 $populations_output.fasta_samples
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116 $populations_output.vcf
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117 $populations_output.genepop
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118 $populations_output.structure
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119 $populations_output.radpainter
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120 ##$populations_output.phase
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121 ##$populations_output.fastphase
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122 $populations_output.plink
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123 $populations_output.hzar
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124 $populations_output.phylip
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125 $populations_output.phylip_var
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126 ##$populations_output.phylip_var_all
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127 $populations_output.treemix
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128 $populations_output.no_hap_exports
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129 $populations_output.fasta_samples_raw
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130
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131 ## Additional options:
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132
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133 $advanced_options.log_fst_comp
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134
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135 ## populations outputs log info to stdout for vcf input
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136 ## for gstacks input it creates stacks_output/population.log
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137 ## in the latter case we take the log file
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138 ## also for vcf input the output files are named input.p....
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139 ## instead of populations...
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140
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141 #if str($options_usage.input_type) == 'vcf'
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142 #if $output_log
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143 > $output_log
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144 #end if
0
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145 && find stacks_outputs -type f -iname "input.p*" | while read file; do mv "\$file" "\$(echo \$file | sed 's/\/input.p/\/populations/;')"; done
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146 #else
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147 #if $output_log
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148 && mv stacks_outputs/populations.log $output_log
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149 #end if
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150 #end if
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151 ]]></command>
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152
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153 <inputs>
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154 <conditional name="options_usage">
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155 <param name="input_type" type="select" label="Input type" help="select input file type" >
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156 <option value="stacks">Stacks output</option>
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157 <option value="vcf">VCF file</option>
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158 </param>
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159 <when value="stacks">
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160 <expand macro="input_aln_macro"/>
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161 </when>
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162 <when value="vcf">
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163 <param name="input_vcf" format="vcf" type="data" label="VCF file" argument="-V" />
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164 </when>
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165 </conditional>
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166 <param name="popmap" type="data" format="tabular,txt" label="Specify a population map" argument="-M" help="Format is 'SAMPLE1 \t POP1 \n SAMPLE2 ...'." optional="true"/>
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167 <param argument="--batch_size" type="integer" value="" optional="true" label="Number of loci to process in a batch" help="(default: 10,000 in de novo mode; in reference mode, one chromosome per batch). Increase to speed analysis, uses more memory, decrease to save memory)"/>
0
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168
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169 <!-- Data Filtering -->
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170 <section name="options_filtering" title="Data filtering options" expanded="true">
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171
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172 <param name="minperc" argument="-r" type="float" value="0.0" min="0" max="1" label="Minimum percentage of individuals in a population required to process a locus for that population" />
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173 <param name="minpop" argument="-p" type="integer" value="1" label="Minimum number of populations a locus must be present in to process a locus" />
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174 <param name="min_samples_overall" argument="--min-samples-overall" type="float" value="0.0" min="0" max="1" label="minimum percentage of individuals across populations required to process a locus" />
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175 <param name="filter_haplotype_wise" argument="--filter-haplotype-wise" type="boolean" truevalue="--filter-haplotype-wise" falsevalue="" checked="false" label="apply the above filters haplotype wise" help="unshared SNPs will be pruned to reduce haplotype-wise missing data." />
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176 <param argument="--min_maf" type="float" value="0" min="0" max="0.5" label="Minimum minor allele frequency" help="specify a minimum minor allele frequency required to process a nucleotide site at a locus" />
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177 <param argument="--min_mac" type="integer" value="0" min="0" label="Minimum minor allele count" help="specify a minimum minor allele count required to process a nucleotide site at a locus" />
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178 <param argument="--max_obs_het" type="float" value="" min="0" max="1" optional="true" label="Maximum observed heterozygosity required to process a nucleotide site at a locus." />
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179 <!-- deprecated https://groups.google.com/forum/#!msg/stacks-users/jdC7gw_MuK4/1v0FG3u7AwAJ
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180 <param type="float" value="" optional="true" argument="\-\-lnl_lim" label="Filter loci with log likelihood values below this threshold" />-->
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181 <param name="filter_single_snp" type="select" label="Analyse all SNPs per locus" >
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182 <option value="">yes</option>
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183 <option value="--write_single_snp">No: Analyse only the first SNP (--write_single_snp)</option>
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184 <option value="--write_random_snp">No: Analyse only one random SNP (--write_random_snp)</option>
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185 </param>
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186 <param name="whitelist" argument="-W" format="txt,tabular" type="data" optional="true" label="Specify a file containing markers to include in the export" />
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187 <param name="blacklist" argument="-B" format="txt,tabular" type="data" optional="true" label="Specify a file containing markers to exclude from the export" />
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188 </section>
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189
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190 <!-- merging and Phasing -->
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191 <section name="merge_phase" title="Merging and Phasing" expanded="true">
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192 <param name="enzyme" argument="-e" type="select" label="Provide the restriction enzyme used" help="required if generating genomic output" >
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193 <expand macro="enzymes"/>
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194 </param>
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195 <param argument="--merge_sites" truevalue="--merge_sites" falsevalue="" type="boolean" checked="false" label="Merge loci that were produced from the same restriction enzyme cutsite" help="(requires reference-aligned data)" />
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196 <param argument="--merge_prune_lim" type="float" value="1.0" min="0.0" max="1.0" label="Fraction of samples possessing both loci to prune remaining samples from analysis" help="for merging adjacent loci"/>
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197 </section>
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198
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199 <!-- Locus stats -->
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200 <section name="locus_stats" title="Locus Stats" expanded="true">
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201 <param argument="--hwe" truevalue="--hwe" falsevalue="" type="boolean" checked="false" label="Calculate divergence from Hardy-Weinberg equilibrium for each locus" />
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202 </section>
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203
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204 <!-- Fstats -->
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205 <conditional name="fstats_conditional">
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206 <param argument="--fstats" type="select" label="Enable SNP and haplotype-based F statistics" >
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207 <option value="no">No</option>
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208 <option value="yes">Yes</option>
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209 </param>
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210 <when value="no"/>
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211 <when value="yes">
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212 <conditional name="correction_select">
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213 <param argument="--fst_correction" type="select" label="Correction type" help="specify a correction to be applied to Fst values" >
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214 <option value="no_corr">No correction</option>
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215 <option value="p_value">p_value</option>
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216 <option value="bonferroni_win">bonferroni_win</option>
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217 <option value="bonferroni_gen">bonferroni_gen</option>
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218 </param>
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219 <when value="no_corr"/>
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220 <when value="p_value">
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221 <param argument="--p_value_cutoff" type="float" value="0.05" label="P-value cutoff" help="required p-value to keep an Fst measurement (0.05 by default). Also used as base for Bonferroni correction" />
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222 </when>
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223 <when value="bonferroni_win">
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224 <param argument="--p_value_cutoff" type="float" value="0.05" label="P-value cutoff" help="required p-value to keep an Fst measurement (0.05 by default). Also used as base for Bonferroni correction" />
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225 </when>
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226 <when value="bonferroni_gen">
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227 <param argument="--p_value_cutoff" type="float" value="0.05" label="P-value cutoff" help="required p-value to keep an Fst measurement (0.05 by default). Also used as base for Bonferroni correction" />
0
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228 </when>
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229 </conditional>
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230 </when>
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231 </conditional>
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232
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233 <!-- File output options -->
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234 <section name="populations_output" title="Output options" expanded="true">
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235 <param argument="--ordered_export" truevalue="--ordered_export" falsevalue="" type="boolean" checked="false" label="If data is reference aligned, exports will be ordered; only a single representative of each overlapping site." />
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236 <param argument="--fasta_loci" truevalue="--fasta_loci" falsevalue="" type="boolean" checked="false" label="Output locus consensus sequences in FASTA format" />
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237 <param argument="--fasta_samples" truevalue="--fasta_samples" falsevalue="" type="boolean" checked="false" label="Output the sequences of the two haplotypes of each (diploid) sample, for each locus, in FASTA format" />
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238 <param argument="--vcf" truevalue="--vcf" falsevalue="" type="boolean" checked="false" label="Output results in Variant Call Format (VCF)" />
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239 <param argument="--genepop" truevalue="--genepop" falsevalue="" type="boolean" checked="false" label="Output results in GenePop Format" />
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240 <param argument="--structure" truevalue="--structure" falsevalue="" type="boolean" checked="false" label="Output results in Structure Format" />
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241 <param argument="--radpainter" truevalue="--radpainter" falsevalue="" type="boolean" checked="false" label="Output results in fineRADstructure/RADpainter format" />
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242 <!--<param argument="\-\-phase" truevalue="\-\-phase" falsevalue="" type="boolean" checked="false" label="Output genotypes in PHASE format" />-->
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243 <param argument="--fastphase" truevalue="--fastphase" falsevalue="" type="boolean" checked="false" label="Output genotypes in fastPHASE format" />
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244 <param argument="--plink" truevalue="--plink" falsevalue="" type="boolean" checked="false" label="Output genotypes in PLINK format" />
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245 <param argument="--hzar" truevalue="--hzar" falsevalue="" type="boolean" checked="false" label="Output genotypes in Hybrid Zone Analysis using R (HZAR) format." />
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246 <param argument="--phylip" truevalue="--phylip" falsevalue="" type="boolean" checked="false" label="Output nucleotides that are fixed-within, and variant among populations in Phylip format for phylogenetic tree construction" />
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247 <param argument="--phylip_var" truevalue="--phylip_var" falsevalue="" type="boolean" checked="false" label="Include variable sites in the phylip output encoded using IUPAC notation." />
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248 <!--<param argument="\-\-phylip_var_all" truevalue="\-\-phylip_var_all" falsevalue="" type="boolean" checked="false" label="Include all sequence as well as variable sites in the phylip output encoded using IUPAC notation." />-->
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249 <param argument="--treemix" truevalue="--treemix" falsevalue="" type="boolean" checked="false" label="Output SNPs in a format useable for the TreeMix program (Pickrell and Pritchard)." />
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250 <param argument="--no_hap_exports" truevalue="--no_hap_exports" falsevalue="" type="boolean" checked="false" label="Omit haplotype outputs" />
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251 <param argument="--fasta_samples_raw" truevalue="--fasta_samples_raw" falsevalue="" type="boolean" checked="false" label="Output all haplotypes observed in each sample, for each locus, in FASTA format" />
0
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252 </section>
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253
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254 <!-- Kernel-smoothing algorithm -->
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255 <section name="kernel_smoothing" title="Kernel smoothing" expanded="false">
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256 <conditional name="options_kernel">
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257 <param name="kernel" type="select" label="Enable kernel-smoothed calculations" >
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258 <option value="">No</option>
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259 <option value="-k">For Pi, Fis, Fst, Fst', and Phi_st (-k)</option>
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260 <option value="--smooth_fstats">For Fst, Fst', and Phi_st (--smooth_fstats)</option>
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261 <option value="--smooth_popstats">For Pi and Fis (--smooth_popstats)</option>
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262 </param>
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263 <when value=""/>
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264 <when value="-k">
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265 <param argument="--sigma" type="float" value="150000" label="Standard deviation of the kernel smoothing weight distribution" help="distance over which to average values (default 150Kb)" />
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266 </when>
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267 <when value="--smooth_fstats">
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268 <param argument="--sigma" type="float" value="150000" label="Standard deviation of the kernel smoothing weight distribution" help="distance over which to average values (default 150Kb)" />
0
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269 </when>
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270 <when value="--smooth_popstats">
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271 <param argument="--sigma" type="float" value="150000" label="Standard deviation of the kernel smoothing weight distribution" help="distance over which to average values (default 150Kb)" />
0
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272 </when>
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273 </conditional>
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274 <conditional name="bootstrap_resampling_mode">
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275 <param argument="--bootstrap" type="select" label="Enable bootstrap resampling for all smoothed statistics" >
0
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276 <option value="">No</option>
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277 <option value="--bootstrap">Yes</option>
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278 </param>
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279 <when value="--bootstrap">
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280 </when>
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281 <when value="">
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282 <param argument="--bootstrap_pifis" type="boolean" checked="false" truevalue="--bootstrap_pifis" falsevalue="" label="Enable boostrap resampling for smoothed SNP-based Pi and Fis calculations" />
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283 <param argument="--bootstrap_fst" type="boolean" checked="false" truevalue="--bootstrap_fst" falsevalue="" label="Enable boostrap resampling for smoothed Fst calculations based on pairwise population comparison of SNPs" />
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284 <param argument="--bootstrap_div" type="boolean" checked="false" truevalue="--bootstrap_div" falsevalue="" label="Enable boostrap resampling for smoothed haplotype diveristy and gene diversity calculations based on haplotypes" />
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285 <param argument="--bootstrap_phist" type="boolean" checked="false" truevalue="--bootstrap_phist" falsevalue="" label="Enable boostrap resampling for smoothed Phi_st calculations based on haplotypes." />
0
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286 </when>
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287 </conditional>
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288 <param argument="--bootstrap_reps" type="integer" value="100" optional="true" label="Number of bootstrap resamplings to calculate" />
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289 <param argument="--bootstrap_wl" format="txt,tabular" type="data" optional="true" label="Only bootstrap loci contained in this whitelist" />
0
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290 </section>
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291
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292 <!-- Output options -->
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293 <section name="advanced_options" title="Advanced options" expanded="False">
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294 <param argument="--log_fst_comp" type="boolean" checked="false" truevalue="--log_fst_comp" falsevalue="" label="Log components of Fst/Phi_st calculations to a dataset" />
0
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295 </section>
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296 <expand macro="in_log"/>
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297 </inputs>
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298 <outputs>
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299 <expand macro="out_log"/>
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300 <expand macro="populations_output_full"/>
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301 </outputs>
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302
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303 <tests>
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304 <!-- test w gstacks output as input and default options -->
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305 <test>
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306 <param name="options_usage|input_type" value="stacks" />
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307 <param name="options_usage|input_aln">
0
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308 <collection type="list">
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309 <element name="catalog.calls.vcf" ftype="vcf" value="gstacks/catalog.calls.vcf" />
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310 <element name="catalog.fa.gz" ftype="fasta.gz" value="gstacks/catalog.fa.gz" />
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311 </collection>
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312 </param>
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313 <param name="popmap" ftype="tabular" value="denovo_map/popmap_cstacks.tsv" />
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314 <param name="add_log" value="yes" />
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315 <output ftype="txt" name="output_log" value="populations/populations.log" lines_diff="4"/>
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316 <output ftype="tabular" name="out_haplotypes" value="populations/populations.haplotypes.tsv"/>
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317 <output ftype="tabular" name="out_hapstats" value="populations/populations.hapstats.tsv"/>
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318 <output ftype="txt" name="out_populations_log_distribs" value="populations/populations.log.distribs"/>
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319 <output ftype="tabular" name="out_sumstats_sum" value="populations/populations.sumstats_summary.tsv"/>
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320 <output ftype="tabular" name="out_sumstats" value="populations/populations.sumstats.tsv"/>
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321 <output ftype="tabular" name="out_sql" value="populations/populations.markers.tsv"/>
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322 </test>
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323 <!-- test w gstacks output as input, wo popmap, and default options -->
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324 <test>
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325 <param name="options_usage|input_type" value="stacks" />
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326 <param name="options_usage|input_aln">
0
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327 <collection type="list">
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328 <element name="catalog.calls.vcf" ftype="vcf" value="gstacks/catalog.calls.vcf" />
0
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329 <element name="catalog.fa.gz" ftype="fasta.gz" value="gstacks/catalog.fa.gz" />
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330 </collection>
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331 </param>
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332 <param name="add_log" value="yes" />
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333 <output ftype="txt" name="output_log"><assert_contents><has_text text="Populations is done." /></assert_contents></output>
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334 <output ftype="tabular" name="out_haplotypes"><assert_contents><has_text text="" /></assert_contents></output>
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335 <output ftype="tabular" name="out_hapstats"><assert_contents><has_text text="" /></assert_contents></output>
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336 <output ftype="txt" name="out_populations_log_distribs"><assert_contents><has_text text="" /></assert_contents></output>
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337 <output ftype="tabular" name="out_sumstats_sum"><assert_contents><has_text text="" /></assert_contents></output>
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338 <output ftype="tabular" name="out_sumstats"><assert_contents><has_text text="" /></assert_contents></output>
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339 <output ftype="tabular" name="out_sql"><assert_contents><has_text text="" /></assert_contents></output>
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340 </test>
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341 <!-- test w gstacks output as input and + all outputs -->
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342 <test>
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343 <param name="options_usage|input_type" value="stacks" />
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344 <param name="options_usage|input_aln">
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345 <collection type="list">
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346 <element name="catalog.calls.vcf" ftype="vcf" value="gstacks/catalog.calls.vcf" />
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347 <element name="catalog.fa.gz" ftype="fasta.gz" value="gstacks/catalog.fa.gz" />
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348 </collection>
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349 </param>
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350 <param name="popmap" ftype="tabular" value="denovo_map/popmap_cstacks.tsv" />
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351 <param name="add_log" value="yes" />
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352 <param name="advanced_options|log_fst_comp" value="yes"/>
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353 <param name="fstats_conditional|fstats" value="yes"/>
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354 <param name="populations_output|fasta_loci" value="yes"/>
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355 <param name="populations_output|fasta_samples" value="yes"/>
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356 <param name="populations_output|fasta_samples_raw" value="yes"/>
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357 <param name="populations_output|phylip" value="yes"/>
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358 <param name="populations_output|phylip_var" value="yes"/>
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359 <param name="populations_output|genepop" value="yes"/>
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360 <param name="populations_output|vcf" value="yes"/>
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361 <param name="populations_output|hzar" value="yes"/>
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362 <param name="populations_output|plink" value="yes"/>
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363 <param name="populations_output|structure" value="yes"/>
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364 <param name="populations_output|radpainter" value="yes"/>
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365 <param name="populations_output|treemix" value="yes"/>
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366 <assert_command>
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367 <has_text text="--log_fst_comp" />
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368 </assert_command>
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369 <output ftype="txt" name="output_log"><assert_contents><has_text text="Populations is done." /></assert_contents></output>
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370 <output ftype="tabular" name="out_haplotypes" value="populations/populations.haplotypes.tsv"/>
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371 <output ftype="tabular" name="out_hapstats" value="populations/populations.hapstats.tsv"/>
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372 <output ftype="txt" name="out_populations_log_distribs" value="populations/populations.log.distribs" lines_diff="2"/>
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373 <output ftype="tabular" name="out_sumstats_sum" value="populations/populations.sumstats_summary.tsv"/>
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374 <output ftype="tabular" name="out_sumstats" value="populations/populations.sumstats.tsv"/>
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375 <output ftype="tabular" name="out_sql" value="populations/populations.markers.tsv"/>
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376
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377 <output ftype="tabular" name="out_phistats" value="populations/populations.phistats.tsv"/>
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378 <output ftype="tabular" name="out_phistats_sum" value="populations/populations.phistats_summary.tsv"/>
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379 <output ftype="tabular" name="out_fststats_sum" value="populations/populations.fst_summary.tsv"/>
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380 <output ftype="tabular" name="out_fasta_strict" value="populations/populations.loci.fa" compare="sim_size" delta="50"/><!--there seems to be reordering of the lines, so differences are expected only in the date contained in the Header -->
1e260cccfafb planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f507c4eaca0fe04e730d7c3bbb9e9d2488239a9f
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381 <output ftype="tabular" name="out_fasta" value="populations/populations.samples.fa" compare="sim_size" delta="50"/><!-- " -->
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382 <output ftype="tabular" name="out_fasta_raw" value="populations/populations.samples-raw.fa" compare="sim_size" delta="50"/><!-- " -->
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383 <output ftype="tabular" name="out_phylip_all_pop_fix" value="populations/populations.fixed.phylip" lines_diff="2"/><!-- " -->
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384 <output ftype="tabular" name="out_phylip_all_loci_fix" value="populations/populations.fixed.phylip.log" lines_diff="2"/>
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385 <output ftype="tabular" name="out_phylip_all_pop_var" value="populations/populations.var.phylip" compare="sim_size" delta="50"/>
1e260cccfafb planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f507c4eaca0fe04e730d7c3bbb9e9d2488239a9f
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386 <output ftype="tabular" name="out_phylip_all_loci_var" value="populations/populations.var.phylip.log" compare="sim_size" delta="50"/><!--there seems to be reordering of the lines, so differences are expected only in the date contained in the Header -->
1e260cccfafb planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f507c4eaca0fe04e730d7c3bbb9e9d2488239a9f
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387 <output ftype="tabular" name="out_genepop_snps" value="populations/populations.snps.genepop" compare="sim_size" delta="50"/><!-- " -->
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388 <output ftype="tabular" name="out_genepop_haps" value="populations/populations.haps.genepop" compare="sim_size" delta="50"/><!-- " -->
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389 <output ftype="vcf" name="out_vcf_haplotypes_snps" value="populations/populations.snps.vcf" compare="sim_size" delta="50"/><!-- " -->
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390 <output ftype="vcf" name="out_vcf_haplotypes_haps" value="populations/populations.haps.vcf" compare="sim_size" delta="50"/><!-- " -->
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391 <output ftype="tabular" name="out_plink_markers" value="populations/populations.plink.map" compare="sim_size" delta="50"/><!-- " -->
1e260cccfafb planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f507c4eaca0fe04e730d7c3bbb9e9d2488239a9f
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392 <output ftype="tabular" name="out_plink_genotypes" value="populations/populations.plink.ped" compare="sim_size" delta="50"/><!-- " -->
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393 <output ftype="tabular" name="out_hzar" value="populations/populations.hzar.csv" compare="sim_size" delta="50"/><!-- " -->
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394 <output ftype="tabular" name="out_structure" value="populations/populations.structure" compare="sim_size" delta="50"/><!-- " -->
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395 <output ftype="tabular" name="out_radpainter" value="populations/populations.haps.radpainter" compare="sim_size" delta="50"/><!-- " -->
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396 <output ftype="tabular" name="out_treemix" value="populations/populations.treemix" compare="sim_size" delta="50"/><!-- " -->
0
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397 </test>
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398 <!-- test w vcf input and default options, just checking if finished -->
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399 <test>
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400 <param name="options_usage|input_type" value="vcf" />
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401 <param name="options_usage|input_vcf" ftype="vcf" value="gstacks/catalog.calls.vcf"/>
0
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402 <param name="popmap" ftype="tabular" value="denovo_map/popmap_cstacks.tsv" />
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403 <param name="add_log" value="yes" />
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404 <output ftype="txt" name="output_log"><assert_contents><has_text text="Populations is done." /></assert_contents></output>
5
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405 <output ftype="tabular" name="out_haplotypes"><assert_contents><has_text text="" /></assert_contents></output>
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406 <output ftype="tabular" name="out_hapstats"><assert_contents><has_text text="" /></assert_contents></output>
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407 <output ftype="txt" name="out_populations_log_distribs"><assert_contents><has_text text="" /></assert_contents></output>
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408 <output ftype="tabular" name="out_sumstats_sum"><assert_contents><has_text text="" /></assert_contents></output>
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409 <output ftype="tabular" name="out_sumstats"><assert_contents><has_text text="" /></assert_contents></output>
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410 <output ftype="tabular" name="out_sql"><assert_contents><has_text text="" /></assert_contents></output>
0
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411 </test>
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412 <!-- test w gstacks output as input and non default filtering -->
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413 <test>
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414 <param name="options_usage|input_type" value="stacks" />
4
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415 <param name="options_usage|input_aln">
0
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416 <collection type="list">
5
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417 <element name="catalog.calls.vcf" ftype="vcf" value="gstacks/catalog.calls.vcf" />
0
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418 <element name="catalog.fa.gz" ftype="fasta.gz" value="gstacks/catalog.fa.gz" />
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419 </collection>
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420 </param>
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421 <param name="popmap" ftype="tabular" value="denovo_map/popmap_cstacks.tsv" />
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422 <param name="add_log" value="yes" />
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423 <section name="options_filtering">
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424 <param name="minperc" value="0.1"/>
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425 <param name="minpop" value="2"/>
5
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426 <param name="min_samples_overall" value="0.01"/>
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427 <param name="filter_haplotype_wise" value="--filter-haplotype-wise"/>
6
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428 <param name="min_maf" value="0.01" />
0
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429 <param name="min_mac" value="1"/>
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430 <param name="max_obs_het" value="0.8" />
5
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431 <param name="blacklist" value="populations/blacklist.tsv" ftype="tabular" />
0
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432 </section>
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433 <assert_command>
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434 <has_text text="-r 0.1" />
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435 <has_text text="-p 2" />
5
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436 <has_text text="--min-samples-overall 0.01" />
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437 <has_text text="--filter-haplotype-wise" />
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438 <has_text text="--min_maf 0.01" />
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439 <has_text text="--min_mac 1" />
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440 <has_text text="--max_obs_het 0.8" />
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441 <has_text text="-B " />
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442 </assert_command>
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443 <output ftype="txt" name="output_log"><assert_contents><has_text text="Populations is done." /></assert_contents></output>
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444 <output ftype="tabular" name="out_haplotypes"><assert_contents><has_text text="" /></assert_contents></output>
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445 <output ftype="tabular" name="out_hapstats"><assert_contents><has_text text="" /></assert_contents></output>
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446 <output ftype="txt" name="out_populations_log_distribs"><assert_contents><has_text text="" /></assert_contents></output>
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447 <output ftype="tabular" name="out_sumstats_sum"><assert_contents><has_text text="" /></assert_contents></output>
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448 <output ftype="tabular" name="out_sumstats"><assert_contents><has_text text="" /></assert_contents></output>
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449 <output ftype="tabular" name="out_sql"><assert_contents><has_text text="" /></assert_contents></output>
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diff changeset
450 </test>
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451 <!-- test w gstacks output as input and non default filtering (needed because filter-haplotype-wise is incompatible with write_random_snp/write_single_snp) -->
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diff changeset
452 <test>
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diff changeset
453 <param name="options_usage|input_type" value="stacks" />
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diff changeset
454 <param name="options_usage|input_aln">
35e673d79262 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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diff changeset
455 <collection type="list">
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456 <element name="catalog.calls.vcf" ftype="vcf" value="gstacks/catalog.calls.vcf" />
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457 <element name="catalog.fa.gz" ftype="fasta.gz" value="gstacks/catalog.fa.gz" />
35e673d79262 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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458 </collection>
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diff changeset
459 </param>
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460 <param name="popmap" ftype="tabular" value="denovo_map/popmap_cstacks.tsv" />
35e673d79262 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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461 <param name="add_log" value="yes" />
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diff changeset
462 <section name="options_filtering">
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463 <param name="minperc" value="0.1"/>
35e673d79262 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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464 <param name="minpop" value="2"/>
35e673d79262 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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465 <param name="min_samples_overall" value="0.01"/>
6
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466 <param name="min_maf" value="0.01" />
5
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diff changeset
467 <param name="min_mac" value="1"/>
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468 <param name="max_obs_het" value="0.8" />
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469 <!-- since write_random_snp runs in ifinite loop switch to write_single -->
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470 <param name="filter_single_snp" value="--write_single_snp"/>
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471 <param name="blacklist" value="populations/blacklist.tsv" ftype="tabular" />
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472 </section>
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473 <assert_command>
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474 <has_text text="-r 0.1" />
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diff changeset
475 <has_text text="-p 2" />
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476 <has_text text="--min-samples-overall 0.01" />
0
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477 <has_text text="--min_maf 0.01" />
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478 <has_text text="--min_mac 1" />
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479 <has_text text="--max_obs_het 0.8" />
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480 <has_text text="--write_random_snp" />
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481 <has_text text="-B " />
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482 </assert_command>
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483 <output ftype="txt" name="output_log"><assert_contents><has_text text="Populations is done." /></assert_contents></output>
5
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484 <output ftype="tabular" name="out_haplotypes"><assert_contents><has_text text="" /></assert_contents></output>
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485 <output ftype="tabular" name="out_hapstats"><assert_contents><has_text text="" /></assert_contents></output>
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486 <output ftype="txt" name="out_populations_log_distribs"><assert_contents><has_text text="" /></assert_contents></output>
35e673d79262 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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parents: 4
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487 <output ftype="tabular" name="out_sumstats_sum"><assert_contents><has_text text="" /></assert_contents></output>
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488 <output ftype="tabular" name="out_sumstats"><assert_contents><has_text text="" /></assert_contents></output>
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489 <output ftype="tabular" name="out_sql"><assert_contents><has_text text="" /></assert_contents></output>
0
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490 </test>
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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491 <!-- test w gstacks output as input and non default merging/hwe -->
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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492 <test>
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493 <param name="options_usage|input_type" value="stacks" />
4
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494 <param name="options_usage|input_aln">
0
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495 <collection type="list">
5
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496 <element name="catalog.calls.vcf" ftype="vcf" value="gstacks/catalog.calls.vcf" />
0
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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497 <element name="catalog.fa.gz" ftype="fasta.gz" value="gstacks/catalog.fa.gz" />
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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498 </collection>
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499 </param>
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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500 <param name="popmap" ftype="tabular" value="denovo_map/popmap_cstacks.tsv" />
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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501 <section name="merge_phase">
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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502 <param name="enzyme" value="ecoRI"/>
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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503 <param name="merge_sites" value="yes"/>
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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504 <param name="merge_prune_lim" value="0.9" />
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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505 </section>
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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diff changeset
506 <section name="locus_stats">
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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507 <param name="hwe" value="yes" />
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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diff changeset
508 </section>
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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509 <param name="add_log" value="yes" />
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
matthias
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diff changeset
510 <assert_command>
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511 <has_text text="-e ecoRI" />
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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512 <has_text text="--merge_sites" />
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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513 <has_text text="--merge_prune_lim 0.9" />
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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514 <has_text text="--hwe" />
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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515 </assert_command>
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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516 <output ftype="txt" name="output_log"><assert_contents><has_text text="Populations is done." /></assert_contents></output>
5
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517 <output ftype="tabular" name="out_haplotypes"><assert_contents><has_text text="" /></assert_contents></output>
35e673d79262 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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518 <output ftype="tabular" name="out_hapstats"><assert_contents><has_text text="" /></assert_contents></output>
35e673d79262 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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519 <output ftype="txt" name="out_populations_log_distribs"><assert_contents><has_text text="" /></assert_contents></output>
35e673d79262 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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520 <output ftype="tabular" name="out_sumstats_sum"><assert_contents><has_text text="" /></assert_contents></output>
35e673d79262 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
matthias
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521 <output ftype="tabular" name="out_sumstats"><assert_contents><has_text text="" /></assert_contents></output>
35e673d79262 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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522 <output ftype="tabular" name="out_sql"><assert_contents><has_text text="" /></assert_contents></output>
35e673d79262 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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523 <output ftype="txt" name="output_log"><assert_contents><has_text text="Populations is done." /></assert_contents></output>
0
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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diff changeset
524 </test>
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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diff changeset
525 <!-- test w gstacks output as input and non default fstats/kernel/bootstrap -->
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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diff changeset
526 <test>
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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diff changeset
527 <param name="options_usage|input_type" value="stacks" />
4
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diff changeset
528 <param name="options_usage|input_aln">
0
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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diff changeset
529 <collection type="list">
5
35e673d79262 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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diff changeset
530 <element name="catalog.calls.vcf" ftype="vcf" value="refmap/catalog.calls.vcf" />
35e673d79262 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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diff changeset
531 <element name="catalog.fa.gz" ftype="fasta.gz" value="refmap/catalog.fa.gz" />
0
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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diff changeset
532 </collection>
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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diff changeset
533 </param>
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
matthias
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diff changeset
534 <param name="popmap" ftype="tabular" value="denovo_map/popmap_cstacks.tsv" />
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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parents:
diff changeset
535 <conditional name="fstats_conditional">
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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diff changeset
536 <param name="fstats" value="yes" />
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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diff changeset
537 <conditional name="correction_select">
6
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diff changeset
538 <param name="fst_correction" value="bonferroni_gen"/>
1e260cccfafb planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f507c4eaca0fe04e730d7c3bbb9e9d2488239a9f
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diff changeset
539 <param name="p_value_cutoff" value="0.01" />
0
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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diff changeset
540 </conditional>
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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diff changeset
541 </conditional>
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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diff changeset
542 <conditional name="options_kernel">
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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diff changeset
543 <param name="kernel" value="-k" />
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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diff changeset
544 <param name="sigma" value="150001" />
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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diff changeset
545 </conditional>
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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diff changeset
546 <conditional name="bootstrap_resampling_mode">
6
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diff changeset
547 <param name="bootstrap" value="--bootstrap" />
0
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548 </conditional>
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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diff changeset
549 <param name="bootstrap_reps" value="23" />
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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diff changeset
550 <param name="add_log" value="yes" />
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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diff changeset
551 <assert_command>
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diff changeset
552 <has_text text="--fstats" />
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
matthias
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diff changeset
553 <has_text text="--fst_correction bonferroni_gen" />
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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diff changeset
554 <has_text text="--p_value_cutoff 0.01" />
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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diff changeset
555 <has_text text="-k " />
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
matthias
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diff changeset
556 <has_text text="--sigma 150001" />
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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diff changeset
557 <has_text text="--bootstrap" />
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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diff changeset
558 <has_text text="--bootstrap_reps 23" />
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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diff changeset
559 </assert_command>
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diff changeset
560 <output ftype="txt" name="output_log"><assert_contents><has_text text="Populations is done." /></assert_contents></output>
5
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parents: 4
diff changeset
561 <output ftype="txt" name="output_log"><assert_contents><has_text text="Populations is done." /></assert_contents></output>
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parents: 4
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562 <output ftype="tabular" name="out_haplotypes"><assert_contents><has_text text="" /></assert_contents></output>
35e673d79262 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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parents: 4
diff changeset
563 <output ftype="tabular" name="out_hapstats"><assert_contents><has_text text="" /></assert_contents></output>
35e673d79262 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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parents: 4
diff changeset
564 <output ftype="txt" name="out_populations_log_distribs"><assert_contents><has_text text="" /></assert_contents></output>
35e673d79262 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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parents: 4
diff changeset
565 <output ftype="tabular" name="out_sumstats_sum"><assert_contents><has_text text="" /></assert_contents></output>
35e673d79262 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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parents: 4
diff changeset
566 <output ftype="tabular" name="out_sumstats"><assert_contents><has_text text="" /></assert_contents></output>
35e673d79262 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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parents: 4
diff changeset
567 <output ftype="tabular" name="out_sql"><assert_contents><has_text text="" /></assert_contents></output>
0
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568 </test>
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569 </tests>
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570 <help>
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571 <![CDATA[
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572 .. class:: infomark
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573
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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574 **What it does**
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575
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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576 This program will be executed in place of the genotypes program when a population is being processed through the pipeline. A map specifiying which individuals belong to which population is submitted to the program and the program will then calculate population genetics statistics, expected/observed heterzygosity, π, and FIS at each nucleotide position. The populations program will compare all populations pairwise to compute FST. If a set of data is reference aligned, then a kernel-smoothed FST will also be calculated.
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577
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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578 --------
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579
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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580 **Input files**
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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581
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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582 Output from denovo_map or ref_map
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583
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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584 - Population map::
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585
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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586 indv_01 1
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587 indv_02 1
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588 indv_03 1
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589 indv_04 2
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590 indv_05 2
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591 indv_06 2
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592
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593
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594 **Output files**
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595
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596 - XXX.tags.tsv file
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597
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598 See `Stacks output description <http://catchenlab.life.illinois.edu/stacks/manual/#files>`_
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599
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600 Notes: For the tags file, each stack will start in the file with a consensus sequence for the entire stack followed by the flags for that stack. Then, each individual read that was merged into that stack will follow. The next stack will start with another consensus sequence.
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601
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602
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603 - XXX.snps.tsv file
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604
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605 See `Stacks output description <http://catchenlab.life.illinois.edu/stacks/manual/#files>`_
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606
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607 Notes: If a stack has two SNPs called within it, then there will be two lines in this file listing each one.
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608
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609
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610 - XXX.alleles.tsv file
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611
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612 See `Stacks output description <http://catchenlab.life.illinois.edu/stacks/manual/#files>`_
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613
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614
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615 - XXX.matches.tsv file
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616
3950b915b7cf planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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617 See `Stacks output description <http://catchenlab.life.illinois.edu/stacks/manual/#files>`_
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618
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619 Notes: Each line in this file records a match between a catalog locus and a locus in an individual, for a particular haplotype. The Batch ID plus the Catalog ID together represent a unique locus in the entire population, while the Sample ID and the Stack ID together represent a unique locus in an individual sample.
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620
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621
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622 - other files:
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623
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624 See `Stacks output description <http://catchenlab.life.illinois.edu/stacks/manual/#files>`_
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625
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626 @STACKS_INFOS@
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627 ]]>
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628 </help>
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629 <expand macro="citation" />
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630 </tool>