Mercurial > repos > iuc > wfmash
diff wfmash.xml @ 0:8534f4965ac5 draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/wfmash commit db025e5d190c805c537e4c8ad7682bf5fdd946c5
| author | iuc |
|---|---|
| date | Mon, 09 Feb 2026 12:40:35 +0000 |
| parents | |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/wfmash.xml Mon Feb 09 12:40:35 2026 +0000 @@ -0,0 +1,65 @@ +<tool id="wfmash" name="wfmash" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@"> + <description>pangenome-scale aligner</description> + <macros> + <import>macros.xml</import> + </macros> + <expand macro="requirements"/> + <command detect_errors="exit_code"><![CDATA[ +#set $reference_fasta_filename = "reference.fa" +#set $query_fasta_filename = "query.fa" + +#if str($reference.ext).endswith('gz') + gunzip -c '${reference}' > '${reference_fasta_filename}' && +#else + ln -s -f '${reference}' '${reference_fasta_filename}' && +#end if + + samtools faidx '${reference_fasta_filename}' && + +#if str($query.ext).endswith('gz') +gunzip -c '${query}' > '${query_fasta_filename}' && +#else +ln -s -f '${query}' '${query_fasta_filename}' && +#end if +samtools faidx '${query_fasta_filename}' && + +wfmash + --threads="\${GALAXY_SLOTS:-1}" + -s $segment_length + -p $pct_identity + $approx_mapping + $no_split + '${reference_fasta_filename}' + '${query_fasta_filename}' + > '${output_paf}' + ]]></command> + <inputs> + <param name="reference" type="data" format="fasta,fasta.gz" label="Reference sequence" help="FASTA format" /> + <param name="query" type="data" format="fasta,fasta.gz" label="Query sequence" help="FASTA format"/> + <param argument="-s" name="segment_length" type="integer" min="100" value="1000" label="Segment Length" help="Minimum seed length, must be >= 100 bp."/> + <param argument="-p" name="pct_identity" type="float" min="0" max="100" value="90" label="Minimum Identity %" help="Percent identity in the mashmap step"/> + <param argument="-m" name="approx_mapping" type="boolean" truevalue="-m" falsevalue="" label="Approximate mapping" help="Skip base-level alignment"/> + <param argument="-N" name="no_split" type="boolean" optional="true" truevalue="-N" falsevalue="" label="No split" help="Disable splitting of input sequences during mapping (Default: enabled)"/> + </inputs> + + <outputs> + <data name="output_paf" format="paf" label="${tool.name} on ${on_string}: PAF"/> + </outputs> + + <tests> + <test expect_num_outputs="1"> + <param name="reference" ftype="fasta" value="ecoli_k12.fa"/> + <param name="query" ftype="fasta" value="ecoli_o157.fa"/> + <param name="pct_identity" value="70"/> + <output name="output_paf" file="expected_output_ecoli.paf" sort="true"/> + </test> + </tests> + + <help><![CDATA[ +wfmash is designed to make whole genome alignment easy. It can handle high sequence divergence. + +By default, wfmash automatically determines an appropriate identity threshold based on the ANI (Average Nucleotide Identity) distribution of your input sequences. + ]]></help> + + <expand macro="citations"/> +</tool>
