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annotate rtsne.xml @ 0:ac9e08add934 draft default tip

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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
author iuc
date Tue, 22 Aug 2017 17:48:33 -0400
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ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
iuc
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1 <tool id="tsne" name="rtsne" version="0.0.2">
ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
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2 <requirements>
ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
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3 <requirement type="package" version="0.13">r-rtsne</requirement>
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4 <requirement type="package" version="2.2.1">r-ggplot2</requirement>
ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
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5 </requirements>
ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
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6 <command detect_errors="exit_code"><![CDATA[
ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
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7 cat '$script' &&
ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
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8 Rscript '$script' &&
ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
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9 mv output_plot.pdf $output1
ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
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10 ]]></command>
ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
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11 <configfiles>
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12 <configfile name="script"><![CDATA[
ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
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13 ## Setup R error handling to go to stderr
ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
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14 options(show.error.messages=F, error=function(){cat(geterrmessage(),file=stderr());q("no",1,F)})
ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
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15 loc <- Sys.setlocale("LC_MESSAGES", "en_US.UTF-8")
ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
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16
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17 ## Import library
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18 library(Rtsne)
ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
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19 library(ggplot2)
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ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
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21 all <- read.delim('$input1', sep='\t', header=TRUE)
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22
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23 data = $data
ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
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24 name = $name
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25
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26 #if $transform == "none"
ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
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27 log.all <- all[, data:ncol(all)]
ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
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28 #elif $transform == "log2"
ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
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29 log.all <- log2(all[, data:ncol(all)])
ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
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30 #elsif $transform == "log2plus1"
ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
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31 log.all <- log2(1+all[, data:ncol(all)])
ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
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32 #elif $transform == "log10"
ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
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33 log.all <- log(all[, data:ncol(all)])
ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
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34 #elif $transform == "log10plus1"
ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
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35 log.all <- log(1+all[, data:ncol(all)])
ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
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36 #end if
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37
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38 ## Show/hide legend
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39 #if $legend == "yes"
ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
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40 gg_legend = NULL
ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
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41 #else
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42 gg_legend = theme(legend.position="none")
ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
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43 #end if
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44
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45 set.seed($seed) ## Sets seed for reproducibility
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46 tsne_out <- Rtsne(as.matrix(log.all[,1:ncol(log.all)]), perplexity=$perp, theta=$theta) ## Run TSNE
ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
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47
ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
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48 embedding <- as.data.frame(tsne_out\$Y)
ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
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49 embedding\$Class <- as.factor(sub("Class_", "", all[,name]))
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50
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51 ggplot(embedding, aes(x=V1, y=V2, color=Class)) +
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52 geom_point(size=1.25) +
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53 gg_legend +
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54 xlab("") +
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55 ylab("") +
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56 ggtitle('$title')
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57
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58 ggsave(file='output_plot.pdf')
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59 dev.off()
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60
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61 ]]></configfile>
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62 </configfiles>
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63
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64 <inputs>
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65 <param name="input1" type="data" format="tabular"
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66 label="Tabular input dataset"
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67 help="Input should have column headers with an identifier column followed by consencutive numerical columns used for clustering"/>
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68 <param name="title" type="text" format="txt" label="Plot title"/>
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69 <param name="seed" value="42.0" type="float" label="Seed value for reproducibiity" help="Set to 42 as default" />
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70 <param name="perp" value="10.0" type="float" label="perplexity"/>
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71 <param name="theta" value="1.0" type="float" label="theta"/>
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72 <param name="name" value="1" type="data_column" data_ref="input1"
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73 label="The column with the ID to use in grouping (or just an ID if plotting invdividuals)"/>
ac9e08add934 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tsne commit 9820a6eac7deb86db27eb85ba756ce0267c178e8
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74 <param name="data" value="2" type="data_column" data_ref="input1"
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75 label="The column that contains the first numerical data"/>
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76 <param name="transform" type="select" label="Advanced - log transformation">
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77 <option value="none">Plot the data as it is</option>
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78 <option value="log2">Log2(value) transform my data</option>
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79 <option value="log2plus1">Log2(value+1) transform my data</option>
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80 <option value="log10">Log10(value) transform my data</option>
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81 <option value="log10plus1">Log10(value+1) transform my data</option>
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82 </param>
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83
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84 <param name="legend" type="select" label="Legend options">
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85 <option value="yes">Include legend on plot</option>
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86 <option value="no">Hide legend</option>
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87 </param>
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88 </inputs>
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89 <outputs>
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90 <data name="output1" format="pdf" from_work_dir="Rplot.pdf"/>
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91 </outputs>
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92 <tests>
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93 <test>
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94 <param name="input1" value="mtcars.txt"/>
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95 <param name="transform" value="none"/>
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96 <param name="perp" value="10.0"/>
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97 <output name="output1" file="Rplot.pdf"/>
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98 </test>
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99 </tests>
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100 <help><![CDATA[
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101 T-distributed Stochastic Neighbor Embedding implementation by Van der Maaten (see <https://github.com/lvdmaaten/bhtsne/>
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102 for more information on the original implementation).
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103
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104 Your data should be in tabular format. Objects in rows will be clustered according to the observations in columns.
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105 Labels for objects can be assigned in the tool form by providing the column number of the identifier you wish to use.
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106 Additionaly, the first column containing numeric data should be provided to the tool form.
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107
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108 ]]></help>
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109 <citations>
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110 <citation type="bibtex">
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111 @Manual{,
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112 title = {{Rtsne}: T-Distributed Stochastic Neighbor Embedding using
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113 Barnes-Hut Implementation},
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114 author = {Jesse H. Krijthe},
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115 year = {2015},
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116 note = {R package version 0.13},
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117 url = {https://github.com/jkrijthe/Rtsne},
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118 }
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119 </citation>
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120 </citations>
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121 </tool>