annotate stacks_rxstacks.xml @ 9:3d55098d428e draft default tip

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks commit 74ee33c6e30744a6da8deb7116d431d80ee80edb
author iuc
date Fri, 07 Apr 2023 21:56:33 +0000
parents 571245acc8a4
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1 <tool id="stacks_rxstacks" name="Stacks: rxstacks" version="@WRAPPER_VERSION@.0">
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2 <description>make corrections to genotype and haplotype calls</description>
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3 <macros>
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4 <import>macros.xml</import>
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5 </macros>
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6 <expand macro="bio_tools"/>
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7 <expand macro="requirements"/>
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8 <expand macro="stdio"/>
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9 <command><![CDATA[
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11 mkdir stacks_inputs stacks_outputs
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13 &&
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5
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15 #for $input_file in $input_col
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16 #set $ext = ""
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17 #if not str($input_file.element_identifier).endswith('.tsv')
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18 #set $ext = ".tsv"
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19 #end if
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20 ln -s '${input_file}' 'stacks_inputs/${input_file.element_identifier}${ext}' &&
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21 #end for
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23 rxstacks
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25 -t \${GALAXY_SLOTS:-1}
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26
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27 -P stacks_inputs
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29 ## Batch description
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30 -b 1
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31
5
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32 #if str($options_filtering.lnl)
0
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33 --lnl_filter
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34 --lnl_lim $options_filtering.lnl
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35 #end if
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36
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37 $options_filtering.lnl_dist
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38
5
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39 #if str($options_filtering.conf)
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40 --conf_filter
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41 --conf_lim $options_filtering.conf
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42 #end if
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43
5
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44 #if $options_filtering.prune.prune_haplo == "yes"
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45 --prune_haplo
5
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46 #if str($options_filtering.prune.max_haplo)
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47 --max_haplo $options_filtering.prune.max_haplo
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48 #end if
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49 #end if
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50
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51 ## snp_model
5
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52 #if str( $snp_options.select_model.model_type) == "bounded"
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53 --model_type bounded
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54 --bound_low $snp_options.select_model.bound_low
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55 --bound_high $snp_options.select_model.bound_high
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56 --alpha $snp_options.select_model.alpha
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57 #else if str( $snp_options.select_model.model_type) == "snp"
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58 --model_type snp
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59 --alpha $snp_options.select_model.alpha
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60 #else
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61 --model_type fixed
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62 #end if
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63
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64 -o stacks_outputs
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65 ]]></command>
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66
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67 <inputs>
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68 <param name="input_col" format="tabular,txt" type="data_collection" collection_type="list" label="Output from previous Stacks pipeline steps (e.g. denovo_map or refmap)" />
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69
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70 <section name="options_filtering" title="Data filtering options" expanded="true">
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71 <param name="lnl" type="float" value="" optional="true" argument="--lnl_lim" label="Filter loci with log likelihood values below this threshold" />
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72 <param argument="--lnl_dist" truevalue="--lnl_dist" falsevalue="" type="boolean" checked="false" label="Print distribution of mean log likelihoods for catalog loci." />
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73
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74 <param name="conf" type="float" value="0.75" optional="true" argument="--conf_lim" min="0.0" max="1.0" label="Proportion of loci in population that must be confounded relative to the catalog locus (between 0.0 and 1.0)."/>
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75
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76 <conditional name="prune">
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77 <param argument="--prune_haplo" type="select" label="Prune out non-biological haplotypes unlikely to occur in the population.">
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78 <option value="no" selected="true">No</option>
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79 <option value="yes">Yes</option>
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80 </param>
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81 <when value="no"/>
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82 <when value="yes">
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83 <param argument="--max_haplo" type="integer" value="" optional="true" label="Only consider haplotypes for pruning if they occur in fewer than this value."/>
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84 </when>
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85 </conditional>
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86 </section>
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87
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88 <!-- SNP Model options -->
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89 <section name="snp_options" title="SNP Model Options (ustacks options)" expanded="False">
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90 <expand macro="snp_options"/>
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91 </section>
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92 </inputs>
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93
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94 <outputs>
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95 <data format="txt" name="output_log" label="rxstacks.log with ${tool.name} on ${on_string}" from_work_dir="stacks_outputs/batch_1.rxstacks.log" />
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96
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97 <data format="txt" name="output_lnl_dist" label="Distribution of mean log likelihoods with ${tool.name} on ${on_string}" from_work_dir="stacks_outputs/batch_1.rxstacks_lnls.tsv">
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98 <filter>options_filtering['lnl_dist']</filter>
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99 </data>
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100
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101 <collection name="tags" type="list" label="Assembled loci filtered from ${on_string}">
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102 <discover_datasets pattern="(?P&lt;name&gt;.+\.tags)\.tsv$" ext="tabular" directory="stacks_outputs" />
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103 </collection>
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104
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105 <collection name="snps" type="list" label="Model calls from each locus filtered from ${on_string}">
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106 <discover_datasets pattern="(?P&lt;name&gt;.+\.snps)\.tsv$" ext="tabular" directory="stacks_outputs" />
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107 </collection>
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108
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109 <collection name="alleles" type="list" label="Haplotypes/alleles recorded from each locus filtered from ${on_string}">
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110 <discover_datasets pattern="(?P&lt;name&gt;.+\.alleles)\.tsv$" ext="tabular" directory="stacks_outputs" />
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111 </collection>
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112
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113 <collection name="all_output" type="list" label="Full output from rxstacks on ${on_string}">
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114 <discover_datasets pattern="(?P&lt;name&gt;.+\.(tags|snps|alleles))\.tsv$" ext="tabular" directory="stacks_outputs" />
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115 </collection>
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116 </outputs>
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117
caf3e2905cf3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks commit e1c1550e0bd61c88ffead2b1c4f6ab7393052393
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118 <tests>
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119 <test>
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120 <param name="input_col">
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121 <collection type="list">
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122 <element name="batch_1.catalog.alleles.tsv" ftype="tabular" value="genotypes/batch_1.catalog.alleles.tsv" />
caf3e2905cf3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks commit e1c1550e0bd61c88ffead2b1c4f6ab7393052393
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123 <element name="batch_1.catalog.snps.tsv" ftype="tabular" value="genotypes/batch_1.catalog.snps.tsv" />
caf3e2905cf3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks commit e1c1550e0bd61c88ffead2b1c4f6ab7393052393
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124 <element name="batch_1.catalog.tags.tsv" ftype="tabular" value="genotypes/batch_1.catalog.tags.tsv" />
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125 <element name="PopA_01.alleles.tsv" ftype="tabular" value="genotypes/PopA_01.alleles.tsv" />
caf3e2905cf3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks commit e1c1550e0bd61c88ffead2b1c4f6ab7393052393
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126 <element name="PopA_01.matches.tsv" ftype="tabular" value="genotypes/PopA_01.matches.tsv" />
caf3e2905cf3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks commit e1c1550e0bd61c88ffead2b1c4f6ab7393052393
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127 <element name="PopA_01.snps.tsv" ftype="tabular" value="genotypes/PopA_01.snps.tsv" />
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128 <element name="PopA_01.tags.tsv" ftype="tabular" value="genotypes/PopA_01.tags.tsv" />
caf3e2905cf3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks commit e1c1550e0bd61c88ffead2b1c4f6ab7393052393
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129 <element name="PopA_02.alleles.tsv" ftype="tabular" value="genotypes/PopA_02.alleles.tsv" />
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130 <element name="PopA_02.matches.tsv" ftype="tabular" value="genotypes/PopA_02.matches.tsv" />
caf3e2905cf3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks commit e1c1550e0bd61c88ffead2b1c4f6ab7393052393
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131 <element name="PopA_02.snps.tsv" ftype="tabular" value="genotypes/PopA_02.snps.tsv" />
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132 <element name="PopA_02.tags.tsv" ftype="tabular" value="genotypes/PopA_02.tags.tsv" />
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133 </collection>
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134 </param>
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135 <param name="options_filtering|lnl" value="-10.0" />
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136 <param name="options_filtering|lnl_dist" value="true" />
5
73dce6ae6b7f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks commit dc23703c260d004a28fe24a2a7c00cb4371bc32e
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137 <param name="options_filtering|prune_haplo" value="yes" />
0
caf3e2905cf3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks commit e1c1550e0bd61c88ffead2b1c4f6ab7393052393
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138 <param name="options_filtering|max_haplo" value="1" />
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139
caf3e2905cf3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks commit e1c1550e0bd61c88ffead2b1c4f6ab7393052393
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140 <output name="output_log">
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141 <assert_contents>
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142 <has_text text="rxstacks executed" />
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143 </assert_contents>
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144 </output>
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145
caf3e2905cf3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks commit e1c1550e0bd61c88ffead2b1c4f6ab7393052393
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146 <output name="output_lnl_dist">
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147 <assert_contents>
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148 <has_text text="Median" />
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149 </assert_contents>
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150 </output>
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151
caf3e2905cf3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks commit e1c1550e0bd61c88ffead2b1c4f6ab7393052393
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152 <!-- samples -->
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153 <output_collection name="tags">
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154 <element name="PopA_01.tags">
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155 <assert_contents>
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156 <has_text text="lane1_fakedata7_0" />
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157 </assert_contents>
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158 </element>
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159 </output_collection>
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160 <output_collection name="snps">
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161 <element name="PopA_01.snps">
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162 <assert_contents>
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163 <has_text text="24.950" />
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164 </assert_contents>
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165 </element>
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166 </output_collection>
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167 <output_collection name="alleles">
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168 <element name="PopA_01.alleles">
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169 <assert_contents>
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170 <has_text text="AC" />
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171 </assert_contents>
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172 </element>
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173 </output_collection>
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174 </test>
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175 </tests>
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176
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177 <help>
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178 <![CDATA[
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179 .. class:: infomark
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180
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181 **What it does**
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182
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183 This program will run each of the Stacks components: first, running ustacks on each of the samples specified, building loci and calling SNPs in each. Second, cstacks will be run to create a catalog of all loci that were marked as 'parents' or 'samples' on the command line, and finally, sstacks will be executed to match each sample against the catalog. A bit more detail on this process can be found in the FAQ. The denovo_map.pl program will also load the results of each stage of the analysis: individual loci, the catalog, and matches against the catalog into the database (although this can be disabled). After matching, the program will build a database index to speed up access (index_radtags.pl) and enable web-based filtering.
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184
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185 --------
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186
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187 **Input files**
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188
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189 Output from denovo_map or ref_map
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190
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191 **Output files**
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192
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193 - XXX.tags.tsv file:
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194
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195 See `Stacks output description <http://catchenlab.life.illinois.edu/stacks/manual/#files>`_
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196
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197 Notes: For the tags file, each stack will start in the file with a consensus sequence for the entire stack followed by the flags for that stack. Then, each individual read that was merged into that stack will follow. The next stack will start with another consensus sequence.
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198
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199
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200 - XXX.snps.tsv file:
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201
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202 See `Stacks output description <http://catchenlab.life.illinois.edu/stacks/manual/#files>`_
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203
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204 Notes: If a stack has two SNPs called within it, then there will be two lines in this file listing each one.
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205
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206
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207 - XXX.alleles.tsv file:
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208
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209 See `Stacks output description <http://catchenlab.life.illinois.edu/stacks/manual/#files>`_
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210
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211 - XXX.matches.tsv file:
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212
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213 See `Stacks output description <http://catchenlab.life.illinois.edu/stacks/manual/#files>`_
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214
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215 Notes: Each line in this file records a match between a catalog locus and a locus in an individual, for a particular haplotype. The Batch ID plus the Catalog ID together represent a unique locus in the entire population, while the Sample ID and the Stack ID together represent a unique locus in an individual sample.
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216
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217 @STACKS_INFOS@
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218 ]]>
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219 </help>
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220 <expand macro="citation" />
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221 </tool>