Mercurial > repos > iuc > stacks2_ustacks
comparison macros_process.xml @ 0:9eaeea3fb1bb draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
| author | iuc |
|---|---|
| date | Mon, 01 Jul 2019 10:49:03 -0400 |
| parents | |
| children | aa646fe5a0b4 |
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| -1:000000000000 | 0:9eaeea3fb1bb |
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| 1 <?xml version="1.0"?> | |
| 2 | |
| 3 <!-- macros and tokens for process_radtags and process_short_reads --> | |
| 4 | |
| 5 <macros> | |
| 6 | |
| 7 <token name="@PROCESS_IOOPTIONS@"><![CDATA[ | |
| 8 -p stacks_inputs/ | |
| 9 #if $input_type.input_type_select == "paired" | |
| 10 --paired | |
| 11 #end if | |
| 12 -i $inputype | |
| 13 -b '$barcode' | |
| 14 $input_type.barcode_encoding | |
| 15 #if str( $outype ) != "auto" | |
| 16 -y $outype | |
| 17 #end if | |
| 18 -o stacks_outputs | |
| 19 ]]></token> | |
| 20 <xml name="process_output_types"> | |
| 21 <param name="outype" argument="-y" type="select" label="Output format" > | |
| 22 <option value="auto" selected="True">Same as input</option> | |
| 23 <option value="fastq">fastq</option> | |
| 24 <option value="fasta">fasta</option> | |
| 25 <option value="gzfastq">gzipped fastq</option> | |
| 26 <option value="gzfasta">gzipped fasta</option> | |
| 27 </param> | |
| 28 </xml> | |
| 29 | |
| 30 <xml name="discover_faqgz_output_macro" token_pattern="" token_dir=""> | |
| 31 <expand macro="discover_faq_output_macro" pattern="@PATTERN@" dir="@DIR@"/> | |
| 32 <discover_datasets pattern="@PATTERN@\.fq\.gz$" ext="fastqsanger.gz" directory="@DIR@/" /> | |
| 33 <discover_datasets pattern="@PATTERN@\.fa\.gz$" ext="fasta.gz" directory="@DIR@/" /> | |
| 34 </xml> | |
| 35 <xml name="discover_faq_output_macro" token_pattern="" token_dir=""> | |
| 36 <discover_datasets pattern="@PATTERN@\.fq$" ext="fastqsanger" directory="@DIR@/" /> | |
| 37 <discover_datasets pattern="@PATTERN@\.fa$" ext="fasta" directory="@DIR@/" /> | |
| 38 </xml> | |
| 39 | |
| 40 <xml name="process_outputs"> | |
| 41 <collection name="demultiplexed" type="list" label="${tool.name} on ${on_string} Demultiplexed reads"> | |
| 42 <filter>input_type['input_type_select'] == "single"</filter> | |
| 43 <expand macro="discover_faqgz_output_macro" pattern="(?P<name>.+)" dir="stacks_outputs"/> | |
| 44 </collection> | |
| 45 <collection name="demultiplexed_paired" type="list:paired" label="${tool.name} on ${on_string} Demultiplexed reads"> | |
| 46 <filter>input_type['input_type_select'] == "paired"</filter> | |
| 47 <expand macro="discover_faqgz_output_macro" pattern="(?P<identifier_0>.+)\.(?P<identifier_1>[^.]+)" dir="stacks_outputs"/> | |
| 48 </collection> | |
| 49 | |
| 50 <collection name="remaining" type="list:paired" label="${tool.name} on ${on_string} Remaining orphan reads"> | |
| 51 <filter>input_type['input_type_select'] == "paired"</filter> | |
| 52 <expand macro="discover_faqgz_output_macro" pattern="(?P<identifier_0>.+)\.rem\.(?P<identifier_1>[^.]+)" dir="stacks_outputs/remaining"/> | |
| 53 </collection> | |
| 54 | |
| 55 <!-- note irrespective of -y output is always named fastq and are never zipped --> | |
| 56 <collection name="discarded" type="list" label="${tool.name} on ${on_string} Discarded reads"> | |
| 57 <filter>capture is True and input_type['input_type_select'] == "single"</filter> | |
| 58 <expand macro="discover_faq_output_macro" pattern="(?P<name>.*)" dir="stacks_outputs/discarded"/> | |
| 59 </collection> | |
| 60 <collection name="discarded_paired" type="list:paired" label="${tool.name} on ${on_string} Discarded reads"> | |
| 61 <filter>capture is True and input_type['input_type_select'] == "paired"</filter> | |
| 62 <expand macro="discover_faq_output_macro" pattern="(?P<identifier_0>.+)\.(?P<identifier_1>[^.]+)" dir="stacks_outputs/discarded"/> | |
| 63 </collection> | |
| 64 </xml> | |
| 65 | |
| 66 <!-- FASTQ filtering options --> | |
| 67 <xml name="process_filter"> | |
| 68 <conditional name="filter_cond" > | |
| 69 <param name="filter_select" type="select" label="Do quality filtering"> | |
| 70 <option value="yes">Yes</option> | |
| 71 <option value="no" selected="true">No</option> | |
| 72 </param> | |
| 73 <when value="yes"> | |
| 74 <param name="sliding" type="float" value="0.15" min="0" max="1" argument="-w" label="Set the size of the sliding window as a fraction of the read length, between 0 and 1" /> | |
| 75 <param name="score" type="integer" value="10" min="0" max="40" argument="-s" label="Set the score limit. If the average score within the sliding window drops below this value, the read is discarded" /> | |
| 76 <param name="remove" type="boolean" checked="false" truevalue="-c" falsevalue="" argument="-c" label="Clean data, remove any read with an uncalled base" /> | |
| 77 <param name="discard" type="boolean" checked="false" truevalue="-q" falsevalue="" argument="-q" label="Discard reads with low quality scores"/> | |
| 78 <param argument="--filter_illumina" type="boolean" checked="false" truevalue="--filter_illumina" falsevalue="" label="Discard reads that have been marked by Illumina's chastity/purity filter as failing" /> | |
| 79 </when> | |
| 80 <when value="no"> | |
| 81 <param argument="--len_limit" type="integer" value="" optional="true" label="Minimum sequence length" help="useful if your data has already been trimmed"/> | |
| 82 </when> | |
| 83 </conditional> | |
| 84 <param name="capture" type="boolean" checked="false" truevalue="-D" falsevalue="" argument="-D" label="Capture discarded reads to a file" /> | |
| 85 </xml> | |
| 86 <token name="@PROCESS_FILTER@"><![CDATA[ | |
| 87 #if $filter_cond.filter_select == 'yes': | |
| 88 -w $filter_cond.sliding | |
| 89 -s $filter_cond.score | |
| 90 $filter_cond.remove | |
| 91 $filter_cond.discard | |
| 92 $filter_cond.filter_illumina | |
| 93 #else | |
| 94 #if str($filter_cond.len_limit) != "": | |
| 95 --len_limit $filter_cond.len_limit | |
| 96 #end if | |
| 97 #end if | |
| 98 $capture | |
| 99 ]]></token> | |
| 100 <token name="@PROCESS_FASTQ_POSTPROC@"><![CDATA[ | |
| 101 #if $capture: | |
| 102 && mkdir stacks_outputs/discarded/ | |
| 103 && mv stacks_outputs/*discards stacks_outputs/discarded/ | |
| 104 | |
| 105 ## fix the _R[12]_0 that was added for preparing the input | |
| 106 #if $input_type.input_type_select == 'paired': | |
| 107 && find stacks_outputs/discarded/ -type f | while read file; do mv "\$file" "\$(echo \$file | sed 's/_R1_0/.1/; s/_R2_0/.2/;')"; done | |
| 108 #end if | |
| 109 ## also remove the gz which is added by procrad (but its uncompressed) | |
| 110 && find stacks_outputs/discarded/ -type f -iname "*.gz.discards" | while read file; do mv "\$file" "\$(echo \$file | sed 's/.gz.discards$/.discards/;')"; done | |
| 111 | |
| 112 ## the discard files are named fastq even if the output is fasta | |
| 113 #if str($outype).endswith("fasta"): | |
| 114 && find stacks_outputs/discarded/ -type f | while read file; do mv "\$file" "\$(echo \$file | sed 's/\.fastq.discards/.fa/;')"; done | |
| 115 #else | |
| 116 && find stacks_outputs/discarded/ -type f | while read file; do mv "\$file" "\$(echo \$file | sed 's/\.fastq.discards/.fq/;')"; done | |
| 117 #end if | |
| 118 #end if | |
| 119 ## prepare paired read output for processing in galaxy | |
| 120 #if $input_type.input_type_select == 'paired': | |
| 121 && mkdir stacks_outputs/remaining | |
| 122 && find stacks_outputs -iregex ".*\.rem\.[12]\.f[aq]\(\.gz\)?" | while read file; do mv "\$file" stacks_outputs/remaining/; done | |
| 123 && find stacks_outputs/ -iregex ".*.f[aq]\(\.gz\)?" | while read file; do mv "\$file" "\$(echo \$file | sed 's/\.1\./.forward./; s/\.2\./.reverse./')"; done | |
| 124 #end if | |
| 125 ]]></token> | |
| 126 | |
| 127 <!-- adapter trimming options --> | |
| 128 <xml name="process_adapter"> | |
| 129 <param argument="--adapter_1" type="text" value="" optional="true" label="Adaptor sequence that may occur on the first read" /> | |
| 130 <param argument="--adapter_2" type="text" value="" optional="true" label="Adaptor sequence that may occur on the paired-read" /> | |
| 131 <param argument="--adapter_mm" type="integer" value="" optional="true" label="Number of mismatches allowed in the adapter sequence"/> | |
| 132 </xml> | |
| 133 <token name="@PROCESS_ADAPTER@"><![CDATA[ | |
| 134 ## Adapter options | |
| 135 #if str($options_advanced.adapter_1) != "": | |
| 136 --adapter_1 $options_advanced.adapter_1 | |
| 137 #end if | |
| 138 #if str($options_advanced.adapter_2) != "": | |
| 139 --adapter_2 $options_advanced.adapter_2 | |
| 140 #end if | |
| 141 #if str($options_advanced.adapter_mm) != "": | |
| 142 --adapter_mm $options_advanced.adapter_mm | |
| 143 #end if | |
| 144 ]]></token> | |
| 145 | |
| 146 <!-- barcode rescue options --> | |
| 147 <xml name="rescue_barcode"> | |
| 148 <conditional name="rescue_cond"> | |
| 149 <param name="rescue" type="select" argument="-r" label="Rescue mutated barcodes and RAD-Tags?"> | |
| 150 <option value="-r">yes</option> | |
| 151 <option value="" selected="true">no</option> | |
| 152 </param> | |
| 153 <when value="-r"> | |
| 154 <param argument="--barcode_dist_1" type="integer" value="" optional="true" label="Number of allowed mismatches when rescuing first read barcodes" help="(default 1)"/> | |
| 155 <param argument="--barcode_dist_2" type="integer" value="" optional="true" label="Number of allowed mismatches when rescuing paired read barcodes" help="(default value for single end barcodes)"/> | |
| 156 </when> | |
| 157 <when value=""/> | |
| 158 </conditional> | |
| 159 </xml> | |
| 160 <token name="@RESCUE_BARCODE@"><![CDATA[ | |
| 161 #if str($options_advanced.rescue_cond.rescue) != "" | |
| 162 $options_advanced.rescue_cond.rescue | |
| 163 #if str($options_advanced.rescue_cond.barcode_dist_1) != "": | |
| 164 --barcode_dist_1 $options_advanced.rescue_cond.barcode_dist_1 | |
| 165 #end if | |
| 166 #if str($options_advanced.rescue_cond.barcode_dist_2) != "": | |
| 167 --barcode_dist_2 $options_advanced.rescue_cond.barcode_dist_2 | |
| 168 #end if | |
| 169 #end if | |
| 170 ]]></token> | |
| 171 | |
| 172 <!-- advanced options that are shared --> | |
| 173 <xml name="common_advanced"> | |
| 174 <param name="truncate" type="integer" value="" optional="True" argument="-t" label="Truncate final read length to this value" /> | |
| 175 <param argument="--retain_header" type="boolean" checked="false" truevalue="--retain_header" falsevalue="" label="Retain unmodified FASTQ headers in the output" /> | |
| 176 </xml> | |
| 177 <token name="@COMMON_ADVANCED@"><![CDATA[ | |
| 178 #if str($options_advanced.truncate) | |
| 179 -t $options_advanced.truncate | |
| 180 #end if | |
| 181 $options_advanced.retain_header | |
| 182 ]]></token> | |
| 183 </macros> |
