Mercurial > repos > iuc > sinto_barcode

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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sinto commit 4a156b17a386e1ecf13dfb2b232a1fc7d8344adc
author iuc
date Tue, 05 Nov 2024 11:50:43 +0000
parents f1d83e3a2357
children
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1 <tool id="sinto_barcode" name="Sinto barcode" profile="20.01" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@">
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2 <description>add cell barcodes to FASTQ read names</description>
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3 <macros>
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4 <import>macros.xml</import>
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5 </macros>
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6 <expand macro="bio_tools"/>
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7 <requirements>
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8 <requirement type="package" version="@TOOL_VERSION@">sinto</requirement>
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9 </requirements>
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10 <version_command>sinto --version</version_command>
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11 <command><![CDATA[
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12 ln -s '$barcode_fastq' barcodes.fastq.gz &&
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13 ln -s '${fastq_input.read1_fastq}' read1.fastq.gz &&
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14 #if str( $fastq_input.fastq_input_selector ) == "paired":
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15 ln -s '${fastq_input.read2_fastq}' read2.fastq.gz &&
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16 #end if
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17 sinto barcode
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18 --barcode_fastq barcodes.fastq.gz
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19 --read1 read1.fastq.gz
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20 #if str( $fastq_input.fastq_input_selector ) == "paired":
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21 --read2 read2.fastq.gz
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22 #end if
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23 --bases $bases
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24 ]]> </command>
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25 <inputs>
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26 <param type="data" name="barcode_fastq" format="fastqsanger.gz" label="FASTQ file containing cell barcode sequences" />
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27 <conditional name="fastq_input">
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28 <param name="fastq_input_selector" type="select" label="Single or Paired-end data">
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29 <option value="paired">Paired</option>
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30 <option value="single">Single</option>
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31 </param>
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32 <when value="paired">
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33 <param name="read1_fastq" type="data" format="fastqsanger.gz" label="Forward reads FASTQ file" />
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34 <param name="read2_fastq" type="data" format="fastqsanger.gz" label="Reverse reads FASTQ file" />
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35 </when>
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36 <when value="single">
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37 <param name="read1_fastq" type="data" format="fastqsanger.gz" label="Select FASTQ file" />
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38 </when>
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39 </conditional>
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40 <param type="integer" name="bases" value="16" min="0" label="Number of bases to extract from barcode-containing FASTQ" />
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41 </inputs>
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42 <outputs>
1
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43 <data name='read1_out' format='fastqsanger.gz' label="${tool.name} on ${on_string}: barcoded read 1" from_work_dir="read1.barcoded.fastq.gz" />
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44 <data name='read2_out' format='fastqsanger.gz' label="${tool.name} on ${on_string}: barcoded read 2" from_work_dir="read2.barcoded.fastq.gz" >
0
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45 <filter>fastq_input['fastq_input_selector'] == 'paired'</filter>
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46 </data>
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47 </outputs>
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48 <tests>
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49 <test expect_num_outputs="1">
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50 <param name="barcode_fastq" value="barcodes.fastq.gz" />
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51 <param name="fastq_input_selector" value="single"/>
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52 <param name="read1_fastq" value="read1.fastq.gz" />
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53 <output name="read1_out" file="read1.barcoded.fastq.gz" ftype="fastqsanger.gz" decompress="true" />
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54 </test>
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55 <test expect_num_outputs="2">
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56 <param name="barcode_fastq" value="barcodes.fastq.gz" />
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57 <param name="fastq_input_selector" value="paired"/>
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58 <param name="read1_fastq" value="read1.fastq.gz" />
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59 <param name="read2_fastq" value="read2.fastq.gz" />
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60 <output name="read1_out" file="read1.barcoded.fastq.gz" ftype="fastqsanger.gz" decompress="true"/>
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61 <output name="read2_out" file="read2.barcoded.fastq.gz" ftype="fastqsanger.gz" decompress="true"/>
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62 </test>
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63 </tests>
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64 <help><![CDATA[
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65
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66 Sinto is a toolkit for processing aligned single-cell data.
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67 --------------------------------------------------------------------------------------------------------------
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68 Cell barcodes from one FASTQ file added to the read names of another, or the same, FASTQ file. This is useful when processing raw single-cell sequencing data, as the cell barcode information can easily be propagated to the aligned BAM file by encoding the cell barcode in the read name.
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69
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70 **Inputs**
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71 FASTQ files containing barcodes and forward reads. An optional reverse reads FASTQ file can be provided for paired-end experiments. Note that all the FASTQs must contain the same number of reads and the reads must appear in the same order.
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72
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73 **Outputs**
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74 FASTQ files with the read names modified to contain the cell barcode sequence at the beginning of the read name, separated from the original read name by a : character.
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75
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76 ]]> </help>
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77 <expand macro="citations" />
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78 </tool>