Mercurial > repos > iuc > scanpy_filter
comparison filter.xml @ 3:1a4b233265e3 draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 3b41d687ff30583540d055f6995de00530cca81d"
| author | iuc |
|---|---|
| date | Thu, 12 Dec 2019 14:21:41 +0000 |
| parents | 21de91f71706 |
| children | 6d102b066d0c |
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| 2:21de91f71706 | 3:1a4b233265e3 |
|---|---|
| 111 </configfiles> | 111 </configfiles> |
| 112 <inputs> | 112 <inputs> |
| 113 <expand macro="inputs_anndata"/> | 113 <expand macro="inputs_anndata"/> |
| 114 <conditional name="method"> | 114 <conditional name="method"> |
| 115 <param argument="method" type="select" label="Method used for filtering"> | 115 <param argument="method" type="select" label="Method used for filtering"> |
| 116 <option value="pp.filter_cells">Filter cell outliers based on counts and numbers of genes expressed, using `pp.filter_cells`</option> | 116 <option value="pp.filter_cells">Filter cell outliers based on counts and numbers of genes expressed, using 'pp.filter_cells'</option> |
| 117 <option value="pp.filter_genes">Filter genes based on number of cells or counts, using `pp.filter_genes`</option> | 117 <option value="pp.filter_genes">Filter genes based on number of cells or counts, using 'pp.filter_genes'</option> |
| 118 <option value="tl.filter_rank_genes_groups">Filters out genes based on fold change and fraction of genes expressing the gene within and outside the groupby categories, using `tl.filter_rank_genes_groups`</option> | 118 <option value="tl.filter_rank_genes_groups">Filters out genes based on fold change and fraction of genes expressing the gene within and outside the groupby categories, using 'tl.filter_rank_genes_groups'</option> |
| 119 <option value="pp.highly_variable_genes">, using `tl.highly_variable_genes`</option> | 119 <option value="pp.highly_variable_genes">Annotate (and filter) highly variable genes, using 'pp.highly_variable_genes'</option> |
| 120 <option value="pp.subsample">Subsample to a fraction of the number of observations, using `pp.subsample`</option> | 120 <option value="pp.subsample">Subsample to a fraction of the number of observations, using 'pp.subsample'</option> |
| 121 <option value="pp.downsample_counts">Downsample counts from count matrix, using `pp.downsample_counts`</option> | 121 <option value="pp.downsample_counts">Downsample counts from count matrix, using 'pp.downsample_counts'</option> |
| 122 </param> | 122 </param> |
| 123 <when value="pp.filter_cells"> | 123 <when value="pp.filter_cells"> |
| 124 <conditional name="filter"> | 124 <conditional name="filter"> |
| 125 <param argument="filter" type="select" label="Filter"> | 125 <param argument="filter" type="select" label="Filter"> |
| 126 <option value="min_counts">Minimum number of counts</option> | 126 <option value="min_counts">Minimum number of counts</option> |
| 175 <param argument="min_fold_change" type="integer" value="2" label="Minimum fold change"/> | 175 <param argument="min_fold_change" type="integer" value="2" label="Minimum fold change"/> |
| 176 </when> | 176 </when> |
| 177 <when value="pp.highly_variable_genes"> | 177 <when value="pp.highly_variable_genes"> |
| 178 <conditional name='flavor'> | 178 <conditional name='flavor'> |
| 179 <param argument="flavor" type="select" label="Flavor for computing normalized dispersion"> | 179 <param argument="flavor" type="select" label="Flavor for computing normalized dispersion"> |
| 180 <option value="seurat">seurat: expects non-logarithmized data</option> | 180 <option value="seurat">Seurat</option> |
| 181 <option value="cell_ranger">cell_ranger: usually called for logarithmized data</option> | 181 <option value="cell_ranger">Cell Ranger</option> |
| 182 </param> | 182 </param> |
| 183 <when value="seurat"> | 183 <when value="seurat"> |
| 184 <param argument="min_mean" type="float" value="0.0125" label="Minimal mean cutoff"/> | 184 <param argument="min_mean" type="float" value="0.0125" label="Minimal mean cutoff"/> |
| 185 <param argument="max_mean" type="float" value="3" label="Maximal mean cutoff"/> | 185 <param argument="max_mean" type="float" value="3" label="Maximal mean cutoff"/> |
| 186 <param argument="min_disp" type="float" value="0.5" label="Minimal normalized dispersion cutoff"/> | 186 <param argument="min_disp" type="float" value="0.5" label="Minimal normalized dispersion cutoff"/> |
| 189 <when value="cell_ranger"> | 189 <when value="cell_ranger"> |
| 190 <param argument="n_top_genes" type="integer" value="" label="Number of highly-variable genes to keep"/> | 190 <param argument="n_top_genes" type="integer" value="" label="Number of highly-variable genes to keep"/> |
| 191 </when> | 191 </when> |
| 192 </conditional> | 192 </conditional> |
| 193 <param argument="n_bins" type="integer" value="20" label="Number of bins for binning the mean gene expression" help="Normalization is done with respect to each bin. If just a single gene falls into a bin, the normalized dispersion is artificially set to 1"/> | 193 <param argument="n_bins" type="integer" value="20" label="Number of bins for binning the mean gene expression" help="Normalization is done with respect to each bin. If just a single gene falls into a bin, the normalized dispersion is artificially set to 1"/> |
| 194 <param argument="subset" type="boolean" truevalue="True" falsevalue="False" checked="true" label="Inplace subset to highly-variable genes?" help="Otherwise it merely indicates highly variable genes."/> | 194 <param argument="subset" type="boolean" truevalue="True" falsevalue="False" checked="false" label="Inplace subset to highly-variable genes?" help="Otherwise it merely indicates highly variable genes."/> |
| 195 </when> | 195 </when> |
| 196 <when value="pp.subsample"> | 196 <when value="pp.subsample"> |
| 197 <conditional name="type"> | 197 <conditional name="type"> |
| 198 <param name="type" type="select" label="Type of subsampling"> | 198 <param name="type" type="select" label="Type of subsampling"> |
| 199 <option value="fraction">By fraction</option> | 199 <option value="fraction">By fraction</option> |
| 200 <option value="n_obs">By number of observation</option> | 200 <option value="n_obs">By number of observation</option> |
| 201 </param> | 201 </param> |
| 202 <when value="fraction"> | 202 <when value="fraction"> |
| 203 <param argument="fraction" type="float" value="" label="Subsample to this `fraction` of the number of observations"/> | 203 <param argument="fraction" type="float" value="" label="Subsample to this 'fraction' of the number of observations"/> |
| 204 </when> | 204 </when> |
| 205 <when value="n_obs"> | 205 <when value="n_obs"> |
| 206 <param argument="n_obs" type="integer" min="0" value="" label="Subsample to this number of observations"/> | 206 <param argument="n_obs" type="integer" min="0" value="" label="Subsample to this number of observations"/> |
| 207 </when> | 207 </when> |
| 208 </conditional> | 208 </conditional> |
| 327 <conditional name="flavor"> | 327 <conditional name="flavor"> |
| 328 <param name="flavor" value="cell_ranger"/> | 328 <param name="flavor" value="cell_ranger"/> |
| 329 <param name="n_top_genes" value="2"/> | 329 <param name="n_top_genes" value="2"/> |
| 330 </conditional> | 330 </conditional> |
| 331 <param name="n_bins" value="20"/> | 331 <param name="n_bins" value="20"/> |
| 332 <param name="subset" value="true"/> | |
| 332 </conditional> | 333 </conditional> |
| 333 <assert_stdout> | 334 <assert_stdout> |
| 334 <has_text_matching expression="sc.pp.highly_variable_genes"/> | 335 <has_text_matching expression="sc.pp.highly_variable_genes"/> |
| 335 <has_text_matching expression="flavor='cell_ranger'"/> | 336 <has_text_matching expression="flavor='cell_ranger'"/> |
| 336 <has_text_matching expression="n_top_genes=2"/> | 337 <has_text_matching expression="n_top_genes=2"/> |
| 337 <has_text_matching expression="n_bins=20"/> | 338 <has_text_matching expression="n_bins=20"/> |
| 339 <has_text_matching expression="subset=True"/> | |
| 338 </assert_stdout> | 340 </assert_stdout> |
| 339 <output name="anndata_out" file="pp.highly_variable_genes.krumsiek11-cell_ranger.h5ad" ftype="h5ad" compare="sim_size"/> | 341 <output name="anndata_out" file="pp.highly_variable_genes.krumsiek11-cell_ranger.h5ad" ftype="h5ad" compare="sim_size"/> |
| 340 </test> | 342 </test> |
| 341 <test> | 343 <test> |
| 342 <!-- test 7 --> | 344 <!-- test 7 --> |
