Mercurial > repos > iuc > samtools_view
comparison macros.xml @ 12:b2821e450815 draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools/samtools_view commit c5ded4208dd70e88596ddc725795a2401773f02d"
| author | iuc |
|---|---|
| date | Sat, 27 Nov 2021 12:30:31 +0000 |
| parents | 0b341e4e6ec1 |
| children | e63aab0f18c6 |
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| 11:61d97dda5ece | 12:b2821e450815 |
|---|---|
| 48 #end if | 48 #end if |
| 49 #end if | 49 #end if |
| 50 #end for | 50 #end for |
| 51 ]]></token> | 51 ]]></token> |
| 52 <token name="@PREPARE_FASTA_IDX@"><![CDATA[ | 52 <token name="@PREPARE_FASTA_IDX@"><![CDATA[ |
| 53 ##checks for reference data ($addref_cond.addref_select=="history" or =="cached") | 53 ## Make the user-selected reference genome, if any, accessible through |
| 54 ##and sets the -t/-T parameters accordingly: | 54 ## a shell variable $reffa, index the reference if necessary, and make |
| 55 ##- in case of history a symbolic link is used because samtools (view) will generate | 55 ## the fai-index file available through a shell variable $reffai. |
| 56 ## the index which might not be possible in the directory containing the fasta file | 56 |
| 57 ##- in case of cached the absolute path is used which allows to read the cram file | 57 ## For a cached genome simply sets the shell variables to point to the |
| 58 ## without specifying the reference | 58 ## genome file and its precalculated index. |
| 59 ## For a genome from the user's history, if that genome is a plain | |
| 60 ## fasta file, the code creates a symlink in the pwd, creates the fai | |
| 61 ## index file next to it, then sets the shell variables to point to the | |
| 62 ## symlink and its index. | |
| 63 ## For a fasta.gz dataset from the user's history, it tries the same, | |
| 64 ## but this will only succeed if the file got compressed with bgzip. | |
| 65 ## For a regular gzipped file samtools faidx will fail, in which case | |
| 66 ## the code falls back to decompressing to plain fasta before | |
| 67 ## reattempting the indexing. | |
| 68 ## Indexing of a bgzipped file produces a regular fai index file *and* | |
| 69 ## a compressed gzi file. The former is identical to the fai index of | |
| 70 ## the uncompressed fasta. | |
| 71 | |
| 72 ## If the user has not selected a reference (it's an optional parameter | |
| 73 ## in some samtools wrappers), a cheetah boolean use_ref is set to | |
| 74 ## False to encode that fact. | |
| 75 | |
| 76 #set use_ref=True | |
| 59 #if $addref_cond.addref_select == "history": | 77 #if $addref_cond.addref_select == "history": |
| 60 ln -s '${addref_cond.ref}' reference.fa && | 78 #if $addref_cond.ref.is_of_type('fasta'): |
| 61 samtools faidx reference.fa && | 79 reffa="reference.fa" && |
| 62 #set reffa="reference.fa" | 80 ln -s '${addref_cond.ref}' \$reffa && |
| 63 #set reffai="reference.fa.fai" | 81 samtools faidx \$reffa && |
| 82 #else: | |
| 83 reffa="reference.fa.gz" && | |
| 84 ln -s '${addref_cond.ref}' \$reffa && | |
| 85 { | |
| 86 samtools faidx \$reffa || | |
| 87 { | |
| 88 echo "Failed to index compressed reference. Trying decompressed ..." 1>&2 && | |
| 89 gzip -dc \$reffa > reference.fa && | |
| 90 reffa="reference.fa" && | |
| 91 samtools faidx \$reffa; | |
| 92 } | |
| 93 } && | |
| 94 #end if | |
| 95 reffai=\$reffa.fai && | |
| 64 #elif $addref_cond.addref_select == "cached": | 96 #elif $addref_cond.addref_select == "cached": |
| 65 #set reffa=str($addref_cond.ref.fields.path) | 97 ## in case of cached the absolute path is used which allows to read |
| 66 #set reffai=str($addref_cond.ref.fields.path)+".fai" | 98 ## a cram file without specifying the reference |
| 99 reffa='${addref_cond.ref.fields.path}' && | |
| 100 reffai=\$reffa.fai && | |
| 67 #else | 101 #else |
| 68 #set reffa=None | 102 #set use_ref=False |
| 69 #set reffai=None | |
| 70 #end if | 103 #end if |
| 71 ]]></token> | 104 ]]></token> |
| 72 | 105 |
| 73 <xml name="optional_reference"> | 106 <xml name="optional_reference"> |
| 74 <conditional name="addref_cond"> | 107 <conditional name="addref_cond"> |
