Mercurial > repos > iuc > mykrobe_genotype
comparison macro.xml @ 3:7a4c104238aa draft default tip
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mykrobe commit e0d4688a59e6eeba33adcfe803ac43d0bc2863e7"
| author | iuc |
|---|---|
| date | Wed, 01 Sep 2021 07:45:41 +0000 |
| parents | d779062514e0 |
| children |
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| 2:d779062514e0 | 3:7a4c104238aa |
|---|---|
| 1 <?xml version="1.1"?> | |
| 2 <macros> | 1 <macros> |
| 3 <xml name="requirements"> | 2 <token name="@TOOL_VERSION@">0.10.0</token> |
| 4 <requirements> | 3 <token name="@PROFILE@">20.09</token> |
| 5 <requirement type="package" version="0.7.0">mykrobe</requirement> | 4 <xml name="requirements"> |
| 6 </requirements> | 5 <requirements> |
| 7 </xml> | 6 <requirement type="package" version="@TOOL_VERSION@">mykrobe</requirement> |
| 8 <token name="@select_inputs@"> | 7 </requirements> |
| 9 <![CDATA[ | 8 </xml> |
| 10 #set $name='sample' | |
| 11 #set $type='fastq' | |
| 12 ## Adding sample name, indicate specie and inputs fastq(s) files | |
| 13 #if $data_type.type == "paired": | |
| 14 | 9 |
| 15 ln -s '$data_type.fastq_input1' sample_1.fastq && | 10 <token name="@shared_options@"> |
| 16 ln -s '$data_type.fastq_input2' sample_2.fastq && | 11 <![CDATA[ |
| 12 #if $kmer: | |
| 13 --kmer ${kmer} | |
| 14 #end if | |
| 15 #if $expected_depth: | |
| 16 --expected_depth ${expected_depth} | |
| 17 #end if | |
| 18 $ont | |
| 19 $report_all_calls | |
| 20 #if $expected_error_rate: | |
| 21 --expected_error_rate ${expected_error_rate} | |
| 22 #end if | |
| 23 #if $min_variant_conf: | |
| 24 --min_variant_conf ${min_variant_conf} | |
| 25 #end if | |
| 26 #if $min_gene_conf: | |
| 27 --min_gene_conf ${min_gene_conf} | |
| 28 #end if | |
| 29 #if $min_proportion_expected_depth: | |
| 30 --min_proportion_expected_depth ${min_proportion_expected_depth} | |
| 31 #end if | |
| 32 #if $min_gene_percent_covg_threshold: | |
| 33 --min_gene_percent_covg_threshold ${min_gene_percent_covg_threshold} | |
| 34 #end if | |
| 35 $guess_sequence_method | |
| 36 $ignore_minor_calls | |
| 37 #if $ignore_filtered: | |
| 38 --ignore_filtered '${ignore_filtered}' | |
| 39 #end if | |
| 40 #if $model: | |
| 41 --model '${model}' | |
| 42 #end if | |
| 43 #if $ploidy: | |
| 44 --ploidy '${ploidy}' | |
| 45 #end if | |
| 46 ]]> | |
| 47 </token> | |
| 17 | 48 |
| 49 <xml name="inputs"> | |
| 50 <conditional name="data_type"> | |
| 51 <param name="type" type="select" label="Specify the read type."> | |
| 52 <option value="single">Single-end Data</option> | |
| 53 <option value="paired">Paired-end Data</option> | |
| 54 <option value="collection">Collection Paired-end Data</option> | |
| 55 </param> | |
| 56 <when value="single"> | |
| 57 <param name="seq1" type="data" format="fastqsanger,fastqsanger.gz,fastq,fastq.gz,fasta,fasta.gz,bam" label="Single end read file(s)"/> | |
| 58 </when> | |
| 59 <when value="paired"> | |
| 60 <param name="seq1" type="data" format="fastqsanger,fastqsanger.gz,fastq,fastq.gz" label="Forward paired-end read file"/> | |
| 61 <param name="seq2" type="data" format="fastqsanger,fastqsanger.gz,fastq,fastq.gz" label="Reverse paired-end read file"/> | |
| 62 </when> | |
| 63 <when value="collection"> | |
| 64 <param name="collection1" type="data_collection" label="Paired-end reads collection" optional="false" format="fastqsanger,fastqsanger.gz,fastq,fastq.gz" collection_type="paired" /> | |
| 65 </when> | |
| 66 </conditional> | |
| 67 </xml> | |
| 18 | 68 |
| 19 #elif $data_type.type == "collection": | 69 <xml name="options"> |
| 20 #set $name=str($data_type.fastq_input1.name) | 70 <param argument="--kmer" optional="True" type="integer" min="0" label="Kmer length" value="21" help="default = 21"/> |
| 21 ln -s '$data_type.fastq_input1.forward' '$data_type.fastq_input1.name'_1.fastq && | 71 <param argument="--expected_error_rate" optional="True" type="float" label="Expected error rate" help="Expected sequencing error rate. Set to 0.15 for ONT genotyping"/> |
| 22 ln -s '$data_type.fastq_input1.reverse' '$data_type.fastq_input1.name'_2.fastq && | 72 <param argument="--expected_depth" optional="True" type="integer" min="0" label="Expected depth" help=""/> |
| 73 <param argument="--ont" type="boolean" truevalue="--ont" falsevalue="" label="Set default for ONT data" help=""/> | |
| 74 <param argument="--min_variant_conf" optional="True" type="integer" min="0" label="Minimum genotype confidence for variant genotyping" help=""/> | |
| 75 <param argument="--min_gene_conf" optional="True" type="integer" min="0" label="Minimum genotype confidence for gene genotyping" help=""/> | |
| 76 <param argument="--min_gene_percent_covg_threshold" optional="True" type="integer" min="0" label="Minimum gene predict coverage" help="all genes alleles found above this percent coverage will be reported (default 100 (only best alleles reported))"/> | |
| 77 <param argument="--min_proportion_expected_depth" optional="True" type="float" label="Minimum depth required on the sum of both alleles" help="Default 0.3 (30%)"/> | |
| 78 <param argument="--model" optional="True" type="data" format="txt" label="Genotype model used" help="default kmer_count. Options kmer_count, median_depth"/> | |
| 79 <param argument="--ploidy" optional="True" type="select" label="Diploid or Haploid" help="Use a diploid (includes 0/1 calls) or haploid genotyping model"> | |
| 80 <option value="diploid">diploid</option> | |
| 81 <option value="haploid">haploid</option> | |
| 82 </param> | |
| 83 <param argument="--report_all_calls" type="boolean" truevalue="--report_all_calls" falsevalue="" label="Report all calls" help=""/> | |
| 84 <param argument="--ignore_minor_calls" type="boolean" truevalue="--ignore_minor_calls" falsevalue="" label="Ignore minor calls" help="Ignore minor calls when running resistance prediction"/> | |
| 85 <param argument="--ignore_filtered" type="data" format="txt" label="Ignore filtered" optional="True" help="Don't include filtered genotypes"/> | |
| 86 <param argument="--guess_sequence_method" type="boolean" truevalue="--guess_sequence_method" falsevalue="" label="Guess sequence method" help="Guess if ONT or Illumia based on error rate. If error rate is > 10%, ploidy is set to haploid and a confidence threshold is used"/> | |
| 87 </xml> | |
| 23 | 88 |
| 24 #elif $data_type.type == "single": | 89 <token name="@ATTRIBUTION@"> |
| 90 <![CDATA[ | |
| 91 **MyKrobe predict - Antibiotic resistance predictions** | |
| 92 Rapid antibiotic-resistance predictions from genome sequence data for Staphylococcus aureus and Mycobacterium tuberculosis | |
| 93 using Bruijn graph. | |
| 94 ]]> | |
| 95 </token> | |
| 25 | 96 |
| 26 | 97 <xml name="citation"> |
| 27 #if $data_type.fastq_input1.is_of_type('fastqsanger') or $data_type.fastq_input1.is_of_type('fastq'): | 98 <citations> |
| 28 ln -s '$data_type.fastq_input1' sample.fastq && | 99 <citation type="doi">10.1038/ncomms10063</citation> |
| 29 | 100 </citations> |
| 30 #end if | 101 </xml> |
| 31 | |
| 32 #if $data_type.fastq_input1.is_of_type('bam'): | |
| 33 ln -s '$data_type.fastq_input1' sample.bam && | |
| 34 #set $type='bam' | |
| 35 #end if | |
| 36 | |
| 37 #end if | |
| 38 ]]> | |
| 39 </token> | |
| 40 | |
| 41 <token name="@shared_options@"> | |
| 42 <![CDATA[ | |
| 43 #if $kmer: | |
| 44 --kmer '${kmer}' | |
| 45 #end if | |
| 46 #if $expected_depth: | |
| 47 --expected_depth '${expected_depth}' | |
| 48 #end if | |
| 49 | |
| 50 $ont | |
| 51 | |
| 52 $report_all_calls | |
| 53 | |
| 54 #if $expected_error_rate: | |
| 55 --expected_error_rate '${expected_error_rate}' | |
| 56 #end if | |
| 57 | |
| 58 #if $min_variant_conf: | |
| 59 --min_variant_conf '${min_variant_conf}' | |
| 60 #end if | |
| 61 | |
| 62 #if $min_gene_conf: | |
| 63 --min_gene_conf '${min_gene_conf}' | |
| 64 #end if | |
| 65 | |
| 66 #if $min_proportion_expected_depth: | |
| 67 --min_proportion_expected_depth '${min_proportion_expected_depth}' | |
| 68 #end if | |
| 69 | |
| 70 #if $min_gene_percent_covg_threshold: | |
| 71 --min_gene_percent_covg_threshold '${min_gene_percent_covg_threshold}' | |
| 72 #end if | |
| 73 | |
| 74 $guess_sequence_method | |
| 75 | |
| 76 $ignore_minor_calls | |
| 77 | |
| 78 #if $ignore_filtered: | |
| 79 --ignore_filtered '${ignore_filtered}' | |
| 80 #end if | |
| 81 | |
| 82 #if $model: | |
| 83 --model '${model}' | |
| 84 #end if | |
| 85 | |
| 86 #if $ploidy: | |
| 87 --ploidy '${ploidy}' | |
| 88 #end if | |
| 89 ]]> | |
| 90 </token> | |
| 91 | |
| 92 <xml name="inputs"> | |
| 93 <conditional name="data_type"> | |
| 94 <param name="type" type="select" label="Specify the read type."> | |
| 95 <option value="single">Single-end Data</option> | |
| 96 <option value="paired">Paired-end Data</option> | |
| 97 <option value="collection">Collection Paired-end Data</option> | |
| 98 </param> | |
| 99 <when value="single"> | |
| 100 <param name="fastq_input1" type="data" format="fastqsanger, fastq,fasta,bam" label="Single end read file(s)"/> | |
| 101 </when> | |
| 102 <when value="paired"> | |
| 103 <param name="fastq_input1" type="data" format="fastqsanger, fastq" label="Forward paired-end read file"/> | |
| 104 <param name="fastq_input2" type="data" format="fastqsanger, fastq" label="Reverse paired-end read file"/> | |
| 105 </when> | |
| 106 <when value="collection"> | |
| 107 <param name="fastq_input1" type="data_collection" label="Paired-end reads collection" optional="false" format="fastqsanger, fastq" collection_type="paired" /> | |
| 108 </when> | |
| 109 </conditional> | |
| 110 </xml> | |
| 111 | |
| 112 <xml name="options"> | |
| 113 <param argument="--kmer" optional="True" type="integer" min="0" label="Kmer length" value="21" help="default = 21"/> | |
| 114 <param argument="--expected_error_rate" optional="True" type="float" label="Expected error rate" help="Expected sequencing error rate. Set to 0.15 for ONT genotyping"/> | |
| 115 <param argument="--expected_depth" optional="True" type="integer" min="0" label="Expected depth" help=""/> | |
| 116 <param argument="--ont" type="boolean" truevalue="--ont" falsevalue="" label="Set default for ONT data" help=""/> | |
| 117 <param argument="--min_variant_conf" optional="True" type="integer" min="0" label="Minimum genotype confidence for variant genotyping" help=""/> | |
| 118 <param argument="--min_gene_conf" optional="True" type="integer" min="0" label="Minimum genotype confidence for gene genotyping" help=""/> | |
| 119 <param argument="--min_gene_percent_covg_threshold" optional="True" type="integer" min="0" label="Minimum gene predict coverage" help="all genes alleles found above this percent coverage will be reported (default 100 (only best alleles reported))"/> | |
| 120 <param argument="--min_proportion_expected_depth" optional="True" type="float" label="Minimum depth required on the sum of both alleles" help="Default 0.3 (30%)"/> | |
| 121 <param argument="--model" optional="True" type="data" format="txt" label="Genotype model used" help="default kmer_count. Options kmer_count, median_depth"/> | |
| 122 <param argument="--ploidy" optional="True" type="select" label="Diploid or Haploid" help="Use a diploid (includes 0/1 calls) or haploid genotyping model"> | |
| 123 <option value="diploid">diploid</option> | |
| 124 <option value="haploid">haploid</option> | |
| 125 </param> | |
| 126 <param argument="--report_all_calls" type="boolean" truevalue="--report_all_calls" falsevalue="" label="Report all calls" help=""/> | |
| 127 <param argument="--ignore_minor_calls" type="boolean" truevalue="--ignore_minor_calls" falsevalue="" label="Ignore minor calls" help="Ignore minor calls when running resistance prediction"/> | |
| 128 <param argument="--ignore_filtered" type="data" format="txt" label="Ignore filtered" optional="True" help="Don't include filtered genotypes"/> | |
| 129 <param argument="--guess_sequence_method" type="boolean" truevalue="--guess_sequence_method" falsevalue="" label="Guess sequence method" help="Guess if ONT or Illumia based on error rate. If error rate is > 10%, ploidy is set to haploid and a confidence threshold is used"/> | |
| 130 </xml> | |
| 131 | |
| 132 <token name="@ATTRIBUTION@"> | |
| 133 <![CDATA[ | |
| 134 | |
| 135 **MyKrobe predict - Antibiotic resistance predictions** | |
| 136 | |
| 137 Rapid antibiotic-resistance predictions from genome sequence data for Staphylococcus aureus and Mycobacterium tuberculosis | |
| 138 using Bruijn graph. | |
| 139 ]]> | |
| 140 </token> | |
| 141 | |
| 142 <xml name="citation"> | |
| 143 <citations> | |
| 144 <citation type="doi">10.1038/ncomms10063</citation> | |
| 145 </citations> | |
| 146 </xml> | |
| 147 </macros> | 102 </macros> |
