Mercurial > repos > iuc > mothur_make_contigs
diff make.contigs.xml @ 1:2de7963eb3ba draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit ea40e3d84e7850eb4226d6c85f709dcad18d4ba9
| author | iuc |
|---|---|
| date | Thu, 18 May 2017 18:19:45 -0400 |
| parents | d9268e6b2ea0 |
| children | 4cf175b57289 |
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--- a/make.contigs.xml Fri Jun 24 16:37:19 2016 -0400 +++ b/make.contigs.xml Thu May 18 18:19:45 2017 -0400 @@ -4,20 +4,37 @@ <import>macros.xml</import> </macros> <expand macro="requirements"/> + <expand macro="stdio"/> <expand macro="version_command"/> - <command detect_errors="aggressive"><![CDATA[ - ## create symlinks to input datasets - ln -s "$ffastq" ffastq.dat && - ln -s "$rfastq" rfastq.dat && + <command><![CDATA[ + @SHELL_OPTIONS@ + + ## Symlinks creation or On the fly creation of a combo file + #if $input_type.type == 'list_collection' + #for $pair in $input_type.list_paired_collection: + echo -e "${pair.name}\t${pair.forward}\t${pair.reverse}" >> combo_fastq.dat && + #end for + #elif $input_type.type == 'simple_collection' + ln -s '$input_type.paired_collection.forward' ffastq.dat && + ln -s '$input_type.paired_collection.reverse' rfastq.dat && + #else + ln -s '$input_type.forward_fastq' ffastq.dat && + ln -s '$input_type.reverse_fastq' rfastq.dat && + #end if + #if $oligo.add == "yes": - ln -s "$oligo.oligos" oligo.oligos.dat && - ln -s "$oligo.findex" oligo.findex.dat && - ln -s "$oligo.rindex" oligo.rindex.dat && + ln -s '$oligo.oligos' oligo.oligos.dat && + ln -s '$oligo.findex' oligo.findex.dat && + ln -s '$oligo.rindex' oligo.rindex.dat && #end if echo 'make.contigs( - ffastq=ffastq.dat, - rfastq=rfastq.dat, + #if $input_type.type == 'list_collection': + file=combo_fastq.dat, + #else: + ffastq=ffastq.dat, + rfastq=rfastq.dat, + #end if align=$align, #if $oligo.add == "yes": oligos=oligo.oligos.dat, @@ -39,10 +56,26 @@ )' | sed 's/ //g' ## mothur trips over whitespace | mothur + | tee mothur.out.log ]]></command> <inputs> - <param name="ffastq" type="data" format="fastq" label="ffastq - Forward Fastq Sequence file"/> - <param name="rfastq" type="data" format="fastq" label="rfastq - Reverse Fastq Sequence file"/> + <conditional name="input_type"> + <param name="type" type="select" label="Select a way to provide forward and reverse fastq files ?" help=""> + <option value="regular" selected="true">Two simple fastq files (forward and reverse)</option> + <option value="simple_collection">One pair (paired collection)</option> + <option value="list_collection">Multiple pairs - Combo mode (list:paired collection)</option> + </param> + <when value="regular"> + <param name="forward_fastq" type="data" format="fastq" label="Forward reads"/> + <param name="reverse_fastq" type="data" format="fastq" label="Reverse reads"/> + </when> + <when value="simple_collection"> + <param name="paired_collection" type="data_collection" format="fastq" collection_type="paired" label="Fastq pair (collection)" help="Dataset collection made from a single pair of fastq files (forward + reverse)"/> + </when> + <when value="list_collection"> + <param name="list_paired_collection" type="data_collection" format="fastq" collection_type="list:paired" label="Fastq pairs (collection)" help="Dataset collection made from multiple pairs of fastq files" /> + </when> + </conditional> <param name="align" type="select" label="align - Select a pairwise alignment method" help=""> <option value="needleman" selected="true">needleman (default) </option> <option value="gotoh">gotoh</option> @@ -55,7 +88,8 @@ </param> <when value="no"/> <when value="yes"> - <param name="oligos" type="data" format="mothur.oligos" optional="true" label="oligos - barcodes and primers" help="a file that can contain the sequences of the forward and reverse primers and barcodes and their sample identifier. Each line of the oligos file can start with the key words "forward", "reverse" and "barcode" or it can start with a "#" to tell mothur to ignore that line of the oligos file"/> + <param name="oligos" type="data" format="mothur.oligos" optional="true" label="oligos - barcodes and primers" + help="a file that can contain the sequences of the forward and reverse primers and barcodes and their sample identifier. Each line of the oligos file can start with the key words "forward", "reverse" and "barcode" or it can start with a "#" to tell mothur to ignore that line of the oligos file"/> <param name="bdiffs" type="integer" value="0" min="0" label="bdiffs - number of differences to allow in the barcode (default 0)"/> <param name="pdiffs" type="integer" value="0" min="0" label="pdiffs - number of differences to allow in the primer (default 0)"/> <param name="tdiffs" type="integer" value="0" min="0" label="tdiffs - total number of differences to allow in primer and barcode (default 0)"/> @@ -70,24 +104,55 @@ </inputs> <outputs> <expand macro="logfile-output"/> - <data name="fasta" format="fasta" from_work_dir="ffastq*.trim.*.fasta" label="${tool.name} on ${on_string}: trim.contigs.fasta"/> - <data name="qual" format="qual" from_work_dir="ffastq*.trim.*.qual" label="${tool.name} on ${on_string}: trim.contigs.qual"/> - <data name="scrapfasta" format="fasta" from_work_dir="ffastq*.scrap.*.fasta" label="${tool.name} on ${on_string}: scrap.contigs.fasta"/> - <data name="scrapqual" format="qual" from_work_dir="ffastq*.scrap.*.qual" label="${tool.name} on ${on_string}: scrap.contigs.qual"/> - <data name="report" format="txt" from_work_dir="ffastq*.contigs.report" label="${tool.name} on ${on_string}: report"/> + <data name="fasta" format="fasta" from_work_dir="*fastq.trim.*.fasta" label="${tool.name} on ${on_string}: trim.contigs.fasta"/> + <data name="qual" format="qual" from_work_dir="*fastq*.trim.*.qual" label="${tool.name} on ${on_string}: trim.contigs.qual"/> + <data name="scrapfasta" format="fasta" from_work_dir="*fastq*.scrap.*.fasta" label="${tool.name} on ${on_string}: scrap.contigs.fasta"/> + <data name="scrapqual" format="qual" from_work_dir="*fastq*.scrap.*.qual" label="${tool.name} on ${on_string}: scrap.contigs.qual"/> + <data name="report" format="txt" from_work_dir="*fastq*.contigs.report" label="${tool.name} on ${on_string}: report"/> + <data name="group" format="mothur.groups" from_work_dir="*fastq*.groups" label="${tool.name} on ${on_string}: group file"> + <filter>input_type['type'] == 'list_collection'</filter> + </data> </outputs> <tests> + <!-- Test with two regular files as input --> <test> - <param name="ffastq" value="Mock_S280_L001_R1_001_small.fastq"/> - <param name="rfastq" value="Mock_S280_L001_R2_001_small.fastq"/> + <conditional name="input_type"> + <param name="type" value="regular"/> + <param name="forward_fastq" value="Mock_S280_L001_R1_001_small.fastq" ftype="fastq"/> + <param name="reverse_fastq" value="Mock_S280_L001_R2_001_small.fastq" ftype="fastq"/> + </conditional> <output name="fasta" file="Mock_S280_L001_R1_001_small.trim.contigs.fasta" ftype="fasta"/> <output name="qual" file="Mock_S280_L001_R1_001_small.trim.contigs.qual" ftype="qual"/> <output name="report" file="Mock_S280_L001_R1_001_small.contigs.report" ftype="txt"/> <expand macro="logfile-test"/> </test> + <!-- Test with a simple paired collection as input --> <test> - <param name="ffastq" value="Mock_S280_L001_R1_001_small.fastq"/> - <param name="rfastq" value="Mock_S280_L001_R2_001_small.fastq"/> + <conditional name="input_type"> + <param name="type" value="simple_collection"/> + <param name="paired_collection"> + <collection type="paired"> + <element name="forward" value="Mock_S280_L001_R1_001_small.fastq" /> + <element name="reverse" value="Mock_S280_L001_R2_001_small.fastq" /> + </collection> + </param> + </conditional> + <output name="fasta" file="Mock_S280_L001_R1_001_small.trim.contigs.fasta" ftype="fasta"/> + <output name="qual" file="Mock_S280_L001_R1_001_small.trim.contigs.qual" ftype="qual"/> + <output name="report" file="Mock_S280_L001_R1_001_small.contigs.report" ftype="txt"/> + <expand macro="logfile-test"/> + </test> + <!-- Test with a simple paired collection as input + extra parameters specified --> + <test> + <conditional name="input_type"> + <param name="type" value="simple_collection"/> + <param name="paired_collection"> + <collection type="paired"> + <element name="forward" value="Mock_S280_L001_R1_001_small.fastq" /> + <element name="reverse" value="Mock_S280_L001_R2_001_small.fastq" /> + </collection> + </param> + </conditional> <param name="align" value="gotoh"/> <param name="match" value="2"/> <param name="mismatch" value="-2"/> @@ -98,17 +163,44 @@ <output name="report" md5="5274725ef45890fd6da4650d5d536173" ftype="txt"/> <expand macro="logfile-test"/> </test> + <!-- Test with a list:paired collection as input --> + <test> + <conditional name="input_type"> + <param name="type" value="list_collection"/> + <param name="list_paired_collection"> + <collection type="list:paired"> + <element name="Pair1"> + <collection type="paired"> + <element name="forward" value="Mock_S280_L001_R1_001_small.fastq" /> + <element name="reverse" value="Mock_S280_L001_R2_001_small.fastq" /> + </collection> + </element> + <element name="Pair2"> + <collection type="paired"> + <element name="forward" value="Mock_S280_L001_R1_001_small.fastq" /> + <element name="reverse" value="Mock_S280_L001_R2_001_small.fastq" /> + </collection> + </element> + </collection> + </param> + </conditional> + <output name="fasta" md5="dab69a0e36f718b55d8defad26ec469b" ftype="fasta"/> + <output name="qual" md5="cdba5409e4f87e3cd093a4e51084d616" ftype="qual"/> + <output name="report" md5="80b992abd7e4d6a5e89fa70011ef2384" ftype="txt"/> + <output name="group" md5="2be2adb030c4fc001bfac7e1172b40e1" ftype="mothur.groups"/> + <expand macro="logfile-test"/> + </test> </tests> <help> <![CDATA[ @MOTHUR_OVERVIEW@ -**Command Documenation** +**Command Documentation** The make.contigs_ command reads a forward fastq file and a reverse fastq file and outputs new fasta and quality files. -.. _make.contigs: http://www.mothur.org/wiki/Make.contigs +.. _make.contigs: https://www.mothur.org/wiki/Make.contigs v.1.27.0: Updated to use Mothur 1.33. Added findex and rindex parmaeters, optionally used with the oligos file. ]]>
