Mercurial > repos > iuc > mothur_get_oturep
diff get.oturep.xml @ 0:d568050a95be draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
author | iuc |
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date | Fri, 24 Jun 2016 16:33:26 -0400 |
parents | |
children | 17b63c3cc04d |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/get.oturep.xml Fri Jun 24 16:33:26 2016 -0400 @@ -0,0 +1,200 @@ +<tool profile="16.07" id="mothur_get_oturep" name="Get.oturep" version="@WRAPPER_VERSION@.0"> + <description>Generate a fasta with a representative sequence for each OTU</description> + <macros> + <import>macros.xml</import> + </macros> + <expand macro="requirements"/> + <expand macro="version_command"/> + <command detect_errors="aggressive"><![CDATA[ + ## create symlinks to input datasets + ln -s "$otu" otu.dat && + ln -s "$fasta" fasta.dat && + ln -s "$input.dist" input.dist.dat && + ln -s "$input.name" input.name.dat && + ln -s "$count" count.dat && + #if $pick.type == "yes": + ln -s "$pick.group" pick.group.dat && + #end if + + echo 'get.oturep( + list=otu.dat, + #if $fasta: + fasta=fasta.dat, + #end if + #if $input.source == "column": + column=input.dist.dat, + name=input.name.dat, + #elif $input.source == "phylip": + phylip=input.dist.dat, + #if $input.name: + name=input.name.dat, + #end if + #end if + #if $label: + label=${ str($label).replace(",","-") }, + #end if + #if $pick.type == "yes": + #if $pick.group: + group=pick.group.dat, + #end if + #if $pick.groups: + groups=${ str($pick.groups).replace(",","-") }, + #end if + #end if + #if $sorted: + sorted=$sorted, + #end if + #if $count and not $input.name: + count=count.dat, + #end if + method=$method, + large=$large + )' + | sed 's/ //g' ## mothur trips over whitespace + | mothur + ]]></command> + <inputs> + <param name="otu" type="data" format="mothur.list" label="list - OTU List"/> + <conditional name="input"> + <param name="source" type="select" label="Distance Matrix Format"> + <option value="column">Pairwise Column Distance Matrix</option> + <option value="phylip">Phylip Distance Matrix</option> + </param> + <when value="column"> + <param name="dist" type="data" format="mothur.pair.dist" label="column - Distance Matrix"/> + <param name="name" type="data" format="mothur.names" label="name - Sequences Name reference"/> + </when> + <when value="phylip"> + <param name="dist" type="data" format="mothur.lower.dist,mothur.square.dist" label="phylip - Distance Matrix"/> + <param name="name" type="data" format="mothur.names" optional="true" label="name - Names"/> + </when> + </conditional> + <param name="fasta" type="data" format="fasta" optional="true" label="fasta - Fasta"/> + <conditional name="pick"> + <param name="type" type="select" label="Parse a group file into abundant and rare groups?" help=""> + <option value="no" selected="true">No</option> + <option value="yes">Yes</option> + </param> + <when value="no"/> + <when value="yes"> + <param name="group" type="data" format="mothur.groups" label="group - Group file for the OTU List"/> + <param name="groups" type="select" multiple="true" label="groups - Group Selection (all used if none are selected)"> + <options> + <filter type="data_meta" ref="group" key="groups"/> + </options> + </param> + </when> + </conditional> + <param name="label" type="select" multiple="true" label="label - OTU Labels"> + <expand macro="labeloptions"/> + </param> + <param name="sorted" type="select" label="sorted - Sort Sequences by"> + <option value="">Don't sort</option> + <option value="name">Sequence Name</option> + <option value="number">Bin Number</option> + <option value="size">Bin Size</option> + <option value="group">Group</option> + </param> + <param name="large" type="boolean" checked="false" truevalue="true" falsevalue="false" label="large - Distance Matrix is very Large"/> + <param name="count" type="data" format="mothur.count_table" optional="true" label="count - a count table" help="Cannot supply both a names and count table. Will be ignored if you also supplied a name file. Generated by count.seqs"/> + <param name="method" type="select" label="method - select the method of selecting the representative sequence" help="the distance method finds the sequence with the smallest maximum distance to other sequences. The abundance method chooses the abundant sequence in the OTU as the representative. Default=distance"> + <option value="distance" selected="true">distance</option> + <option value="abundance">abundance</option> + </param> + </inputs> + <outputs> + <expand macro="logfile-output"/> + <collection name="names_out" type="list" label="${tool.name} on ${on_string}: rep.names"> + <filter>input['name'] or not count</filter> + <discover_datasets pattern=".*?\.(?P<designation>.*)\.rep\.names" format="mothur.names"/> + </collection> + <collection name="fasta_out" type="list" label="${tool.name} on ${on_string}: rep.fasta"> + <filter>fasta</filter> + <discover_datasets pattern=".*?\.(?P<designation>.*)\.rep\.fasta" format="fasta"/> + </collection> + <collection name="count_out" type="list" label="${tool.name} on ${on_string}: rep.count_table"> + <filter>count and not input['name']</filter> + <discover_datasets pattern=".*?\.(?P<designation>.*)\.rep\.count_table" format="mothur.count_table"/> + </collection> + </outputs> + <tests> + <test><!-- test with phylip --> + <param name="otu" value="amazon.an.list" ftype="mothur.list"/> + <param name="source" value="phylip"/> + <param name="dist" value="98_sq_phylip_amazon.dist" ftype="mothur.square.dist"/> + <param name="name" value="amazon1.names" ftype="mothur.names"/> + <output_collection name="names_out" count="36"> + <element name="0.27" md5="39ff2858909d49633871d5a625585de5" ftype="mothur.names"/> + </output_collection> + <expand macro="logfile-test"/> + </test> + <test><!-- test with column and label select --> + <param name="otu" value="amazon.an.list" ftype="mothur.list"/> + <param name="source" value="column"/> + <param name="dist" value="amazon.pair.dist" ftype="mothur.pair.dist"/> + <param name="name" value="amazon1.names" ftype="mothur.names"/> + <param name="label" value="unique,0.05,0.27,0.45"/> + <output_collection name="names_out" count="4"> + <element name="0.27" md5="be6dccdd3d4619c3ac465246c826209a" ftype="mothur.names"/> + </output_collection> + <expand macro="logfile-test"/> + </test> + <test><!-- test with fasta and sorting--> + <param name="otu" value="amazon.an.list" ftype="mothur.list"/> + <param name="source" value="phylip"/> + <param name="dist" value="98_sq_phylip_amazon.dist" ftype="mothur.square.dist"/> + <param name="fasta" value="amazon.fasta" ftype="fasta"/> + <param name="label" value="0.05,0.27"/> + <param name="sorted" value="name"/> + <output_collection name="names_out" count="2"> + <element name="0.27" md5="39ff2858909d49633871d5a625585de5" ftype="mothur.names"/> + </output_collection> + <output_collection name="fasta_out" count="2"> + <element name="0.27" md5="a9d9622b6f5fcbe8acdc05df5484ffc4" ftype="fasta"/> + </output_collection> + <expand macro="logfile-test"/> + </test> + <test><!-- test with group file --> + <param name="otu" value="amazon.an.list" ftype="mothur.list"/> + <param name="source" value="column"/> + <param name="dist" value="amazon.pair.dist" ftype="mothur.pair.dist"/> + <param name="name" value="amazon1.names" ftype="mothur.names"/> + <param name="label" value="unique,0.05,0.27,0.45"/> + <param name="type" value="yes"/> + <param name="group" value="amazon.groups" ftype="mothur.groups"/> + <param name="groups" value="forest,pasture"/> + <param name="count" value="amazon1.count_table" ftype="mothur.count_table"/> <!-- should be ignored as we also supplied name file --> + <output_collection name="names_out" count="8"> + <element name="0.27.pasture" md5="34f87d93fd9ebbefed9754f627f502fd" ftype="mothur.names"/> + </output_collection> + <expand macro="logfile-test"/> + </test> + <test><!-- test with count table --> + <param name="otu" value="amazon.an.list" ftype="mothur.list"/> + <param name="source" value="phylip"/> + <param name="dist" value="98_sq_phylip_amazon.dist" ftype="mothur.square.dist"/> + <param name="label" value="unique,0.05,0.27"/> + <param name="count" value="amazon1.count_table" ftype="mothur.count_table"/> + <output_collection name="count_out" count="3"> + <element name="0.27" md5="6b1afd712825af4bf8cb4ab8304df903" ftype="mothur.count_table"/> + </output_collection> + <expand macro="logfile-test"/> + </test> + </tests> + <help> +<![CDATA[ + +@MOTHUR_OVERVIEW@ + + +**Command Documenation** + +The get.oturep_ command generates a fasta-formatted sequence file containing only a representative sequence for each OTU. The opposite of the bin.seqs command. + +.. _get.oturep: http://www.mothur.org/wiki/Get.oturep + +v1.23.0: Updated to Mothur 1.33, added count and method parameter +]]> + </help> + <expand macro="citations"/> +</tool>