Mercurial > repos > iuc > mothur_consensus_seqs
comparison consensus.seqs.xml @ 0:fb865c8dc76f draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
| author | iuc |
|---|---|
| date | Fri, 24 Jun 2016 16:27:31 -0400 |
| parents | |
| children | fb0eb9393fa8 |
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| -1:000000000000 | 0:fb865c8dc76f |
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| 1 <tool profile="16.07" id="mothur_consensus_seqs" name="Consensus.seqs" version="@WRAPPER_VERSION@.0"> | |
| 2 <description>Find a consensus sequence for each OTU or phylotype</description> | |
| 3 <macros> | |
| 4 <import>macros.xml</import> | |
| 5 </macros> | |
| 6 <expand macro="requirements"/> | |
| 7 <expand macro="version_command"/> | |
| 8 <command detect_errors="aggressive"><![CDATA[ | |
| 9 ## create symlinks to input datasets | |
| 10 ln -s "$fasta" fasta.dat && | |
| 11 ln -s "$name" name.dat && | |
| 12 ln -s "$count" count.dat && | |
| 13 #if $perotu.use == "yes": | |
| 14 ln -s "$perotu.otu" perotu.otu.dat && | |
| 15 #end if | |
| 16 | |
| 17 echo 'consensus.seqs( | |
| 18 fasta=fasta.dat | |
| 19 #if $name: | |
| 20 ,name=name.dat | |
| 21 #end if | |
| 22 #if $cutoff: | |
| 23 ,cutoff=$cutoff | |
| 24 #end if | |
| 25 #if $perotu.use == "yes": | |
| 26 ,list=perotu.otu.dat | |
| 27 #if $perotu.label: | |
| 28 ,label=${ str($perotu.label).replace(",","-") } | |
| 29 #end if | |
| 30 #end if | |
| 31 #if $count: | |
| 32 ,count=count.dat | |
| 33 #end if | |
| 34 )' | |
| 35 | sed 's/ //g' ## mothur trips over whitespace | |
| 36 | mothur | |
| 37 ]]></command> | |
| 38 <inputs> | |
| 39 <param name="fasta" type="data" format="mothur.align" label="fasta - Sequences to Bin" help="Sequences must be aligned"/> | |
| 40 <param name="name" type="data" format="mothur.names" optional="true" label="name - Sequences Name reference"/> | |
| 41 <conditional name="perotu"> | |
| 42 <param name="use" type="select" label="Consensus sequence"> | |
| 43 <option value="no" selected="true">Single consensus sequence</option> | |
| 44 <option value="yes">Consensus sequence for each OTU in list</option> | |
| 45 </param> | |
| 46 <when value="yes"> | |
| 47 <param name="otu" type="data" format="mothur.list" label="list - OTU List"/> | |
| 48 <param name="label" type="select" optional="true" multiple="true" label="label - Select OTU Labels" help="By default all labels are used"> | |
| 49 <expand macro="labeloptions"/> | |
| 50 </param> | |
| 51 </when> | |
| 52 <when value="no"/> | |
| 53 </conditional> | |
| 54 <param name="cutoff" type="integer" value="" optional="True" min="1" max="100" label="Cutoff - set a percentage of sequences that support the base" help="For example: cutoff=95 would return the base that was supported by at least 95% of sequences. Must be between 1 and 100. Optional"/> | |
| 55 <param name="count" type="data" format="mothur.count_table" optional="true" label="Count - a count table" help="this file can be generated by count.seqs"/> | |
| 56 </inputs> | |
| 57 <outputs> | |
| 58 <expand macro="logfile-output"/> | |
| 59 <data name="summary" format="tabular" from_work_dir="fasta*.cons.summary" label="${tool.name} on ${on_string}: cons.summary"> | |
| 60 <filter>perotu['use'] == 'no'</filter> | |
| 61 </data> | |
| 62 <data name="cons_fasta" format="mothur.align" from_work_dir="fasta*.cons.fasta" label="${tool.name} on ${on_string}: cons.fasta"> | |
| 63 <filter>perotu['use'] == 'no'</filter> | |
| 64 </data> | |
| 65 <collection name="consensusfastas" type="list" label="${tool.name} on ${on_string}: consensus fasta files per OTU"> | |
| 66 <discover_datasets pattern=".*?\.(?P<designation>.*)\.cons\.fasta" format="mothur.align"/> | |
| 67 <filter>perotu['use'] == 'yes'</filter> | |
| 68 </collection> | |
| 69 <collection name="consensusnames" type="list" label="${tool.name} on ${on_string}: consensus names files per OTU"> | |
| 70 <discover_datasets pattern=".*?\.(?P<designation>.*)\.cons\.names" format="mothur.names"/> | |
| 71 <filter>perotu['use'] == 'yes'</filter> | |
| 72 </collection> | |
| 73 <collection name="consensussummaries" type="list" label="${tool.name} on ${on_string}: consensus summary files per OTU"> | |
| 74 <discover_datasets pattern=".*?\.(?P<designation>.*)\.cons\.summary" format="tabular"/> | |
| 75 <filter>perotu['use'] == 'yes'</filter> | |
| 76 </collection> | |
| 77 </outputs> | |
| 78 <tests> | |
| 79 <test><!-- test with default values --> | |
| 80 <param name="fasta" value="amazon.align_head" ftype="mothur.align"/> | |
| 81 <output name="summary" md5="61a0e170a8b881135421afd0cea8305a" ftype="tabular"/> | |
| 82 <output name="cons_fasta" md5="82f3475b61f240eba09597a409364c04" ftype="mothur.align"/> | |
| 83 <expand macro="logfile-test"/> | |
| 84 </test> | |
| 85 <test><!-- test with list file --> | |
| 86 <param name="fasta" value="amazon.align_head" ftype="mothur.align"/> | |
| 87 <param name="use" value="yes"/> | |
| 88 <param name="otu" value="amazon.align_head.list" ftype="mothur.list"/> | |
| 89 <param name="count" value="amazon.align_head.count_table" ftype="mothur.count_table"/> | |
| 90 <param name="labels" value="unique,0.30,0.33"/> | |
| 91 <output_collection name="consensusfastas" count="3"> | |
| 92 <element name="0.30" md5="a5c8c17814f02124194dba2e37f566c7" ftype="mothur.align"/> | |
| 93 </output_collection> | |
| 94 <output_collection name="consensusnames" count="3"> | |
| 95 <element name="0.30" md5="9de4f7e774e0c16eaf7cf6ffbba9d475" ftype="mothur.names"/> | |
| 96 </output_collection> | |
| 97 <output_collection name="consensussummaries" count="3"> | |
| 98 <element name="0.30" md5="d595234c6018dfcc1465e59d35d59205" ftype="tabular"/> | |
| 99 </output_collection> | |
| 100 <expand macro="logfile-test"/> | |
| 101 </test> | |
| 102 </tests> | |
| 103 <help> | |
| 104 <![CDATA[ | |
| 105 | |
| 106 @MOTHUR_OVERVIEW@ | |
| 107 | |
| 108 **Command Documenation** | |
| 109 | |
| 110 The consensus.seqs_ command can be used in 2 ways: create a consensus sequence from a fastafile, or with a listfile create a consensus sequence for each otu. Sequences must be aligned. | |
| 111 | |
| 112 .. _consensus.seqs: http://www.mothur.org/wiki/Consensus.seqs | |
| 113 ]]> | |
| 114 </help> | |
| 115 <expand macro="citations"/> | |
| 116 </tool> |
