Mercurial > repos > iuc > mapseq
annotate mapseq.xml @ 0:16f561c480bb draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
| author | iuc |
|---|---|
| date | Mon, 14 Oct 2024 12:27:57 +0000 |
| parents | |
| children |
| rev | line source |
|---|---|
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0
16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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1 <tool id="mapseq" name="MAPseq" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="21.05"> |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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2 <description>sequence read classification designed to assign taxonomy and OTU classifications</description> |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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3 <macros> |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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4 <token name="@TOOL_VERSION@">2.1.1b</token> |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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5 <token name="@VERSION_SUFFIX@">0</token> |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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6 </macros> |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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7 <xrefs> |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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8 <xref type="bio.tools">mapseq</xref> |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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9 </xrefs> |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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10 <requirements> |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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11 <requirement type="package" version="5.26">perl</requirement> |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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12 <requirement type="package" version="@TOOL_VERSION@">mapseq</requirement> |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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13 </requirements> |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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14 <command detect_errors="exit_code"><![CDATA[ |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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15 |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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16 #if $ref_db.db_source == "cached" and $ref_db.mapseq2biom.mapseq2biom == "no": |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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17 ln -s '${ref_db.db_cached.fields.path}'/*.fasta db.fasta && |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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18 ln -s '${ref_db.db_cached.fields.path}'/*.txt taxonomy.txt && |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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19 ln -s '${ref_db.db_cached.fields.path}'/*.mscluster db.fasta.mscluster && |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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20 #end if |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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21 |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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22 #if $ref_db.db_source == "cached" and $ref_db.mapseq2biom.mapseq2biom == "yes": |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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23 ln -s '${ref_db.db_cached.fields.path}'/*.fasta db.fasta && |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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24 ln -s '${ref_db.db_cached.fields.path}'/*.txt taxonomy.txt && |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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25 ln -s '${ref_db.db_cached.fields.path}'/*.mscluster db.fasta.mscluster && |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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26 ln -s '${ref_db.db_cached.fields.path}'/*.otu db.otu && |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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27 #end if |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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28 |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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29 #if $ref_db.db_source == "history" and $ref_db.mapseq2biom.mapseq2biom == "no": |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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30 ln -s '${ref_db.database}' db.fasta && |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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31 ln -s '${ref_db.taxonomy}' taxonomy.txt && |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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32 ln -s '${ref_db.mscluster}' db.fasta.mscluster && |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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33 #end if |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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34 |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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35 #if $ref_db.db_source == "history" and $ref_db.mapseq2biom.mapseq2biom == "yes": |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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36 ln -s '${ref_db.database}' db.fasta && |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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37 ln -s '${ref_db.taxonomy}' taxonomy.txt && |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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38 ln -s '${ref_db.mscluster}' db.fasta.mscluster && |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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39 ln -s '${ref_db.mapseq2biom.otu_table}' db.otu && |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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40 #end if |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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41 mapseq |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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42 -nthreads \${GALAXY_SLOTS:-8} |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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43 #if str($seed) != "" |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
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44 -seed '$seed' |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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45 #end if |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
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changeset
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46 -tophits '$tophits' |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
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changeset
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47 -topotus '$topotus' |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
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changeset
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48 -minscore '$minscore' |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
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49 -minid1 '$minid1' |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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50 -minid2 '$minid2' |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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51 -otulim '$otulim' |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
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52 -outfmt '$outfmt' |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
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changeset
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53 '$sequences' db.fasta taxonomy.txt > '$classifications' |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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54 |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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55 #if $ref_db.mapseq2biom.mapseq2biom == "yes": |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
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56 && |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
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57 perl '$__tool_directory__/mapseq2biom.pl' --otuTable db.otu --query '$classifications' --outfile '$otu_tsv' --taxid --notaxidfile '$otu_tsv_notaxid' |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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58 #if $ref_db.mapseq2biom.krona_input == 'yes': |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
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59 --krona '$krona_format' |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
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changeset
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60 #end if |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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61 #end if |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
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62 |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
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63 ]]></command> |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
changeset
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64 |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
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changeset
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65 <inputs> |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
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66 <param type="data" name="sequences" format="fasta" label="Input sequences" /> |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
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67 <conditional name="ref_db"> |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
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68 <param name="db_source" type="select" label="Use cached database or database from history" help=""> |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
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69 <option value="cached">Cached database</option> |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
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70 <option value="history">From history</option> |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
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71 </param> |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
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72 <when value="cached"> |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
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73 <param name="db_cached" type="select" label="Using built-in mapseq DB" help=""> |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
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74 <options from_data_table="mapseq_db"> |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
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75 <column name="value" index="0" /> |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
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76 <column name="name" index="1" /> |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
diff
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77 <column name="version" index="2" /> |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
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78 <column name="path" index="3" /> |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
iuc
parents:
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79 <filter type="sort_by" column="1"/> |
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16f561c480bb
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80 </options> |
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81 <validator type="no_options" message="A built-in mapseq DB is not available. Please ask the Galaxy admins to install one on the server." /> |
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82 </param> |
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83 <conditional name="mapseq2biom"> |
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84 <param type="select" name="mapseq2biom" label="Create OTU table" help="Creates a tab-separated OTU table (including taxonomy classification) that can be used to create BIOM files"> |
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85 <option value="yes">Yes</option> |
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86 <option value="no">No</option> |
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87 </param> |
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88 <when value="yes"> |
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16f561c480bb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mapseq commit 3652500c9a0b6d92f6dc254cea7dcfcc6522d842
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89 <param type="boolean" name="krona_input" truevalue="yes" falsevalue="no" label="Create taxon table for Krona" help="Generates a reads per taxon file suitable for the use with Krona" /> |
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90 </when> |
|
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91 <when value="no" /> |
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92 </conditional> |
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93 </when> |
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94 <when value="history"> |
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95 <param type="data" name="database" label="Database file (FASTA format)" format="fasta" /> |
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16f561c480bb
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96 <param type="data" name="taxonomy" label="Taxonomy file" format="tabular" /> |
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97 <param type="data" name="mscluster" label="Database cluster" format="txt" optional="true" /> |
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98 <conditional name="mapseq2biom"> |
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99 <param type="select" name="mapseq2biom" label="Create out of the MAPseq output a tab-separated output file?"> |
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100 <option value="yes">Yes</option> |
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101 <option value="no">No</option> |
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102 </param> |
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103 <when value="yes"> |
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104 <param type="data" name="otu_table" format="txt" label="OTU table" help="The OTU table produced for the taxonomies found in the reference databases that was used with MAPseq" /> |
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105 <param type="boolean" name="krona_input" truevalue="yes" falsevalue="no" help="Generates an output file suitable for the use with Krona" /> |
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106 </when> |
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107 <when value="no" /> |
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108 </conditional> |
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109 </when> |
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110 </conditional> |
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111 |
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112 <param argument="-seed" type="integer" label="Fix random seed" help="Sets a fixed integer seed value for random number generation, ensuring reproducible results" optional="true"/> |
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113 |
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114 <param argument="-tophits" type="integer" label="Top hits" help="Number of reference sequences to include in alignment phase" |
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115 value="20" min="1" max="200" /> |
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116 |
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117 <param argument="-topotus" type="integer" label="Top OTUs" help="Number of internal reference otus to include in alignment phase" |
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118 value="10" min="1" max="200" /> |
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119 |
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120 <param argument="-minscore" type="integer" label="Minimum score" |
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121 help="Minimum score cutoff to consider for a classification, should be reduced when searching very small sequences, i.e.: primer search" |
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122 value="30" min="1" max="50" /> |
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123 |
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124 <param argument="-minid1" type="integer" label="Minimum number of shared kmers" help="Minimum number of shared kmers to consider hit in second phase kmer search" |
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125 value="1" min="1" max="10" /> |
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126 |
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127 <param argument="-minid2" type="integer" label="Number of ref. sequences" help="Number of reference sequences to include in alignment phase" |
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128 value="1" min="1" max="10" /> |
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129 |
|
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130 <param argument="-otulim" type="integer" label="OTU limit" help="Minimum number of shared kmers to consider hit in alignment phase" |
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131 value="50" min="1" max="60" /> |
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132 |
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133 <param argument="-outfmt" type="select" label="Output format" help="The `confidences` format outputs confidence values for each of the taxonomic levels. "> |
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134 <option value="simple">simple</option> |
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135 <option value="confidences">confidences</option> |
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136 </param> |
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137 </inputs> |
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138 |
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139 <outputs> |
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140 <data format="tabular" name="classifications" label="Classification results"/> |
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141 <data name="otu_tsv" format="tabular" label="tab-output including taxIDs"> |
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16f561c480bb
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142 <filter>(ref_db['mapseq2biom']['mapseq2biom'] == "yes")</filter> |
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143 </data> |
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144 <data name="otu_tsv_notaxid" format="tabular" label="tab-output without taxIDs" > |
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145 <filter>(ref_db['mapseq2biom']['mapseq2biom'] == "yes")</filter> |
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146 </data> |
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147 <data name="krona_format" format="tabular" label="Krona input" > |
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148 <filter>(ref_db['mapseq2biom']['mapseq2biom'] == "yes" and ref_db['mapseq2biom']['krona_input'])</filter> |
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149 </data> |
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150 </outputs> |
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151 |
|
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152 <tests> |
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153 <test expect_num_outputs="1"> |
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154 <param name="db_source" value="history" /> |
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155 <param name="sequences" value="sequences.fasta"/> |
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156 <param name="database" value="mapseq_db/LSU_trimmed.fasta"/> |
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157 <param name="taxonomy" value="mapseq_db/slv_lsu_filtered2_trimmed.txt"/> |
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158 <param name="mscluster" value="mapseq_db/LSU_trimmed.fasta.mscluster"/> |
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159 <param name="mapseq2biom" value="no"/> |
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160 <output name="classifications" file="sequences.mapseq" sort="true"/> |
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161 <assert_command> |
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162 <has_text text="-seed" negate="true" /> |
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163 </assert_command> |
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164 </test> |
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165 <test expect_num_outputs="1"> |
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166 <param name="db_source" value="history" /> |
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167 <param name="sequences" value="sequences.fasta"/> |
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168 <param name="database" value="mapseq_db/LSU_trimmed.fasta"/> |
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169 <param name="taxonomy" value="mapseq_db/slv_lsu_filtered2_trimmed.txt"/> |
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170 <param name="mapseq2biom" value="no"/> |
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171 <output name="classifications" file="sequences.mapseq" sort="true"/> |
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172 <assert_command> |
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173 <has_text text="-seed" negate="true" /> |
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174 </assert_command> |
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175 </test> |
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176 <test expect_num_outputs="3"> |
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177 <param name="db_source" value="history" /> |
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178 <param name="sequences" value="sequences.fasta"/> |
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179 <param name="database" value="mapseq_db/LSU_trimmed.fasta"/> |
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180 <param name="taxonomy" value="mapseq_db/slv_lsu_filtered2_trimmed.txt"/> |
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181 <param name="mscluster" value="mapseq_db/LSU_trimmed.fasta.mscluster"/> |
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182 <param name="mapseq2biom" value="yes"/> |
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183 <param name="krona_input" value="no"/> |
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184 <param name="otu_table" value="mapseq_db/test.otu" /> |
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185 <param name="seed" value="12" /> |
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186 <output name="classifications" file="sequences.mapseq" sort="true"/> |
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187 <output name="otu_tsv" file="mapseq2biom/tab-output_including_taxIDs.tabular" /> |
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188 <output name="otu_tsv_notaxid" file="mapseq2biom/tab-output_without_taxIDs.tabular" /> |
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189 <assert_command> |
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190 <has_text text="-seed '12'" n="1" /> |
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191 </assert_command> |
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192 </test> |
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193 <test expect_num_outputs="4"> |
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194 <param name="db_source" value="history" /> |
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195 <param name="sequences" value="sequences.fasta"/> |
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196 <param name="database" value="mapseq_db/LSU_trimmed.fasta"/> |
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197 <param name="taxonomy" value="mapseq_db/slv_lsu_filtered2_trimmed.txt"/> |
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198 <param name="mscluster" value="mapseq_db/LSU_trimmed.fasta.mscluster"/> |
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199 <param name="mapseq2biom" value="yes"/> |
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200 <param name="krona_input" value="yes"/> |
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16f561c480bb
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201 <param name="otu_table" value="mapseq_db/test.otu" /> |
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202 <output name="classifications" file="sequences.mapseq" sort="true"/> |
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16f561c480bb
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203 <output name="otu_tsv" file="mapseq2biom/tab-output_including_taxIDs.tabular" /> |
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16f561c480bb
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204 <output name="otu_tsv_notaxid" file="mapseq2biom/tab-output_without_taxIDs.tabular" /> |
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205 <output name="krona_format" file="mapseq2biom/krona_input.tabular" /> |
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206 <assert_command> |
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207 <has_text text="-seed" negate="true" /> |
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208 </assert_command> |
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209 </test> |
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210 <test expect_num_outputs="1"> |
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211 <param name="db_source" value="cached" /> |
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212 <param name="db_cached" value="test_mapseq_db" /> |
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213 <param name="sequences" value="sequences.fasta"/> |
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214 <param name="mapseq2biom" value="no"/> |
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215 <output name="classifications" file="sequences.mapseq" sort="true"/> |
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216 <assert_command> |
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217 <has_text text="-seed" negate="true" /> |
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218 </assert_command> |
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219 </test> |
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220 <test expect_num_outputs="3"> |
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221 <param name="db_source" value="cached" /> |
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222 <param name="db_cached" value="test_mapseq_db" /> |
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223 <param name="sequences" value="sequences.fasta"/> |
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224 <param name="mapseq2biom" value="yes"/> |
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225 <param name="krona_input" value="no"/> |
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226 <output name="classifications" file="sequences.mapseq" sort="true"/> |
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227 <output name="otu_tsv" file="mapseq2biom/tab-output_including_taxIDs.tabular" /> |
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228 <output name="otu_tsv_notaxid" file="mapseq2biom/tab-output_without_taxIDs.tabular" /> |
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229 <assert_command> |
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230 <has_text text="-seed" negate="true" /> |
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231 </assert_command> |
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232 </test> |
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233 <test expect_num_outputs="4"> |
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234 <param name="db_source" value="cached" /> |
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235 <param name="db_cached" value="test_mapseq_db" /> |
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236 <param name="sequences" value="sequences.fasta"/> |
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237 <param name="mapseq2biom" value="yes"/> |
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238 <param name="krona_input" value="yes"/> |
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239 <param name="seed" value="12" /> |
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240 <output name="classifications" file="sequences.mapseq" sort="true"/> |
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241 <output name="otu_tsv" file="mapseq2biom/tab-output_including_taxIDs.tabular" /> |
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242 <output name="otu_tsv_notaxid" file="mapseq2biom/tab-output_without_taxIDs.tabular" /> |
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243 <output name="krona_format" file="mapseq2biom/krona_input.tabular" /> |
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244 <assert_command> |
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245 <has_text text="-seed '12'" n="1" /> |
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246 </assert_command> |
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247 </test> |
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248 </tests> |
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249 |
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250 <help><![CDATA[ |
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251 MAPseq |
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252 ====== |
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253 MAPseq is a set of fast and accurate sequence read classification tools |
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254 designed to assign taxonomy and OTU classifications to ribosomal RNA sequences. |
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255 This is done by using a reference set of full-length ribosomal RNA sequences |
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256 for which known taxonomies are known, and for which a set of high quality |
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257 OTU clusters has been previously generated. For each read, the best guess |
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258 and correspoding confidence in the assignment is shown at each taxonomic and OTU level. |
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259 |
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260 Mapseq2biom |
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261 =========== |
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262 This downstream script summaries the mapseq output as an OTU table |
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263 (including taxon information) as reads per OTU. This requires as input |
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264 an OTU to taxon mapping, for the taxonomy used to run the mapseq tool. |
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265 |
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266 |
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267 Example |
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268 ------- |
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269 |
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270 Mapseq output: |
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271 |
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272 :: |
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273 |
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274 # mapseq v1.2.3 (Oct 2 2018) |
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275 #query dbhit bitscore identity matches mismatches gaps query_start query_end dbhit_start dbhit_end strand ITS2 |
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276 test.1 355527192 204 0.9863636493682861 217 1 2 0 218 0 220 - sk__Eukaryota;k__Fungi;p__Ascomycota;c__Dothideomycetes;o__Pleosporales;f__Didymellaceae;g__Ectophoma;s__Ectophoma_multirostrata |
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277 test.2 555948006 248 0.8478803038597107 340 42 19 200 582 192 593 - sk__Eukaryota;k__Fungi |
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278 test.4 406352048 217 0.9127272963523865 251 22 2 106 381 169 442 - sk__Eukaryota;k__Fungi;p__ |
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279 |
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280 OTU to taxon mapping: |
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281 |
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282 :: |
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283 |
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284 1 sk__Eukaryota;k__Fungi |
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285 2 sk__Eukaryota;k__Fungi;p__;c__;o__;f__;g__;s__uncultured_fungus |
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286 3 sk__Eukaryota;k__Fungi;p__Ascomycota |
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287 4 sk__Eukaryota;k__Fungi;p__Ascomycota;c__Dothideomycetes;o__Pleosporales |
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288 5 sk__Eukaryota;k__Fungi;p__Ascomycota;c__Dothideomycetes;o__Pleosporales;f__Astrosphaeriellaceae;g__Pithomyces |
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289 6 sk__Eukaryota;k__Fungi;p__Ascomycota;c__Dothideomycetes;o__Pleosporales;f__Coniothyriaceae;g__Coniothyrium |
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290 7 sk__Eukaryota;k__Fungi;p__Ascomycota;c__Dothideomycetes;o__Pleosporales;f__Didymellaceae |
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291 8 sk__Eukaryota;k__Fungi;p__Ascomycota;c__Dothideomycetes;o__Pleosporales;f__Didymellaceae;g__Ectophoma |
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292 9 sk__Eukaryota;k__Fungi;p__Ascomycota;c__Dothideomycetes;o__Pleosporales;f__Didymellaceae;g__Ectophoma;s__Ectophoma_multirostrata |
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293 10 sk__Eukaryota;k__Fungi;p__Ascomycota;c__Dothideomycetes;o__Pleosporales;f__Didymosphaeriaceae;g__Paraconiothyrium;s__Paraconiothyrium_cyclothyrioides |
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294 |
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295 OTU output: |
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296 |
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297 :: |
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298 |
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299 # Constructed from biom file |
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300 # OTU ID label taxonomy |
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301 1 2.0 sk__Eukaryota;k__Fungi |
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302 9 1.0 sk__Eukaryota;k__Fungi;p__Ascomycota;c__Dothideomycetes;o__Pleosporales;f__Didymellaceae;g__Ectophoma;s__Ectophoma_multirostrata |
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303 |
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304 Taxon output for Krona: |
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305 |
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306 :: |
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307 |
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308 2 sk__Eukaryota k__Fungi |
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309 1 sk__Eukaryota k__Fungi p__Ascomycota c__Dothideomycetes o__Pleosporales f__Didymellaceae g__Ectophoma s__Ectophoma_multirostrata |
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310 |
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311 Source |
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312 ------ |
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313 * `GitHub <https://github.com/EBI-Metagenomics/pipeline-v5/blob/master/tools/RNA_prediction/mapseq2biom/mapseq2biom.pl>`_ |
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314 |
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315 License |
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316 ------- |
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317 * `Apache-2.0 license <https://raw.githubusercontent.com/EBI-Metagenomics/pipeline-v5/master/LICENSE>`_ |
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318 ]]></help> |
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319 <creator> |
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320 <person givenName="Rand" familyName="Zoabi" url="https://github.com/RZ9082"/> |
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321 <person givenName="Paul" familyName="Zierep" url="https://github.com/paulzierep"/> |
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322 </creator> |
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323 <citations> |
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324 <citation type="doi"> |
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325 10.1093/bioinformatics/btx517 |
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326 </citation> |
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327 <citation type="doi"> |
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328 10.1093/nar/gkac1080 |
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329 </citation> |
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330 </citations> |
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331 </tool> |
