Mercurial > repos > iuc > imagej2_binary_to_edm

annotate readme.md @ 2:c58b939c862a draft default tip

Find changesets by keywords (author, files, the commit message), revision number or hash, or revset expression.
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
author iuc
date Fri, 22 Jul 2016 23:15:40 -0400
parents
children
Ignore whitespace changes - Everywhere: Within whitespace: At end of lines:
rev   line source
2
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
1 Galaxy wrappers for ImageJ2 tools
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
2 ==================================
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
3
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
4 ImageJ2 is a new version of ImageJ for the next generation of multidimensional image data, with a focus on scientific imaging. Its central goal is to broaden the paradigm of ImageJ beyond the limitations of ImageJ 1.x, to support the next generation of multidimensional scientific imaging.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
5
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
6 Fiji is an image processing package. It can be described as a "batteries-included" distribution of ImageJ (and ImageJ2), bundling Java, Java3D and a lot of plugins organized into a coherent menu structure. Fiji compares to ImageJ as Ubuntu compares to Linux.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
7
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
8 More informations is available at:
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
9
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
10 * [http://fiji.sc/ImageJ2](http://fiji.sc/ImageJ2)
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
11 * [http://fiji.sc/Fiji](http://fiji.sc/Fiji)
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
12
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
13
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
14 Installation
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
15 ============
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
16
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
17 Galaxy tool wrappers use specified Fiji Lifeline versions available from [http://fiji.sc/Downloads](http://fiji.sc/Downloads). Galaxy should be able to automatically install this package.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
18
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
19 The wrappers are available at [https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2](https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2).
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
20
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
21
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
22 Use Docker
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
23 ==========
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
24
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
25 A docker image that installs Galaxy with these imaging tools is available at [https://github.com/bgruening/galaxy-imaging](https://github.com/bgruening/galaxy-imaging).
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
26
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
27
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
28 Using Fiji with Galaxy tools
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
29 ============================
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
30
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
31 Galaxy ImageJ2 tool wrappers generate a command line that calls a Python script, passing it a series of arguments including a Jython script named jython_script.py that resides in the same directory as the tool wrapper. During tool execution, the Python script will call ImageJ2 with the --headless argument to run without the ImageJ2 GUI. The Jython script is also passed to ImageJ2 along with all command line arguments that it expects. ImageJ2 will execute the Jython script, passing the expected arguments. The command line to run ImageJ2 from a Galaxy tool wrapper looks something like this:
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
32
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
33 `ImageJ2 --ij2 --headless --jython ~jython_script.py arg1, arg2, ...`
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
34
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
35 Each tool execution starts the ImageJ2 application within a Java virtual machine (JVM). When ImageJ2 is finished processing the Jython script, the results are either written to a file or returned to the calling Galaxy process. The JVM is shut down, and the Galaxy job terminates. This approach provides the ability to run ImageJ2 tools from Galaxy on any supported HPC environment.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
36
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
37 Of course, eliminating the ImageJ2 GUI restricts us to wrapping only those ImageJ2 plugins that do not require any GUI components (i.e., the ImageJ2 window manager). Plugins are written by an open community, so not all of them are written in such a way that they can be executed from the command line and produce useful results. For example, some plugins create one or more images that can only be accessed via calls to the ImageJ2 window manager, and running in headless mode eliminates the window manager as well as other GUI components.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
38
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
39 Those familiar with ImageJ2 will find differences with this general pattern for executing ImageJ2 tools within Galaxy. ImageJ2 accounts for user defined global preferences which are available to tools throughout the session, and an image can be uploaded and run through any number of available tools, saving only the final image. While Galaxy currently does not account for user preferences defined in ImageJ2, enhancements to the Galaxy framework are planned that will accomodate these kinds of settings (e.g., binary image options). Also, since Galaxy initiates a new ImageJ2 session with each tool execution, initial images are uploaded to ImageJ2 and resulting images are saved for each tool execution.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
40
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
41 The Galaxy ImageJ2 tools currently fall into the following categories. Additional tools will be added at a steady pace.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
42
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
43 Working with Pixels
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
44 ===================
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
45 These Galaxy tools wrap the ImageJ2 plugins that are available in the ImageJ2 Process → Math menu.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
46
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
47 * **Operate on pixels** - Applies a mathematical expression (add, subtract, multiply, etc.) to each pixel in the image. When the resulting pixel value overflows/underflows the legal range of the image's data type, the value is reset to the maximum/minimum value.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
48
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
49 Filters and Effects
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
50 ===================
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
51 These Galaxy tools wrap the ImageJ2 plugins that are available in the ImageJ2 Process menu.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
52
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
53 * **Smooth** - Blurs the image by replacing each pixel with the average of its 3x3 neighborhood.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
54 * **Sharpen** - Increases contrast and accentuates detail in the image, but may also accentuate noise.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
55 * **Find Edges** - Uses a Sobel edge detector to highlight sharp changes in intensity in the active image.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
56 * **Add shadow effect** - Produces a shadow effect, with light appearing to come from the selected direction (East, North, Northeast, Northwest, South, Southeast, Southwest and West).
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
57 * **Find Maxima** - Determines the local maxima in an image and creates a binary (mask-like) image of the same size with the maxima (or one segmented particle per maximum) marked.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
58 * **Enhance contrast** - Enhances image contrast by using either normalization (contrast stretching) or histogram equalization.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
59 * **Add or remove noise** - Adds specified noise to or removes noise from images.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
60
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
61 Binary Image Tools
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
62 ==================
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
63 These Galaxy tools wrap the ImageJ2 plugins that are available in the ImageJ2 Process → Binary menu.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
64
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
65 * **Convert to binary** - Converts an image into a binary (black and white) image.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
66 * **Adjust threshold** - Sets lower and upper threshold values, segmenting grayscale images into
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
67 features of interest and background.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
68 * **Watershed segmentation** - Automatically separates or cuts apart particles that touch.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
69 * **Analyze particles** - Analyzes the particles in a segmented binary image, providing information about
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
70 each particle in the image.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
71 * **Skeletonize images** - Uses the Skeletonize3D plugin to find the centerlines (”skeleton”) of objects in the input image. Skeletonize3d is a plugin written by Ignacio Arganda-Carreras that offers several advantages over the legacy skeletonization algorithm of ImageJ available in the Process → Binary → Skeletonize menu item. Skeletonize works only with binary 2D images. Skeletonize3D works with 8-bit 2D images and stacks, expecting the image to be binary. If not, Skeletonize3D considers all pixel values above 0 to be white (255). While Skeletonize↑ relies on Black background value, the output of Skeletonize3D always has a value of 255 at the skeleton and 0 at background pixels, independently of the Black background option.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
72 * **Analyze skeleton** - Tags all pixel/voxels in a skeleton image and then counts all its junctions,
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
73 triple and quadruple points and branches, and measures their average and maximum length.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
74 * **Convert binary image to EDM** - Converts a binary image into a 8-bit grayscale Euclidean Distance Map (EDM). Each foreground (nonzero) pixel in the binary image is assigned a value equal to its distance from the nearest background (zero) pixel.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
75
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
76 **Interpreting binary Images in ImageJ2**
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
77
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
78 Binary images are thresholded to only two values, typically 0 and 1, but often — as with ImageJ — 0 and 255, that represent black and white on an 8-bit scale.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
79
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
80 The interpretation of binary images is not universal. While some software packages will always perform binary operations on 255 values (or 1, or any non-zero value), ImageJ takes into account the foreground and background colors of the binary image.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
81
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
82 In ImageJ, the **Black background** global preference setting defines not only how new binary images will be created, but also how previously created images are interpreted. This means objects will be inferred on a image-per-image basis. As such, inverting the LUT (i.e., pixels with a value of zero are white and pixels with a value 255 are black) of a binary image without updating the black background option may lead to unexpected results. This issue can currently be avoided by properly selecting the **Black background** option available on all Galaxy binary image tools.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
83
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
84 BunwarpJ Plugin Tools
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
85 =====================
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
86 These Galaxy tools wrap the bUnwarpJ plugin [http://fiji.sc/BUnwarpJ](http://fiji.sc/BUnwarpJ).
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
87
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
88 * **Adapt an elastic transformation** - Adapts an elastic transformation to a new image size by transforming the
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
89 coefficients of a specified elastic transformation according to a real image factor.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
90 * **Align two images** - Performs a simultaneous registration of two images, A and B. Image A is elastically deformed
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
91 in order to look as similar as possible to image B, and, at the same time, the "inverse"
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
92 transformation (from B to A) is also calculated so a pseudo-invertibility of the final deformation
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
93 could be guaranteed. Two images are produced: the deformed versions of A and B images.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
94 * **Compare opposite elastic deformations** - Calculates the warping index of two opposite elast transformations, i.e. the average of the geometrical distance between every pixel and its version after applying both transformations (direct and inverse).
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
95 * **Compare elastic and raw deformation** - Calculates the warping index of an elastic transformation and a raw transformation.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
96 * **Compare two raw deformations** - Calculates the warping index of two raw transformations (same direction).
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
97 * **Compose two elastic transformations** - Composes two elastic transformations into a raw transformation.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
98 * **Compose two raw transformations** - Composes two raw transformations into another raw transformation.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
99 * **Compose a raw and an elastic transformation** - Composes a raw transformation and an elastic transformation
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
100 into a raw transformation.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
101 * **Convert elastic transformation to raw** - Converts an elastic (i.e., B-spline ) transformation file into a raw transformation file.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
102 * **Apply elastic transformation** - Applies an elastic transformation to an image, producing another image which is elastically
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
103 deformed according to the transformation.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
104 * **Apply raw transformation** - Applies a raw transformation to an image, producing another image which is deformed according
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
105 to the transformation.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
106
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
107 Other Tools
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
108 ===========
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
109 * **Create new image** - Creates a new image of a selected type, size, depth and format.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
110 * **Convert image format** - Converts the format of an input image file, producing an output image.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
111
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
112 Licence
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
113 =======
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
114
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
115 Fiji is released as open source under the GNU General Public License: [http://www.gnu.org/licenses/gpl.html](http://www.gnu.org/licenses/gpl.html)
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
116
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
117 Fiji builds on top of the ImageJ2 core, which is licensed under the permissive BSD 2-Clause license: [http://opensource.org/licenses/BSD-2-Clause](http://opensource.org/licenses/BSD-2-Clause)
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
118
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
119 Plugins and other components have their own licenses.
c58b939c862a planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8ea6a4271431c05c82b09c0d3e629b13e6ea7936
iuc
parents:
diff changeset
120