Mercurial > repos > iuc > imagej2_analyze_skeleton
diff imagej2_analyze_skeleton.xml @ 0:a989ca433027 draft
planemo upload commit 8f8692e83217a072a3ed928469621f1f606ab28a-dirty
| author | iuc |
|---|---|
| date | Tue, 04 Aug 2015 11:14:30 -0400 |
| parents | |
| children | dd8e4e000d2f |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/imagej2_analyze_skeleton.xml Tue Aug 04 11:14:30 2015 -0400 @@ -0,0 +1,112 @@ +<?xml version='1.0' encoding='UTF-8'?> +<tool id="imagej2_analyze_skeleton" name="Analyze skeleton" version="1.0.0"> + <description>with ImageJ2</description> + <macros> + <import>imagej2_macros.xml</import> + </macros> + <expand macro="fiji_20141125_requirements" /> + <command> +<![CDATA[ + python $__tool_directory__/imagej2_analyze_skeleton.py + --input "$input" + --input_datatype $input.ext + --prune_cycle_method $prune_cycle_method + --prune_ends $prune_ends + --calculate_largest_shortest_path $calculate_largest_shortest_path + --show_detailed_info $show_detailed_info + @max_heap_size_args@ + --jython_script $__tool_directory__/jython_script.py + --output "$output" +]]> + </command> + <inputs> + <param format="bmp,eps,gif,jpg,pcx,pgm,png,psd,tiff" name="input" type="data" label="Select grayscale image"/> + <param name="prune_cycle_method" type="select" label="Prune cycle method"> + <option value="none" selected="True">None</option> + <option value="shortest_branch">Shortest branch</option> + <option value="lowest_intensity_voxel">Lowest intensity voxel</option> + <option value="lowest_intensity_branch">Lowest intensity branch</option> + </param> + <param name="prune_ends" type="select" label="Prune ends"> + <option value="no" selected="True">No</option> + <option value="yes">Yes</option> + </param> + <param name="calculate_largest_shortest_path" type="select" label="Calculate largest shortest path"> + <option value="no" selected="True">No</option> + <option value="yes">Yes</option> + </param> + <param name="show_detailed_info" type="select" label="Show detailed info"> + <option value="no" selected="True">No</option> + <option value="yes">Yes</option> + </param> + <expand macro="max_heap_size_type_conditional" /> + </inputs> + <outputs> + <data name="output" format="tabular" label="${tool.name} on ${on_string}" /> + </outputs> + <tests> + <test> + <param name="input" value="skeletonized_blobs.gif" /> + <param name="input_datatype" value="gif" /> + <output name="output" file="basic.tabular" ftype="tabular" /> + </test> + <test> + <param name="input" value="skeletonized_clown.jpg" /> + <param name="input_datatype" value="jpg" /> + <param name="prune_cycle_method" value="shortest_branch" /> + <output name="output" file="shortest_branch_basic.tabular" ftype="tabular" /> + </test> + <test> + <param name="input" value="skeletonized_blobs.gif" /> + <param name="input_datatype" value="gif" /> + <param name="calculate_largest_shortest_path" value="yes" /> + <output name="output" file="largest_shortest_path_basic.tabular" ftype="tabular" /> + </test> + <test> + <param name="input" value="skeletonized_blobs.gif" /> + <param name="input_datatype" value="gif" /> + <param name="show_detailed_info" value="yes" /> + <output name="output" file="detailed.tabular" ftype="tabular" /> + </test> + <test> + <param name="input" value="skeletonized_clown.jpg" /> + <param name="input_datatype" value="jpg" /> + <param name="prune_cycle_method" value="shortest_branch" /> + <param name="calculate_largest_shortest_path" value="yes" /> + <param name="show_detailed_info" value="yes" /> + <output name="output" file="shortest_branch_all_yes.tabular" ftype="tabular" /> + </test> + </tests> + <help> +**What it does** + +<![CDATA[ +Tags all pixel/voxels in a skeleton image and then counts all its junctions, triple and quadruple +points and branches, and measures their average and maximum length. The "Skeletonize images" tool +produces skeleton images that can be used as input to this tool. Images that have not been +skeletonized will not result in errors, but the results may or may not be useful. The underlying +AnalyzeSkeleton Fiji plugin works on grayscale images, so other image types will automatically be +converted to grayscale before they are analyzed. This step is performed using the ImageJ **Make +Binary** command. + +The voxels are classified into three different categories depending on their 26 neighbors: + +- **End-point** voxels: if they have less than 2 neighbors. +- **Junction** voxels: if they have more than 2 neighbors. +- **Slab** voxels: if they have exactly 2 neighbors. + +----- + +**Options** + +- **Prune cycle method**: prune the possible loops in the skeleton. +- **Prune ends**: prunes any branch that ends in an end-point. +- **Calculate largest shortest path**: calculate the largest shortest path of each skeleton using the APSP (all pairs shortest path). +- **Show detailed info**: produces information about the branches of each skeleton in the image. +]]> + </help> + <citations> + <citation type="doi">10.1002/jemt.20829</citation> + <citation type="doi">10.1038/nmeth.2102</citation> + </citations> +</tool>
