annotate hybpiper.xml @ 0:91a16438e849 draft default tip

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
author iuc
date Sat, 23 Sep 2023 16:49:12 +0000
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Ignore whitespace changes - Everywhere: Within whitespace: At end of lines:
rev   line source
0
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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1 <tool id="hybpiper" name="HybPiper" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@">
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2 <description>Analyse targeted sequence capture data</description>
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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3 <macros>
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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4 <import>macros.xml</import>
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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5 </macros>
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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6 <expand macro="xrefs"/>
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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7 <expand macro="requirements"/>
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8 <command detect_errors="exit_code"><![CDATA[
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9
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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10 ## sample name checking
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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11 #import re
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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12 #def check_sample_name($sample_name):
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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13 #if re.search(r'[^A-Za-z0-9_\-]', $sample_name):
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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14 printf '%s\n'
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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15 'ERROR: special characters detected in sample identifier.'
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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16 'Identifiers may only contain letters, numbers, underscores and hyphens.'
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17 'Check the identifier for the following sample:'
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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18 '${sample_name}'
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19 1>&2
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20 &&
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21 exit 1
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22 &&
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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23 #end if
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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24 #end def
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25
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26 ## set up files
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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27 ln -s '${targetfile_dna}' ./target_file.fasta
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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28 &&
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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29
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30 ###############################
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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31 ## hybpiper check_targetfile ##
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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32 ###############################
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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33
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34 #if str( $job_conditional.hybpiper_job ) == "check_and_fix_targetfile":
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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35 hybpiper check_targetfile
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36 --targetfile_dna target_file.fasta
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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37 &&
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38
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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39 mv fix_targetfile*.ctl hybpiper.ctl
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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40 &&
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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41
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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42 hybpiper fix_targetfile
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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43 --targetfile_dna target_file.fasta
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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44 --allow_gene_removal
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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45 hybpiper.ctl
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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46 &&
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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47
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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48 #######################
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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49 ## hybpiper assemble ##
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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50 #######################
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51
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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52 #elif str( $job_conditional.hybpiper_job ) == "assemble":
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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53 #set sample_prefix = str($job_conditional.paired_input.element_identifier)
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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54
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55 $check_sample_name($sample_prefix)
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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56
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57 hybpiper assemble
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58 --readfiles
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59 '${job_conditional.paired_input.forward}'
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60 '${job_conditional.paired_input.reverse}'
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61 --targetfile_dna target_file.fasta
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62 --diamond
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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63 --cpu \${GALAXY_SLOTS:-1}
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64 --prefix '${sample_prefix}'
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65 &&
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66
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67 tar -cvf '${hybpiper_archive}' --directory='${sample_prefix}' .
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68 &&
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69
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70 #######################################
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71 ## hybpiper stats/retrieve_sequences ##
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72 #######################################
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73
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74 #elif str( $job_conditional.hybpiper_job ) == "stats":
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75
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76 ## check logic of requested items
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77 #unless $job_conditional.stats_type_select or $job_conditional.sequence_type_select:
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78 printf '%s\n'
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79 'ERROR: No outputs selected.'
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80 1>&2
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81 &&
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82 exit 1
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83 &&
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84 #end unless
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85 #if $job_conditional.heatmap and not $job_conditional.stats_type_select:
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86 printf '%s\n'
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87 'ERROR: heatmap requested, but no stats selected.'
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88 1>&2
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89 &&
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90 exit 1
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91 &&
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92 #end if
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93
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94 #for $sample in $job_conditional.hybpiper_results
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95 #set sample_prefix = str($sample.element_identifier)
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96
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97 $check_sample_name($sample_prefix)
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98
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99 mkdir -p '${sample_prefix}'
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100 &&
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101
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102 tar -xf '${sample}' -C '${sample_prefix}'
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103 &&
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104
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105 echo '${sample_prefix}' >> namelist.txt
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106 &&
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107 #end for
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108
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109 ## Produce a stats file for each requested output type
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110 #for $stats_output in $job_conditional.stats_type_select:
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111 hybpiper stats
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112 --targetfile_dna target_file.fasta
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113 --stats_filename 'stats.${stats_output}'
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114 --seq_lengths_filename 'seq_lengths.${stats_output}'
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115 '${stats_output}'
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116 namelist.txt
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117 &&
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118
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119 ## Produce heatmaps if selected
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120 #if $job_conditional.heatmap:
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121 hybpiper recovery_heatmap
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122 --heatmap_filename 'heatmap.${stats_output}'
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123 --heatmap_filetype svg
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124 'seq_lengths.${stats_output}.tsv'
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125 &&
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126 #end if
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127 #end for
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128
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129 ## Produce sequences for each requested type
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130 #for $sequence_output in $job_conditional.sequence_type_select:
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131 mkdir 'fasta.${sequence_output}'
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132 &&
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133 hybpiper retrieve_sequences
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134 --targetfile_dna target_file.fasta
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135 --sample_names namelist.txt
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136 --fasta_dir 'fasta.${sequence_output}'
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137 '${sequence_output}'
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138 &&
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139 #end for
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140 #end if
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141
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142 wait
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143
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144 ]]></command>
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145
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146 <inputs>
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147 <param argument="--targetfile_dna" type="data" format="fasta" label="Target file" help="Target file in FASTA format" />
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148
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149 <conditional name="job_conditional">
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150 <param name="hybpiper_job" type="select" label="Type of hybpiper run">
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151 <option value="check_and_fix_targetfile">Check and fix targetfile</option>
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152 <option value="assemble" selected="true">Assemble target loci</option>
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153 <option value="stats">Extract sequences and/or stats from Hybpiper runs</option>
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154 </param>
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155
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156 <when value="check_and_fix_targetfile"/>
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157
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158 <when value="assemble">
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159 <param name="paired_input" format="fastqsanger" type="data_collection" collection_type="paired" label="Input reads" help="Your reads must be in a paired collection. See below for more information." />
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160 </when>
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161
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162 <when value="stats">
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163 <param name="hybpiper_results" type="data_collection" collection_type="list" format="tar" multiple="true" label="Results from Hybpiper assemble runs" />
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164 <param name="stats_type_select" type="select" label="Choose statistics to report" display="checkboxes" multiple="true" optional="true">
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165 <option value="gene" selected="true">Gene</option>
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166 <option value="supercontig">Supercontig</option>
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167 </param>
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168 <param name="heatmap" type="boolean" checked="false" label="Produce a heatmap for each of the selected statistics" />
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169 <param name="sequence_type_select" type="select" display="checkboxes" label="Choose sequences to extract" multiple="true" optional="true">
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170 <option value="dna" selected="true">DNA</option>
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171 <option value="aa">Amino acid</option>
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172 <option value="intron">Intron</option>
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173 <option value="supercontig">Supercontig</option>
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174 </param>
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175 </when>
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176 </conditional>
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177 </inputs>
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178
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179 <outputs>
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180 <!-- check_targetfile output -->
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181 <data name="fixed_targetfile" label="${targetfile_dna.element_identifier} (fixed)" format="fasta" from_work_dir="target_file_fixed.fasta">
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182 <filter>job_conditional['hybpiper_job'] == 'check_and_fix_targetfile'</filter>
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183 </data>
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184 <collection type="list" name="output_targetfile" label="Hybpiper logs for ${targetfile_dna.element_identifier}">
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185 <data name="targetfile_ctl_file" label="Hybpiper .ctl file for ${on_string}" format="txt" from_work_dir="hybpiper.ctl" />
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186 <data name="targetfile_report" label="Hybpiper targetfile report" format="tabular" from_work_dir="fix_targetfile_report.tsv" />
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187 <filter>job_conditional['hybpiper_job'] == 'check_and_fix_targetfile'</filter>
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188 </collection>
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189
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190 <!-- assemble output -->
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191 <data name="hybpiper_archive" format="tar">
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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192 <filter>job_conditional['hybpiper_job'] == 'assemble'</filter>
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193 </data>
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194
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195 <!-- stats / stats output -->
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196 <collection name="hybpiper_stats" type="list" label="Hybpiper statistics">
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197 <data name="stats_gene" label="Hybpiper statistics (gene)" format="tabular" from_work_dir="stats.gene.tsv">
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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198 <actions>
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199 <action name="column_names" type="metadata" default="Name,NumReads,ReadsMapped,PctOnTarget,GenesMapped,GenesWithContigs,GenesWithSeqs,GenesAt25pct,GenesAt50pct,GenesAt75pct,GenesAt150pct,ParalogWarningsLong,ParalogWarningsDepth,GenesWithoutStitchedContigs,GenesWithStitchedContigs,GenesWithStitchedContigsSkipped,GenesWithChimeraWarning,TotalBasesRecovered" />
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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200 </actions>
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201 </data>
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202 <data name="stats_supercontig" label="Hybpiper statistics (supercontig)" format="tabular" from_work_dir="stats.supercontig.tsv">
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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203 <actions>
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204 <action name="column_names" type="metadata" default="Name,NumReads,ReadsMapped,PctOnTarget,GenesMapped,GenesWithContigs,GenesWithSeqs,GenesAt25pct,GenesAt50pct,GenesAt75pct,GenesAt150pct,ParalogWarningsLong,ParalogWarningsDepth,GenesWithoutStitchedContigs,GenesWithStitchedContigs,GenesWithStitchedContigsSkipped,GenesWithChimeraWarning,TotalBasesRecovered" />
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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205 </actions>
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206 </data>
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207 <data name="seqlengths_gene" label="Assembled sequence lengths (gene)" format="tabular" from_work_dir="seq_lengths.gene.tsv"/>
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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208 <data name="seqlengths_supercontig" label="Assembled sequence lengths (supercontig)" format="tabular" from_work_dir="seq_lengths.supercontig.tsv">
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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209 </data>
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210 <filter>job_conditional['hybpiper_job'] == 'stats' and ('gene' in job_conditional['stats_type_select'] or 'supercontig' in job_conditional['stats_type_select'])</filter>
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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211 </collection>
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212
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213 <!-- stats/heatmap output -->
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214 <collection name="hybpiper_heatmaps" type="list" label="Hybpiper heatmaps">
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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215 <discover_datasets pattern="heatmap\.(?P&lt;designation&gt;.+)\.svg" format="svg" recurse="false" />
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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216 <filter>job_conditional['hybpiper_job'] == 'stats' and job_conditional['heatmap'] and job_conditional['heatmap'] is true</filter>
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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217 </collection>
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218
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219 <!-- stats/sequences output -->
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220 <collection name="dna_sequences" type="list" label="DNA sequences">
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221 <discover_datasets pattern="(?P&lt;designation&gt;.+)\.FNA" format="fasta" directory="fasta.dna" recurse="false" />
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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222 <filter>job_conditional['hybpiper_job'] == 'stats' and 'dna' in job_conditional['sequence_type_select']</filter>
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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223 </collection>
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224 <collection name="aa_sequences" type="list" label="Amino acid sequences">
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225 <discover_datasets pattern="(?P&lt;designation&gt;.+)\.FAA" format="fasta" directory="fasta.aa" recurse="false" />
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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226 <filter>job_conditional['hybpiper_job'] == 'stats' and 'aa' in job_conditional['sequence_type_select']</filter>
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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227 </collection>
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228 <collection name="intron_sequences" type="list" label="Intron sequences">
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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229 <discover_datasets pattern="(?P&lt;designation&gt;.+)\.fasta" format="fasta" directory="fasta.intron" recurse="false" />
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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230 <filter>job_conditional['hybpiper_job'] == 'stats' and 'intron' in job_conditional['sequence_type_select']</filter>
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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231 </collection>
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232 <collection name="supercontig_sequences" type="list" label="Supercontig sequences">
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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233 <discover_datasets pattern="(?P&lt;designation&gt;.+)\.fasta" format="fasta" directory="fasta.supercontig" recurse="false" />
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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234 <filter>job_conditional['hybpiper_job'] == 'stats' and 'supercontig' in job_conditional['sequence_type_select']</filter>
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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235 </collection>
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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236
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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237 <!-- dummy output, in case the user deselects everything -->
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238 <data name="dummy_output" label="Stats or sequences from Hybpiper runs" from_work_dir="namelist.txt" format="txt">
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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239 <filter>job_conditional['hybpiper_job'] == 'stats' and not (job_conditional['stats_type_select'] or job_conditional['sequence_type_select']) </filter>
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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240 </data>
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241
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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242 </outputs>
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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243 <tests>
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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244
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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245 <!-- test1: check and fix targetfile -->
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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246 <test expect_num_outputs="4">
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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247 <param name="targetfile_dna" value="test_targets.fasta.gz"/>
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248 <conditional name="job_conditional">
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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249 <param name="hybpiper_job" value="check_and_fix_targetfile"/>
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250 </conditional>
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251 <output name="fixed_targetfile" file="test1_out.fasta"/>
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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252 <output_collection name="output_targetfile" type="list" count="2">
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253 <element name="targetfile_ctl_file" file="test1_out.ctl"/>
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254 <element name="targetfile_report" file="test1_out.tsv"/>
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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255 </output_collection>
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256 </test>
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257
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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258 <!-- test2: assemble with paired collection -->
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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259 <!-- Not possible to test stats unless element_identifier can be set. -->
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260 <test expect_failure="true">
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261 <param name="targetfile_dna" value="test_targets.fasta.gz"/>
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262 <conditional name="job_conditional">
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263 <param name="hybpiper_job" value="assemble"/>
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264 <param name="paired_input">
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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265 <collection type="paired">
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266 <element name="forward" ftype="fastqsanger.gz" value="NZ874_R1_test.fastq.gz" />
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267 <element name="reverse" ftype="fastqsanger.gz" value="NZ874_R2_test.fastq.gz" />
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268 </collection>
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269 </param>
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270 </conditional>
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271 <!-- <output name="hybpiper_archive">
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272 <assert_contents>
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273 <has_size value="2386944" delta="200000" />
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274 </assert_contents>
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275 </output> -->
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276 </test>
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277
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278 <!-- test3: all stats output -->
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279 <test expect_num_outputs="10">
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280 <param name="targetfile_dna" value="test_targets.fasta.gz"/>
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281 <conditional name="job_conditional">
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282 <param name="hybpiper_job" value="stats"/>
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283 <param name="hybpiper_results" >
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284 <collection type="list">
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285 <element name="NZ874" value="NZ874.tar.gz" />
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286 </collection>
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287 </param>
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288 <param name="stats_type_select" value="gene,supercontig"/>
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289 <param name="heatmap" value="true"/>
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290 <param name="sequence_type_select" value="dna,aa,intron,supercontig"/>
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291 </conditional>
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292 <output_collection name="hybpiper_stats" type="list" count="4" />
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293 <output_collection name="hybpiper_heatmaps" type="list" count="2">
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294 </output_collection>
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295 <output_collection name="dna_sequences" type="list" count="13">
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296 </output_collection>
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297 <output_collection name="aa_sequences" type="list" count="13">
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298 </output_collection>
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299 <output_collection name="intron_sequences" type="list" count="13">
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300 </output_collection>
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301 <output_collection name="supercontig_sequences" type="list" count="13">
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302 </output_collection>
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303 </test>
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304
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305 <!-- test4: no output selected -->
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306 <test expect_failure="true">
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307 <param name="targetfile_dna" value="test_targets.fasta.gz"/>
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308 <conditional name="job_conditional">
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309 <param name="hybpiper_job" value="stats"/>
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310 <param name="hybpiper_results" >
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311 <collection type="list">
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312 <element name="NZ874" value="NZ874.tar.gz" />
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313 </collection>
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314 </param>
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315 <param name="stats_type_select" value=""/>
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316 <param name="heatmap" value="true"/>
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317 <param name="sequence_type_select" value=""/>
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318 </conditional>
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319 </test>
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320
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321 </tests>
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322 <help><![CDATA[
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323
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324 Using HybPiper on Galaxy
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325 ------------------------
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326
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327 Input
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328 ~~~~~
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329
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330 On Galaxy, **you have to use paired collections as input** for
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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331 HybPiper assemblies. HybPiper relies on the directory hierarchy it creates for each
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332 sample during assembly. The hierarchy is based on the name of the
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333 sample, which you provide to Galaxy as the identifier in the collection.
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334
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335 Using paired collections
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336 ~~~~~~~~~~~~~~~~~~~~~~~~
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337
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338 If you have your sequencing reads in individual datasets, you can easily organise them into a paired
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339 collection. See the Galaxy training material on `using dataset
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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340 collections <https://gxy.io/GTN:T00146>`__
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341 for a step-by-step guide.
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342
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343 **Note**: because HybPiper uses sample
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344 identifiers to create directories, you **can't use special characters**
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345 in your sample identifiers. The only allowed characters are letters,
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346 numbers, underscores and hyphens.
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347
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348 You can't use single-end and unpaired reads as input to Hybpiper on Galaxy.
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349
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350 Running HybPiper
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351 ~~~~~~~~~~~~~~~~
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352
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353 The following HybPiper analyses are available on Galaxy:
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354
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355 1. Check your target file and fix issues (optional)
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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356 2. Assemble target loci per-sample
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357 3. Extract sequences and summary statistics
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358
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359 Use the *Type of hybpiper run* drop-down to select an analysis.
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360
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361 .. class:: infomark
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362
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363 What it does
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364 ------------
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365
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366 HybPiper was designed for processing targeted sequence capture data. In
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367 targeted sequence capture, DNA sequencing libraries are enriched for
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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368 gene regions of interest. This is used for sequencing many loci
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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369 simultaneously based on bait sequences.
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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370
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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371 HybPiper is a suite of scripts that wrap and connect other tools to
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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372 extract target sequences from the sequencing reads. The HybPiper
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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373 pipeline starts with high-throughput sequencing reads (for example from
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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374 Illumina MiSeq), and assigns them to target genes using DIAMOND. The
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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375 reads are distributed to separate directories, where they are assembled
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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376 separately using SPAdes. The main output is a collection of FASTA files
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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377 of the (in frame) CDS portion of the sample for each target region. You
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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378 can also generate a separate collections of files with the translated
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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379 protein sequences, the intronic regions flanking each exon, and putative
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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380 paralog sequences.
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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381
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382 For more information, please see `the HybPiper
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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383 wiki <https://github.com/mossmatters/HybPiper/wiki>`__.
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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384
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385
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386 ]]></help>
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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387 <expand macro="citations"/>
91a16438e849 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hybpiper commit b439a8bebdd20955135572a15672a12a166d7ff8
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388 </tool>