Mercurial > repos > iuc > hicexplorer_chicplotviewpoint
comparison chicPlotViewpoint.xml @ 0:8a71b4ddcb9f draft default tip
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hicexplorer commit 9e4e8de0e003fc15f5bae3648232c2a8a57e6391"
| author | iuc |
|---|---|
| date | Mon, 09 Dec 2019 10:32:05 +0000 |
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| children |
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| -1:000000000000 | 0:8a71b4ddcb9f |
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| 1 <tool id="hicexplorer_chicplotviewpoint" name="@BINARY@" version="@WRAPPER_VERSION@.0"> | |
| 2 <description>creates plots for viewpoints</description> | |
| 3 <macros> | |
| 4 <token name="@BINARY@">chicPlotViewpoint</token> | |
| 5 <import>macros.xml</import> | |
| 6 </macros> | |
| 7 <expand macro="requirements" /> | |
| 8 <command detect_errors="exit_code"><![CDATA[ | |
| 9 #import re | |
| 10 | |
| 11 mkdir interactionFiles && | |
| 12 mkdir differentialFolder && | |
| 13 mkdir significantFolder && | |
| 14 mkdir plots && | |
| 15 | |
| 16 | |
| 17 #for $file in $interactionFiles: | |
| 18 #set identifier = @ESCAPE_IDENTIFIER_FILE@ | |
| 19 ln -s '$file' 'interactionFiles/$identifier' && | |
| 20 #end for | |
| 21 | |
| 22 #if $differentialFiles: | |
| 23 #for $file in $differentialFiles: | |
| 24 #set identifier = @ESCAPE_IDENTIFIER_FILE@ | |
| 25 ln -s '$file' 'differentialFolder/$identifier' && | |
| 26 #end for | |
| 27 #end if | |
| 28 | |
| 29 #if $significantFiles: | |
| 30 #for $file in $significantFiles: | |
| 31 #set identifier = @ESCAPE_IDENTIFIER_FILE@ | |
| 32 ln -s '$file' 'significantFolder/$identifier' && | |
| 33 #end for | |
| 34 #end if | |
| 35 | |
| 36 #if $batchmode_conditional.batchmode_selector == 'optionSinglemode': | |
| 37 #set $interactonfileslist = ' '.join([ '\'interactionFiles/%s\'' % @ESCAPE_IDENTIFIER_FILE@ for $file in $interactionFiles ]) | |
| 38 #set $differentialfileslist = ' '.join([ '\'differentialFolder/%s\'' % @ESCAPE_IDENTIFIER_FILE@ for $file in $differentialFiles ]) | |
| 39 #set $significantfileslist = ' '.join([ '\'significantFolder/%s\'' % @ESCAPE_IDENTIFIER_FILE@ for $file in $significantFiles ]) | |
| 40 | |
| 41 #end if | |
| 42 | |
| 43 @BINARY@ | |
| 44 #if $batchmode_conditional.batchmode_selector == 'optionSinglemode': | |
| 45 --interactionFile $interactonfileslist | |
| 46 #if $differentialFiles: | |
| 47 --differentialTestResult $differentialfileslist | |
| 48 #end if | |
| 49 | |
| 50 #if $significantFiles: | |
| 51 --significantInteractions $significantfileslist | |
| 52 #end if | |
| 53 | |
| 54 -o out_plot.$image_file_format | |
| 55 #else: | |
| 56 --interactionFile $batchmode_conditional.interactionFilesOrderFile | |
| 57 --interactionFileFolder interactionFiles | |
| 58 #if $batchmode_conditional.differentialFilesOrderFile: | |
| 59 --differentialTestResult $batchmode_conditional.differentialFilesOrderFile | |
| 60 --differentialTestResultsFolder differentialFolder | |
| 61 #end if | |
| 62 #if $batchmode_conditional.significantInteractionFilesOrderFile: | |
| 63 --significantInteractions $batchmode_conditional.significantInteractionFilesOrderFile | |
| 64 --significantInteractionFileFolder significantFolder | |
| 65 #end if | |
| 66 --plotSampleNumber $batchmode_conditional.computeSampleNumber | |
| 67 | |
| 68 --batchMode | |
| 69 --outputFolder plots | |
| 70 --outputFormat $image_file_format | |
| 71 | |
| 72 | |
| 73 #end if | |
| 74 | |
| 75 --range $rangeUpstream $rangeDownstream | |
| 76 #if $backgroundModelFile: | |
| 77 --backgroundModelFile $backgroundModelFile | |
| 78 #end if | |
| 79 | |
| 80 #if $dpi: | |
| 81 --dpi $dpi | |
| 82 #end if | |
| 83 | |
| 84 #if $maxPValue: | |
| 85 --maxPValue $maxPValue | |
| 86 #end if | |
| 87 | |
| 88 #if $minPValue: | |
| 89 --minPValue $minPValue | |
| 90 #end if | |
| 91 | |
| 92 --binResolution $resolution | |
| 93 #if $colormap: | |
| 94 --colorMapPvalue $colormap | |
| 95 #end if | |
| 96 | |
| 97 $pvalue | |
| 98 #if $xFold: | |
| 99 --xFold $xFold | |
| 100 #end if | |
| 101 --colorList $colorList | |
| 102 --threads @THREADS@ | |
| 103 | |
| 104 #if $batchmode_conditional.batchmode_selector == 'optionSinglemode': | |
| 105 && mv out_plot.$image_file_format plots/out_plot.$image_file_format | |
| 106 #end if | |
| 107 ]]></command> | |
| 108 <inputs> | |
| 109 <param name='interactionFiles' type="data" format="interval" label="Interaction files" multiple="true"/> | |
| 110 <param name='differentialFiles' type="data" format="interval" label="Rejected H0 files" multiple="true" optional='true'/> | |
| 111 <param name='significantFiles' type="data" format="interval" label="Significant interaction files" multiple="true" optional='true'/> | |
| 112 | |
| 113 <param name="rangeUpstream" type="integer" value="500000" label="Upstream range" help='Defines the region upstream of a reference point which should be considered in the analysis.' /> | |
| 114 <param name="rangeDownstream" type="integer" value="500000" label="Downstream range" help='Defines the region upstream of a reference point which should be considered in the analysis.' /> | |
| 115 <param argument="--backgroundModelFile" type="data" format='tabular' | |
| 116 label="Background model" | |
| 117 help="The background file computed by chicViewpointBackgroundModel" optional='true'/> | |
| 118 | |
| 119 <conditional name="batchmode_conditional"> | |
| 120 <param name="batchmode_selector" type="select" label="Compute files individually or in batch mode"> | |
| 121 <option value="optionBatchmode">Batch processing</option> | |
| 122 <option value="optionSinglemode" selected="True">Single file processing</option> | |
| 123 </param> | |
| 124 <when value="optionBatchmode"> | |
| 125 <param name='interactionFilesOrderFile' type="data" format="txt" label="Interaction file order"/> | |
| 126 <param name='differentialFilesOrderFile' type="data" format="txt" label="Rejected H0 file order" optional='true'/> | |
| 127 <param name='significantInteractionFilesOrderFile' type="data" format="txt" label="Significant file order" optional='true'/> | |
| 128 <param name='computeSampleNumber' type="integer" value='2' label="Plot n sample in one plot"/> | |
| 129 | |
| 130 </when> | |
| 131 <when value="optionSinglemode"> | |
| 132 </when> | |
| 133 </conditional> | |
| 134 | |
| 135 | |
| 136 <param argument="--maxPValue" type="float" optional="true" label="Max p-value" help="Maximum value of the plotted p-value."/> | |
| 137 <param argument="--minPValue" type="float" optional="true" label="Min p-value" help="Minimum value of the plotted p-value."/> | |
| 138 <param name='resolution' type='integer' value='1000' label='Resolution of cHi-C data' help='Resolution of the bin in genomic units. Values are usually e.g. 1000 for a 1kb, 5000 for a 5kb or 10000 for a 10kb resolution.' optional='true'/> | |
| 139 <param name='pvalue' type='boolean' truevalue='--pValue' falsevalue="" checked="false" label="Plot p-values as a colorbar"/> | |
| 140 <expand macro="colormap" /> | |
| 141 | |
| 142 <param name='xFold' type='float' value='' label='X-fold mean background' help='Plot x-fold region for the mean background.' optional='true'/> | |
| 143 | |
| 144 <param name="image_file_format" type="select" label="Image output format"> | |
| 145 <option value="png" selected="True">png</option> | |
| 146 <option value="svg">svg</option> | |
| 147 <option value="pdf">pdf</option> | |
| 148 </param> | |
| 149 <param name='dpi' type='integer' label='DPI for image' help='Change the default resolution of the plot.' optional='true'/> | |
| 150 <param name='colorList' type='text' value='g b c m y k' label='Color list' help='Colorlist for the viewpoint lines. For all available colors please check matplotlib documentation: https://matplotlib.org/3.1.0/gallery/color/named_colors.html' optional='true'/> | |
| 151 | |
| 152 | |
| 153 </inputs> | |
| 154 <outputs> | |
| 155 <collection name="plotsCollection" type="list" label="cHi-C viewpoint plots"> | |
| 156 <discover_datasets pattern="__name_and_ext__" directory="plots" /> | |
| 157 </collection> | |
| 158 | |
| 159 </outputs> | |
| 160 <tests> | |
| 161 <test> | |
| 162 <param name="interactionFiles" value="cHi-C/chicViewpoint/output_1/FL-E13-5_chr1_chr1_14300280_14300280_Eya1.bed,cHi-C/chicViewpoint/output_1/FL-E13-5_chr1_chr1_4487435_4487435_Sox17.bed,cHi-C/chicViewpoint/output_1/FL-E13-5_chr1_chr1_19093103_19093103_Tfap2d.bed,cHi-C/chicViewpoint/output_1/MB-E10-5_chr1_chr1_4487435_4487435_Sox17.bed,cHi-C/chicViewpoint/output_1/MB-E10-5_chr1_chr1_14300280_14300280_Eya1.bed,cHi-C/chicViewpoint/output_1/MB-E10-5_chr1_chr1_19093103_19093103_Tfap2d.bed"/> | |
| 163 <param name="differentialFiles" value="cHi-C/chicDifferentialTest/batch_mode_fisher_outfile/FL-E13-5_MB-E10-5_chr1_chr1_4487435_4487435_Sox17_H0_rejected.bed,cHi-C/chicDifferentialTest/batch_mode_fisher_outfile/FL-E13-5_MB-E10-5_chr1_chr1_14300280_14300280_Eya1_H0_rejected.bed,cHi-C/chicDifferentialTest/batch_mode_fisher_outfile/FL-E13-5_MB-E10-5_chr1_chr1_19093103_19093103_Tfap2d_H0_rejected.bed"/> | |
| 164 <param name="significantFiles" value="cHi-C/chicSignificantInteractions/output_5/FL-E13-5_chr1_chr1_4487435_4487435_Sox17_output_significant.bed,cHi-C/chicSignificantInteractions/output_5/FL-E13-5_chr1_chr1_14300280_14300280_Eya1_output_significant.bed,cHi-C/chicSignificantInteractions/output_5/FL-E13-5_chr1_chr1_19093103_19093103_Tfap2d_output_significant.bed,cHi-C/chicSignificantInteractions/output_5/MB-E10-5_chr1_chr1_4487435_4487435_Sox17_output_significant.bed,cHi-C/chicSignificantInteractions/output_5/MB-E10-5_chr1_chr1_14300280_14300280_Eya1_output_significant.bed,cHi-C/chicSignificantInteractions/output_5/MB-E10-5_chr1_chr1_19093103_19093103_Tfap2d_output_significant.bed"/> | |
| 165 | |
| 166 | |
| 167 <conditional name="batchmode_conditional"> | |
| 168 <param name="batchmode_selector" value='optionBatchmode'/> | |
| 169 <param name="interactionFilesOrderFile" value='cHi-C/chicViewpoint/fileNames_two_matrices.txt'/> | |
| 170 <param name="differentialFilesOrderFile" value='cHi-C/chicDifferentialTest/rejectedFilesList.txt'/> | |
| 171 <param name="significantInteractionFilesOrderFile" value='cHi-C/chicSignificantInteractions/output_5_significant_files.txt'/> | |
| 172 <param name='computeSampleNumber' value='2'/> | |
| 173 </conditional> | |
| 174 <param name="pvalue" value='True'/> | |
| 175 <param name="alpha" value='0.5'/> | |
| 176 <param name='backgroundModelFile' value='cHi-C/background.bed'/> | |
| 177 <param name='xFold' value='1.5'/> | |
| 178 <output_collection name="plotsCollection" type="list" count="3"> | |
| 179 <element name="Eya1_FL-E13-5_MB-E10-5_chr1_chr1_14300280" file="cHi-C/chicPlotViewpoint/batchMode/two/Eya1_FL-E13-5_MB-E10-5_chr1_chr1_14300280.png" ftype="png" compare='sim_size' delta='40000'/> | |
| 180 <element name="Sox17_FL-E13-5_MB-E10-5_chr1_chr1_4487435" file="cHi-C/chicPlotViewpoint/batchMode/two/Sox17_FL-E13-5_MB-E10-5_chr1_chr1_4487435.png" ftype="png" compare='sim_size' delta='40000'/> | |
| 181 <element name="Tfap2d_FL-E13-5_MB-E10-5_chr1_chr1_19093103" file="cHi-C/chicPlotViewpoint/batchMode/two/Tfap2d_FL-E13-5_MB-E10-5_chr1_chr1_19093103.png" ftype="png" compare='sim_size' delta='40000'/> | |
| 182 </output_collection> | |
| 183 | |
| 184 </test> | |
| 185 <test> | |
| 186 <param name="interactionFiles" value="cHi-C/chicViewpoint/output_1/FL-E13-5_chr1_chr1_14300280_14300280_Eya1.bed"/> | |
| 187 | |
| 188 <conditional name="batchmode_conditional"> | |
| 189 <param name="batchmode_selector" value='optionSinglemode'/> | |
| 190 </conditional> | |
| 191 | |
| 192 <output_collection name="plotsCollection" type="list" count="1"> | |
| 193 <element name="out_plot" file="cHi-C/chicPlotViewpoint/FL-E13-5_chr1_chr1_14300280_14300280_Eya1.png" ftype="png" compare='sim_size' delta='40000'/> | |
| 194 | |
| 195 </output_collection> | |
| 196 </test> | |
| 197 </tests> | |
| 198 <help><![CDATA[ | |
| 199 | |
| 200 Plot of viewpoints | |
| 201 ================== | |
| 202 | |
| 203 chicPlotViewpoint plots one or many viewpoints with the average background model and the computed p-value per sample. Moreover it can highlight differential interactions of two samples and or significant regions. | |
| 204 | |
| 205 An example usage is: | |
| 206 | |
| 207 `$ chicPlotViewpoint --interactionFile viewpoint1.bed viewpoint2.bed --range 500000 500000 --backgroundModelFile background_model.bed --pValue --outFileName viewpoint1_2.png --dpi 300` | |
| 208 | |
| 209 | |
| 210 In batch mode the list of file names and the folders containing the files need to be given: | |
| 211 | |
| 212 `$ chicPlotViewpoint --interactionFile viewpoint_names.txt -interactionFileFolder viewpointFilesFolder --differentialTestResult rejected_H0.txt --differentialTestResultsFolder differentialFolder --range 500000 500000 --backgroundModelFile background_model.bed --pValue --outputFolder plotsFOlder --dpi 300 --threads 20` | |
| 213 | |
| 214 | |
| 215 For more information about HiCExplorer please consider our documentation on readthedocs.io_ | |
| 216 | |
| 217 .. _readthedocs.io: http://hicexplorer.readthedocs.io/en/latest/index.html | |
| 218 ]]></help> | |
| 219 <expand macro="citations" /> | |
| 220 </tool> |
