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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit 2772547f531819d3f6d892ed041fa39b82e3550f
author iuc
date Wed, 05 Jul 2017 11:56:37 -0400
parents 4644c2eee4e5
children
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a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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1 """
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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2 genetrack.py
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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3
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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4 Input: either scidx or gff format of reads
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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5 Output: Called peaks in gff format
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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6 """
4
4644c2eee4e5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit 19ea4feff5ccf3744c549b9a67259947a1cb90ba
iuc
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7 import csv
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a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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8 import optparse
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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9 import os
4
4644c2eee4e5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit 19ea4feff5ccf3744c549b9a67259947a1cb90ba
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10
0
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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11 import genetrack_util
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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12
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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13 CHUNK_SIZE = 10000000
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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15
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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16 if __name__ == '__main__':
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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17 parser = optparse.OptionParser()
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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18 parser.add_option('-t', '--input_format', dest='input_format', type='string', help='Input format')
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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19 parser.add_option('-i', '--input', dest='inputs', type='string', action='append', nargs=2, help='Input datasets')
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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20 parser.add_option('-s', '--sigma', dest='sigma', type='int', default=5, help='Sigma.')
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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21 parser.add_option('-e', '--exclusion', dest='exclusion', type='int', default=20, help='Exclusion zone.')
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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22 parser.add_option('-u', '--up_width', dest='up_width', type='int', default=10, help='Upstream width of called peaks.')
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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23 parser.add_option('-d', '--down_width', dest='down_width', type='int', default=10, help='Downstream width of called peaks.')
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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24 parser.add_option('-f', '--filter', dest='filter', type='int', default=1, help='Absolute read filter.')
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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25 options, args = parser.parse_args()
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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26
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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27 os.mkdir('output')
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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28 for (dataset_path, hid) in options.inputs:
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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29 if options.input_format == 'gff':
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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30 # Make sure the reads for each chromosome are sorted by index.
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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31 input_path = genetrack_util.sort_chromosome_reads_by_index(dataset_path)
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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32 else:
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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33 # We're processing scidx data.
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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34 input_path = dataset_path
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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35 output_name = 's%se%su%sd%sF%s_on_data_%s' % (options.sigma,
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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36 options.exclusion,
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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37 options.up_width,
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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38 options.down_width,
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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39 options.filter,
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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40 hid)
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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41 output_path = os.path.join('output', output_name)
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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42 reader = csv.reader(open(input_path, 'rU'), delimiter='\t')
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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43 writer = csv.writer(open(output_path, 'wt'), delimiter='\t')
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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44 width = options.sigma * 5
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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45 manager = genetrack_util.ChromosomeManager(reader)
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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46 while not manager.done:
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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47 cname = manager.chromosome_name()
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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48 # Should we process this chromosome?
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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49 data = manager.load_chromosome()
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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50 if not data:
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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51 continue
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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52 keys = genetrack_util.make_keys(data)
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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53 lo, hi = genetrack_util.get_range(data)
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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54 for chunk in genetrack_util.get_chunks(lo, hi, size=CHUNK_SIZE, overlap=width):
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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55 (slice_start, slice_end), process_bounds = chunk
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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56 window = genetrack_util.get_window(data, slice_start, slice_end, keys)
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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57 genetrack_util.process_chromosome(cname,
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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58 window,
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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59 writer,
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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60 process_bounds,
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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61 width,
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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62 options.sigma,
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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63 options.up_width,
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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64 options.down_width,
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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65 options.exclusion,
a387a9e49dd6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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66 options.filter)