##fileformat=VCFv4.2
##FORMAT=<ID=AD,Number=R,Type=Integer,Description="Allelic depths for the ref and alt alleles in the order listed">
##FORMAT=<ID=AF,Number=A,Type=Float,Description="Allele fractions of alternate alleles in the tumor">
##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Approximate read depth (reads with MQ=255 or with bad mates are filtered)">
##FORMAT=<ID=F1R2,Number=R,Type=Integer,Description="Count of reads in F1R2 pair orientation supporting each allele">
##FORMAT=<ID=F2R1,Number=R,Type=Integer,Description="Count of reads in F2R1 pair orientation supporting each allele">
##FORMAT=<ID=FAD,Number=R,Type=Integer,Description="Count of fragments supporting each allele.">
##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
##FORMAT=<ID=PGT,Number=1,Type=String,Description="Physical phasing haplotype information, describing how the alternate alleles are phased in relation to one another; will always be heterozygous and is not intended to describe called alleles">
##FORMAT=<ID=PID,Number=1,Type=String,Description="Physical phasing ID information, where each unique ID within a given sample (but not across samples) connects records within a phasing group">
##FORMAT=<ID=PL,Number=G,Type=Integer,Description="Normalized, Phred-scaled likelihoods for genotypes as defined in the VCF specification">
##FORMAT=<ID=PS,Number=1,Type=Integer,Description="Phasing set (typically the position of the first variant in the set)">
##FORMAT=<ID=SB,Number=4,Type=Integer,Description="Per-sample component statistics which comprise the Fisher's Exact Test to detect strand bias.">
##GATKCommandLine=<ID=Mutect2,CommandLine="Mutect2 --f1r2-median-mq 50 --f1r2-min-bq 20 --f1r2-max-depth 200 --tumor-sample SRR8525881 --af-of-alleles-not-in-resource -1.0 --tumor-lod-to-emit 3.0 --initial-tumor-lod 2.0 --pcr-snv-qual 40 --pcr-indel-qual 40 --max-population-af 0.01 --downsampling-stride 1 --normal-lod 2.2 --base-quality-score-threshold 18 --native-pair-hmm-threads 1 --min-base-quality-score 10 --output output.vcf --max-reads-per-alignment-start 50 --interval-set-rule UNION --interval-padding 0 --interval-exclusion-padding 0 --interval-merging-rule ALL --input tumor.bam --read-validation-stringency SILENT --reference reference.fa --add-output-sam-program-record true --add-output-vcf-command-line true --verbosity ERROR --QUIET true --gcs-max-retries 20 --annotation StrandBiasBySample --annotation BaseQualityHistogram --annotation OrientationBiasReadCounts --annotation-group StandardMutectAnnotation --flow-likelihood-parallel-threads 0 --flow-likelihood-optimized-comp false --trim-to-haplotype true --exact-matching false --flow-use-t0-tag false --flow-remove-non-single-base-pair-indels false --flow-remove-one-zero-probs false --flow-quantization-bins 121 --flow-fill-empty-bins-value 0.001 --flow-symmetric-indel-probs false --flow-report-insertion-or-deletion false --flow-disallow-probs-larger-than-call false --flow-lump-probs false --flow-retain-max-n-probs-base-format false --flow-probability-scaling-factor 10 --flow-order-cycle-length 4 --keep-boundary-flows false --genotype-pon-sites false --genotype-germline-sites false --genotype-germline-sites-fraction 1.0 --mitochondria-mode false --permutect-ref-downsample 10 --permutect-alt-downsample 20 --permutect-non-artifact-ratio 1 --permutect-dataset-mode ILLUMINA --base-qual-correction-factor 5 --callable-depth 10 --max-suspicious-reads-per-alignment-start 0 --ignore-itr-artifacts false --gvcf-lod-band -2.5 --gvcf-lod-band -2.0 --gvcf-lod-band -1.5 --gvcf-lod-band -1.0 --gvcf-lod-band -0.5 --gvcf-lod-band 0.0 --gvcf-lod-band 0.5 --gvcf-lod-band 1.0 --minimum-allele-fraction 0.0 --independent-mates false --flow-mode NONE --disable-adaptive-pruning false --kmer-size 10 --kmer-size 25 --dont-increase-kmer-sizes-for-cycles false --allow-non-unique-kmers-in-ref false --num-pruning-samples 1 --min-dangling-branch-length 4 --recover-all-dangling-branches false --max-num-haplotypes-in-population 128 --min-pruning 2 --adaptive-pruning-initial-error-rate 0.001 --pruning-lod-threshold 2.302585092994046 --pruning-seeding-lod-threshold 9.210340371976184 --max-unpruned-variants 100 --linked-de-bruijn-graph false --disable-artificial-haplotype-recovery false --enable-legacy-graph-cycle-detection false --debug-assembly false --debug-graph-transformations false --capture-assembly-failure-bam false --num-matching-bases-in-dangling-end-to-recover -1 --error-correction-log-odds -Infinity --error-correct-reads false --kmer-length-for-read-error-correction 25 --min-observations-for-kmer-to-be-solid 20 --likelihood-calculation-engine PairHMM --dragstr-het-hom-ratio 2 --dont-use-dragstr-pair-hmm-scores false --pair-hmm-gap-continuation-penalty 10 --expected-mismatch-rate-for-read-disqualification 0.02 --pair-hmm-implementation FASTEST_AVAILABLE --pcr-indel-model CONSERVATIVE --phred-scaled-global-read-mismapping-rate 45 --disable-symmetric-hmm-normalizing false --disable-cap-base-qualities-to-map-quality false --enable-dynamic-read-disqualification-for-genotyping false --dynamic-read-disqualification-threshold 1.0 --native-pair-hmm-use-double-precision false --flow-hmm-engine-min-indel-adjust 6 --flow-hmm-engine-flat-insertion-penatly 45 --flow-hmm-engine-flat-deletion-penatly 45 --pileup-detection false --use-pdhmm false --use-pdhmm-overlap-optimization false --make-determined-haps-from-pd-code false --print-pileupcalling-status false --fallback-gga-if-pdhmm-fails true --pileup-detection-enable-indel-pileup-calling false --pileup-detection-active-region-phred-threshold 0.0 --num-artificial-haplotypes-to-add-per-allele 5 --artifical-haplotype-filtering-kmer-size 10 --pileup-detection-snp-alt-threshold 0.1 --pileup-detection-indel-alt-threshold 0.1 --pileup-detection-absolute-alt-depth 0.0 --pileup-detection-snp-adjacent-to-assembled-indel-range 5 --pileup-detection-snp-basequality-filter 12 --pileup-detection-bad-read-tolerance 0.0 --pileup-detection-proper-pair-read-badness true --pileup-detection-edit-distance-read-badness-threshold 0.08 --pileup-detection-chimeric-read-badness true --pileup-detection-template-mean-badness-threshold 0.0 --pileup-detection-template-std-badness-threshold 0.0 --pileup-detection-filter-assembly-alt-bad-read-tolerance 0.0 --pileup-detection-edit-distance-read-badness-for-assembly-filtering-threshold 0.12 --bam-writer-type CALLED_HAPLOTYPES --dont-use-soft-clipped-bases false --override-fragment-softclip-check false --smith-waterman FASTEST_AVAILABLE --emit-ref-confidence NONE --max-mnp-distance 1 --force-call-filtered-alleles false --reference-model-deletion-quality 30 --soft-clip-low-quality-ends false --allele-informative-reads-overlap-margin 2 --smith-waterman-dangling-end-match-value 25 --smith-waterman-dangling-end-mismatch-penalty -50 --smith-waterman-dangling-end-gap-open-penalty -110 --smith-waterman-dangling-end-gap-extend-penalty -6 --smith-waterman-haplotype-to-reference-match-value 200 --smith-waterman-haplotype-to-reference-mismatch-penalty -150 --smith-waterman-haplotype-to-reference-gap-open-penalty -260 --smith-waterman-haplotype-to-reference-gap-extend-penalty -11 --smith-waterman-read-to-haplotype-match-value 10 --smith-waterman-read-to-haplotype-mismatch-penalty -15 --smith-waterman-read-to-haplotype-gap-open-penalty -30 --smith-waterman-read-to-haplotype-gap-extend-penalty -5 --flow-assembly-collapse-hmer-size 0 --flow-assembly-collapse-partial-mode false --flow-filter-alleles false --flow-filter-alleles-qual-threshold 30.0 --flow-filter-alleles-sor-threshold 3.0 --flow-filter-lone-alleles false --flow-filter-alleles-debug-graphs false --min-assembly-region-size 50 --max-assembly-region-size 300 --active-probability-threshold 0.002 --max-prob-propagation-distance 50 --force-active false --assembly-region-padding 100 --padding-around-indels 75 --padding-around-snps 20 --padding-around-strs 75 --max-extension-into-assembly-region-padding-legacy 25 --enable-legacy-assembly-region-trimming false --seconds-between-progress-updates 10.0 --disable-sequence-dictionary-validation false --create-output-bam-index true --create-output-bam-md5 false --create-output-variant-index true --create-output-variant-md5 false --max-variants-per-shard 0 --lenient false --cloud-prefetch-buffer 40 --cloud-index-prefetch-buffer -1 --disable-bam-index-caching false --sites-only-vcf-output false --help false --version false --showHidden false --use-jdk-deflater false --use-jdk-inflater false --gcs-project-for-requester-pays  --disable-tool-default-read-filters false --max-read-length 2147483647 --min-read-length 30 --minimum-mapping-quality 20 --disable-tool-default-annotations false --enable-all-annotations false",Version="4.6.2.0",Date="February 2, 2026, 12:29:39?AM GMT">
##INFO=<ID=AS_SB_TABLE,Number=1,Type=String,Description="Allele-specific forward/reverse read counts for strand bias tests. Includes the reference and alleles separated by |.">
##INFO=<ID=AS_UNIQ_ALT_READ_COUNT,Number=A,Type=Integer,Description="Number of reads with unique start and mate end positions for each alt at a variant site">
##INFO=<ID=BQHIST,Number=A,Type=Integer,Description="Base quality counts for each allele represented sparsely as alternating entries of qualities and counts for each allele.For example [10,1,0,20,0,1] means one ref base with quality 10 and one alt base with quality 20.">
##INFO=<ID=CONTQ,Number=1,Type=Float,Description="Phred-scaled qualities that alt allele are not due to contamination">
##INFO=<ID=DP,Number=1,Type=Integer,Description="Approximate read depth; some reads may have been filtered">
##INFO=<ID=ECNT,Number=1,Type=Integer,Description="Number of potential somatic events in the assembly region">
##INFO=<ID=ECNTH,Number=A,Type=Integer,Description="Number of somatic events in best supporting haplotype for each alt allele">
##INFO=<ID=GERMQ,Number=1,Type=Integer,Description="Phred-scaled quality that alt alleles are not germline variants">
##INFO=<ID=MBQ,Number=R,Type=Integer,Description="median base quality by allele">
##INFO=<ID=MFRL,Number=R,Type=Integer,Description="median fragment length by allele">
##INFO=<ID=MMQ,Number=R,Type=Integer,Description="median mapping quality by allele">
##INFO=<ID=MPOS,Number=A,Type=Integer,Description="median distance from end of read">
##INFO=<ID=NALOD,Number=A,Type=Float,Description="Log 10 odds of artifact in normal with same allele fraction as tumor">
##INFO=<ID=NCount,Number=1,Type=Integer,Description="Count of N bases in the pileup">
##INFO=<ID=NLOD,Number=A,Type=Float,Description="Normal log 10 likelihood ratio of diploid het or hom alt genotypes">
##INFO=<ID=OCM,Number=1,Type=Integer,Description="Number of alt reads whose original alignment doesn't match the current contig.">
##INFO=<ID=PON,Number=0,Type=Flag,Description="site found in panel of normals">
##INFO=<ID=POPAF,Number=A,Type=Float,Description="negative log 10 population allele frequencies of alt alleles">
##INFO=<ID=ROQ,Number=1,Type=Float,Description="Phred-scaled qualities that alt allele are not due to read orientation artifact">
##INFO=<ID=RPA,Number=R,Type=Integer,Description="Number of times tandem repeat unit is repeated, for each allele (including reference)">
##INFO=<ID=RU,Number=1,Type=String,Description="Tandem repeat unit (bases)">
##INFO=<ID=SEQQ,Number=1,Type=Integer,Description="Phred-scaled quality that alt alleles are not sequencing errors">
##INFO=<ID=STR,Number=0,Type=Flag,Description="Variant is a short tandem repeat">
##INFO=<ID=STRANDQ,Number=1,Type=Integer,Description="Phred-scaled quality of strand bias artifact">
##INFO=<ID=STRQ,Number=1,Type=Integer,Description="Phred-scaled quality that alt alleles in STRs are not polymerase slippage errors">
##INFO=<ID=TLOD,Number=A,Type=Float,Description="Log 10 likelihood ratio score of variant existing versus not existing">
##MutectVersion=2.2
##contig=<ID=K03455,length=9719>
##filtering_status=Warning: unfiltered Mutect 2 calls.  Please run FilterMutectCalls to remove false positives.
##source=Mutect2
##tumor_sample=SRR8525881
#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	SRR8525881
K03455	2644	.	A	G	.	.	AS_SB_TABLE=41,9|2,0;BQHIST=20,18,0,32,1,0,37,12,0,38,4,1,39,15,1;DP=61;ECNT=6;ECNTH=6;MBQ=37,39;MFRL=242,292;MMQ=60,60;MPOS=25;POPAF=7.30;TLOD=3.99	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:50,2:0.064:52:21,0:21,2:42,2:41,9,2,0
K03455	2652	.	G	A	.	.	AS_SB_TABLE=0,0|122,15;BQHIST=16,1,0,18,9,0,20,27,0,29,5,0,32,21,0,33,13,0,34,2,0,37,27,0,38,16,0,39,14,0;DP=137;ECNT=6;ECNTH=5;MBQ=0,33;MFRL=0,266;MMQ=60,60;MPOS=5;POPAF=7.30;TLOD=614.56	GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB	0|1:0,137:0.992:137:0,53:0,59:0,124:0|1:2652_G_A:2652:0,0,122,15
K03455	2660	.	T	C	.	.	AS_SB_TABLE=0,0|152,18;BQHIST=15,1,0,16,1,0,17,3,0,20,27,0,29,1,0,32,4,0,33,3,0,34,2,0,35,6,0,37,50,0,38,27,0,39,22,0;DP=170;ECNT=6;ECNTH=5;MBQ=0,37;MFRL=0,269;MMQ=60,60;MPOS=13;POPAF=7.30;TLOD=762.96	GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB	0|1:0,170:0.994:170:0,63:0,68:0,154:0|1:2652_G_A:2652:0,0,152,18
K03455	2664	.	A	G	.	.	AS_SB_TABLE=0,0|155,18;BQHIST=18,0,2,19,0,3,20,0,30,30,0,3,32,0,15,33,0,13,34,0,2,35,0,3,36,0,2,37,0,19,38,0,67,39,0,12;DP=173;ECNT=6;ECNTH=5;MBQ=0,37;MFRL=0,267;MMQ=60,60;MPOS=14;POPAF=7.30;TLOD=774.91	GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB	0|1:0,173:0.994:173:0,76:0,76:0,157:0|1:2652_G_A:2652:0,0,155,18
K03455	2669	.	G	A	.	.	AS_SB_TABLE=0,0|162,20;BQHIST=16,2,0,18,1,0,19,2,0,20,35,0,29,1,0,32,5,0,33,9,0,34,3,0,36,1,0,37,47,0,38,44,0,39,32,0;DP=185;ECNT=6;ECNTH=5;MBQ=0,37;MFRL=0,271;MMQ=60,60;MPOS=18;POPAF=7.30;TLOD=800.27	GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB	0|1:0,182:0.994:182:0,78:0,82:0,164:0|1:2652_G_A:2652:0,0,162,20
K03455	2720	.	T	C	.	.	AS_SB_TABLE=0,0|254,46;BQHIST=14,3,0,16,12,0,17,1,0,18,24,0,20,65,0,30,1,0,32,3,0,33,25,0,34,8,0,36,14,0,37,81,0,38,24,0,39,39,0;DP=348;ECNT=6;ECNTH=5;MBQ=0,36;MFRL=0,259;MMQ=60,60;MPOS=51;POPAF=7.30;TLOD=1043.80	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:0,300:0.996:300:0,106:0,121:0,261:0,0,254,46
K03455	2797	.	G	A	.	.	AS_SB_TABLE=0,0|315,161;BQHIST=15,1,0,16,2,0,17,1,0,18,19,0,20,182,0,32,5,0,33,19,0,35,8,0,36,9,0,37,48,0,38,60,0,39,119,0;DP=504;ECNT=9;ECNTH=7;MBQ=0,35;MFRL=0,242;MMQ=60,60;MPOS=45;POPAF=7.30;TLOD=1635.75	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:0,476:0.998:476:0,185:0,168:0,395:0,0,315,161
K03455	2831	.	A	T	.	.	AS_SB_TABLE=315,204|0,18;BQHIST=14,0,4,15,0,13,16,1,2,17,3,50,18,11,14,19,0,3,20,0,148,29,0,3,30,1,5,31,0,6,32,0,8,33,2,42,34,0,15,36,0,6,37,0,53,38,0,45,39,0,100;DP=558;ECNT=9;ECNTH=8;MBQ=33,18;MFRL=238,273;MMQ=60,60;MPOS=24;POPAF=7.30;TLOD=4.77	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:519,18:0.024:537:182,0:156,0:432,12:315,204,0,18
K03455	2848	.	T	TA	.	.	AS_SB_TABLE=14,6|318,254;BQHIST=14,2,0,15,21,2,16,10,0,17,1,0,19,2,0,20,260,9,27,1,0,29,1,0,30,11,2,31,1,0,32,15,0,33,18,3,34,12,0,35,1,0,36,19,1,37,46,1,38,68,0,39,81,2;DP=621;ECNT=9;ECNTH=7;MBQ=20,20;MFRL=270,245;MMQ=60,60;MPOS=32;POPAF=7.30;RPA=6,7;RU=A;STR;TLOD=1153.15	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:20,572:0.982:592:7,179:6,177:14,447:14,6,318,254
K03455	2872	.	T	A	.	.	AS_SB_TABLE=267,299|34,0;BQHIST=14,2,1,15,2,0,16,0,12,17,10,5,18,19,51,19,0,35,20,0,173,27,1,1,29,0,16,30,0,1,31,0,7,32,0,24,33,0,31,34,0,12,35,0,10,36,0,10,37,0,79,38,0,43,39,0,54;DP=639;ECNT=9;ECNTH=7;MBQ=29,18;MFRL=240,276;MMQ=60,60;MPOS=29;POPAF=7.30;TLOD=9.50	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:566,34:0.031:600:135,1:134,0:468,21:267,299,34,0
K03455	2874	.	C	T	.	.	AS_SB_TABLE=0,1|311,300;BQHIST=16,8,0,17,7,0,18,52,0,19,12,0,20,201,0,29,4,0,30,3,0,31,9,0,32,34,0,33,42,0,34,14,0,35,13,0,36,10,0,37,90,1,38,53,0,39,52,0;DP=615;ECNT=9;ECNTH=7;MBQ=37,32;MFRL=235,242;MMQ=60,60;MPOS=32;POPAF=7.30;TLOD=2682.59	GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB	0|1:1,611:0.996:612:1,174:0,160:1,505:0|1:2874_C_T:2874:0,1,311,300
K03455	2882	.	G	T	.	.	AS_SB_TABLE=4,2|290,266;BQHIST=14,1,0,16,5,1,17,3,2,18,22,0,19,11,0,20,200,0,29,1,1,30,4,0,31,4,1,32,5,0,33,24,0,34,1,0,35,19,0,36,9,0,37,87,0,38,53,0,39,106,0;DP=581;ECNT=9;ECNTH=7;MBQ=17,33;MFRL=276,247;MMQ=60,60;MPOS=41;POPAF=7.30;TLOD=1871.45	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:6,556:0.994:562:1,167:0,157:4,457:4,2,290,266
K03455	2894	.	T	C	.	.	AS_SB_TABLE=0,0|294,271;BQHIST=14,1,0,15,6,0,16,4,0,17,13,0,18,32,0,19,4,0,20,167,0,27,1,0,29,2,0,30,2,0,31,2,0,32,9,0,33,27,0,34,10,0,35,6,0,36,8,0,37,81,0,38,65,0,39,116,0;DP=565;ECNT=9;ECNTH=7;MBQ=0,34;MFRL=0,250;MMQ=60,60;MPOS=42;POPAF=7.30;TLOD=2595.31	GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB	0|1:0,565:0.998:565:0,146:0,153:0,476:0|1:2874_C_T:2874:0,0,294,271
K03455	2906	.	C	T	.	.	AS_SB_TABLE=0,0|249,259;BQHIST=16,2,0,18,4,0,19,10,0,20,155,0,30,1,0,31,2,0,32,4,0,33,18,0,34,3,0,35,17,0,36,10,0,37,62,0,38,79,0,39,141,0;DP=508;ECNT=9;ECNTH=7;MBQ=0,37;MFRL=0,251;MMQ=60,60;MPOS=42;POPAF=7.30;TLOD=2422.86	GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB	0|1:0,508:0.998:508:0,132:0,159:0,440:0|1:2874_C_T:2874:0,0,249,259
K03455	2913	.	G	A	.	.	AS_SB_TABLE=0,0|248,257;BQHIST=17,1,0,18,5,0,19,5,0,20,140,0,29,1,0,30,2,0,31,1,0,32,2,0,33,15,0,34,1,0,35,4,0,36,3,0,37,62,0,38,71,0,39,180,0,40,4,0;DP=505;ECNT=9;ECNTH=7;MBQ=0,38;MFRL=0,251;MMQ=60,60;MPOS=42;POPAF=7.30;TLOD=2413.86	GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB	0|1:0,505:0.998:505:0,133:0,157:0,438:0|1:2874_C_T:2874:0,0,248,257
K03455	2987	.	C	T	.	.	AS_SB_TABLE=0,0|76,130;BQHIST=16,4,0,17,4,0,19,5,0,20,15,0,30,1,0,31,6,0,32,8,0,33,30,0,34,8,0,35,9,0,36,1,0,37,46,0,38,31,0,39,38,0;DP=211;ECNT=5;ECNTH=3;MBQ=0,37;MFRL=0,270;MMQ=60,60;MPOS=23;POPAF=7.30;TLOD=782.35	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:0,206:0.995:206:0,85:0,96:0,199:0,0,76,130
K03455	3016	.	C	A	.	.	AS_SB_TABLE=57,28|6,0;BQHIST=14,0,2,15,0,1,18,5,24,20,0,5,29,0,1,30,0,2,32,0,4,33,1,10,34,0,2,35,0,2,36,0,8,37,0,5,38,0,15,39,0,4;DP=95;ECNT=5;ECNTH=3;MBQ=33,18;MFRL=278,272;MMQ=60,60;MPOS=6;POPAF=7.30;TLOD=4.56	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:85,6:0.073:91:23,1:31,0:83,6:57,28,6,0
K03455	3020	.	A	G	.	.	AS_SB_TABLE=0,0|59,26;BQHIST=14,0,12,16,0,1,18,0,1,20,0,4,27,0,2,29,0,1,30,0,7,32,0,8,33,0,2,34,0,1,36,0,19,37,0,4,38,0,22,39,0,1;DP=91;ECNT=5;ECNTH=3;MBQ=0,36;MFRL=0,289;MMQ=60,60;MPOS=11;POPAF=7.30;TLOD=292.32	GT:AD:AF:DP:F1R2:F2R1:FAD:SB	0/1:0,85:0.988:85:0,30:0,37:0,83:0,0,59,26
K03455	3036	.	A	G	.	.	AS_SB_TABLE=16,3|3,0;BQHIST=15,1,0,19,7,2,29,1,0,30,1,0,33,3,0,37,4,1,38,2,0;DP=36;ECNT=5;ECNTH=4;MBQ=30,19;MFRL=289,298;MMQ=60,60;MPOS=0;POPAF=7.30;TLOD=4.59	GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB	0|1:19,3:0.160:22:7,1:3,0:19,3:0|1:3036_A_G:3036:16,3,3,0
K03455	3038	.	C	G	.	.	AS_SB_TABLE=16,3|3,0;BQHIST=14,0,1,19,5,0,30,1,0,32,1,0,33,1,0,35,1,0,37,1,0,38,2,0,39,3,0;DP=22;ECNT=5;ECNTH=4;MBQ=33,14;MFRL=289,298;MMQ=60,60;MPOS=8;POPAF=7.30;TLOD=4.70	GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB	0|1:19,3:0.166:22:5,0:4,0:19,3:0|1:3036_A_G:3036:16,3,3,0