Mercurial > repos > iuc > gatk4_mutect2
diff test-data/Mutect2-out5.vcf @ 8:55e8a08e39e1 draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/gatk4 commit 30797e29f13902f17652cf35585e52b363c28a31
| author | iuc |
|---|---|
| date | Tue, 03 Feb 2026 18:50:29 +0000 |
| parents | d2c0c2698f58 |
| children |
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--- a/test-data/Mutect2-out5.vcf Wed Aug 24 17:58:18 2022 +0000 +++ b/test-data/Mutect2-out5.vcf Tue Feb 03 18:50:29 2026 +0000 @@ -4,6 +4,7 @@ ##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Approximate read depth (reads with MQ=255 or with bad mates are filtered)"> ##FORMAT=<ID=F1R2,Number=R,Type=Integer,Description="Count of reads in F1R2 pair orientation supporting each allele"> ##FORMAT=<ID=F2R1,Number=R,Type=Integer,Description="Count of reads in F2R1 pair orientation supporting each allele"> +##FORMAT=<ID=FAD,Number=R,Type=Integer,Description="Count of fragments supporting each allele."> ##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality"> ##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype"> ##FORMAT=<ID=PGT,Number=1,Type=String,Description="Physical phasing haplotype information, describing how the alternate alleles are phased in relation to one another; will always be heterozygous and is not intended to describe called alleles"> @@ -11,18 +12,19 @@ ##FORMAT=<ID=PL,Number=G,Type=Integer,Description="Normalized, Phred-scaled likelihoods for genotypes as defined in the VCF specification"> ##FORMAT=<ID=PS,Number=1,Type=Integer,Description="Phasing set (typically the position of the first variant in the set)"> ##FORMAT=<ID=SB,Number=4,Type=Integer,Description="Per-sample component statistics which comprise the Fisher's Exact Test to detect strand bias."> -##GATKCommandLine=<ID=Mutect2,CommandLine="Mutect2 --tumor-sample SRR8525881 --bam-output debug.bam --output output.vcf --assembly-region-out assembly-region.tab --input tumor.bam --reference reference.fa --QUIET true --f1r2-median-mq 50 --f1r2-min-bq 20 --f1r2-max-depth 200 --genotype-pon-sites false --genotype-germline-sites false --af-of-alleles-not-in-resource -1.0 --mitochondria-mode false --tumor-lod-to-emit 3.0 --initial-tumor-lod 2.0 --pcr-snv-qual 40 --pcr-indel-qual 40 --max-population-af 0.01 --downsampling-stride 1 --callable-depth 10 --max-suspicious-reads-per-alignment-start 0 --normal-lod 2.2 --ignore-itr-artifacts false --gvcf-lod-band -2.5 --gvcf-lod-band -2.0 --gvcf-lod-band -1.5 --gvcf-lod-band -1.0 --gvcf-lod-band -0.5 --gvcf-lod-band 0.0 --gvcf-lod-band 0.5 --gvcf-lod-band 1.0 --minimum-allele-fraction 0.0 --independent-mates false --disable-adaptive-pruning false --kmer-size 10 --kmer-size 25 --dont-increase-kmer-sizes-for-cycles false --allow-non-unique-kmers-in-ref false --num-pruning-samples 1 --min-dangling-branch-length 4 --recover-all-dangling-branches false --max-num-haplotypes-in-population 128 --min-pruning 2 --adaptive-pruning-initial-error-rate 0.001 --pruning-lod-threshold 2.302585092994046 --max-unpruned-variants 100 --linked-de-bruijn-graph false --disable-artificial-haplotype-recovery false --debug-assembly false --debug-graph-transformations false --capture-assembly-failure-bam false --error-correction-log-odds -Infinity --error-correct-reads false --kmer-length-for-read-error-correction 25 --min-observations-for-kmer-to-be-solid 20 --base-quality-score-threshold 18 --pair-hmm-gap-continuation-penalty 10 --pair-hmm-implementation FASTEST_AVAILABLE --pcr-indel-model CONSERVATIVE --phred-scaled-global-read-mismapping-rate 45 --native-pair-hmm-threads 4 --native-pair-hmm-use-double-precision false --bam-writer-type CALLED_HAPLOTYPES --dont-use-soft-clipped-bases false --min-base-quality-score 10 --smith-waterman JAVA --emit-ref-confidence NONE --max-mnp-distance 1 --force-call-filtered-alleles false --allele-informative-reads-overlap-margin 2 --min-assembly-region-size 50 --max-assembly-region-size 300 --active-probability-threshold 0.002 --max-prob-propagation-distance 50 --force-active false --assembly-region-padding 100 --padding-around-indels 75 --padding-around-snps 20 --padding-around-strs 75 --max-reads-per-alignment-start 50 --interval-set-rule UNION --interval-padding 0 --interval-exclusion-padding 0 --interval-merging-rule ALL --read-validation-stringency SILENT --seconds-between-progress-updates 10.0 --disable-sequence-dictionary-validation false --create-output-bam-index true --create-output-bam-md5 false --create-output-variant-index true --create-output-variant-md5 false --lenient false --add-output-sam-program-record true --add-output-vcf-command-line true --cloud-prefetch-buffer 40 --cloud-index-prefetch-buffer -1 --disable-bam-index-caching false --sites-only-vcf-output false --help false --version false --showHidden false --verbosity INFO --use-jdk-deflater false --use-jdk-inflater false --gcs-max-retries 20 --gcs-project-for-requester-pays --disable-tool-default-read-filters false --max-read-length 2147483647 --min-read-length 30 --minimum-mapping-quality 20 --disable-tool-default-annotations false --enable-all-annotations false",Version="4.1.7.0",Date="May 8, 2020 3:33:33 PM CEST"> +##GATKCommandLine=<ID=Mutect2,CommandLine="Mutect2 --tumor-sample SRR8525881 --bam-output debug.bam --output output.vcf --assembly-region-out assembly-region.tab --input tumor.bam --reference reference.fa --QUIET true --f1r2-median-mq 50 --f1r2-min-bq 20 --f1r2-max-depth 200 --flow-likelihood-parallel-threads 0 --flow-likelihood-optimized-comp false --trim-to-haplotype true --exact-matching false --flow-use-t0-tag false --flow-remove-non-single-base-pair-indels false --flow-remove-one-zero-probs false --flow-quantization-bins 121 --flow-fill-empty-bins-value 0.001 --flow-symmetric-indel-probs false --flow-report-insertion-or-deletion false --flow-disallow-probs-larger-than-call false --flow-lump-probs false --flow-retain-max-n-probs-base-format false --flow-probability-scaling-factor 10 --flow-order-cycle-length 4 --keep-boundary-flows false --genotype-pon-sites false --genotype-germline-sites false --genotype-germline-sites-fraction 1.0 --af-of-alleles-not-in-resource -1.0 --mitochondria-mode false --permutect-ref-downsample 10 --permutect-alt-downsample 20 --permutect-non-artifact-ratio 1 --permutect-dataset-mode ILLUMINA --tumor-lod-to-emit 3.0 --initial-tumor-lod 2.0 --pcr-snv-qual 40 --pcr-indel-qual 40 --base-qual-correction-factor 5 --max-population-af 0.01 --downsampling-stride 1 --callable-depth 10 --max-suspicious-reads-per-alignment-start 0 --normal-lod 2.2 --ignore-itr-artifacts false --gvcf-lod-band -2.5 --gvcf-lod-band -2.0 --gvcf-lod-band -1.5 --gvcf-lod-band -1.0 --gvcf-lod-band -0.5 --gvcf-lod-band 0.0 --gvcf-lod-band 0.5 --gvcf-lod-band 1.0 --minimum-allele-fraction 0.0 --independent-mates false --flow-mode NONE --disable-adaptive-pruning false --kmer-size 10 --kmer-size 25 --dont-increase-kmer-sizes-for-cycles false --allow-non-unique-kmers-in-ref false --num-pruning-samples 1 --min-dangling-branch-length 4 --recover-all-dangling-branches false --max-num-haplotypes-in-population 128 --min-pruning 2 --adaptive-pruning-initial-error-rate 0.001 --pruning-lod-threshold 2.302585092994046 --pruning-seeding-lod-threshold 9.210340371976184 --max-unpruned-variants 100 --linked-de-bruijn-graph false --disable-artificial-haplotype-recovery false --enable-legacy-graph-cycle-detection false --debug-assembly false --debug-graph-transformations false --capture-assembly-failure-bam false --num-matching-bases-in-dangling-end-to-recover -1 --error-correction-log-odds -Infinity --error-correct-reads false --kmer-length-for-read-error-correction 25 --min-observations-for-kmer-to-be-solid 20 --likelihood-calculation-engine PairHMM --base-quality-score-threshold 18 --dragstr-het-hom-ratio 2 --dont-use-dragstr-pair-hmm-scores false --pair-hmm-gap-continuation-penalty 10 --expected-mismatch-rate-for-read-disqualification 0.02 --pair-hmm-implementation FASTEST_AVAILABLE --pcr-indel-model CONSERVATIVE --phred-scaled-global-read-mismapping-rate 45 --disable-symmetric-hmm-normalizing false --disable-cap-base-qualities-to-map-quality false --enable-dynamic-read-disqualification-for-genotyping false --dynamic-read-disqualification-threshold 1.0 --native-pair-hmm-threads 4 --native-pair-hmm-use-double-precision false --flow-hmm-engine-min-indel-adjust 6 --flow-hmm-engine-flat-insertion-penatly 45 --flow-hmm-engine-flat-deletion-penatly 45 --pileup-detection false --use-pdhmm false --use-pdhmm-overlap-optimization false --make-determined-haps-from-pd-code false --print-pileupcalling-status false --fallback-gga-if-pdhmm-fails true --pileup-detection-enable-indel-pileup-calling false --pileup-detection-active-region-phred-threshold 0.0 --num-artificial-haplotypes-to-add-per-allele 5 --artifical-haplotype-filtering-kmer-size 10 --pileup-detection-snp-alt-threshold 0.1 --pileup-detection-indel-alt-threshold 0.1 --pileup-detection-absolute-alt-depth 0.0 --pileup-detection-snp-adjacent-to-assembled-indel-range 5 --pileup-detection-snp-basequality-filter 12 --pileup-detection-bad-read-tolerance 0.0 --pileup-detection-proper-pair-read-badness true --pileup-detection-edit-distance-read-badness-threshold 0.08 --pileup-detection-chimeric-read-badness true --pileup-detection-template-mean-badness-threshold 0.0 --pileup-detection-template-std-badness-threshold 0.0 --pileup-detection-filter-assembly-alt-bad-read-tolerance 0.0 --pileup-detection-edit-distance-read-badness-for-assembly-filtering-threshold 0.12 --bam-writer-type CALLED_HAPLOTYPES --dont-use-soft-clipped-bases false --override-fragment-softclip-check false --min-base-quality-score 10 --smith-waterman FASTEST_AVAILABLE --emit-ref-confidence NONE --max-mnp-distance 1 --force-call-filtered-alleles false --reference-model-deletion-quality 30 --soft-clip-low-quality-ends false --allele-informative-reads-overlap-margin 2 --smith-waterman-dangling-end-match-value 25 --smith-waterman-dangling-end-mismatch-penalty -50 --smith-waterman-dangling-end-gap-open-penalty -110 --smith-waterman-dangling-end-gap-extend-penalty -6 --smith-waterman-haplotype-to-reference-match-value 200 --smith-waterman-haplotype-to-reference-mismatch-penalty -150 --smith-waterman-haplotype-to-reference-gap-open-penalty -260 --smith-waterman-haplotype-to-reference-gap-extend-penalty -11 --smith-waterman-read-to-haplotype-match-value 10 --smith-waterman-read-to-haplotype-mismatch-penalty -15 --smith-waterman-read-to-haplotype-gap-open-penalty -30 --smith-waterman-read-to-haplotype-gap-extend-penalty -5 --flow-assembly-collapse-hmer-size 0 --flow-assembly-collapse-partial-mode false --flow-filter-alleles false --flow-filter-alleles-qual-threshold 30.0 --flow-filter-alleles-sor-threshold 3.0 --flow-filter-lone-alleles false --flow-filter-alleles-debug-graphs false --min-assembly-region-size 50 --max-assembly-region-size 300 --active-probability-threshold 0.002 --max-prob-propagation-distance 50 --force-active false --assembly-region-padding 100 --padding-around-indels 75 --padding-around-snps 20 --padding-around-strs 75 --max-extension-into-assembly-region-padding-legacy 25 --max-reads-per-alignment-start 50 --enable-legacy-assembly-region-trimming false --interval-set-rule UNION --interval-padding 0 --interval-exclusion-padding 0 --interval-merging-rule ALL --read-validation-stringency SILENT --seconds-between-progress-updates 10.0 --disable-sequence-dictionary-validation false --create-output-bam-index true --create-output-bam-md5 false --create-output-variant-index true --create-output-variant-md5 false --max-variants-per-shard 0 --lenient false --add-output-sam-program-record true --add-output-vcf-command-line true --cloud-prefetch-buffer 40 --cloud-index-prefetch-buffer -1 --disable-bam-index-caching false --sites-only-vcf-output false --help false --version false --showHidden false --verbosity INFO --use-jdk-deflater false --use-jdk-inflater false --gcs-max-retries 20 --gcs-project-for-requester-pays --disable-tool-default-read-filters false --max-read-length 2147483647 --min-read-length 30 --minimum-mapping-quality 20 --disable-tool-default-annotations false --enable-all-annotations false",Version="4.6.2.0",Date="February 2, 2026, 12:30:15?AM GMT"> ##INFO=<ID=AS_SB_TABLE,Number=1,Type=String,Description="Allele-specific forward/reverse read counts for strand bias tests. Includes the reference and alleles separated by |."> ##INFO=<ID=AS_UNIQ_ALT_READ_COUNT,Number=A,Type=Integer,Description="Number of reads with unique start and mate end positions for each alt at a variant site"> ##INFO=<ID=CONTQ,Number=1,Type=Float,Description="Phred-scaled qualities that alt allele are not due to contamination"> ##INFO=<ID=DP,Number=1,Type=Integer,Description="Approximate read depth; some reads may have been filtered"> -##INFO=<ID=ECNT,Number=1,Type=Integer,Description="Number of events in this haplotype"> +##INFO=<ID=ECNT,Number=1,Type=Integer,Description="Number of potential somatic events in the assembly region"> +##INFO=<ID=ECNTH,Number=A,Type=Integer,Description="Number of somatic events in best supporting haplotype for each alt allele"> ##INFO=<ID=GERMQ,Number=1,Type=Integer,Description="Phred-scaled quality that alt alleles are not germline variants"> -##INFO=<ID=MBQ,Number=R,Type=Integer,Description="median base quality"> -##INFO=<ID=MFRL,Number=R,Type=Integer,Description="median fragment length"> -##INFO=<ID=MMQ,Number=R,Type=Integer,Description="median mapping quality"> +##INFO=<ID=MBQ,Number=R,Type=Integer,Description="median base quality by allele"> +##INFO=<ID=MFRL,Number=R,Type=Integer,Description="median fragment length by allele"> +##INFO=<ID=MMQ,Number=R,Type=Integer,Description="median mapping quality by allele"> ##INFO=<ID=MPOS,Number=A,Type=Integer,Description="median distance from end of read"> -##INFO=<ID=NALOD,Number=A,Type=Float,Description="Negative log 10 odds of artifact in normal with same allele fraction as tumor"> +##INFO=<ID=NALOD,Number=A,Type=Float,Description="Log 10 odds of artifact in normal with same allele fraction as tumor"> ##INFO=<ID=NCount,Number=1,Type=Integer,Description="Count of N bases in the pileup"> ##INFO=<ID=NLOD,Number=A,Type=Float,Description="Normal log 10 likelihood ratio of diploid het or hom alt genotypes"> ##INFO=<ID=OCM,Number=1,Type=Integer,Description="Number of alt reads whose original alignment doesn't match the current contig."> @@ -42,53 +44,54 @@ ##source=Mutect2 ##tumor_sample=SRR8525881 #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT SRR8525881 -K03455 2097 . G A . . AS_SB_TABLE=0,0|10,5;DP=15;ECNT=41;MBQ=0,20;MFRL=0,159;MMQ=60,60;MPOS=34;POPAF=7.30;TLOD=79.89 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:0,15:0.929:15:0,7:0,6:0,0,10,5 -K03455 2106 . T C . . AS_SB_TABLE=9,4|2,2;DP=17;ECNT=41;MBQ=20,30;MFRL=159,90;MMQ=60,60;MPOS=24;POPAF=7.30;TLOD=12.26 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:13,4:0.266:17:6,1:7,1:9,4,2,2 -K03455 2110 . TA CA,CT . . AS_SB_TABLE=0,0|9,5|3,1;DP=18;ECNT=41;MBQ=0,20,35;MFRL=0,159,161;MMQ=60,60,60;MPOS=44,2;POPAF=7.30,7.30;TLOD=60.04,10.69 GT:AD:AF:DP:F1R2:F2R1:SB 0/1/2:0,14,4:0.754,0.187:18:0,6,1:0,8,3:0,0,12,6 -K03455 2118 . A G . . AS_SB_TABLE=3,2|8,3;DP=18;ECNT=41;MBQ=32,20;MFRL=90,160;MMQ=60,60;MPOS=55;POPAF=7.30;TLOD=35.11 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:5,11:0.715:16:1,4:4,6:3,2,8,3 -K03455 2130 . T C . . AS_SB_TABLE=9,5|3,1;DP=18;ECNT=41;MBQ=20,37;MFRL=159,161;MMQ=60,60;MPOS=22;POPAF=7.30;TLOD=10.45 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:14,4:0.199:18:4,1:9,3:9,5,3,1 -K03455 2136 . T C . . AS_SB_TABLE=9,5|3,1;DP=18;ECNT=41;MBQ=20,38;MFRL=159,161;MMQ=60,60;MPOS=28;POPAF=7.30;TLOD=10.45 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:14,4:0.199:18:4,1:10,2:9,5,3,1 -K03455 2155 . A T . . AS_SB_TABLE=3,2|11,4;DP=20;ECNT=41;MBQ=39,20;MFRL=90,233;MMQ=60,60;MPOS=56;POPAF=7.30;TLOD=41.89 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:5,15:0.777:20:1,5:4,7:3,2,11,4 -K03455 2169 . A G . . AS_SB_TABLE=0,0|17,6;DP=23;ECNT=41;MBQ=0,34;MFRL=0,232;MMQ=60,60;MPOS=32;POPAF=7.30;TLOD=105.32 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:0,23:0.955:23:0,9:0,13:0,0,17,6 -K03455 2183 . C T . . AS_SB_TABLE=0,0|19,9;DP=28;ECNT=41;MBQ=0,33;MFRL=0,233;MMQ=60,60;MPOS=28;POPAF=7.30;TLOD=133.75 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:0,28:0.962:28:0,9:0,14:0,0,19,9 -K03455 2189 . TA AA,AG . . AS_SB_TABLE=0,0|9,5|10,4;DP=30;ECNT=41;MBQ=0,35,20;MFRL=0,207,265;MMQ=60,60,60;MPOS=21,22;POPAF=7.30,7.30;TLOD=51.22,55.00 GT:AD:AF:DP:F1R2:F2R1:SB 0/1/2:0,14,14:0.424,0.539:28:0,6,5:0,7,5:0,0,19,9 -K03455 2195 . C G,T . . AS_SB_TABLE=5,4|10,4|5,2;DP=30;ECNT=41;MBQ=37,30,33;MFRL=232,265,241;MMQ=60,60,60;MPOS=19,19;POPAF=7.30,7.30;TLOD=41.44,19.27 GT:AD:AF:DP:F1R2:F2R1:SB 0/1/2:9,14,7:0.500,0.250:30:3,8,3:5,6,4:5,4,15,6 -K03455 2197 . A G . . AS_SB_TABLE=15,6|5,4;DP=30;ECNT=41;MBQ=31,37;MFRL=248,232;MMQ=60,60;MPOS=10;POPAF=7.30;TLOD=29.34 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:21,9:0.259:30:9,3:8,5:15,6,5,4 -K03455 2200 . ACTC A . . AS_SB_TABLE=16,8|3,2;DP=31;ECNT=41;MBQ=24,36;MFRL=265,232;MMQ=60,60;MPOS=17;POPAF=7.30;TLOD=13.22 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:24,5:0.153:29:10,3:7,2:0|1:2200_ACTC_A:2200:16,8,3,2 -K03455 2202 . T C . . AS_SB_TABLE=15,7|2,2;DP=31;ECNT=41;MBQ=34,35;MFRL=257,182;MMQ=60,60;MPOS=12;POPAF=7.30;TLOD=5.58 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:22,4:0.166:26:9,1:7,1:0|1:2202_T_C:2202:15,7,2,2 -K03455 2205 . C A . . AS_SB_TABLE=20,9|1,1;DP=31;ECNT=41;MBQ=34,26;MFRL=248,286;MMQ=60,60;MPOS=15;POPAF=7.30;TLOD=5.06 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:29,2:0.107:31:13,1:11,0:0|1:2202_T_C:2202:20,9,1,1 -K03455 2213 . A G . . AS_SB_TABLE=4,3|17,7;DP=31;ECNT=41;MBQ=38,37;MFRL=232,265;MMQ=60,60;MPOS=23;POPAF=7.30;TLOD=107.32 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:7,24:0.793:31:4,11:2,8:4,3,17,7 -K03455 2214 . G A . . AS_SB_TABLE=17,9|1,1;DP=28;ECNT=41;MBQ=33,26;MFRL=254,286;MMQ=60,60;MPOS=24;POPAF=7.30;TLOD=5.06 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:26,2:0.107:28:12,1:10,0:0|1:2214_G_A:2214:17,9,1,1 -K03455 2221 . C A . . AS_SB_TABLE=22,8|3,2;DP=35;ECNT=41;MBQ=34,35;MFRL=251,232;MMQ=60,60;MPOS=36;POPAF=7.30;TLOD=12.67 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:30,5:0.114:35:16,1:11,2:0|1:2200_ACTC_A:2200:22,8,3,2 -K03455 2223 . G A . . AS_SB_TABLE=16,3|7,7;DP=34;ECNT=41;MBQ=37,34;MFRL=253,211;MMQ=60,60;MPOS=33;POPAF=7.30;TLOD=47.83 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:19,14:0.461:33:12,7:7,6:16,3,7,7 -K03455 2224 . AT GG,CT . . AS_SB_TABLE=11,8|2,2|10,0;DP=33;ECNT=41;MBQ=34,29,38;MFRL=265,207,253;MMQ=60,60,60;MPOS=36,18;POPAF=7.30,7.30;TLOD=9.64,33.76 GT:AD:AF:DP:F1R2:F2R1:SB 0/1/2:19,4,10:0.091,0.333:33:9,2,7:8,2,3:11,8,12,2 -K03455 2225 . T G . . AS_SB_TABLE=20,7|1,1;DP=33;ECNT=41;MBQ=37,38;MFRL=253,286;MMQ=60,60;MPOS=35;POPAF=7.30;TLOD=5.14 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:27,2:0.099:29:16,1:11,1:0|1:2214_G_A:2214:20,7,1,1 -K03455 2226 . A G . . AS_SB_TABLE=3,3|20,7;DP=33;ECNT=41;MBQ=20,37;MFRL=232,253;MMQ=60,60;MPOS=20;POPAF=7.30;TLOD=115.36 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:6,27:0.844:33:3,16:2,9:0|1:2226_A_G:2226:3,3,20,7 -K03455 2230 . A C . . AS_SB_TABLE=3,3|21,7;DP=34;ECNT=41;MBQ=28,37;MFRL=232,253;MMQ=60,60;MPOS=24;POPAF=7.30;TLOD=119.79 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:6,28:0.848:34:2,15:3,11:0|1:2226_A_G:2226:3,3,21,7 -K03455 2234 . A G . . AS_SB_TABLE=3,3|24,10;DP=40;ECNT=41;MBQ=20,37;MFRL=232,245;MMQ=60,60;MPOS=15;POPAF=7.30;TLOD=146.51 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:6,34:0.865:40:1,20:2,11:0|1:2226_A_G:2226:3,3,24,10 -K03455 2235 . AC GA . . AS_SB_TABLE=25,11|2,2;DP=40;ECNT=41;MBQ=35,33;MFRL=247,207;MMQ=60,60;MPOS=45;POPAF=7.30;TLOD=9.17 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:36,4:0.081:40:22,1:10,2:0|1:2200_ACTC_A:2200:25,11,2,2 -K03455 2236 . CTG C,GTG . . AS_SB_TABLE=12,2|12,8|1,1;DP=40;ECNT=41;MBQ=38,35,38;MFRL=251,240,286;MMQ=60,60,60;MPOS=16,46;POPAF=7.30,7.30;TLOD=62.15,5.20 GT:AD:AF:DP:F1R2:F2R1:SB 0/1/2:14,20,2:0.528,0.083:36:8,12,1:5,8,1:12,2,13,9 -K03455 2239 . TA CC . . AS_SB_TABLE=16,11|12,2;DP=43;ECNT=41;MBQ=36,38;MFRL=232,251;MMQ=60,60;MPOS=30;POPAF=7.30;TLOD=49.19 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:27,14:0.368:41:14,9:10,4:16,11,12,2 -K03455 2240 . A ACC . . AS_SB_TABLE=4,3|14,10;DP=43;ECNT=41;MBQ=37,34;MFRL=232,211;MMQ=60,60;MPOS=19;POPAF=7.30;TLOD=61.81 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:7,24:0.785:31:3,13:3,9:4,3,14,10 -K03455 2248 . A G . . AS_SB_TABLE=3,2|27,12;DP=44;ECNT=41;MBQ=20,38;MFRL=182,245;MMQ=60,60;MPOS=28;POPAF=7.30;TLOD=182.06 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 1|0:5,39:0.902:44:2,21:3,15:1|0:2200_ACTC_A:2200:3,2,27,12 -K03455 2250 . T C . . AS_SB_TABLE=27,12|3,2;DP=44;ECNT=41;MBQ=38,37;MFRL=245,182;MMQ=60,60;MPOS=60;POPAF=7.30;TLOD=12.44 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:39,5:0.098:44:23,2:16,3:0|1:2200_ACTC_A:2200:27,12,3,2 -K03455 2258 . GG AA . . AS_SB_TABLE=6,0|26,14;DP=46;ECNT=41;MBQ=39,39;MFRL=216,239;MMQ=60,60;MPOS=38;POPAF=7.30;TLOD=159.02 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:6,40:0.859:46:3,22:3,17:0|1:2258_GG_AA:2258:6,0,26,14 -K03455 2259 . G A . . AS_SB_TABLE=0,0|10,3;DP=46;ECNT=41;MBQ=0,37;MFRL=0,241;MMQ=60,60;MPOS=39;POPAF=7.30;TLOD=13.48 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 1|0:0,13:0.961:13:0,5:0,8:1|0:2258_GG_AA:2258:0,0,10,3 -K03455 2282 . C T . . AS_SB_TABLE=32,24|2,2;DP=60;ECNT=41;MBQ=36,20;MFRL=239,182;MMQ=60,60;MPOS=44;POPAF=7.30;TLOD=9.01 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:56,4:0.059:60:24,1:24,2:32,24,2,2 -K03455 2285 . C T . . AS_SB_TABLE=30,23|6,3;DP=62;ECNT=41;MBQ=29,38;MFRL=236,205;MMQ=60,60;MPOS=49;POPAF=7.30;TLOD=26.48 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:53,9:0.151:62:24,3:21,4:30,23,6,3 -K03455 2287 . CA AT . . AS_SB_TABLE=32,26|4,3;DP=65;ECNT=41;MBQ=33,37;MFRL=234,285;MMQ=60,60;MPOS=41;POPAF=7.30;TLOD=17.41 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:58,7:0.133:65:25,1:24,2:32,26,4,3 -K03455 2300 . G A . . AS_SB_TABLE=6,6|26,23;DP=63;ECNT=41;MBQ=31,32;MFRL=229,236;MMQ=60,60;MPOS=37;POPAF=7.30;TLOD=211.84 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:12,49:0.796:61:3,20:5,22:6,6,26,23 -K03455 2303 . GCA G . . AS_SB_TABLE=7,8|25,20;DP=61;ECNT=41;MBQ=36,31;MFRL=225,241;MMQ=60,60;MPOS=40;POPAF=7.30;TLOD=171.69 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:15,45:0.735:60:2,18:9,20:7,8,25,20 -K03455 2304 . CAA GTG . . AS_SB_TABLE=6,6|10,9;DP=61;ECNT=41;MBQ=33,20;MFRL=229,235;MMQ=60,60;MPOS=38;POPAF=7.30;TLOD=3.47 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:12,19:0.531:31:4,0:7,3:6,6,10,9 -K03455 2306 . A ATG,G . . AS_SB_TABLE=2,2|26,22|4,4;DP=60;ECNT=41;MBQ=29,32,33;MFRL=182,239,280;MMQ=60,60,60;MPOS=39,19;POPAF=7.30,7.30;TLOD=175.97,22.08 GT:AD:AF:DP:F1R2:F2R1:SB 0/1/2:4,48,8:0.779,0.164:60:2,20,2:2,23,4:2,2,30,26 -K03455 2315 . A G . . AS_SB_TABLE=19,19|11,9;DP=59;ECNT=41;MBQ=37,38;MFRL=229,244;MMQ=60,60;MPOS=51;POPAF=7.30;TLOD=74.64 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:38,20:0.379:58:17,6:15,14:0|1:2315_A_G:2315:19,19,11,9 -K03455 2321 . A C . . AS_SB_TABLE=17,19|11,9;DP=56;ECNT=41;MBQ=34,37;MFRL=229,244;MMQ=60,60;MPOS=49;POPAF=7.30;TLOD=75.24 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:36,20:0.392:56:16,6:17,14:0|1:2315_A_G:2315:17,19,11,9 -K03455 2349 . T C . . AS_SB_TABLE=23,24|4,5;DP=56;ECNT=9;MBQ=35,35;MFRL=235,285;MMQ=60,60;MPOS=54;POPAF=7.30;TLOD=28.30 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:47,9:0.190:56:19,3:22,5:23,24,4,5 -K03455 2360 . G A . . AS_SB_TABLE=19,20|4,6;DP=49;ECNT=9;MBQ=37,27;MFRL=235,272;MMQ=60,60;MPOS=34;POPAF=7.30;TLOD=28.07 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:39,10:0.229:49:15,3:22,3:19,20,4,6 -K03455 2362 . G A . . AS_SB_TABLE=0,0|21,26;DP=49;ECNT=9;MBQ=0,36;MFRL=0,241;MMQ=60,60;MPOS=29;POPAF=7.30;TLOD=181.58 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:0,47:0.978:47:0,19:0,25:0,0,21,26 -K03455 2372 . A G . . AS_SB_TABLE=16,19|4,5;DP=44;ECNT=9;MBQ=37,36;MFRL=241,259;MMQ=60,60;MPOS=31;POPAF=7.30;TLOD=30.36 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:35,9:0.234:44:9,3:17,5:16,19,4,5 -K03455 2374 . G A . . AS_SB_TABLE=12,22|2,2;DP=38;ECNT=9;MBQ=37,35;MFRL=234,284;MMQ=60,60;MPOS=7;POPAF=7.30;TLOD=7.75 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:34,4:0.118:38:8,1:20,2:12,22,2,2 -K03455 2423 . A G . . AS_SB_TABLE=0,13|0,3;DP=16;ECNT=9;MBQ=34,33;MFRL=255,199;MMQ=60,60;MPOS=16;POPAF=7.30;TLOD=8.83 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:13,3:0.226:16:3,0:9,3:0,13,0,3 -K03455 2436 . A G . . AS_SB_TABLE=0,1|0,4;DP=6;ECNT=9;MBQ=16,31;MFRL=255,240;MMQ=60,60;MPOS=4;POPAF=7.30;TLOD=16.53 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:1,4:0.725:5:0,0:0,4:0,1,0,4 -K03455 2438 . A G . . AS_SB_TABLE=0,3|0,2;DP=6;ECNT=9;MBQ=29,33;MFRL=255,255;MMQ=60,60;MPOS=3;POPAF=7.30;TLOD=7.20 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:3,2:0.430:5:0,0:2,2:0|1:2438_A_G:2438:0,3,0,2 -K03455 2440 . T C . . AS_SB_TABLE=0,3|0,2;DP=6;ECNT=9;MBQ=0,31;MFRL=255,255;MMQ=60,60;MPOS=1;POPAF=7.30;TLOD=7.20 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:3,2:0.430:5:0,0:0,2:0|1:2438_A_G:2438:0,3,0,2 +K03455 2097 . G A . . AS_SB_TABLE=0,0|10,4;DP=14;ECNT=48;ECNTH=22;MBQ=0,20;MFRL=0,160;MMQ=60,60;MPOS=34;POPAF=7.30;TLOD=75.42 GT:AD:AF:DP:F1R2:F2R1:FAD:SB 0/1:0,14:0.923:14:0,6:0,3:0,11:0,0,10,4 +K03455 2106 . T C . . AS_SB_TABLE=9,4|2,0;DP=15;ECNT=48;ECNTH=22;MBQ=20,39;MFRL=159,186;MMQ=60,60;MPOS=39;POPAF=7.30;TLOD=10.57 GT:AD:AF:DP:F1R2:F2R1:FAD:SB 0/1:13,2:0.214:15:5,1:5,0:10,2:9,4,2,0 +K03455 2110 . TA CA,CT . . AS_SB_TABLE=0,0|9,4|3,1;DP=17;ECNT=48;ECNTH=22,18;MBQ=0,20,32;MFRL=0,159,161;MMQ=60,60,60;MPOS=46,2;POPAF=7.30,7.30;TLOD=55.66,10.78 GT:AD:AF:DP:F1R2:F2R1:FAD:SB 0/1/2:0,13,4:0.738,0.200:17:0,5,1:0,5,1:0,10,2:0,0,12,5 +K03455 2118 . A G . . AS_SB_TABLE=3,1|9,4;DP=17;ECNT=48;ECNTH=16;MBQ=26,20;MFRL=161,159;MMQ=60,60;MPOS=55;POPAF=7.30;TLOD=51.30 GT:AD:AF:DP:F1R2:F2R1:FAD:SB 0/1:4,13:0.787:17:1,4:1,4:2,10:3,1,9,4 +K03455 2130 . T C . . AS_SB_TABLE=9,4|3,1;DP=17;ECNT=48;ECNTH=18;MBQ=20,29;MFRL=159,161;MMQ=60,60;MPOS=22;POPAF=7.30;TLOD=10.55 GT:AD:AF:DP:F1R2:F2R1:FAD:SB 0/1:13,4:0.213:17:4,1:6,1:10,2:9,4,3,1 +K03455 2136 . T C . . AS_SB_TABLE=9,4|3,1;DP=17;ECNT=48;ECNTH=18;MBQ=20,30;MFRL=159,161;MMQ=60,60;MPOS=28;POPAF=7.30;TLOD=10.55 GT:AD:AF:DP:F1R2:F2R1:FAD:SB 0/1:13,4:0.213:17:4,1:6,1:10,2:9,4,3,1 +K03455 2155 . A T . . AS_SB_TABLE=3,1|11,4;DP=19;ECNT=48;ECNTH=22;MBQ=30,20;MFRL=161,233;MMQ=60,60;MPOS=56;POPAF=7.30;TLOD=56.42 GT:AD:AF:DP:F1R2:F2R1:FAD:SB 0/1:4,15:0.823:19:1,5:1,5:2,13:3,1,11,4 +K03455 2169 . A G . . AS_SB_TABLE=0,0|17,5;DP=22;ECNT=48;ECNTH=22;MBQ=0,33;MFRL=0,233;MMQ=60,60;MPOS=34;POPAF=7.30;TLOD=101.65 GT:AD:AF:DP:F1R2:F2R1:FAD:SB 0/1:0,22:0.952:22:0,9:0,9:0,19:0,0,17,5 +K03455 2183 . C T . . AS_SB_TABLE=0,0|19,9;DP=28;ECNT=48;ECNTH=22;MBQ=0,20;MFRL=0,233;MMQ=60,60;MPOS=28;POPAF=7.30;TLOD=142.60 GT:AD:AF:DP:F1R2:F2R1:FAD:SB 0/1:0,28:0.962:28:0,10:0,11:0,24:0,0,19,9 +K03455 2189 . TA AA,AG . . AS_SB_TABLE=0,0|9,5|10,5;DP=31;ECNT=48;ECNTH=17,22;MBQ=0,35,20;MFRL=0,207,265;MMQ=60,60,60;MPOS=21,22;POPAF=7.30,7.30;TLOD=46.96,59.29 GT:AD:AF:DP:F1R2:F2R1:FAD:SB 0/1/2:0,14,15:0.408,0.556:29:0,5,6:0,4,5:0,10,14:0,0,19,10 +K03455 2195 . C G,T . . AS_SB_TABLE=5,5|11,4|4,2;DP=31;ECNT=48;ECNTH=22,18;MBQ=33,30,38;MFRL=207,265,204;MMQ=60,60,60;MPOS=18,23;POPAF=7.30,7.30;TLOD=55.86,17.17 GT:AD:AF:DP:F1R2:F2R1:FAD:SB 0/1/2:10,15,6:0.514,0.210:31:3,8,2:3,6,3:7,14,5:5,5,15,6 +K03455 2197 . A G . . AS_SB_TABLE=15,7|5,4;DP=31;ECNT=48;ECNTH=17;MBQ=31,37;MFRL=245,232;MMQ=60,60;MPOS=10;POPAF=7.30;TLOD=29.21 GT:AD:AF:DP:F1R2:F2R1:FAD:SB 0/1:22,9:0.250:31:10,3:7,3:20,6:15,7,5,4 +K03455 2200 . ACTC A . . AS_SB_TABLE=16,9|3,3;DP=31;ECNT=48;ECNTH=17;MBQ=22,35;MFRL=265,207;MMQ=60,60;MPOS=15;POPAF=7.30;TLOD=17.33 GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB 0|1:25,6:0.179:31:10,2:6,1:22,4:0|1:2200_ACTC_A:2200:16,9,3,3 +K03455 2202 . T C . . AS_SB_TABLE=15,9|1,1;DP=31;ECNT=48;ECNTH=15;MBQ=27,35;MFRL=245,286;MMQ=60,60;MPOS=12;POPAF=7.30;TLOD=5.33 GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB 0|1:24,2:0.118:26:9,1:6,1:21,2:0|1:2202_T_C:2202:15,9,1,1 +K03455 2205 . C A . . AS_SB_TABLE=20,11|1,1;DP=33;ECNT=48;ECNTH=15;MBQ=20,26;MFRL=241,286;MMQ=60,60;MPOS=15;POPAF=7.30;TLOD=4.96 GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB 0|1:31,2:0.100:33:12,1:8,0:26,2:0|1:2202_T_C:2202:20,11,1,1 +K03455 2213 . A G . . AS_SB_TABLE=4,4|17,7;DP=32;ECNT=48;ECNTH=22;MBQ=20,37;MFRL=232,265;MMQ=60,60;MPOS=23;POPAF=7.30;TLOD=107.92 GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB 0|1:8,24:0.767:32:4,10:1,7:6,22:0|1:2213_A_G:2213:4,4,17,7 +K03455 2214 . G A . . AS_SB_TABLE=17,10|1,1;DP=29;ECNT=48;ECNTH=19;MBQ=32,26;MFRL=248,286;MMQ=60,60;MPOS=24;POPAF=7.30;TLOD=5.01 GT:AD:AF:DP:F1R2:F2R1:FAD:SB 0/1:27,2:0.103:29:11,1:8,0:25,2:17,10,1,1 +K03455 2221 . C A . . AS_SB_TABLE=22,8|3,2;DP=35;ECNT=48;ECNTH=17;MBQ=34,20;MFRL=251,232;MMQ=60,60;MPOS=36;POPAF=7.30;TLOD=12.95 GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB 0|1:30,5:0.111:35:16,1:9,1:31,3:0|1:2200_ACTC_A:2200:22,8,3,2 +K03455 2223 . G A . . AS_SB_TABLE=17,4|6,6;DP=34;ECNT=48;ECNTH=22;MBQ=34,33;MFRL=248,250;MMQ=60,60;MPOS=45;POPAF=7.30;TLOD=43.41 GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB 0|1:21,12:0.400:33:13,5:6,4:19,12:0|1:2223_G_A:2223:17,4,6,6 +K03455 2224 . AT GG,CT . . AS_SB_TABLE=11,8|2,2|10,0;DP=33;ECNT=48;ECNTH=17,19;MBQ=34,20,38;MFRL=265,207,253;MMQ=60,60,60;MPOS=36,18;POPAF=7.30,7.30;TLOD=9.60,31.07 GT:AD:AF:DP:F1R2:F2R1:FAD:SB 0/1/2:19,4,10:0.088,0.324:33:10,1,7:5,1,3:19,2,10:11,8,12,2 +K03455 2225 . T G . . AS_SB_TABLE=20,7|1,1;DP=33;ECNT=48;ECNTH=15;MBQ=37,38;MFRL=253,286;MMQ=60,60;MPOS=35;POPAF=7.30;TLOD=5.35 GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB 0|1:27,2:0.096:29:16,1:8,1:27,2:0|1:2202_T_C:2202:20,7,1,1 +K03455 2226 . A G . . AS_SB_TABLE=3,4|20,6;DP=33;ECNT=48;ECNTH=22;MBQ=20,37;MFRL=232,253;MMQ=60,60;MPOS=21;POPAF=7.30;TLOD=123.69 GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB 0|1:7,26:0.818:33:3,15:1,7:5,26:0|1:2213_A_G:2213:3,4,20,6 +K03455 2230 . A C . . AS_SB_TABLE=3,4|21,6;DP=34;ECNT=48;ECNTH=22;MBQ=20,37;MFRL=232,253;MMQ=60,60;MPOS=25;POPAF=7.30;TLOD=128.16 GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB 0|1:7,27:0.824:34:3,15:2,8:5,27:0|1:2213_A_G:2213:3,4,21,6 +K03455 2234 . A G . . AS_SB_TABLE=3,4|24,7;DP=38;ECNT=48;ECNTH=22;MBQ=20,37;MFRL=232,248;MMQ=60,60;MPOS=22;POPAF=7.30;TLOD=145.81 GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB 0|1:7,31:0.842:38:2,18:1,9:5,31:0|1:2213_A_G:2213:3,4,24,7 +K03455 2235 . AC GA . . AS_SB_TABLE=25,9|2,2;DP=38;ECNT=48;ECNTH=17;MBQ=34,20;MFRL=251,207;MMQ=60,60;MPOS=45;POPAF=7.30;TLOD=9.13 GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB 0|1:34,4:0.079:38:20,1:8,1:34,2:0|1:2200_ACTC_A:2200:25,9,2,2 +K03455 2236 . CTG C,GTG . . AS_SB_TABLE=14,1|10,6|1,1;DP=38;ECNT=48;ECNTH=22,15;MBQ=38,34,38;MFRL=253,240,286;MMQ=60,60,60;MPOS=25,46;POPAF=7.30,7.30;TLOD=57.94,5.24 GT:AD:AF:DP:F1R2:F2R1:FAD:SB 0/1/2:15,16,2:0.481,0.083:33:9,8,1:5,5,1:15,16,2:14,1,11,7 +K03455 2239 . TA CC . . AS_SB_TABLE=14,10|14,1;DP=44;ECNT=48;ECNTH=18;MBQ=32,38;MFRL=232,253;MMQ=60,60;MPOS=27;POPAF=7.30;TLOD=48.64 GT:AD:AF:DP:F1R2:F2R1:FAD:SB 0/1:24,15:0.400:39:11,10:6,4:22,15:14,10,14,1 +K03455 2240 . A ACC . . AS_SB_TABLE=4,5|14,7;DP=44;ECNT=48;ECNTH=22;MBQ=20,34;MFRL=182,235;MMQ=60,60;MPOS=19;POPAF=7.30;TLOD=57.61 GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB 0|1:9,21:0.736:30:3,10:3,6:7,20:0|1:2223_G_A:2223:4,5,14,7 +K03455 2248 . A G . . AS_SB_TABLE=3,2|27,13;DP=45;ECNT=48;ECNTH=22;MBQ=20,37;MFRL=182,245;MMQ=60,60;MPOS=29;POPAF=7.30;TLOD=186.70 GT:AD:AF:DP:F1R2:F2R1:FAD:SB 0/1:5,40:0.905:45:1,19:2,12:3,37:3,2,27,13 +K03455 2250 . T C . . AS_SB_TABLE=27,13|3,2;DP=45;ECNT=48;ECNTH=17;MBQ=37,20;MFRL=245,182;MMQ=60,60;MPOS=60;POPAF=7.30;TLOD=12.39 GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB 0|1:40,5:0.095:45:21,1:13,2:37,3:0|1:2200_ACTC_A:2200:27,13,3,2 +K03455 2258 . GG AA . . AS_SB_TABLE=6,1|25,14;DP=46;ECNT=48;ECNTH=22;MBQ=34,38;MFRL=191,243;MMQ=60,60;MPOS=38;POPAF=7.30;TLOD=153.60 GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB 0|1:7,39:0.851:46:3,19:3,12:6,35:0|1:2258_GG_AA:2258:6,1,25,14 +K03455 2259 . G A . . AS_SB_TABLE=0,0|8,5;DP=46;ECNT=48;ECNTH=19;MBQ=0,33;MFRL=0,187;MMQ=60,60;MPOS=26;POPAF=7.30;TLOD=14.22 GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB 1|0:0,13:0.963:13:0,8:0,8:0,19:1|0:2258_GG_AA:2258:0,0,8,5 +K03455 2273 . A G . . AS_SB_TABLE=33,13|0,2;DP=48;ECNT=48;ECNTH=19;MBQ=36,27;MFRL=234,225;MMQ=60,60;MPOS=61;POPAF=7.30;TLOD=3.75 GT:AD:AF:DP:F1R2:F2R1:FAD:SB 0/1:46,2:0.065:48:22,1:15,1:42,2:33,13,0,2 +K03455 2282 . C T . . AS_SB_TABLE=31,24|2,2;DP=60;ECNT=48;ECNTH=16;MBQ=20,20;MFRL=241,182;MMQ=60,60;MPOS=44;POPAF=7.30;TLOD=9.01 GT:AD:AF:DP:F1R2:F2R1:FAD:SB 0/1:55,4:0.060:59:19,0:18,1:46,2:31,24,2,2 +K03455 2285 . C T . . AS_SB_TABLE=30,24|3,3;DP=60;ECNT=48;ECNTH=16;MBQ=20,28;MFRL=236,207;MMQ=60,60;MPOS=52;POPAF=7.30;TLOD=15.47 GT:AD:AF:DP:F1R2:F2R1:FAD:SB 0/1:54,6:0.098:60:20,2:17,2:45,4:30,24,3,3 +K03455 2287 . CA AT . . AS_SB_TABLE=32,27|1,1;DP=61;ECNT=48;ECNTH=18;MBQ=20,38;MFRL=232,286;MMQ=60,60;MPOS=52;POPAF=7.30;TLOD=4.18 GT:AD:AF:DP:F1R2:F2R1:FAD:SB 0/1:59,2:0.057:61:22,1:17,1:49,2:32,27,1,1 +K03455 2300 . G A . . AS_SB_TABLE=3,4|26,24;DP=57;ECNT=48;ECNTH=22;MBQ=36,34;MFRL=182,236;MMQ=60,60;MPOS=37;POPAF=7.30;TLOD=226.05 GT:AD:AF:DP:F1R2:F2R1:FAD:SB 0/1:7,50:0.880:57:3,15:1,16:5,43:3,4,26,24 +K03455 2303 . GCA G . . AS_SB_TABLE=4,6|25,20;DP=56;ECNT=48;ECNTH=22;MBQ=28,31;MFRL=204,241;MMQ=60,60;MPOS=40;POPAF=7.30;TLOD=181.84 GT:AD:AF:DP:F1R2:F2R1:FAD:SB 0/1:10,45:0.812:55:1,15:4,15:8,38:4,6,25,20 +K03455 2304 . CAA GTG . . AS_SB_TABLE=3,4|9,9;DP=56;ECNT=48;ECNTH=18;MBQ=35,31;MFRL=182,232;MMQ=60,60;MPOS=36;POPAF=7.30;TLOD=4.64 GT:AD:AF:DP:F1R2:F2R1:FAD:SB 0/1:7,18:0.643:25:3,0:2,3:6,16:3,4,9,9 +K03455 2306 . A ATG,G . . AS_SB_TABLE=2,3|26,22|1,1;DP=55;ECNT=48;ECNTH=22,15;MBQ=20,34,36;MFRL=182,239,286;MMQ=60,60,60;MPOS=39,33;POPAF=7.30,7.30;TLOD=184.31,4.73 GT:AD:AF:DP:F1R2:F2R1:FAD:SB 0/1/2:5,48,2:0.857,0.061:55:2,16,1:1,17,1:3,41,2:2,3,27,23 +K03455 2315 . A G . . AS_SB_TABLE=16,17|11,9;DP=54;ECNT=48;ECNTH=22;MBQ=33,38;MFRL=202,244;MMQ=60,60;MPOS=51;POPAF=7.30;TLOD=75.73 GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB 0|1:33,20:0.418:53:12,5:9,10:27,19:0|1:2315_A_G:2315:16,17,11,9 +K03455 2321 . A C . . AS_SB_TABLE=14,17|11,9;DP=51;ECNT=48;ECNTH=22;MBQ=33,37;MFRL=202,244;MMQ=60,60;MPOS=49;POPAF=7.30;TLOD=76.39 GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB 0|1:31,20:0.435:51:10,5:9,10:25,19:0|1:2315_A_G:2315:14,17,11,9 +K03455 2349 . T C . . AS_SB_TABLE=16,23|4,5;DP=49;ECNT=9;ECNTH=6;MBQ=35,35;MFRL=235,259;MMQ=60,60;MPOS=54;POPAF=7.30;TLOD=26.85 GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB 0|1:39,9:0.211:48:13,3:18,5:36,9:0|1:2349_T_C:2349:16,23,4,5 +K03455 2360 . G A . . AS_SB_TABLE=15,20|3,5;DP=43;ECNT=9;ECNTH=6;MBQ=35,36;MFRL=235,246;MMQ=60,60;MPOS=56;POPAF=7.30;TLOD=23.78 GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB 0|1:35,8:0.213:43:11,3:17,3:32,8:0|1:2349_T_C:2349:15,20,3,5 +K03455 2362 . G A . . AS_SB_TABLE=0,0|16,25;DP=43;ECNT=9;ECNTH=3;MBQ=0,36;MFRL=0,235;MMQ=60,60;MPOS=34;POPAF=7.30;TLOD=152.64 GT:AD:AF:DP:F1R2:F2R1:FAD:SB 0/1:0,41:0.976:41:0,13:0,21:0,38:0,0,16,25 +K03455 2372 . A G . . AS_SB_TABLE=12,18|4,5;DP=39;ECNT=9;ECNTH=6;MBQ=37,36;MFRL=227,232;MMQ=60,60;MPOS=31;POPAF=7.30;TLOD=28.25 GT:AD:AF:DP:F1R2:F2R1:FAD:SB 0/1:30,9:0.263:39:3,3:16,5:27,9:12,18,4,5 +K03455 2422 . G A . . AS_SB_TABLE=0,14|0,2;DP=16;ECNT=9;ECNTH=3;MBQ=34,36;MFRL=239,219;MMQ=60,60;MPOS=9;POPAF=7.30;TLOD=5.69 GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB 0|1:14,2:0.167:16:0,0:9,2:14,2:0|1:2422_G_A:2422:0,14,0,2 +K03455 2426 . G A . . AS_SB_TABLE=0,14|0,2;DP=16;ECNT=9;ECNTH=3;MBQ=37,35;MFRL=239,219;MMQ=60,60;MPOS=5;POPAF=7.30;TLOD=5.69 GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB 0|1:14,2:0.167:16:0,0:9,2:14,2:0|1:2422_G_A:2422:0,14,0,2 +K03455 2436 . A G . . AS_SB_TABLE=0,0|0,0;DP=3;ECNT=9;ECNTH=4;MBQ=0,31;MFRL=0,255;MMQ=60,60;MPOS=5;POPAF=7.30;TLOD=8.49 GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB 0|1:0,2:0.766:2:0,0:0,2:0,2:0|1:2436_A_G:2436:0,0,0,2 +K03455 2438 . A G . . AS_SB_TABLE=0,0|0,0;DP=3;ECNT=9;ECNTH=4;MBQ=0,33;MFRL=0,255;MMQ=60,60;MPOS=3;POPAF=7.30;TLOD=8.49 GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB 0|1:0,2:0.766:2:0,0:0,2:0,2:0|1:2436_A_G:2436:0,0,0,2 +K03455 2440 . T C . . AS_SB_TABLE=0,0|0,0;DP=3;ECNT=9;ECNTH=4;MBQ=0,31;MFRL=0,255;MMQ=60,60;MPOS=1;POPAF=7.30;TLOD=8.49 GT:AD:AF:DP:F1R2:F2R1:FAD:PGT:PID:PS:SB 0|1:0,2:0.766:2:0,0:0,2:0,2:0|1:2436_A_G:2436:0,0,0,2