Mercurial > repos > iuc > featurecounts
annotate featurecounts.xml @ 37:8620533d294b draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit c43e4b51d4b0a46eeba363fa4061d4af03a677a4
| author | iuc |
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| date | Wed, 07 May 2025 09:59:13 +0000 |
| parents | 39863c075dca |
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| rev | line source |
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37
8620533d294b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit c43e4b51d4b0a46eeba363fa4061d4af03a677a4
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1 <tool id="featurecounts" name="featureCounts" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="23.2"> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit e0d4688a59e6eeba33adcfe803ac43d0bc2863e7"
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2 <description>Measure gene expression in RNA-Seq experiments from SAM or BAM files</description> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit c2db6daac834eea9b88388aed840d484544f0786"
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3 <macros> |
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8620533d294b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit c43e4b51d4b0a46eeba363fa4061d4af03a677a4
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4 <token name="@TOOL_VERSION@">2.1.1</token> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit eb563889f227d5c473e2bad2cf9a513e41d03fca
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5 <token name="@VERSION_SUFFIX@">0</token> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
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6 <macro name="conditional_gff_opions"> |
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456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
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7 <param name="gff_feature_type" type="text" value="exon" argument="-t" label="GFF feature type filter" help="Specify the feature type. Only rows which have the matched matched feature type in the provided GTF annotation file will be included for read counting. `exon' by default."/> |
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456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
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8 <param name="gff_feature_attribute" type="text" value="gene_id" argument="-g" label="GFF gene identifier" help="Specify the attribute type used to group features (eg. exons) into meta-features (eg. genes), when GTF annotation is provided. `gene_id' by default. This attribute type is usually the gene identifier. This argument is useful for the meta-feature level summarization. Ex: if the 9th column is 'gene_id "ENSG00000223972"; gene_name "DDX11L1" gene_source "havana"' (GTF) or 'gene_id=ENSG00000223972; gene_name=DDX11L1; gene_source=havana' (GFF), the available attributes for this feature are 'gene_id', 'gene_name' and 'gene_source'."/> |
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456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
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9 <param name="summarization_level" type="boolean" truevalue=" -f" falsevalue="" argument="-f" label="On feature level" help="If specified, read summarization will be performed at the feature level. By default (-f is not specified), the read summarization is performed at the meta-feature level."/> |
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456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
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10 </macro> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit c2db6daac834eea9b88388aed840d484544f0786"
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11 </macros> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit e0d4688a59e6eeba33adcfe803ac43d0bc2863e7"
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12 <xrefs> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
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13 <xref type="bio.tools">featurecounts</xref> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit e0d4688a59e6eeba33adcfe803ac43d0bc2863e7"
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14 </xrefs> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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15 <requirements> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit c2db6daac834eea9b88388aed840d484544f0786"
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16 <requirement type="package" version="@TOOL_VERSION@">subread</requirement> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit eb563889f227d5c473e2bad2cf9a513e41d03fca
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17 <requirement type="package" version="1.21">samtools</requirement> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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18 </requirements> |
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8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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19 |
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8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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20 <version_command>featureCounts -v 2>&1 | grep .</version_command> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
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21 <command detect_errors="exit_code"><![CDATA[ |
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7d1e37c6ef85
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
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22 ## Export fc path for its built-in annotation |
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7d1e37c6ef85
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
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23 export FC_PATH=\$(command -v featureCounts | sed 's@/bin/featureCounts$@@') && |
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7d1e37c6ef85
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
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24 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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25 ## Check whether all alignments are from the same type (bam || sam) |
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8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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26 featureCounts |
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27 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 4c095ddb0f2c369d2bf3fc5ca386f6981a2fa0eb
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28 #if $anno.anno_select=="history": |
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d0beb2753aba
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 4c095ddb0f2c369d2bf3fc5ca386f6981a2fa0eb
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29 -a '$anno.reference_gene_sets' |
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d0beb2753aba
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 4c095ddb0f2c369d2bf3fc5ca386f6981a2fa0eb
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30 -F "GTF" |
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d0beb2753aba
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 4c095ddb0f2c369d2bf3fc5ca386f6981a2fa0eb
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31 #elif $anno.anno_select=="cached": |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
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32 -a '$anno.reference_gene_sets_cached.fields.path' |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
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33 -F "GTF" |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
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34 #elif $anno.anno_select=="builtin": |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 4c095ddb0f2c369d2bf3fc5ca386f6981a2fa0eb
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35 -a \${FC_PATH}/annotation/${anno.bgenome}_RefSeq_exon.txt |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
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36 -F "SAF" |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 1c0d28b6cefe154e8cf037c9f36200e8f52a838f
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37 #end if |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 1c0d28b6cefe154e8cf037c9f36200e8f52a838f
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38 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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39 -o "output" |
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40 -T \${GALAXY_SLOTS:-2} |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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41 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 650fa1433cf8fbc8689ed35a4d0340fc7a130aca
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42 -s $strand_specificity |
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43 |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 839f962c859728f53bb696cea0720862418f1a13"
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44 -Q $read_filtering_parameters.mapping_quality |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 839f962c859728f53bb696cea0720862418f1a13"
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45 $read_filtering_parameters.splitonly |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 839f962c859728f53bb696cea0720862418f1a13"
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46 $read_filtering_parameters.primary |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 839f962c859728f53bb696cea0720862418f1a13"
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47 $read_filtering_parameters.ignore_dup |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 839f962c859728f53bb696cea0720862418f1a13"
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48 |
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49 #if $anno.anno_select!="builtin": |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
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50 -t '$anno.gff_feature_type' |
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51 -g '$anno.gff_feature_attribute' |
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52 $anno.summarization_level |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
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53 #end if |
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54 |
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55 $extended_parameters.multifeatures.multifeat |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit d80704d28a7be0c42d41e48f8e12b9d727233be4"
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56 #if $extended_parameters.multifeatures.multifeat != "": |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit d80704d28a7be0c42d41e48f8e12b9d727233be4"
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57 $extended_parameters.multifeatures.fraction |
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58 #end if |
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59 |
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60 $extended_parameters.exon_exon_junction_read_counting_enabled.count_exon_exon_junction_reads |
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61 #if str($extended_parameters.exon_exon_junction_read_counting_enabled.count_exon_exon_junction_reads) == "-J": |
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62 #if $extended_parameters.exon_exon_junction_read_counting_enabled.genome: |
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63 -G '$extended_parameters.exon_exon_junction_read_counting_enabled.genome' |
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64 #end if |
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65 #end if |
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66 |
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67 $extended_parameters.long_reads |
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68 |
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69 $extended_parameters.by_read_group |
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70 |
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71 $extended_parameters.largest_overlap |
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72 --minOverlap $extended_parameters.min_overlap |
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73 --fracOverlap $extended_parameters.frac_overlap |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2228563ac1c2bb236c8ac83d08d2b86e21a39e41
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74 --fracOverlapFeature $extended_parameters.frac_overlap_feature |
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75 $extended_parameters.read_reduction |
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76 #if $extended_parameters.R: |
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77 $extended_parameters.R |
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78 #end if |
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79 #if str($extended_parameters.read_extension_5p) != "0": |
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80 --readExtension5 $extended_parameters.read_extension_5p |
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81 #end if |
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82 |
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83 #if str($extended_parameters.read_extension_3p) != "0": |
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84 --readExtension3 $extended_parameters.read_extension_3p |
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85 #end if |
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32
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86 |
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87 #if str($pe_parameters.paired_end_status) != "single_end": |
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88 -p |
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89 $pe_parameters.only_both_ends |
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90 $pe_parameters.exclude_chimerics |
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91 #if str($pe_parameters.paired_end_status) == "PE_fragments": |
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92 --countReadPairs |
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93 #if $pe_parameters.check_distance_enabled.checkFragLength == "true": |
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32
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94 --checkFragLength |
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95 --minFragLength $pe_parameters.check_distance_enabled.minimum_fragment_length |
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96 --maxFragLength $pe_parameters.check_distance_enabled.maximum_fragment_length |
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97 #end if |
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98 #end if |
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99 #end if |
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100 |
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6
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101 '${alignment}' |
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102 |
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103 ## Remove comment and add sample name to header |
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104 && grep -v "^#" "output" |
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105 | sed -e 's|${alignment}|${alignment.element_identifier}|g' |
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106 > body.txt |
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107 ## Set the right columns for the tabular formats |
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108 #if $format.value == "tabdel_medium": |
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109 && cut -f 1,7 body.txt > expression_matrix.txt |
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110 |
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111 ## Paste doesn't allow a non ordered list of columns: -f 1,7,8,6 will only return columns 1,7 and 8 |
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112 ## Thus the gene length column (last column) has to be added separately |
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113 && cut -f 6 body.txt > gene_lengths.txt |
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114 && paste expression_matrix.txt gene_lengths.txt > expression_matrix.txt.bak |
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115 && mv -f expression_matrix.txt.bak '${output_medium}' |
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116 #elif $format.value == "tabdel_short": |
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6
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117 && cut -f 1,7 body.txt > '${output_short}' |
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10
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118 #else: |
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6
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119 && cp body.txt '${output_full}' |
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120 #end if |
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8
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121 |
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122 #if str($include_feature_length_file) == "true": |
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6
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123 && cut -f 1,6 body.txt > '${output_feature_lengths}' |
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124 #end if |
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125 |
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126 #if str($extended_parameters.exon_exon_junction_read_counting_enabled.count_exon_exon_junction_reads) == "-J": |
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13
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127 && sed -e 's|${alignment}|${alignment.element_identifier}|g' 'output.jcounts' > '${output_jcounts}' |
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6
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128 #end if |
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129 |
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130 #if $extended_parameters.R: |
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28
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit c2db6daac834eea9b88388aed840d484544f0786"
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131 && samtools sort --no-PG -o '$output_bam' -@ \${GALAXY_SLOTS:-2} -T "\${TMPDIR:-.}" *.featureCounts.bam |
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132 #end if |
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13
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133 && sed -e 's|${alignment}|${alignment.element_identifier}|g' 'output.summary' > '${output_summary}' |
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134 ]]></command> |
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135 <inputs> |
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136 <param name="alignment" |
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137 type="data" |
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138 multiple="false" |
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34
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139 format="unsorted.bam,bam,sam" |
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140 label="Alignment file" |
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141 help="The input alignment file(s) where the gene expression has to be counted. The file can have a SAM or BAM format; but ALL files must be in the same format. Unless you are using a Gene annotation file from the History, these files must have the database/genome attribute already specified e.g. hg38, not the default: ?"> |
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142 </param> |
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143 |
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144 <param name="strand_specificity" |
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145 type="select" |
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146 label="Specify strand information" |
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147 argument="-s" |
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148 help="Indicate if the data is stranded and if strand-specific read counting should be performed. Strand setting must be the same as the strand settings used to produce the mapped BAM input(s)"> |
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149 <option value="0" selected="true">Unstranded</option> |
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150 <option value="1">Stranded (Forward)</option> |
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151 <option value="2">Stranded (Reverse)</option> |
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152 </param> |
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153 |
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154 <conditional name="anno"> |
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155 <param name="anno_select" type="select" label="Gene annotation file"> |
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156 <option value="builtin">featureCounts built-in</option> |
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157 <option value="cached" selected="True">locally cached</option> |
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158 <option value="history">A GFF/GTF file in your history</option> |
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159 </param> |
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160 <when value="builtin"> |
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161 <param name="bgenome" type="select" label="Select built-in genome" help="Built-in gene annotations for genomes hg38, hg19, mm10 and mm9 are included in featureCounts"> |
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162 <options from_data_table="featurecounts_anno"> |
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25
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163 <filter type="data_meta" key="dbkey" ref="alignment" column="dbkey"/> |
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164 <filter type="sort_by" column="1"/> |
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165 </options> |
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166 <validator type="no_options" message="An built-in annotation file is not available for the genome build associated with the selected input file"/> |
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167 </param> |
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168 </when> |
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169 <when value="cached"> |
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170 <param name="reference_gene_sets_cached" type="select" label="Using locally cached annotation" help="If the annotation file you require is not listed here, please contact the Galaxy administrator."> |
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171 <options from_data_table="gene_sets"> |
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25
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172 <filter type="data_meta" key="dbkey" ref="alignment" column="dbkey"/> |
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173 <filter type="sort_by" column="2"/> |
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174 </options> |
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175 <validator type="no_options" message="A cached annotation file is not available for the genome build associated with the selected input file"/> |
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11
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176 </param> |
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177 <expand macro="conditional_gff_opions"/> |
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178 </when> |
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179 <when value="history"> |
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180 <param name="reference_gene_sets" |
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181 format="gff,gtf,gff3" |
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182 type="data" |
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183 label="Gene annotation file" |
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184 help="The program assumes that the provided annotation file is in GFF/GTF format. Make sure that the gene annotation file corresponds to the same reference genome as used for the alignment."> |
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185 </param> |
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186 <expand macro="conditional_gff_opions"/> |
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187 </when> |
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188 </conditional> |
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189 |
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190 <param name="format" |
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191 type="select" |
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192 label="Output format" |
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193 help="The output format will be tabular, select the preferred columns here"> |
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194 <option value="tabdel_short">Gene-ID "\t" read-count (MultiQC/DESeq2/edgeR/limma-voom compatible)</option> |
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195 <option value="tabdel_medium">Gene-ID "\t" read-count "\t" gene-length</option> |
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196 <option value="tabdel_full">featureCounts 1.4.0+ default (includes regions provided by the GTF file)</option> |
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197 </param> |
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198 |
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199 <param name="include_feature_length_file" |
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200 type="boolean" |
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201 truevalue="true" |
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202 falsevalue="false" |
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203 checked="false" |
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204 label="Create gene-length file" |
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205 help="Creates a tabular file that contains the effective (nucleotides used for counting reads) length of the feature; might be useful for estimating FPKM/RPKM"/> |
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206 |
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207 <conditional name="pe_parameters"> |
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208 <param name="paired_end_status" type="select" label="Does the input have read pairs?" help="Were the bam files generated by aligning the output of a paired-end sequencing experiment? If yes, the tool can consider 2 reads = 1 read pair as 1 entity to count. Alternatively, you can opt to consider treating the read pairs as 2 individual reads to count seperately."> |
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209 <option value="single_end" selected="True">No, single-end.</option> |
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210 <option value="PE_individual">Yes, paired-end but still count them as if individual reads.</option> |
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211 <option value="PE_fragments">Yes, paired-end and count them as 1 single fragment.</option> |
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212 </param> |
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213 |
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214 <when value="single_end"/> |
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215 <when value="PE_individual"> |
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216 <param name="only_both_ends" |
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217 type="boolean" |
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218 truevalue=" -B" |
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219 falsevalue="" |
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220 argument="-B" |
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221 label="Only allow fragments with both reads aligned" |
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222 help="If specified, only fragments that have both ends successfully aligned will be considered for summarization. This option is only applicable for paired-end reads."/> |
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223 |
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224 <param name="exclude_chimerics" |
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225 type="boolean" |
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226 truevalue=" -C" |
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227 falsevalue="" |
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228 argument="-C" |
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229 checked="true" |
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230 label="Exclude chimeric fragments" |
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231 help="If specified, the chimeric fragments (those fragments that have their two ends aligned to different chromosomes) will NOT be included for summarization. This option is only applicable for paired-end read data."/> |
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232 </when> |
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233 <when value="PE_fragments"> |
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234 <conditional name="check_distance_enabled"> |
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235 <param argument="--checkFragLength" type="select" label="Check paired-end distance" help="If specified, paired-end distance will be checked when assigning fragments to meta-features or features. This option is only applicable when -p (Count fragments instead of reads) is specified. The distance thresholds can be specified using -d and -D (minimum and maximum fragment/template length) options."> |
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236 <option value="true">Check the distance between paired reads</option> |
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237 <option value="false" selected="True">Do not check</option> |
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238 </param> |
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239 <when value="true"> |
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240 <param name="minimum_fragment_length" |
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241 type="integer" |
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242 value="50" |
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243 min="0" |
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244 argument="-d" |
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245 label="Minimum fragment/template length."/> |
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246 <param name="maximum_fragment_length" |
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247 type="integer" |
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248 value="600" |
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249 min="1" |
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250 argument="-D" |
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251 label="Maximum fragment/template length."/> |
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252 </when> |
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253 <when value="false"/> |
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254 </conditional> |
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255 |
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256 <param name="only_both_ends" |
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257 type="boolean" |
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258 truevalue=" -B" |
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259 falsevalue="" |
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260 argument="-B" |
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261 label="Only allow fragments with both reads aligned" |
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262 help="If specified, only fragments that have both ends successfully aligned will be considered for summarization. This option is only applicable for paired-end reads."/> |
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8
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263 |
|
32
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264 <param name="exclude_chimerics" |
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265 type="boolean" |
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266 truevalue=" -C" |
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267 falsevalue="" |
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268 argument="-C" |
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269 checked="true" |
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270 label="Exclude chimeric fragments" |
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271 help="If specified, the chimeric fragments (those fragments that have their two ends aligned to different chromosomes) will NOT be included for summarization. This option is only applicable for paired-end read data."/> |
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272 </when> |
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273 </conditional> |
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8
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274 |
|
31
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275 <section name="read_filtering_parameters" title="Read filtering options"> |
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276 <param name="mapping_quality" |
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277 type="integer" |
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278 value="0" |
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32
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279 min="0" |
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31
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280 argument="-Q" |
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281 label="Minimum mapping quality per read" |
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282 help="The minimum mapping quality score a read must satisfy in order to be counted. For paired-end reads, at least one end should satisfy this criteria. 0 by default."/> |
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31
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283 <param name="splitonly" type="select" display="radio" label="Filter split alignments" help="Split alignments are alignments with CIGAR string containing 'N', e.g. exon spanning reads in RNASeq."> |
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284 <option value="">No filtering: count split and non-split alignments</option> |
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285 <option value="--splitOnly">Count only split alignments (--splitOnly)</option> |
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286 <option value="--nonSplitOnly">Count only non-split alignments (--nonSplitOnly)</option> |
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287 </param> |
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288 <param type="boolean" |
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289 truevalue=" --primary" |
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290 falsevalue="" |
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291 argument="--primary" |
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292 label="Only count primary alignments" |
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293 help="If specified, only primary alignments will be counted. Primary and secondary alignments are identified using bit 0x100 in theFlag field of SAM/BAM files. All primary alignments in a dataset will be counted regardless of whether they are from multi-mapping reads or not ('-M' is ignored)."/> |
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31
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294 <param name="ignore_dup" |
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295 type="boolean" |
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296 truevalue=" --ignoreDup" |
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297 falsevalue="" |
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298 argument="--ignoreDup" |
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299 label="Ignore reads marked as duplicate" |
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300 help="If specified, reads that were marked as duplicates will be ignored. Bit Ox400 in the FLAG field of a SAM/BAM file is used for identifying duplicate reads. In paired end data, the entire read pair will be ignored if at least one end is found to be a duplicate read."/> |
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31
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301 </section> |
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302 |
|
0
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303 <section name="extended_parameters" title="Advanced options"> |
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26
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304 <conditional name = "multifeatures"> |
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305 <param name="multifeat" type="select" label="Allow reads to map to multiple features" help="Setting -O, -M and --fraction"> |
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31
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306 <option value="" selected="true">Disabled: reads that align to multiple features or overlapping features are excluded</option> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 839f962c859728f53bb696cea0720862418f1a13"
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307 <option value="-M">Enabled: multi-mapping reads are included (-M)</option> |
|
26
48a5ee6c175c
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit d80704d28a7be0c42d41e48f8e12b9d727233be4"
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308 <option value="-O">Enabled: multi-overlapping features are included (-O)</option> |
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48a5ee6c175c
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit d80704d28a7be0c42d41e48f8e12b9d727233be4"
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309 <option value="-O -M">Enabled: both multi-mapping and multi-overlapping features are included (-M -O)</option> |
|
0
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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changeset
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310 </param> |
|
26
48a5ee6c175c
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit d80704d28a7be0c42d41e48f8e12b9d727233be4"
iuc
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311 <when value=""/> |
|
48a5ee6c175c
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit d80704d28a7be0c42d41e48f8e12b9d727233be4"
iuc
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312 <when value="-M"> |
|
32
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313 <param type="boolean" |
|
26
48a5ee6c175c
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit d80704d28a7be0c42d41e48f8e12b9d727233be4"
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314 truevalue="--fraction" |
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48a5ee6c175c
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit d80704d28a7be0c42d41e48f8e12b9d727233be4"
iuc
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315 falsevalue="" |
|
48a5ee6c175c
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit d80704d28a7be0c42d41e48f8e12b9d727233be4"
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316 argument="--fraction" |
|
31
83ab9e468b86
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 839f962c859728f53bb696cea0720862418f1a13"
iuc
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changeset
|
317 label="Assign fractions to multi-mapping reads" |
|
83ab9e468b86
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 839f962c859728f53bb696cea0720862418f1a13"
iuc
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318 help="If specified, a fractional count 1/x will be generated for each multi-mapping read, where x is the number of alignments (indicated by 'NH' tag) reported for the read."/> |
|
0
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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changeset
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319 </when> |
|
26
48a5ee6c175c
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit d80704d28a7be0c42d41e48f8e12b9d727233be4"
iuc
parents:
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changeset
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320 <when value="-O"> |
|
32
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents:
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|
321 <param type="boolean" |
|
26
48a5ee6c175c
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit d80704d28a7be0c42d41e48f8e12b9d727233be4"
iuc
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changeset
|
322 truevalue="--fraction" |
|
48a5ee6c175c
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit d80704d28a7be0c42d41e48f8e12b9d727233be4"
iuc
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changeset
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323 falsevalue="" |
|
48a5ee6c175c
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit d80704d28a7be0c42d41e48f8e12b9d727233be4"
iuc
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changeset
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324 argument="--fraction" |
|
31
83ab9e468b86
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 839f962c859728f53bb696cea0720862418f1a13"
iuc
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diff
changeset
|
325 label="Assign fractions to multi-overlapping features" |
|
83ab9e468b86
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 839f962c859728f53bb696cea0720862418f1a13"
iuc
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changeset
|
326 help="If specified, a fractional count 1/y will be generated for each multi-overlapping feature, where y is the number of features overlapping with the read."/> |
|
26
48a5ee6c175c
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit d80704d28a7be0c42d41e48f8e12b9d727233be4"
iuc
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|
327 </when> |
|
48a5ee6c175c
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit d80704d28a7be0c42d41e48f8e12b9d727233be4"
iuc
parents:
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328 <when value="-O -M"> |
|
32
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
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329 <param type="boolean" |
|
26
48a5ee6c175c
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit d80704d28a7be0c42d41e48f8e12b9d727233be4"
iuc
parents:
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changeset
|
330 truevalue="--fraction" |
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48a5ee6c175c
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit d80704d28a7be0c42d41e48f8e12b9d727233be4"
iuc
parents:
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331 falsevalue="" |
|
48a5ee6c175c
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit d80704d28a7be0c42d41e48f8e12b9d727233be4"
iuc
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332 argument="--fraction" |
|
31
83ab9e468b86
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 839f962c859728f53bb696cea0720862418f1a13"
iuc
parents:
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diff
changeset
|
333 label="Assign fractions to both multi-mapping reads and multi-overlapping features" |
|
83ab9e468b86
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 839f962c859728f53bb696cea0720862418f1a13"
iuc
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334 help="If specified, a fractional count 1/(x*y) will be generated, where x is the number of alignments (indicated by 'NH' tag) and y the number of overlapping features."/> |
|
26
48a5ee6c175c
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit d80704d28a7be0c42d41e48f8e12b9d727233be4"
iuc
parents:
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changeset
|
335 </when> |
|
0
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
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336 </conditional> |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
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changeset
|
337 |
|
6
e70afb590e50
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit ffb40a055701dca74e29e651dd820a6bda3d844b
iuc
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338 <conditional name="exon_exon_junction_read_counting_enabled"> |
|
32
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339 <param name="count_exon_exon_junction_reads" type="select" argument="-J" label="Exon-exon junctions" help="Junctions are identified from those exon-spanning reads (containing ‘N’ in CIGAR string) in input data. The output result includes names of primary and secondary genes that overlap at least one of the two splice sites of a junction."> |
|
456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
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340 <option value="-J">Count reads supporting each exon-exon junction.</option> |
|
456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
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341 <option value="" selected="True">Do not count</option> |
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456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
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342 </param> |
|
6
e70afb590e50
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit ffb40a055701dca74e29e651dd820a6bda3d844b
iuc
parents:
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changeset
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343 <when value="-J"> |
|
7
889d2cd8a809
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a705dfd329f2e917d549215715385f5ef5001d17
iuc
parents:
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diff
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|
344 <param name="genome" argument="-G" type="data" format="fasta" optional="true" |
|
889d2cd8a809
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a705dfd329f2e917d549215715385f5ef5001d17
iuc
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345 label="Reference sequence file" |
|
32
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
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diff
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|
346 help="The FASTA-format file that contains the reference sequences used in read mapping can be used to improve read counting for junctions"/> |
|
6
e70afb590e50
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit ffb40a055701dca74e29e651dd820a6bda3d844b
iuc
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347 </when> |
|
32
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
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|
348 <when value=""/> |
|
6
e70afb590e50
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit ffb40a055701dca74e29e651dd820a6bda3d844b
iuc
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349 </conditional> |
|
e70afb590e50
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit ffb40a055701dca74e29e651dd820a6bda3d844b
iuc
parents:
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changeset
|
350 |
|
e70afb590e50
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit ffb40a055701dca74e29e651dd820a6bda3d844b
iuc
parents:
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diff
changeset
|
351 <param name="long_reads" argument="-L" type="boolean" truevalue="-L" falsevalue="" |
|
e70afb590e50
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit ffb40a055701dca74e29e651dd820a6bda3d844b
iuc
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352 label="Long reads" |
|
32
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353 help="If specified, long reads such as Nanopore and PacBio reads will be counted. Long read counting can only run in one thread and only reads (not read-pairs) can be counted."/> |
|
6
e70afb590e50
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit ffb40a055701dca74e29e651dd820a6bda3d844b
iuc
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|
354 |
|
10
7d1e37c6ef85
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
iuc
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diff
changeset
|
355 <param name="by_read_group" argument="--byReadGroup" type="boolean" truevalue="--byReadGroup" falsevalue="" |
|
8
bff059ad4d13
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2228563ac1c2bb236c8ac83d08d2b86e21a39e41
iuc
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changeset
|
356 label="Count reads by read group" |
|
32
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
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|
357 help="If specified, reads are counted for each read group separately. The 'RG' tag must be present in the input BAM/SAM alignment files."/> |
|
8
bff059ad4d13
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2228563ac1c2bb236c8ac83d08d2b86e21a39e41
iuc
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|
358 |
|
0
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
359 <param name="largest_overlap" |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
360 type="boolean" |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
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changeset
|
361 truevalue=" --largestOverlap" |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
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changeset
|
362 falsevalue="" |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
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changeset
|
363 argument="--largestOverlap" |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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changeset
|
364 label="Largest overlap" |
|
32
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365 help="If specified, reads (or fragments) will be assigned to the target that has the largest number of overlapping bases"/> |
|
0
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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changeset
|
366 |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
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changeset
|
367 <param name="min_overlap" |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
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changeset
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368 type="integer" |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
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changeset
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369 value="1" |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
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changeset
|
370 argument="--minOverlap" |
|
8
bff059ad4d13
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2228563ac1c2bb236c8ac83d08d2b86e21a39e41
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371 label="Minimum bases of overlap" |
|
32
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372 help="Specify the minimum required number of overlapping bases between a read (or a fragment) and a feature. 1 by default. If a negative value is provided, the read will be extended from both ends."/> |
|
0
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
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changeset
|
373 |
|
10
7d1e37c6ef85
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
iuc
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|
374 <param name="frac_overlap" |
|
8
bff059ad4d13
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2228563ac1c2bb236c8ac83d08d2b86e21a39e41
iuc
parents:
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diff
changeset
|
375 type="integer" |
|
bff059ad4d13
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2228563ac1c2bb236c8ac83d08d2b86e21a39e41
iuc
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changeset
|
376 value="0" |
|
bff059ad4d13
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2228563ac1c2bb236c8ac83d08d2b86e21a39e41
iuc
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changeset
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377 min="0" |
|
bff059ad4d13
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2228563ac1c2bb236c8ac83d08d2b86e21a39e41
iuc
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changeset
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378 max="1" |
|
bff059ad4d13
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2228563ac1c2bb236c8ac83d08d2b86e21a39e41
iuc
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changeset
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379 argument="--fracOverlap" |
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bff059ad4d13
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2228563ac1c2bb236c8ac83d08d2b86e21a39e41
iuc
parents:
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changeset
|
380 label="Minimum fraction (of read) overlapping a feature" |
|
32
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381 help="Specify the minimum required fraction of overlapping bases between a read (or a fragment) and a feature. Value should be within range [0,1]. 0 by default. Number of overlapping bases is counted from both reads if paired end. Both this option and '--minOverlap' need to be satisfied for read assignment."/> |
|
8
bff059ad4d13
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2228563ac1c2bb236c8ac83d08d2b86e21a39e41
iuc
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|
382 |
|
10
7d1e37c6ef85
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
iuc
parents:
9
diff
changeset
|
383 <param name="frac_overlap_feature" |
|
8
bff059ad4d13
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2228563ac1c2bb236c8ac83d08d2b86e21a39e41
iuc
parents:
7
diff
changeset
|
384 type="integer" |
|
bff059ad4d13
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2228563ac1c2bb236c8ac83d08d2b86e21a39e41
iuc
parents:
7
diff
changeset
|
385 value="0" |
|
bff059ad4d13
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2228563ac1c2bb236c8ac83d08d2b86e21a39e41
iuc
parents:
7
diff
changeset
|
386 min="0" |
|
bff059ad4d13
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2228563ac1c2bb236c8ac83d08d2b86e21a39e41
iuc
parents:
7
diff
changeset
|
387 max="1" |
|
bff059ad4d13
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2228563ac1c2bb236c8ac83d08d2b86e21a39e41
iuc
parents:
7
diff
changeset
|
388 argument="--fracOverlapFeature" |
|
bff059ad4d13
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2228563ac1c2bb236c8ac83d08d2b86e21a39e41
iuc
parents:
7
diff
changeset
|
389 label="Minimum fraction (of feature) overlapping a read" |
|
32
456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents:
31
diff
changeset
|
390 help="Specify the minimum required fraction of bases included in a feature overlapping bases between a read (or a read-pair). Value should be within range [0,1]. 0 by default."/> |
|
8
bff059ad4d13
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2228563ac1c2bb236c8ac83d08d2b86e21a39e41
iuc
parents:
7
diff
changeset
|
391 |
|
0
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
392 <param name="read_extension_5p" |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
393 type="integer" |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
394 value="0" |
|
32
456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents:
31
diff
changeset
|
395 min="0" |
|
0
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
396 argument="--readExtension5" |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
397 label="Read 5' extension" |
|
32
456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents:
31
diff
changeset
|
398 help="Reads are extended upstream by ... bases from their 5' end"/> |
|
0
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
399 |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
400 <param name="read_extension_3p" |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
401 type="integer" |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
402 value="0" |
|
32
456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents:
31
diff
changeset
|
403 min="0" |
|
0
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
404 argument="--readExtension3" |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
405 label="Read 3' extension" |
|
32
456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents:
31
diff
changeset
|
406 help="Reads are extended upstream by ... bases from their 3' end"/> |
|
0
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
407 |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
408 <param name="read_reduction" |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
409 type="select" |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
410 label="Reduce read to single position" |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
411 argument="--read2pos" |
|
3
711c80e57384
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 1c0d28b6cefe154e8cf037c9f36200e8f52a838f
iuc
parents:
2
diff
changeset
|
412 help="The read is reduced to its 5' most base or 3'most base. Read summarization is then performed based on the single base the the read is reduced to."> |
|
0
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
413 <option value="" selected="true">Leave the read as it is</option> |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
414 <option value="--read2pos 5">Reduce it to the 5' end</option> |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
415 <option value="--read2pos 3">Reduce it to the 3' end</option> |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
416 </param> |
|
8
bff059ad4d13
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2228563ac1c2bb236c8ac83d08d2b86e21a39e41
iuc
parents:
7
diff
changeset
|
417 |
|
17
5c20eb47f759
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 6c859db1a131f3f17aecdf44af80ad93b0e9c018
iuc
parents:
15
diff
changeset
|
418 <param type="boolean" |
|
5c20eb47f759
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 6c859db1a131f3f17aecdf44af80ad93b0e9c018
iuc
parents:
15
diff
changeset
|
419 truevalue="-R BAM" |
|
5c20eb47f759
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 6c859db1a131f3f17aecdf44af80ad93b0e9c018
iuc
parents:
15
diff
changeset
|
420 falsevalue="" |
|
5c20eb47f759
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 6c859db1a131f3f17aecdf44af80ad93b0e9c018
iuc
parents:
15
diff
changeset
|
421 argument="-R" |
|
5c20eb47f759
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 6c859db1a131f3f17aecdf44af80ad93b0e9c018
iuc
parents:
15
diff
changeset
|
422 label="Annotates the alignment file with 'XS:Z:'-tags to described per read or read-pair the corresponding assigned feature(s)." |
|
32
456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents:
31
diff
changeset
|
423 help=""/> |
|
17
5c20eb47f759
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 6c859db1a131f3f17aecdf44af80ad93b0e9c018
iuc
parents:
15
diff
changeset
|
424 |
|
0
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
425 </section> |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
426 </inputs> |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
427 <outputs> |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
428 <data format="tabular" |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
429 name="output_medium" |
|
20
b4f3420c2a70
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit c8c08f052bdffcbfd14eebd760af923486bcf8c5
iuc
parents:
19
diff
changeset
|
430 label="${tool.name} on ${on_string}: Counts (with length)"> |
|
0
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
431 <filter>format == "tabdel_medium"</filter> |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
432 <actions> |
|
32
456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents:
31
diff
changeset
|
433 <action name="column_names" type="metadata" default="Geneid,${alignment.element_identifier},Length"/> |
|
0
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
434 </actions> |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
435 </data> |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
436 |
|
17
5c20eb47f759
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 6c859db1a131f3f17aecdf44af80ad93b0e9c018
iuc
parents:
15
diff
changeset
|
437 <data format="bam" |
|
5c20eb47f759
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 6c859db1a131f3f17aecdf44af80ad93b0e9c018
iuc
parents:
15
diff
changeset
|
438 name="output_bam" |
|
5c20eb47f759
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 6c859db1a131f3f17aecdf44af80ad93b0e9c018
iuc
parents:
15
diff
changeset
|
439 label="${tool.name} on ${on_string}: Alignment file"> |
|
5c20eb47f759
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 6c859db1a131f3f17aecdf44af80ad93b0e9c018
iuc
parents:
15
diff
changeset
|
440 <filter>extended_parameters['R']</filter> |
|
5c20eb47f759
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 6c859db1a131f3f17aecdf44af80ad93b0e9c018
iuc
parents:
15
diff
changeset
|
441 </data> |
|
5c20eb47f759
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 6c859db1a131f3f17aecdf44af80ad93b0e9c018
iuc
parents:
15
diff
changeset
|
442 |
|
0
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
443 <data format="tabular" |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
444 name="output_short" |
|
20
b4f3420c2a70
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit c8c08f052bdffcbfd14eebd760af923486bcf8c5
iuc
parents:
19
diff
changeset
|
445 label="${tool.name} on ${on_string}: Counts"> |
|
13
c7085a3028ef
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2bd06c2b43c295fb4cf172c4f156fed5475855a4
iuc
parents:
12
diff
changeset
|
446 <filter>format == "tabdel_short"</filter> |
|
0
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
447 <actions> |
|
32
456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents:
31
diff
changeset
|
448 <action name="column_names" type="metadata" default="Geneid,${alignment.element_identifier}"/> |
|
0
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
449 </actions> |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
450 </data> |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
451 |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
452 <data format="tabular" |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
453 name="output_full" |
|
20
b4f3420c2a70
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit c8c08f052bdffcbfd14eebd760af923486bcf8c5
iuc
parents:
19
diff
changeset
|
454 label="${tool.name} on ${on_string}: Counts (with location)"> |
|
0
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
455 <filter>format == "tabdel_full"</filter> |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
456 <actions> |
|
32
456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents:
31
diff
changeset
|
457 <action name="column_names" type="metadata" default="Geneid,Chr,Start,End,Strand,Length,${alignment.element_identifier}"/> |
|
0
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
458 </actions> |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
459 </data> |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
460 |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
461 <data format="tabular" |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
462 name="output_summary" |
|
20
b4f3420c2a70
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit c8c08f052bdffcbfd14eebd760af923486bcf8c5
iuc
parents:
19
diff
changeset
|
463 label="${tool.name} on ${on_string}: Summary"> |
|
0
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
464 <actions> |
|
32
456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents:
31
diff
changeset
|
465 <action name="column_names" type="metadata" default="Status,${alignment.element_identifier}"/> |
|
0
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
466 </actions> |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
467 </data> |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
468 |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
469 <data format="tabular" |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
470 name="output_feature_lengths" |
|
20
b4f3420c2a70
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit c8c08f052bdffcbfd14eebd760af923486bcf8c5
iuc
parents:
19
diff
changeset
|
471 label="${tool.name} on ${on_string}: Feature lengths"> |
|
6
e70afb590e50
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit ffb40a055701dca74e29e651dd820a6bda3d844b
iuc
parents:
4
diff
changeset
|
472 <filter>include_feature_length_file</filter> |
|
0
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
473 <actions> |
|
32
456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents:
31
diff
changeset
|
474 <action name="column_names" type="metadata" default="Feature,Length"/> |
|
0
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
475 </actions> |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
476 </data> |
|
6
e70afb590e50
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit ffb40a055701dca74e29e651dd820a6bda3d844b
iuc
parents:
4
diff
changeset
|
477 |
|
e70afb590e50
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit ffb40a055701dca74e29e651dd820a6bda3d844b
iuc
parents:
4
diff
changeset
|
478 <data name="output_jcounts" format="tabular" |
|
20
b4f3420c2a70
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit c8c08f052bdffcbfd14eebd760af923486bcf8c5
iuc
parents:
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diff
changeset
|
479 label="${tool.name} on ${on_string}: Junction counts"> |
|
7
889d2cd8a809
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a705dfd329f2e917d549215715385f5ef5001d17
iuc
parents:
6
diff
changeset
|
480 <filter>extended_parameters['exon_exon_junction_read_counting_enabled']['count_exon_exon_junction_reads']</filter> |
|
6
e70afb590e50
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit ffb40a055701dca74e29e651dd820a6bda3d844b
iuc
parents:
4
diff
changeset
|
481 <actions> |
|
10
7d1e37c6ef85
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
iuc
parents:
9
diff
changeset
|
482 <action name="column_names" type="metadata" |
|
32
456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents:
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diff
changeset
|
483 default="PrimaryGene,SecondaryGene,Site1_chr,Site1_location,Site1_strand,Site2_chr,Site2_location,Site2_strand,${alignment.element_identifier}"/> |
|
6
e70afb590e50
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit ffb40a055701dca74e29e651dd820a6bda3d844b
iuc
parents:
4
diff
changeset
|
484 </actions> |
|
e70afb590e50
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit ffb40a055701dca74e29e651dd820a6bda3d844b
iuc
parents:
4
diff
changeset
|
485 </data> |
|
0
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
486 </outputs> |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
487 <tests> |
|
7
889d2cd8a809
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a705dfd329f2e917d549215715385f5ef5001d17
iuc
parents:
6
diff
changeset
|
488 <test expect_num_outputs="3"> |
|
34
12b3317716d3
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit fc2a03ae0ef4d1d0d5f2f05a6ecc0fc4812931e1
iuc
parents:
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diff
changeset
|
489 <param name="alignment" value="featureCounts_input1.bam" ftype="unsorted.bam" dbkey="hg38"/> |
|
11
d0beb2753aba
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 4c095ddb0f2c369d2bf3fc5ca386f6981a2fa0eb
iuc
parents:
10
diff
changeset
|
490 <param name="anno_select" value="history"/> |
|
32
456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents:
31
diff
changeset
|
491 <param name="reference_gene_sets" value="featureCounts_guide.gff" ftype="gff" dbkey="hg38"/> |
|
456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents:
31
diff
changeset
|
492 <param name="format" value="tabdel_medium"/> |
|
0
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
493 <param name="include_feature_length_file" value="true"/> |
|
4
2451333b004b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 57dfb61ba50ecfbe8f1c624fdbe2d0a3a8deaa04
iuc
parents:
3
diff
changeset
|
494 <output name="output_medium" file="output_1_medium.tab"> |
|
2451333b004b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 57dfb61ba50ecfbe8f1c624fdbe2d0a3a8deaa04
iuc
parents:
3
diff
changeset
|
495 <metadata name="column_names" value="Geneid,featureCounts_input1.bam,Length"/> |
|
2451333b004b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 57dfb61ba50ecfbe8f1c624fdbe2d0a3a8deaa04
iuc
parents:
3
diff
changeset
|
496 </output> |
|
2451333b004b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 57dfb61ba50ecfbe8f1c624fdbe2d0a3a8deaa04
iuc
parents:
3
diff
changeset
|
497 <output name="output_summary" file="output_1_summary.tab"> |
|
2451333b004b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 57dfb61ba50ecfbe8f1c624fdbe2d0a3a8deaa04
iuc
parents:
3
diff
changeset
|
498 <metadata name="column_names" value="Status,featureCounts_input1.bam"/> |
|
2451333b004b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 57dfb61ba50ecfbe8f1c624fdbe2d0a3a8deaa04
iuc
parents:
3
diff
changeset
|
499 </output> |
|
0
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
500 </test> |
|
7
889d2cd8a809
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a705dfd329f2e917d549215715385f5ef5001d17
iuc
parents:
6
diff
changeset
|
501 <test expect_num_outputs="3"> |
|
34
12b3317716d3
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit fc2a03ae0ef4d1d0d5f2f05a6ecc0fc4812931e1
iuc
parents:
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diff
changeset
|
502 <param name="alignment" value="featureCounts_input1.bam" ftype="unsorted.bam" dbkey="hg38"/> |
|
11
d0beb2753aba
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 4c095ddb0f2c369d2bf3fc5ca386f6981a2fa0eb
iuc
parents:
10
diff
changeset
|
503 <param name="anno_select" value="history"/> |
|
32
456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents:
31
diff
changeset
|
504 <param name="reference_gene_sets" value="featureCounts_guide.gff" ftype="gff" dbkey="hg38"/> |
|
456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents:
31
diff
changeset
|
505 <param name="format" value="tabdel_full"/> |
|
0
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
506 <param name="include_feature_length_file" value="true"/> |
|
4
2451333b004b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 57dfb61ba50ecfbe8f1c624fdbe2d0a3a8deaa04
iuc
parents:
3
diff
changeset
|
507 <output name="output_full" file="output_1_full.tab"> |
|
2451333b004b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 57dfb61ba50ecfbe8f1c624fdbe2d0a3a8deaa04
iuc
parents:
3
diff
changeset
|
508 <metadata name="column_names" value="Geneid,Chr,Start,End,Strand,Length,featureCounts_input1.bam"/> |
|
2451333b004b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 57dfb61ba50ecfbe8f1c624fdbe2d0a3a8deaa04
iuc
parents:
3
diff
changeset
|
509 </output> |
|
2451333b004b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 57dfb61ba50ecfbe8f1c624fdbe2d0a3a8deaa04
iuc
parents:
3
diff
changeset
|
510 <output name="output_summary" file="output_1_summary.tab"> |
|
2451333b004b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 57dfb61ba50ecfbe8f1c624fdbe2d0a3a8deaa04
iuc
parents:
3
diff
changeset
|
511 <metadata name="column_names" value="Status,featureCounts_input1.bam"/> |
|
2451333b004b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 57dfb61ba50ecfbe8f1c624fdbe2d0a3a8deaa04
iuc
parents:
3
diff
changeset
|
512 </output> |
|
2451333b004b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 57dfb61ba50ecfbe8f1c624fdbe2d0a3a8deaa04
iuc
parents:
3
diff
changeset
|
513 <output name="output_feature_lengths" file="output_feature_lengths.tab"> |
|
2451333b004b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 57dfb61ba50ecfbe8f1c624fdbe2d0a3a8deaa04
iuc
parents:
3
diff
changeset
|
514 <metadata name="column_names" value="Feature,Length"/> |
|
2451333b004b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 57dfb61ba50ecfbe8f1c624fdbe2d0a3a8deaa04
iuc
parents:
3
diff
changeset
|
515 </output> |
|
0
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff
changeset
|
516 </test> |
|
10
7d1e37c6ef85
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
iuc
parents:
9
diff
changeset
|
517 <test expect_num_outputs="4"> |
|
34
12b3317716d3
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit fc2a03ae0ef4d1d0d5f2f05a6ecc0fc4812931e1
iuc
parents:
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diff
changeset
|
518 <param name="alignment" value="featureCounts_input1.bam" ftype="unsorted.bam" dbkey="hg38"/> |
|
11
d0beb2753aba
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 4c095ddb0f2c369d2bf3fc5ca386f6981a2fa0eb
iuc
parents:
10
diff
changeset
|
519 <param name="anno_select" value="history"/> |
|
32
456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents:
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diff
changeset
|
520 <param name="reference_gene_sets" value="featureCounts_guide.gff" ftype="gff" dbkey="hg38"/> |
|
456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents:
31
diff
changeset
|
521 <param name="format" value="tabdel_short"/> |
|
10
7d1e37c6ef85
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
iuc
parents:
9
diff
changeset
|
522 <param name="include_feature_length_file" value="true"/> |
|
7d1e37c6ef85
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
iuc
parents:
9
diff
changeset
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523 <param name="count_exon_exon_junction_reads" value="-J"/> |
|
13
c7085a3028ef
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2bd06c2b43c295fb4cf172c4f156fed5475855a4
iuc
parents:
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diff
changeset
|
524 <output name="output_short" file="output_1_short.tab"> |
|
10
7d1e37c6ef85
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
iuc
parents:
9
diff
changeset
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525 <metadata name="column_names" value="Geneid,featureCounts_input1.bam"/> |
|
7d1e37c6ef85
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
iuc
parents:
9
diff
changeset
|
526 </output> |
|
13
c7085a3028ef
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2bd06c2b43c295fb4cf172c4f156fed5475855a4
iuc
parents:
12
diff
changeset
|
527 <output name="output_summary" file="output_1_summary.tab"> |
|
10
7d1e37c6ef85
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
iuc
parents:
9
diff
changeset
|
528 <metadata name="column_names" value="Status,featureCounts_input1.bam"/> |
|
7d1e37c6ef85
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
iuc
parents:
9
diff
changeset
|
529 </output> |
|
13
c7085a3028ef
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2bd06c2b43c295fb4cf172c4f156fed5475855a4
iuc
parents:
12
diff
changeset
|
530 <output name="output_jcounts" file="output_1_jcounts.tab"> |
|
10
7d1e37c6ef85
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
iuc
parents:
9
diff
changeset
|
531 <metadata name="column_names" value="PrimaryGene,SecondaryGene,Site1_chr,Site1_location,Site1_strand,Site2_chr,Site2_location,Site2_strand,featureCounts_input1.bam"/> |
|
7d1e37c6ef85
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
iuc
parents:
9
diff
changeset
|
532 </output> |
|
7d1e37c6ef85
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
iuc
parents:
9
diff
changeset
|
533 </test> |
|
11
d0beb2753aba
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 4c095ddb0f2c369d2bf3fc5ca386f6981a2fa0eb
iuc
parents:
10
diff
changeset
|
534 <!-- Ensure featureCounts built-in annotation works --> |
|
17
5c20eb47f759
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 6c859db1a131f3f17aecdf44af80ad93b0e9c018
iuc
parents:
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diff
changeset
|
535 <test expect_num_outputs="3"> |
|
34
12b3317716d3
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit fc2a03ae0ef4d1d0d5f2f05a6ecc0fc4812931e1
iuc
parents:
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diff
changeset
|
536 <param name="alignment" value="pairend_strandspecific_51mer_hg19_chr1_1-100000.bam" ftype="unsorted.bam" dbkey="hg19"/> |
|
10
7d1e37c6ef85
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
iuc
parents:
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diff
changeset
|
537 <param name="anno_select" value="builtin"/> |
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32
456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents:
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diff
changeset
|
538 <param name="format" value="tabdel_short"/> |
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456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents:
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diff
changeset
|
539 <conditional name="pe_parameters"> |
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456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents:
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diff
changeset
|
540 <param name="paired_end_status" value="PE_individual"/> |
|
456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents:
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diff
changeset
|
541 </conditional> |
|
17
5c20eb47f759
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 6c859db1a131f3f17aecdf44af80ad93b0e9c018
iuc
parents:
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diff
changeset
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542 <section name="extended_parameters"> |
|
32
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents:
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diff
changeset
|
543 <param name="R" value="true"/> |
|
17
5c20eb47f759
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 6c859db1a131f3f17aecdf44af80ad93b0e9c018
iuc
parents:
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diff
changeset
|
544 </section> |
|
10
7d1e37c6ef85
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
iuc
parents:
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diff
changeset
|
545 <output name="output_short" file="output_builtin_hg19.tab"> |
|
7d1e37c6ef85
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
iuc
parents:
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diff
changeset
|
546 <metadata name="column_names" value="Geneid,pairend_strandspecific_51mer_hg19_chr1_1-100000.bam"/> |
|
7d1e37c6ef85
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
iuc
parents:
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diff
changeset
|
547 </output> |
|
7d1e37c6ef85
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
iuc
parents:
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diff
changeset
|
548 <output name="output_summary" file="output_summary_builtin_hg19.tab"/> |
|
17
5c20eb47f759
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 6c859db1a131f3f17aecdf44af80ad93b0e9c018
iuc
parents:
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diff
changeset
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549 <output name="output_bam" file="output.bam" ftype="bam"/> |
|
10
7d1e37c6ef85
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
iuc
parents:
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diff
changeset
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550 </test> |
|
33
dad3302e7b34
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a0d90afa3160e737786cc448aaf7c92df94a64cc
iuc
parents:
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diff
changeset
|
551 <!-- Ensure fragment counting works --> |
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dad3302e7b34
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a0d90afa3160e737786cc448aaf7c92df94a64cc
iuc
parents:
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diff
changeset
|
552 <test expect_num_outputs="3"> |
|
34
12b3317716d3
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit fc2a03ae0ef4d1d0d5f2f05a6ecc0fc4812931e1
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parents:
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553 <param name="alignment" value="pairend_strandspecific_51mer_hg19_chr1_1-100000.bam" ftype="unsorted.bam" dbkey="hg19"/> |
|
33
dad3302e7b34
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a0d90afa3160e737786cc448aaf7c92df94a64cc
iuc
parents:
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diff
changeset
|
554 <param name="anno_select" value="builtin"/> |
|
dad3302e7b34
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a0d90afa3160e737786cc448aaf7c92df94a64cc
iuc
parents:
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diff
changeset
|
555 <param name="format" value="tabdel_short"/> |
|
dad3302e7b34
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a0d90afa3160e737786cc448aaf7c92df94a64cc
iuc
parents:
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diff
changeset
|
556 <conditional name="pe_parameters"> |
|
dad3302e7b34
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a0d90afa3160e737786cc448aaf7c92df94a64cc
iuc
parents:
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diff
changeset
|
557 <param name="paired_end_status" value="PE_fragments"/> |
|
dad3302e7b34
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a0d90afa3160e737786cc448aaf7c92df94a64cc
iuc
parents:
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diff
changeset
|
558 </conditional> |
|
dad3302e7b34
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a0d90afa3160e737786cc448aaf7c92df94a64cc
iuc
parents:
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diff
changeset
|
559 <section name="extended_parameters"> |
|
dad3302e7b34
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a0d90afa3160e737786cc448aaf7c92df94a64cc
iuc
parents:
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diff
changeset
|
560 <param name="R" value="true"/> |
|
dad3302e7b34
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a0d90afa3160e737786cc448aaf7c92df94a64cc
iuc
parents:
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diff
changeset
|
561 </section> |
|
dad3302e7b34
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a0d90afa3160e737786cc448aaf7c92df94a64cc
iuc
parents:
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diff
changeset
|
562 <output name="output_short" file="output_builtin_hg19_fragment.tab"> |
|
dad3302e7b34
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a0d90afa3160e737786cc448aaf7c92df94a64cc
iuc
parents:
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diff
changeset
|
563 <metadata name="column_names" value="Geneid,pairend_strandspecific_51mer_hg19_chr1_1-100000.bam"/> |
|
dad3302e7b34
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a0d90afa3160e737786cc448aaf7c92df94a64cc
iuc
parents:
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diff
changeset
|
564 </output> |
|
dad3302e7b34
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a0d90afa3160e737786cc448aaf7c92df94a64cc
iuc
parents:
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diff
changeset
|
565 <output name="output_summary" file="output_summary_builtin_hg19_fragments.tab"/> |
|
dad3302e7b34
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a0d90afa3160e737786cc448aaf7c92df94a64cc
iuc
parents:
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diff
changeset
|
566 <output name="output_bam" file="output_fragments.bam" ftype="bam"/> |
|
dad3302e7b34
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a0d90afa3160e737786cc448aaf7c92df94a64cc
iuc
parents:
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diff
changeset
|
567 </test> |
|
11
d0beb2753aba
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 4c095ddb0f2c369d2bf3fc5ca386f6981a2fa0eb
iuc
parents:
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diff
changeset
|
568 <!-- Ensure cached GTFs work --> |
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d0beb2753aba
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 4c095ddb0f2c369d2bf3fc5ca386f6981a2fa0eb
iuc
parents:
10
diff
changeset
|
569 <test expect_num_outputs="3"> |
|
34
12b3317716d3
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit fc2a03ae0ef4d1d0d5f2f05a6ecc0fc4812931e1
iuc
parents:
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diff
changeset
|
570 <param name="alignment" value="featureCounts_input1.bam" ftype="unsorted.bam" dbkey="hg38"/> |
|
11
d0beb2753aba
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 4c095ddb0f2c369d2bf3fc5ca386f6981a2fa0eb
iuc
parents:
10
diff
changeset
|
571 <param name="anno_select" value="cached"/> |
|
32
456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents:
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diff
changeset
|
572 <param name="format" value="tabdel_medium"/> |
|
11
d0beb2753aba
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 4c095ddb0f2c369d2bf3fc5ca386f6981a2fa0eb
iuc
parents:
10
diff
changeset
|
573 <param name="include_feature_length_file" value="true"/> |
|
d0beb2753aba
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 4c095ddb0f2c369d2bf3fc5ca386f6981a2fa0eb
iuc
parents:
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diff
changeset
|
574 <output name="output_medium" file="output_1_medium.tab"> |
|
d0beb2753aba
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 4c095ddb0f2c369d2bf3fc5ca386f6981a2fa0eb
iuc
parents:
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diff
changeset
|
575 <metadata name="column_names" value="Geneid,featureCounts_input1.bam,Length"/> |
|
d0beb2753aba
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 4c095ddb0f2c369d2bf3fc5ca386f6981a2fa0eb
iuc
parents:
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diff
changeset
|
576 </output> |
|
d0beb2753aba
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 4c095ddb0f2c369d2bf3fc5ca386f6981a2fa0eb
iuc
parents:
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diff
changeset
|
577 <output name="output_summary" file="output_1_summary.tab"> |
|
d0beb2753aba
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 4c095ddb0f2c369d2bf3fc5ca386f6981a2fa0eb
iuc
parents:
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diff
changeset
|
578 <metadata name="column_names" value="Status,featureCounts_input1.bam"/> |
|
d0beb2753aba
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 4c095ddb0f2c369d2bf3fc5ca386f6981a2fa0eb
iuc
parents:
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diff
changeset
|
579 </output> |
|
d0beb2753aba
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 4c095ddb0f2c369d2bf3fc5ca386f6981a2fa0eb
iuc
parents:
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diff
changeset
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580 </test> |
|
18
34b78acbad1d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit b467fdf3fabeed440dcb596108fc792d44e7d8d9
iuc
parents:
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diff
changeset
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581 <!-- Ensure BAM output works --> |
|
34
12b3317716d3
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit fc2a03ae0ef4d1d0d5f2f05a6ecc0fc4812931e1
iuc
parents:
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582 <test expect_num_outputs="3"> |
|
32
456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents:
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diff
changeset
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583 <param name="alignment" value="subset.sorted.bam" ftype="bam"/> |
|
456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents:
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diff
changeset
|
584 <param name="anno_select" value="history"/> |
|
456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents:
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diff
changeset
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585 <param name="reference_gene_sets" value="small.gtf" ftype="gtf"/> |
|
456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents:
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diff
changeset
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586 <section name="extended_parameters"> |
|
456cc2927264
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents:
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diff
changeset
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587 <param name="R" value="true"/> |
|
18
34b78acbad1d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit b467fdf3fabeed440dcb596108fc792d44e7d8d9
iuc
parents:
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diff
changeset
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588 </section> |
|
21
d4d69c4d0780
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a956e32b176d645db54db3c86586cc0bfa2fbd3c
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diff
changeset
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589 <output name="output_bam" value="subset.sorted.featurecounts.bam" compare="sim_size"/> |
|
34
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit fc2a03ae0ef4d1d0d5f2f05a6ecc0fc4812931e1
iuc
parents:
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diff
changeset
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590 <output name="output_short" file="subset.sorted.featurecounts_short.tab"> |
|
12b3317716d3
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit fc2a03ae0ef4d1d0d5f2f05a6ecc0fc4812931e1
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parents:
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diff
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|
591 <metadata name="column_names" value="Geneid,subset.sorted.bam"/> |
|
12b3317716d3
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit fc2a03ae0ef4d1d0d5f2f05a6ecc0fc4812931e1
iuc
parents:
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diff
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|
592 </output> |
|
12b3317716d3
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit fc2a03ae0ef4d1d0d5f2f05a6ecc0fc4812931e1
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593 <output name="output_summary" file="subset.sorted.featurecounts_summary.tab"/> |
|
18
34b78acbad1d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit b467fdf3fabeed440dcb596108fc792d44e7d8d9
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diff
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|
594 </test> |
|
0
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
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changeset
|
595 </tests> |
|
8
bff059ad4d13
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2228563ac1c2bb236c8ac83d08d2b86e21a39e41
iuc
parents:
7
diff
changeset
|
596 |
|
0
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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changeset
|
597 <help><![CDATA[ |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
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changeset
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598 featureCounts |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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changeset
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599 ############# |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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changeset
|
600 |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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parents:
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changeset
|
601 Overview |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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changeset
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602 -------- |
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10
7d1e37c6ef85
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
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603 FeatureCounts is a light-weight read counting program written entirely in the C programming language. It can be used to count both gDNA-seq and RNA-seq reads for genomic features in in SAM/BAM files. FeatureCounts is part of the Subread_ package. |
|
0
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
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changeset
|
604 |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
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changeset
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605 Input formats |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
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changeset
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606 ------------- |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
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changeset
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607 Alignments should be provided in either: |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
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changeset
|
608 |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
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changeset
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609 - SAM format, http://samtools.sourceforge.net/samtools.shtml#5 |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
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changeset
|
610 - BAM format |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
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changeset
|
611 |
|
10
7d1e37c6ef85
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
iuc
parents:
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diff
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612 Annotations for gene regions should be provided in the GFF/GTF format: |
|
0
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
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changeset
|
613 |
|
8e70555968a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
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614 - http://genome.ucsc.edu/FAQ/FAQformat.html#format3 |
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615 - http://www.ensembl.org/info/website/upload/gff.html |
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616 |
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617 Alternatively, the featureCounts built-in annotations for genomes hg38, hg19, mm10 and mm9 can be used through selecting the built-in option above. These annotation files are in simplified annotation format (SAF) as shown below. The GeneID column contains Entrez gene identifiers and each entry (row) is taken as a feature (e.g. an exon). |
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618 |
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619 Example - **Built-in annotation format**: |
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620 |
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621 ====== ==== ======= ======= ====== |
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622 GeneID Chr Start End Strand |
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623 ====== ==== ======= ======= ====== |
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624 497097 chr1 3204563 3207049 - |
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625 497098 chr1 3411783 3411982 - |
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626 497099 chr1 3660633 3661579 - |
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627 ====== ==== ======= ======= ====== |
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628 |
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629 These annotation files can be found in the _`Subread package`_. You can see the version of Subread used by this wrapper in the tool form above under `Options > Requirements`. To create the files, the annotations were downloaded from NCBI RefSeq database and then adapted by merging overlapping exons from the same gene to form a set of disjoint exons for each gene. Genes with the same Entrez gene identifiers were also merged into one gene. See the `Subread User's Guide`_ for more information. Gene names can be obtained for these Entrez identifiers with the Galaxy **annotateMyIDs** tool. |
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630 |
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631 Output format |
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632 ------------- |
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633 FeatureCounts produces a table containing counted reads, per gene, per row. Optionally the last column can be set to be the effective gene-length. These tables are compatible with the DESeq2, edgeR and limma-voom Galaxy wrappers by IUC. |
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634 |
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635 .. _Subread: http://subread.sourceforge.net/ |
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636 .. _`Subread User's Guide`: https://bioconductor.org/packages/release/bioc/vignettes/Rsubread/inst/doc/SubreadUsersGuide.pdf |
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637 ]]></help> |
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638 <citations> |
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639 <citation type="doi">10.1093/bioinformatics/btt656</citation> |
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640 </citations> |
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641 </tool> |
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