annotate featurecounts.xml @ 37:8620533d294b draft default tip

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit c43e4b51d4b0a46eeba363fa4061d4af03a677a4
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date Wed, 07 May 2025 09:59:13 +0000
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1 <tool id="featurecounts" name="featureCounts" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="23.2">
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2 <description>Measure gene expression in RNA-Seq experiments from SAM or BAM files</description>
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3 <macros>
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4 <token name="@TOOL_VERSION@">2.1.1</token>
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5 <token name="@VERSION_SUFFIX@">0</token>
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6 <macro name="conditional_gff_opions">
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7 <param name="gff_feature_type" type="text" value="exon" argument="-t" label="GFF feature type filter" help="Specify the feature type. Only rows which have the matched matched feature type in the provided GTF annotation file will be included for read counting. `exon' by default."/>
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8 <param name="gff_feature_attribute" type="text" value="gene_id" argument="-g" label="GFF gene identifier" help="Specify the attribute type used to group features (eg. exons) into meta-features (eg. genes), when GTF annotation is provided. `gene_id' by default. This attribute type is usually the gene identifier. This argument is useful for the meta-feature level summarization. Ex: if the 9th column is 'gene_id &quot;ENSG00000223972&quot;; gene_name &quot;DDX11L1&quot; gene_source &quot;havana&quot;' (GTF) or 'gene_id=ENSG00000223972; gene_name=DDX11L1; gene_source=havana' (GFF), the available attributes for this feature are 'gene_id', 'gene_name' and 'gene_source'."/>
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9 <param name="summarization_level" type="boolean" truevalue=" -f" falsevalue="" argument="-f" label="On feature level" help="If specified, read summarization will be performed at the feature level. By default (-f is not specified), the read summarization is performed at the meta-feature level."/>
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10 </macro>
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11 </macros>
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12 <xrefs>
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13 <xref type="bio.tools">featurecounts</xref>
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14 </xrefs>
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15 <requirements>
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16 <requirement type="package" version="@TOOL_VERSION@">subread</requirement>
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17 <requirement type="package" version="1.21">samtools</requirement>
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18 </requirements>
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20 <version_command>featureCounts -v 2&gt;&amp;1 | grep .</version_command>
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21 <command detect_errors="exit_code"><![CDATA[
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22 ## Export fc path for its built-in annotation
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23 export FC_PATH=\$(command -v featureCounts | sed 's@/bin/featureCounts$@@') &&
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24
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25 ## Check whether all alignments are from the same type (bam || sam)
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26 featureCounts
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28 #if $anno.anno_select=="history":
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29 -a '$anno.reference_gene_sets'
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30 -F "GTF"
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31 #elif $anno.anno_select=="cached":
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32 -a '$anno.reference_gene_sets_cached.fields.path'
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33 -F "GTF"
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34 #elif $anno.anno_select=="builtin":
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35 -a \${FC_PATH}/annotation/${anno.bgenome}_RefSeq_exon.txt
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36 -F "SAF"
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37 #end if
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38
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39 -o "output"
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40 -T \${GALAXY_SLOTS:-2}
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41
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42 -s $strand_specificity
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44 -Q $read_filtering_parameters.mapping_quality
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45 $read_filtering_parameters.splitonly
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46 $read_filtering_parameters.primary
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47 $read_filtering_parameters.ignore_dup
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48
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49 #if $anno.anno_select!="builtin":
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50 -t '$anno.gff_feature_type'
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51 -g '$anno.gff_feature_attribute'
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52 $anno.summarization_level
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53 #end if
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55 $extended_parameters.multifeatures.multifeat
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56 #if $extended_parameters.multifeatures.multifeat != "":
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57 $extended_parameters.multifeatures.fraction
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58 #end if
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59
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60 $extended_parameters.exon_exon_junction_read_counting_enabled.count_exon_exon_junction_reads
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61 #if str($extended_parameters.exon_exon_junction_read_counting_enabled.count_exon_exon_junction_reads) == "-J":
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62 #if $extended_parameters.exon_exon_junction_read_counting_enabled.genome:
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63 -G '$extended_parameters.exon_exon_junction_read_counting_enabled.genome'
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64 #end if
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65 #end if
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67 $extended_parameters.long_reads
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68
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69 $extended_parameters.by_read_group
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70
0
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71 $extended_parameters.largest_overlap
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72 --minOverlap $extended_parameters.min_overlap
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73 --fracOverlap $extended_parameters.frac_overlap
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74 --fracOverlapFeature $extended_parameters.frac_overlap_feature
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75 $extended_parameters.read_reduction
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76 #if $extended_parameters.R:
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77 $extended_parameters.R
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78 #end if
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79 #if str($extended_parameters.read_extension_5p) != "0":
0
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80 --readExtension5 $extended_parameters.read_extension_5p
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81 #end if
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82
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83 #if str($extended_parameters.read_extension_3p) != "0":
0
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84 --readExtension3 $extended_parameters.read_extension_3p
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85 #end if
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86
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87 #if str($pe_parameters.paired_end_status) != "single_end":
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88 -p
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89 $pe_parameters.only_both_ends
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90 $pe_parameters.exclude_chimerics
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91 #if str($pe_parameters.paired_end_status) == "PE_fragments":
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92 --countReadPairs
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93 #if $pe_parameters.check_distance_enabled.checkFragLength == "true":
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94 --checkFragLength
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95 --minFragLength $pe_parameters.check_distance_enabled.minimum_fragment_length
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96 --maxFragLength $pe_parameters.check_distance_enabled.maximum_fragment_length
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97 #end if
0
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98 #end if
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99 #end if
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100
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101 '${alignment}'
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102
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103 ## Remove comment and add sample name to header
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104 && grep -v "^#" "output"
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105 | sed -e 's|${alignment}|${alignment.element_identifier}|g'
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106 > body.txt
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107 ## Set the right columns for the tabular formats
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108 #if $format.value == "tabdel_medium":
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109 && cut -f 1,7 body.txt > expression_matrix.txt
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110
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111 ## Paste doesn't allow a non ordered list of columns: -f 1,7,8,6 will only return columns 1,7 and 8
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112 ## Thus the gene length column (last column) has to be added separately
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113 && cut -f 6 body.txt > gene_lengths.txt
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114 && paste expression_matrix.txt gene_lengths.txt > expression_matrix.txt.bak
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115 && mv -f expression_matrix.txt.bak '${output_medium}'
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116 #elif $format.value == "tabdel_short":
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117 && cut -f 1,7 body.txt > '${output_short}'
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118 #else:
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119 && cp body.txt '${output_full}'
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120 #end if
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121
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122 #if str($include_feature_length_file) == "true":
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123 && cut -f 1,6 body.txt > '${output_feature_lengths}'
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124 #end if
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125
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126 #if str($extended_parameters.exon_exon_junction_read_counting_enabled.count_exon_exon_junction_reads) == "-J":
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127 && sed -e 's|${alignment}|${alignment.element_identifier}|g' 'output.jcounts' > '${output_jcounts}'
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128 #end if
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129
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130 #if $extended_parameters.R:
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131 && samtools sort --no-PG -o '$output_bam' -@ \${GALAXY_SLOTS:-2} -T "\${TMPDIR:-.}" *.featureCounts.bam
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132 #end if
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133 && sed -e 's|${alignment}|${alignment.element_identifier}|g' 'output.summary' > '${output_summary}'
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134 ]]></command>
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135 <inputs>
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136 <param name="alignment"
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137 type="data"
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138 multiple="false"
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139 format="unsorted.bam,bam,sam"
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140 label="Alignment file"
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141 help="The input alignment file(s) where the gene expression has to be counted. The file can have a SAM or BAM format; but ALL files must be in the same format. Unless you are using a Gene annotation file from the History, these files must have the database/genome attribute already specified e.g. hg38, not the default: ?">
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142 </param>
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143
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144 <param name="strand_specificity"
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145 type="select"
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146 label="Specify strand information"
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147 argument="-s"
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148 help="Indicate if the data is stranded and if strand-specific read counting should be performed. Strand setting must be the same as the strand settings used to produce the mapped BAM input(s)">
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149 <option value="0" selected="true">Unstranded</option>
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150 <option value="1">Stranded (Forward)</option>
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151 <option value="2">Stranded (Reverse)</option>
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152 </param>
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153
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154 <conditional name="anno">
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155 <param name="anno_select" type="select" label="Gene annotation file">
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156 <option value="builtin">featureCounts built-in</option>
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157 <option value="cached" selected="True">locally cached</option>
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158 <option value="history">A GFF/GTF file in your history</option>
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159 </param>
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160 <when value="builtin">
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161 <param name="bgenome" type="select" label="Select built-in genome" help="Built-in gene annotations for genomes hg38, hg19, mm10 and mm9 are included in featureCounts">
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162 <options from_data_table="featurecounts_anno">
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163 <filter type="data_meta" key="dbkey" ref="alignment" column="dbkey"/>
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164 <filter type="sort_by" column="1"/>
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165 </options>
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166 <validator type="no_options" message="An built-in annotation file is not available for the genome build associated with the selected input file"/>
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167 </param>
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168 </when>
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169 <when value="cached">
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170 <param name="reference_gene_sets_cached" type="select" label="Using locally cached annotation" help="If the annotation file you require is not listed here, please contact the Galaxy administrator.">
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171 <options from_data_table="gene_sets">
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172 <filter type="data_meta" key="dbkey" ref="alignment" column="dbkey"/>
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173 <filter type="sort_by" column="2"/>
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174 </options>
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175 <validator type="no_options" message="A cached annotation file is not available for the genome build associated with the selected input file"/>
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176 </param>
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177 <expand macro="conditional_gff_opions"/>
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178 </when>
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179 <when value="history">
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180 <param name="reference_gene_sets"
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181 format="gff,gtf,gff3"
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182 type="data"
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183 label="Gene annotation file"
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184 help="The program assumes that the provided annotation file is in GFF/GTF format. Make sure that the gene annotation file corresponds to the same reference genome as used for the alignment.">
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185 </param>
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186 <expand macro="conditional_gff_opions"/>
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187 </when>
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188 </conditional>
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189
0
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190 <param name="format"
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191 type="select"
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192 label="Output format"
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193 help="The output format will be tabular, select the preferred columns here">
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194 <option value="tabdel_short">Gene-ID "\t" read-count (MultiQC/DESeq2/edgeR/limma-voom compatible)</option>
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195 <option value="tabdel_medium">Gene-ID "\t" read-count "\t" gene-length</option>
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196 <option value="tabdel_full">featureCounts 1.4.0+ default (includes regions provided by the GTF file)</option>
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197 </param>
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198
0
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199 <param name="include_feature_length_file"
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200 type="boolean"
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201 truevalue="true"
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202 falsevalue="false"
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203 checked="false"
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204 label="Create gene-length file"
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205 help="Creates a tabular file that contains the effective (nucleotides used for counting reads) length of the feature; might be useful for estimating FPKM/RPKM"/>
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206
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207 <conditional name="pe_parameters">
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208 <param name="paired_end_status" type="select" label="Does the input have read pairs?" help="Were the bam files generated by aligning the output of a paired-end sequencing experiment? If yes, the tool can consider 2 reads = 1 read pair as 1 entity to count. Alternatively, you can opt to consider treating the read pairs as 2 individual reads to count seperately.">
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209 <option value="single_end" selected="True">No, single-end.</option>
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210 <option value="PE_individual">Yes, paired-end but still count them as if individual reads.</option>
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211 <option value="PE_fragments">Yes, paired-end and count them as 1 single fragment.</option>
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212 </param>
0
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213
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214 <when value="single_end"/>
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215 <when value="PE_individual">
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216 <param name="only_both_ends"
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217 type="boolean"
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218 truevalue=" -B"
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219 falsevalue=""
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220 argument="-B"
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221 label="Only allow fragments with both reads aligned"
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222 help="If specified, only fragments that have both ends successfully aligned will be considered for summarization. This option is only applicable for paired-end reads."/>
0
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223
32
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224 <param name="exclude_chimerics"
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225 type="boolean"
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226 truevalue=" -C"
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227 falsevalue=""
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228 argument="-C"
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229 checked="true"
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230 label="Exclude chimeric fragments"
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231 help="If specified, the chimeric fragments (those fragments that have their two ends aligned to different chromosomes) will NOT be included for summarization. This option is only applicable for paired-end read data."/>
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232 </when>
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233 <when value="PE_fragments">
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234 <conditional name="check_distance_enabled">
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235 <param argument="--checkFragLength" type="select" label="Check paired-end distance" help="If specified, paired-end distance will be checked when assigning fragments to meta-features or features. This option is only applicable when -p (Count fragments instead of reads) is specified. The distance thresholds can be specified using -d and -D (minimum and maximum fragment/template length) options.">
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236 <option value="true">Check the distance between paired reads</option>
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237 <option value="false" selected="True">Do not check</option>
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238 </param>
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239 <when value="true">
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240 <param name="minimum_fragment_length"
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241 type="integer"
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242 value="50"
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243 min="0"
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244 argument="-d"
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245 label="Minimum fragment/template length."/>
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246 <param name="maximum_fragment_length"
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247 type="integer"
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248 value="600"
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249 min="1"
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250 argument="-D"
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251 label="Maximum fragment/template length."/>
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252 </when>
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253 <when value="false"/>
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254 </conditional>
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255
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256 <param name="only_both_ends"
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257 type="boolean"
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258 truevalue=" -B"
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259 falsevalue=""
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260 argument="-B"
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261 label="Only allow fragments with both reads aligned"
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262 help="If specified, only fragments that have both ends successfully aligned will be considered for summarization. This option is only applicable for paired-end reads."/>
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263
32
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264 <param name="exclude_chimerics"
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265 type="boolean"
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266 truevalue=" -C"
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267 falsevalue=""
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268 argument="-C"
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269 checked="true"
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270 label="Exclude chimeric fragments"
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271 help="If specified, the chimeric fragments (those fragments that have their two ends aligned to different chromosomes) will NOT be included for summarization. This option is only applicable for paired-end read data."/>
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272 </when>
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273 </conditional>
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274
31
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275 <section name="read_filtering_parameters" title="Read filtering options">
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276 <param name="mapping_quality"
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277 type="integer"
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278 value="0"
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279 min="0"
31
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280 argument="-Q"
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281 label="Minimum mapping quality per read"
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282 help="The minimum mapping quality score a read must satisfy in order to be counted. For paired-end reads, at least one end should satisfy this criteria. 0 by default."/>
31
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283 <param name="splitonly" type="select" display="radio" label="Filter split alignments" help="Split alignments are alignments with CIGAR string containing 'N', e.g. exon spanning reads in RNASeq.">
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284 <option value="">No filtering: count split and non-split alignments</option>
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285 <option value="--splitOnly">Count only split alignments (--splitOnly)</option>
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286 <option value="--nonSplitOnly">Count only non-split alignments (--nonSplitOnly)</option>
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287 </param>
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288 <param type="boolean"
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289 truevalue=" --primary"
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290 falsevalue=""
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291 argument="--primary"
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292 label="Only count primary alignments"
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293 help="If specified, only primary alignments will be counted. Primary and secondary alignments are identified using bit 0x100 in theFlag field of SAM/BAM files. All primary alignments in a dataset will be counted regardless of whether they are from multi-mapping reads or not ('-M' is ignored)."/>
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294 <param name="ignore_dup"
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295 type="boolean"
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296 truevalue=" --ignoreDup"
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297 falsevalue=""
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298 argument="--ignoreDup"
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299 label="Ignore reads marked as duplicate"
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300 help="If specified, reads that were marked as duplicates will be ignored. Bit Ox400 in the FLAG field of a SAM/BAM file is used for identifying duplicate reads. In paired end data, the entire read pair will be ignored if at least one end is found to be a duplicate read."/>
31
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301 </section>
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302
0
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303 <section name="extended_parameters" title="Advanced options">
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304 <conditional name = "multifeatures">
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305 <param name="multifeat" type="select" label="Allow reads to map to multiple features" help="Setting -O, -M and --fraction">
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306 <option value="" selected="true">Disabled: reads that align to multiple features or overlapping features are excluded</option>
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307 <option value="-M">Enabled: multi-mapping reads are included (-M)</option>
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308 <option value="-O">Enabled: multi-overlapping features are included (-O)</option>
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309 <option value="-O -M">Enabled: both multi-mapping and multi-overlapping features are included (-M -O)</option>
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310 </param>
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311 <when value=""/>
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312 <when value="-M">
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313 <param type="boolean"
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314 truevalue="--fraction"
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315 falsevalue=""
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316 argument="--fraction"
31
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317 label="Assign fractions to multi-mapping reads"
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318 help="If specified, a fractional count 1/x will be generated for each multi-mapping read, where x is the number of alignments (indicated by 'NH' tag) reported for the read."/>
0
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319 </when>
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320 <when value="-O">
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321 <param type="boolean"
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322 truevalue="--fraction"
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323 falsevalue=""
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324 argument="--fraction"
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325 label="Assign fractions to multi-overlapping features"
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326 help="If specified, a fractional count 1/y will be generated for each multi-overlapping feature, where y is the number of features overlapping with the read."/>
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327 </when>
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328 <when value="-O -M">
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329 <param type="boolean"
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330 truevalue="--fraction"
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331 falsevalue=""
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332 argument="--fraction"
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333 label="Assign fractions to both multi-mapping reads and multi-overlapping features"
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334 help="If specified, a fractional count 1/(x*y) will be generated, where x is the number of alignments (indicated by 'NH' tag) and y the number of overlapping features."/>
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335 </when>
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336 </conditional>
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337
6
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338 <conditional name="exon_exon_junction_read_counting_enabled">
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339 <param name="count_exon_exon_junction_reads" type="select" argument="-J" label="Exon-exon junctions" help="Junctions are identified from those exon-spanning reads (containing ‘N’ in CIGAR string) in input data. The output result includes names of primary and secondary genes that overlap at least one of the two splice sites of a junction.">
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340 <option value="-J">Count reads supporting each exon-exon junction.</option>
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341 <option value="" selected="True">Do not count</option>
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342 </param>
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343 <when value="-J">
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344 <param name="genome" argument="-G" type="data" format="fasta" optional="true"
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345 label="Reference sequence file"
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diff changeset
346 help="The FASTA-format file that contains the reference sequences used in read mapping can be used to improve read counting for junctions"/>
6
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parents: 4
diff changeset
347 </when>
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parents: 31
diff changeset
348 <when value=""/>
6
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parents: 4
diff changeset
349 </conditional>
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parents: 4
diff changeset
350
e70afb590e50 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit ffb40a055701dca74e29e651dd820a6bda3d844b
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parents: 4
diff changeset
351 <param name="long_reads" argument="-L" type="boolean" truevalue="-L" falsevalue=""
e70afb590e50 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit ffb40a055701dca74e29e651dd820a6bda3d844b
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parents: 4
diff changeset
352 label="Long reads"
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parents: 31
diff changeset
353 help="If specified, long reads such as Nanopore and PacBio reads will be counted. Long read counting can only run in one thread and only reads (not read-pairs) can be counted."/>
6
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parents: 4
diff changeset
354
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parents: 9
diff changeset
355 <param name="by_read_group" argument="--byReadGroup" type="boolean" truevalue="--byReadGroup" falsevalue=""
8
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parents: 7
diff changeset
356 label="Count reads by read group"
32
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parents: 31
diff changeset
357 help="If specified, reads are counted for each read group separately. The 'RG' tag must be present in the input BAM/SAM alignment files."/>
8
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parents: 7
diff changeset
358
0
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
359 <param name="largest_overlap"
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
360 type="boolean"
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
361 truevalue=" --largestOverlap"
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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parents:
diff changeset
362 falsevalue=""
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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diff changeset
363 argument="--largestOverlap"
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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diff changeset
364 label="Largest overlap"
32
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iuc
parents: 31
diff changeset
365 help="If specified, reads (or fragments) will be assigned to the target that has the largest number of overlapping bases"/>
0
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
366
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
367 <param name="min_overlap"
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
368 type="integer"
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
369 value="1"
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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diff changeset
370 argument="--minOverlap"
8
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parents: 7
diff changeset
371 label="Minimum bases of overlap"
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parents: 31
diff changeset
372 help="Specify the minimum required number of overlapping bases between a read (or a fragment) and a feature. 1 by default. If a negative value is provided, the read will be extended from both ends."/>
0
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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diff changeset
373
10
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parents: 9
diff changeset
374 <param name="frac_overlap"
8
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iuc
parents: 7
diff changeset
375 type="integer"
bff059ad4d13 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2228563ac1c2bb236c8ac83d08d2b86e21a39e41
iuc
parents: 7
diff changeset
376 value="0"
bff059ad4d13 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2228563ac1c2bb236c8ac83d08d2b86e21a39e41
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parents: 7
diff changeset
377 min="0"
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parents: 7
diff changeset
378 max="1"
bff059ad4d13 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2228563ac1c2bb236c8ac83d08d2b86e21a39e41
iuc
parents: 7
diff changeset
379 argument="--fracOverlap"
bff059ad4d13 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2228563ac1c2bb236c8ac83d08d2b86e21a39e41
iuc
parents: 7
diff changeset
380 label="Minimum fraction (of read) overlapping a feature"
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parents: 31
diff changeset
381 help="Specify the minimum required fraction of overlapping bases between a read (or a fragment) and a feature. Value should be within range [0,1]. 0 by default. Number of overlapping bases is counted from both reads if paired end. Both this option and '--minOverlap' need to be satisfied for read assignment."/>
8
bff059ad4d13 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2228563ac1c2bb236c8ac83d08d2b86e21a39e41
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parents: 7
diff changeset
382
10
7d1e37c6ef85 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
iuc
parents: 9
diff changeset
383 <param name="frac_overlap_feature"
8
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iuc
parents: 7
diff changeset
384 type="integer"
bff059ad4d13 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2228563ac1c2bb236c8ac83d08d2b86e21a39e41
iuc
parents: 7
diff changeset
385 value="0"
bff059ad4d13 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2228563ac1c2bb236c8ac83d08d2b86e21a39e41
iuc
parents: 7
diff changeset
386 min="0"
bff059ad4d13 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2228563ac1c2bb236c8ac83d08d2b86e21a39e41
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parents: 7
diff changeset
387 max="1"
bff059ad4d13 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2228563ac1c2bb236c8ac83d08d2b86e21a39e41
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parents: 7
diff changeset
388 argument="--fracOverlapFeature"
bff059ad4d13 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2228563ac1c2bb236c8ac83d08d2b86e21a39e41
iuc
parents: 7
diff changeset
389 label="Minimum fraction (of feature) overlapping a read"
32
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parents: 31
diff changeset
390 help="Specify the minimum required fraction of bases included in a feature overlapping bases between a read (or a read-pair). Value should be within range [0,1]. 0 by default."/>
8
bff059ad4d13 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2228563ac1c2bb236c8ac83d08d2b86e21a39e41
iuc
parents: 7
diff changeset
391
0
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
392 <param name="read_extension_5p"
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
393 type="integer"
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
394 value="0"
32
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iuc
parents: 31
diff changeset
395 min="0"
0
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
396 argument="--readExtension5"
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
397 label="Read 5' extension"
32
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parents: 31
diff changeset
398 help="Reads are extended upstream by ... bases from their 5' end"/>
0
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
399
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
400 <param name="read_extension_3p"
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
401 type="integer"
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
402 value="0"
32
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iuc
parents: 31
diff changeset
403 min="0"
0
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
404 argument="--readExtension3"
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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parents:
diff changeset
405 label="Read 3' extension"
32
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iuc
parents: 31
diff changeset
406 help="Reads are extended upstream by ... bases from their 3' end"/>
0
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
407
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
408 <param name="read_reduction"
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
409 type="select"
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
410 label="Reduce read to single position"
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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parents:
diff changeset
411 argument="--read2pos"
3
711c80e57384 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 1c0d28b6cefe154e8cf037c9f36200e8f52a838f
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parents: 2
diff changeset
412 help="The read is reduced to its 5' most base or 3'most base. Read summarization is then performed based on the single base the the read is reduced to.">
0
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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diff changeset
413 <option value="" selected="true">Leave the read as it is</option>
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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diff changeset
414 <option value="--read2pos 5">Reduce it to the 5' end</option>
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
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diff changeset
415 <option value="--read2pos 3">Reduce it to the 3' end</option>
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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diff changeset
416 </param>
8
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parents: 7
diff changeset
417
17
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parents: 15
diff changeset
418 <param type="boolean"
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parents: 15
diff changeset
419 truevalue="-R BAM"
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parents: 15
diff changeset
420 falsevalue=""
5c20eb47f759 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 6c859db1a131f3f17aecdf44af80ad93b0e9c018
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parents: 15
diff changeset
421 argument="-R"
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parents: 15
diff changeset
422 label="Annotates the alignment file with 'XS:Z:'-tags to described per read or read-pair the corresponding assigned feature(s)."
32
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parents: 31
diff changeset
423 help=""/>
17
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iuc
parents: 15
diff changeset
424
0
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
425 </section>
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
426 </inputs>
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
427 <outputs>
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
428 <data format="tabular"
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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parents:
diff changeset
429 name="output_medium"
20
b4f3420c2a70 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit c8c08f052bdffcbfd14eebd760af923486bcf8c5
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parents: 19
diff changeset
430 label="${tool.name} on ${on_string}: Counts (with length)">
0
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
431 <filter>format == "tabdel_medium"</filter>
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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diff changeset
432 <actions>
32
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parents: 31
diff changeset
433 <action name="column_names" type="metadata" default="Geneid,${alignment.element_identifier},Length"/>
0
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
434 </actions>
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
435 </data>
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
436
17
5c20eb47f759 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 6c859db1a131f3f17aecdf44af80ad93b0e9c018
iuc
parents: 15
diff changeset
437 <data format="bam"
5c20eb47f759 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 6c859db1a131f3f17aecdf44af80ad93b0e9c018
iuc
parents: 15
diff changeset
438 name="output_bam"
5c20eb47f759 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 6c859db1a131f3f17aecdf44af80ad93b0e9c018
iuc
parents: 15
diff changeset
439 label="${tool.name} on ${on_string}: Alignment file">
5c20eb47f759 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 6c859db1a131f3f17aecdf44af80ad93b0e9c018
iuc
parents: 15
diff changeset
440 <filter>extended_parameters['R']</filter>
5c20eb47f759 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 6c859db1a131f3f17aecdf44af80ad93b0e9c018
iuc
parents: 15
diff changeset
441 </data>
5c20eb47f759 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 6c859db1a131f3f17aecdf44af80ad93b0e9c018
iuc
parents: 15
diff changeset
442
0
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
443 <data format="tabular"
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
444 name="output_short"
20
b4f3420c2a70 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit c8c08f052bdffcbfd14eebd760af923486bcf8c5
iuc
parents: 19
diff changeset
445 label="${tool.name} on ${on_string}: Counts">
13
c7085a3028ef planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2bd06c2b43c295fb4cf172c4f156fed5475855a4
iuc
parents: 12
diff changeset
446 <filter>format == "tabdel_short"</filter>
0
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
447 <actions>
32
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iuc
parents: 31
diff changeset
448 <action name="column_names" type="metadata" default="Geneid,${alignment.element_identifier}"/>
0
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
449 </actions>
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
450 </data>
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
451
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
452 <data format="tabular"
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
453 name="output_full"
20
b4f3420c2a70 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit c8c08f052bdffcbfd14eebd760af923486bcf8c5
iuc
parents: 19
diff changeset
454 label="${tool.name} on ${on_string}: Counts (with location)">
0
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
455 <filter>format == "tabdel_full"</filter>
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
456 <actions>
32
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iuc
parents: 31
diff changeset
457 <action name="column_names" type="metadata" default="Geneid,Chr,Start,End,Strand,Length,${alignment.element_identifier}"/>
0
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
458 </actions>
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
459 </data>
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
460
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
461 <data format="tabular"
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
462 name="output_summary"
20
b4f3420c2a70 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit c8c08f052bdffcbfd14eebd760af923486bcf8c5
iuc
parents: 19
diff changeset
463 label="${tool.name} on ${on_string}: Summary">
0
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
464 <actions>
32
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iuc
parents: 31
diff changeset
465 <action name="column_names" type="metadata" default="Status,${alignment.element_identifier}"/>
0
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
466 </actions>
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
467 </data>
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
468
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
469 <data format="tabular"
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
470 name="output_feature_lengths"
20
b4f3420c2a70 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit c8c08f052bdffcbfd14eebd760af923486bcf8c5
iuc
parents: 19
diff changeset
471 label="${tool.name} on ${on_string}: Feature lengths">
6
e70afb590e50 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit ffb40a055701dca74e29e651dd820a6bda3d844b
iuc
parents: 4
diff changeset
472 <filter>include_feature_length_file</filter>
0
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
473 <actions>
32
456cc2927264 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents: 31
diff changeset
474 <action name="column_names" type="metadata" default="Feature,Length"/>
0
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
475 </actions>
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
476 </data>
6
e70afb590e50 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit ffb40a055701dca74e29e651dd820a6bda3d844b
iuc
parents: 4
diff changeset
477
e70afb590e50 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit ffb40a055701dca74e29e651dd820a6bda3d844b
iuc
parents: 4
diff changeset
478 <data name="output_jcounts" format="tabular"
20
b4f3420c2a70 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit c8c08f052bdffcbfd14eebd760af923486bcf8c5
iuc
parents: 19
diff changeset
479 label="${tool.name} on ${on_string}: Junction counts">
7
889d2cd8a809 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a705dfd329f2e917d549215715385f5ef5001d17
iuc
parents: 6
diff changeset
480 <filter>extended_parameters['exon_exon_junction_read_counting_enabled']['count_exon_exon_junction_reads']</filter>
6
e70afb590e50 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit ffb40a055701dca74e29e651dd820a6bda3d844b
iuc
parents: 4
diff changeset
481 <actions>
10
7d1e37c6ef85 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
iuc
parents: 9
diff changeset
482 <action name="column_names" type="metadata"
32
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iuc
parents: 31
diff changeset
483 default="PrimaryGene,SecondaryGene,Site1_chr,Site1_location,Site1_strand,Site2_chr,Site2_location,Site2_strand,${alignment.element_identifier}"/>
6
e70afb590e50 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit ffb40a055701dca74e29e651dd820a6bda3d844b
iuc
parents: 4
diff changeset
484 </actions>
e70afb590e50 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit ffb40a055701dca74e29e651dd820a6bda3d844b
iuc
parents: 4
diff changeset
485 </data>
0
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
486 </outputs>
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
487 <tests>
7
889d2cd8a809 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a705dfd329f2e917d549215715385f5ef5001d17
iuc
parents: 6
diff changeset
488 <test expect_num_outputs="3">
34
12b3317716d3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit fc2a03ae0ef4d1d0d5f2f05a6ecc0fc4812931e1
iuc
parents: 33
diff changeset
489 <param name="alignment" value="featureCounts_input1.bam" ftype="unsorted.bam" dbkey="hg38"/>
11
d0beb2753aba planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 4c095ddb0f2c369d2bf3fc5ca386f6981a2fa0eb
iuc
parents: 10
diff changeset
490 <param name="anno_select" value="history"/>
32
456cc2927264 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents: 31
diff changeset
491 <param name="reference_gene_sets" value="featureCounts_guide.gff" ftype="gff" dbkey="hg38"/>
456cc2927264 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents: 31
diff changeset
492 <param name="format" value="tabdel_medium"/>
0
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
493 <param name="include_feature_length_file" value="true"/>
4
2451333b004b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 57dfb61ba50ecfbe8f1c624fdbe2d0a3a8deaa04
iuc
parents: 3
diff changeset
494 <output name="output_medium" file="output_1_medium.tab">
2451333b004b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 57dfb61ba50ecfbe8f1c624fdbe2d0a3a8deaa04
iuc
parents: 3
diff changeset
495 <metadata name="column_names" value="Geneid,featureCounts_input1.bam,Length"/>
2451333b004b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 57dfb61ba50ecfbe8f1c624fdbe2d0a3a8deaa04
iuc
parents: 3
diff changeset
496 </output>
2451333b004b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 57dfb61ba50ecfbe8f1c624fdbe2d0a3a8deaa04
iuc
parents: 3
diff changeset
497 <output name="output_summary" file="output_1_summary.tab">
2451333b004b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 57dfb61ba50ecfbe8f1c624fdbe2d0a3a8deaa04
iuc
parents: 3
diff changeset
498 <metadata name="column_names" value="Status,featureCounts_input1.bam"/>
2451333b004b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 57dfb61ba50ecfbe8f1c624fdbe2d0a3a8deaa04
iuc
parents: 3
diff changeset
499 </output>
0
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
500 </test>
7
889d2cd8a809 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a705dfd329f2e917d549215715385f5ef5001d17
iuc
parents: 6
diff changeset
501 <test expect_num_outputs="3">
34
12b3317716d3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit fc2a03ae0ef4d1d0d5f2f05a6ecc0fc4812931e1
iuc
parents: 33
diff changeset
502 <param name="alignment" value="featureCounts_input1.bam" ftype="unsorted.bam" dbkey="hg38"/>
11
d0beb2753aba planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 4c095ddb0f2c369d2bf3fc5ca386f6981a2fa0eb
iuc
parents: 10
diff changeset
503 <param name="anno_select" value="history"/>
32
456cc2927264 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents: 31
diff changeset
504 <param name="reference_gene_sets" value="featureCounts_guide.gff" ftype="gff" dbkey="hg38"/>
456cc2927264 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents: 31
diff changeset
505 <param name="format" value="tabdel_full"/>
0
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
506 <param name="include_feature_length_file" value="true"/>
4
2451333b004b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 57dfb61ba50ecfbe8f1c624fdbe2d0a3a8deaa04
iuc
parents: 3
diff changeset
507 <output name="output_full" file="output_1_full.tab">
2451333b004b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 57dfb61ba50ecfbe8f1c624fdbe2d0a3a8deaa04
iuc
parents: 3
diff changeset
508 <metadata name="column_names" value="Geneid,Chr,Start,End,Strand,Length,featureCounts_input1.bam"/>
2451333b004b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 57dfb61ba50ecfbe8f1c624fdbe2d0a3a8deaa04
iuc
parents: 3
diff changeset
509 </output>
2451333b004b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 57dfb61ba50ecfbe8f1c624fdbe2d0a3a8deaa04
iuc
parents: 3
diff changeset
510 <output name="output_summary" file="output_1_summary.tab">
2451333b004b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 57dfb61ba50ecfbe8f1c624fdbe2d0a3a8deaa04
iuc
parents: 3
diff changeset
511 <metadata name="column_names" value="Status,featureCounts_input1.bam"/>
2451333b004b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 57dfb61ba50ecfbe8f1c624fdbe2d0a3a8deaa04
iuc
parents: 3
diff changeset
512 </output>
2451333b004b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 57dfb61ba50ecfbe8f1c624fdbe2d0a3a8deaa04
iuc
parents: 3
diff changeset
513 <output name="output_feature_lengths" file="output_feature_lengths.tab">
2451333b004b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 57dfb61ba50ecfbe8f1c624fdbe2d0a3a8deaa04
iuc
parents: 3
diff changeset
514 <metadata name="column_names" value="Feature,Length"/>
2451333b004b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 57dfb61ba50ecfbe8f1c624fdbe2d0a3a8deaa04
iuc
parents: 3
diff changeset
515 </output>
0
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
516 </test>
10
7d1e37c6ef85 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
iuc
parents: 9
diff changeset
517 <test expect_num_outputs="4">
34
12b3317716d3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit fc2a03ae0ef4d1d0d5f2f05a6ecc0fc4812931e1
iuc
parents: 33
diff changeset
518 <param name="alignment" value="featureCounts_input1.bam" ftype="unsorted.bam" dbkey="hg38"/>
11
d0beb2753aba planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 4c095ddb0f2c369d2bf3fc5ca386f6981a2fa0eb
iuc
parents: 10
diff changeset
519 <param name="anno_select" value="history"/>
32
456cc2927264 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents: 31
diff changeset
520 <param name="reference_gene_sets" value="featureCounts_guide.gff" ftype="gff" dbkey="hg38"/>
456cc2927264 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents: 31
diff changeset
521 <param name="format" value="tabdel_short"/>
10
7d1e37c6ef85 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
iuc
parents: 9
diff changeset
522 <param name="include_feature_length_file" value="true"/>
7d1e37c6ef85 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
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parents: 9
diff changeset
523 <param name="count_exon_exon_junction_reads" value="-J"/>
13
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iuc
parents: 12
diff changeset
524 <output name="output_short" file="output_1_short.tab">
10
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iuc
parents: 9
diff changeset
525 <metadata name="column_names" value="Geneid,featureCounts_input1.bam"/>
7d1e37c6ef85 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
iuc
parents: 9
diff changeset
526 </output>
13
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iuc
parents: 12
diff changeset
527 <output name="output_summary" file="output_1_summary.tab">
10
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iuc
parents: 9
diff changeset
528 <metadata name="column_names" value="Status,featureCounts_input1.bam"/>
7d1e37c6ef85 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
iuc
parents: 9
diff changeset
529 </output>
13
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iuc
parents: 12
diff changeset
530 <output name="output_jcounts" file="output_1_jcounts.tab">
10
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iuc
parents: 9
diff changeset
531 <metadata name="column_names" value="PrimaryGene,SecondaryGene,Site1_chr,Site1_location,Site1_strand,Site2_chr,Site2_location,Site2_strand,featureCounts_input1.bam"/>
7d1e37c6ef85 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
iuc
parents: 9
diff changeset
532 </output>
7d1e37c6ef85 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
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parents: 9
diff changeset
533 </test>
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parents: 10
diff changeset
534 <!-- Ensure featureCounts built-in annotation works -->
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iuc
parents: 15
diff changeset
535 <test expect_num_outputs="3">
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parents: 33
diff changeset
536 <param name="alignment" value="pairend_strandspecific_51mer_hg19_chr1_1-100000.bam" ftype="unsorted.bam" dbkey="hg19"/>
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parents: 9
diff changeset
537 <param name="anno_select" value="builtin"/>
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diff changeset
538 <param name="format" value="tabdel_short"/>
456cc2927264 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents: 31
diff changeset
539 <conditional name="pe_parameters">
456cc2927264 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents: 31
diff changeset
540 <param name="paired_end_status" value="PE_individual"/>
456cc2927264 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents: 31
diff changeset
541 </conditional>
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iuc
parents: 15
diff changeset
542 <section name="extended_parameters">
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parents: 31
diff changeset
543 <param name="R" value="true"/>
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parents: 15
diff changeset
544 </section>
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iuc
parents: 9
diff changeset
545 <output name="output_short" file="output_builtin_hg19.tab">
7d1e37c6ef85 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
iuc
parents: 9
diff changeset
546 <metadata name="column_names" value="Geneid,pairend_strandspecific_51mer_hg19_chr1_1-100000.bam"/>
7d1e37c6ef85 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
iuc
parents: 9
diff changeset
547 </output>
7d1e37c6ef85 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
iuc
parents: 9
diff changeset
548 <output name="output_summary" file="output_summary_builtin_hg19.tab"/>
17
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iuc
parents: 15
diff changeset
549 <output name="output_bam" file="output.bam" ftype="bam"/>
10
7d1e37c6ef85 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit cf1ae941d02bff8848f05c4e4039457656e3a4e8
iuc
parents: 9
diff changeset
550 </test>
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iuc
parents: 32
diff changeset
551 <!-- Ensure fragment counting works -->
dad3302e7b34 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a0d90afa3160e737786cc448aaf7c92df94a64cc
iuc
parents: 32
diff changeset
552 <test expect_num_outputs="3">
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parents: 33
diff changeset
553 <param name="alignment" value="pairend_strandspecific_51mer_hg19_chr1_1-100000.bam" ftype="unsorted.bam" dbkey="hg19"/>
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parents: 32
diff changeset
554 <param name="anno_select" value="builtin"/>
dad3302e7b34 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a0d90afa3160e737786cc448aaf7c92df94a64cc
iuc
parents: 32
diff changeset
555 <param name="format" value="tabdel_short"/>
dad3302e7b34 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a0d90afa3160e737786cc448aaf7c92df94a64cc
iuc
parents: 32
diff changeset
556 <conditional name="pe_parameters">
dad3302e7b34 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a0d90afa3160e737786cc448aaf7c92df94a64cc
iuc
parents: 32
diff changeset
557 <param name="paired_end_status" value="PE_fragments"/>
dad3302e7b34 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a0d90afa3160e737786cc448aaf7c92df94a64cc
iuc
parents: 32
diff changeset
558 </conditional>
dad3302e7b34 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a0d90afa3160e737786cc448aaf7c92df94a64cc
iuc
parents: 32
diff changeset
559 <section name="extended_parameters">
dad3302e7b34 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a0d90afa3160e737786cc448aaf7c92df94a64cc
iuc
parents: 32
diff changeset
560 <param name="R" value="true"/>
dad3302e7b34 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a0d90afa3160e737786cc448aaf7c92df94a64cc
iuc
parents: 32
diff changeset
561 </section>
dad3302e7b34 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a0d90afa3160e737786cc448aaf7c92df94a64cc
iuc
parents: 32
diff changeset
562 <output name="output_short" file="output_builtin_hg19_fragment.tab">
dad3302e7b34 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a0d90afa3160e737786cc448aaf7c92df94a64cc
iuc
parents: 32
diff changeset
563 <metadata name="column_names" value="Geneid,pairend_strandspecific_51mer_hg19_chr1_1-100000.bam"/>
dad3302e7b34 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a0d90afa3160e737786cc448aaf7c92df94a64cc
iuc
parents: 32
diff changeset
564 </output>
dad3302e7b34 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a0d90afa3160e737786cc448aaf7c92df94a64cc
iuc
parents: 32
diff changeset
565 <output name="output_summary" file="output_summary_builtin_hg19_fragments.tab"/>
dad3302e7b34 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a0d90afa3160e737786cc448aaf7c92df94a64cc
iuc
parents: 32
diff changeset
566 <output name="output_bam" file="output_fragments.bam" ftype="bam"/>
dad3302e7b34 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit a0d90afa3160e737786cc448aaf7c92df94a64cc
iuc
parents: 32
diff changeset
567 </test>
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iuc
parents: 10
diff changeset
568 <!-- Ensure cached GTFs work -->
d0beb2753aba planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 4c095ddb0f2c369d2bf3fc5ca386f6981a2fa0eb
iuc
parents: 10
diff changeset
569 <test expect_num_outputs="3">
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parents: 33
diff changeset
570 <param name="alignment" value="featureCounts_input1.bam" ftype="unsorted.bam" dbkey="hg38"/>
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d0beb2753aba planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 4c095ddb0f2c369d2bf3fc5ca386f6981a2fa0eb
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parents: 10
diff changeset
571 <param name="anno_select" value="cached"/>
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parents: 31
diff changeset
572 <param name="format" value="tabdel_medium"/>
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parents: 10
diff changeset
573 <param name="include_feature_length_file" value="true"/>
d0beb2753aba planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 4c095ddb0f2c369d2bf3fc5ca386f6981a2fa0eb
iuc
parents: 10
diff changeset
574 <output name="output_medium" file="output_1_medium.tab">
d0beb2753aba planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 4c095ddb0f2c369d2bf3fc5ca386f6981a2fa0eb
iuc
parents: 10
diff changeset
575 <metadata name="column_names" value="Geneid,featureCounts_input1.bam,Length"/>
d0beb2753aba planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 4c095ddb0f2c369d2bf3fc5ca386f6981a2fa0eb
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parents: 10
diff changeset
576 </output>
d0beb2753aba planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 4c095ddb0f2c369d2bf3fc5ca386f6981a2fa0eb
iuc
parents: 10
diff changeset
577 <output name="output_summary" file="output_1_summary.tab">
d0beb2753aba planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 4c095ddb0f2c369d2bf3fc5ca386f6981a2fa0eb
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parents: 10
diff changeset
578 <metadata name="column_names" value="Status,featureCounts_input1.bam"/>
d0beb2753aba planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 4c095ddb0f2c369d2bf3fc5ca386f6981a2fa0eb
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parents: 10
diff changeset
579 </output>
d0beb2753aba planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 4c095ddb0f2c369d2bf3fc5ca386f6981a2fa0eb
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parents: 10
diff changeset
580 </test>
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parents: 17
diff changeset
581 <!-- Ensure BAM output works -->
34
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parents: 33
diff changeset
582 <test expect_num_outputs="3">
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parents: 31
diff changeset
583 <param name="alignment" value="subset.sorted.bam" ftype="bam"/>
456cc2927264 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents: 31
diff changeset
584 <param name="anno_select" value="history"/>
456cc2927264 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents: 31
diff changeset
585 <param name="reference_gene_sets" value="small.gtf" ftype="gtf"/>
456cc2927264 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
iuc
parents: 31
diff changeset
586 <section name="extended_parameters">
456cc2927264 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 2d5d24cbdea47adb6eede757016c717840749fd1
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parents: 31
diff changeset
587 <param name="R" value="true"/>
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parents: 17
diff changeset
588 </section>
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parents: 20
diff changeset
589 <output name="output_bam" value="subset.sorted.featurecounts.bam" compare="sim_size"/>
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parents: 33
diff changeset
590 <output name="output_short" file="subset.sorted.featurecounts_short.tab">
12b3317716d3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit fc2a03ae0ef4d1d0d5f2f05a6ecc0fc4812931e1
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parents: 33
diff changeset
591 <metadata name="column_names" value="Geneid,subset.sorted.bam"/>
12b3317716d3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit fc2a03ae0ef4d1d0d5f2f05a6ecc0fc4812931e1
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parents: 33
diff changeset
592 </output>
12b3317716d3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit fc2a03ae0ef4d1d0d5f2f05a6ecc0fc4812931e1
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parents: 33
diff changeset
593 <output name="output_summary" file="subset.sorted.featurecounts_summary.tab"/>
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parents: 17
diff changeset
594 </test>
0
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diff changeset
595 </tests>
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diff changeset
596
0
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diff changeset
597 <help><![CDATA[
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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diff changeset
598 featureCounts
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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parents:
diff changeset
599 #############
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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parents:
diff changeset
600
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
iuc
parents:
diff changeset
601 Overview
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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diff changeset
602 --------
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parents: 9
diff changeset
603 FeatureCounts is a light-weight read counting program written entirely in the C programming language. It can be used to count both gDNA-seq and RNA-seq reads for genomic features in in SAM/BAM files. FeatureCounts is part of the Subread_ package.
0
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604
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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diff changeset
605 Input formats
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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606 -------------
8e70555968a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/featurecounts commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
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607 Alignments should be provided in either:
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608
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609 - SAM format, http://samtools.sourceforge.net/samtools.shtml#5
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610 - BAM format
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611
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612 Annotations for gene regions should be provided in the GFF/GTF format:
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613
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614 - http://genome.ucsc.edu/FAQ/FAQformat.html#format3
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615 - http://www.ensembl.org/info/website/upload/gff.html
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616
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617 Alternatively, the featureCounts built-in annotations for genomes hg38, hg19, mm10 and mm9 can be used through selecting the built-in option above. These annotation files are in simplified annotation format (SAF) as shown below. The GeneID column contains Entrez gene identifiers and each entry (row) is taken as a feature (e.g. an exon).
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618
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619 Example - **Built-in annotation format**:
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620
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621 ====== ==== ======= ======= ======
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622 GeneID Chr Start End Strand
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623 ====== ==== ======= ======= ======
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624 497097 chr1 3204563 3207049 -
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625 497098 chr1 3411783 3411982 -
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626 497099 chr1 3660633 3661579 -
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627 ====== ==== ======= ======= ======
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628
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629 These annotation files can be found in the _`Subread package`_. You can see the version of Subread used by this wrapper in the tool form above under `Options > Requirements`. To create the files, the annotations were downloaded from NCBI RefSeq database and then adapted by merging overlapping exons from the same gene to form a set of disjoint exons for each gene. Genes with the same Entrez gene identifiers were also merged into one gene. See the `Subread User's Guide`_ for more information. Gene names can be obtained for these Entrez identifiers with the Galaxy **annotateMyIDs** tool.
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630
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631 Output format
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632 -------------
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633 FeatureCounts produces a table containing counted reads, per gene, per row. Optionally the last column can be set to be the effective gene-length. These tables are compatible with the DESeq2, edgeR and limma-voom Galaxy wrappers by IUC.
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634
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635 .. _Subread: http://subread.sourceforge.net/
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636 .. _`Subread User's Guide`: https://bioconductor.org/packages/release/bioc/vignettes/Rsubread/inst/doc/SubreadUsersGuide.pdf
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637 ]]></help>
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638 <citations>
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639 <citation type="doi">10.1093/bioinformatics/btt656</citation>
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640 </citations>
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641 </tool>
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642