Mercurial > repos > iuc > ena_webin_cli
comparison ena_webin_cli.xml @ 0:7f669682f4ac draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/ena_webin_cli commit abb15194a196267142d88b9348facf9e85e601ef
| author | iuc |
|---|---|
| date | Mon, 06 Oct 2025 12:13:07 +0000 |
| parents | |
| children | 1090ae5e7b29 |
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| -1:000000000000 | 0:7f669682f4ac |
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| 1 <tool id="ena_webin_cli" name="ENA Webin CLI" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" license="MIT" profile="24.2"> | |
| 2 <description>Submission of consensus sequences to the European Nucleotide Archive (ENA)</description> | |
| 3 <macros> | |
| 4 <token name="@TOOL_VERSION@">9.0.1</token> | |
| 5 <token name="@VERSION_SUFFIX@">0</token> | |
| 6 </macros> | |
| 7 <requirements> | |
| 8 <requirement type="package" version="@TOOL_VERSION@">ena-webin-cli</requirement> | |
| 9 <requirement type="package" version="5.3">pyyaml</requirement> | |
| 10 </requirements> | |
| 11 | |
| 12 <command detect_errors="exit_code"><![CDATA[ | |
| 13 ## ----------------------------------------------------------------------------- | |
| 14 ## 1) Initialize log and credentials | |
| 15 ## ----------------------------------------------------------------------------- | |
| 16 | |
| 17 ## Truncate (or create) the Galaxy-captured Webin-CLI log dataset. | |
| 18 : > "$webin_cli_log"; | |
| 19 | |
| 20 ## Default path where the <configfile name="credentials"> content will be written. | |
| 21 ## In dry-run we override this with a fake file so tests don’t require real creds. | |
| 22 #set $credentials = 'credentials' | |
| 23 #if $dry_run == "true": | |
| 24 #set $credentials = './test_fake_path' | |
| 25 touch $credentials; | |
| 26 echo "username:test_user" >> "$credentials"; | |
| 27 echo "password:test_password" >> "$credentials"; | |
| 28 #end if | |
| 29 | |
| 30 ## Extract username/password from the credentials file. | |
| 31 ## NOTE: $webin_id appears in bash, so we escape it as \$webin_id. | |
| 32 webin_id=`grep 'username' $credentials | cut -d':' -f2,2`; | |
| 33 if [ -z "\$webin_id" ]; then | |
| 34 ## Fail early with guidance if no username is present. | |
| 35 echo "No ENA credentials defined. Set your credentials via: User -> Preferences -> Manage Information" >&2; | |
| 36 exit 1; | |
| 37 else | |
| 38 ## Pull the password similarly. | |
| 39 password=`grep 'password' $credentials | cut -d':' -f2,2`; | |
| 40 fi; | |
| 41 | |
| 42 ## ----------------------------------------------------------------------------- | |
| 43 ## 2) Create a base manifest (fields common to all submissions) | |
| 44 ## ----------------------------------------------------------------------------- | |
| 45 | |
| 46 ## Name of the base manifest template (we append more fields later). | |
| 47 #set $manifest_base = 'manifest_base.tab' | |
| 48 | |
| 49 ## Working dirs: per-sample manifests and input sequences. | |
| 50 mkdir -p manifests; | |
| 51 mkdir -p fasta; | |
| 52 | |
| 53 ## Write assembly-level fields to the base manifest. | |
| 54 echo -e 'ASSEMBLY_TYPE\t$assembly_type' > $manifest_base; | |
| 55 echo -e 'COVERAGE\t$coverage' >> $manifest_base; | |
| 56 echo -e 'PROGRAM\t$assembly_program' >> $manifest_base; | |
| 57 #if $min_gap_length: | |
| 58 echo -e 'MINGAPLENGTH\t$min_gap_length' >> $manifest_base; | |
| 59 #end if | |
| 60 echo -e 'MOLECULETYPE\t$molecule_type' >> $manifest_base; | |
| 61 | |
| 62 ## ----------------------------------------------------------------------------- | |
| 63 ## 3) Build per-sample manifests depending on metadata workflow | |
| 64 ## ----------------------------------------------------------------------------- | |
| 65 | |
| 66 #if $metadata_file_or_form.metadata_format == "file": | |
| 67 ## --------------------------- FILE-DRIVEN WORKFLOW ------------------------ | |
| 68 ## For each selected FASTA: | |
| 69 ## - if uncompressed (.fasta), gzip it into ./fasta/<name>.fasta.gz | |
| 70 ## - if already .fasta.gz, symlink it into ./fasta | |
| 71 #import re | |
| 72 #for $file in $metadata_file_or_form.genome_fasta: | |
| 73 #if $file.is_of_type('fasta'): | |
| 74 #set $full_name = $file.element_identifier + '.gz' | |
| 75 gzip -c '$file' > './fasta/$full_name'; | |
| 76 #else: | |
| 77 ln -s '$file' './fasta/$file.element_identifier'; | |
| 78 #end if | |
| 79 #end for | |
| 80 | |
| 81 ## Optionally link AGP and chromosome list files if provided (one per sample). | |
| 82 #if $metadata_file_or_form.agp_file: | |
| 83 #for $file in $metadata_file_or_form.agp_file: | |
| 84 ln -s '$file' './fasta/$file.element_identifier'; | |
| 85 #end for | |
| 86 #end if | |
| 87 #if $metadata_file_or_form.chr_list_file: | |
| 88 #for $file in $metadata_file_or_form.chr_list_file: | |
| 89 #set $chr_name = $file.element_identifier + '.gz' | |
| 90 gzip -c '$file' > './fasta/$chr_name'; | |
| 91 #end for | |
| 92 #end if | |
| 93 | |
| 94 ## Helper script: | |
| 95 ## - parses ENA receipt (study/sample accessions, platform), | |
| 96 ## - writes per-sample manifests into ./manifests using $manifest_base, | |
| 97 ## - emits "submit_list.tab" (one manifest path per line). | |
| 98 python3 '$__tool_directory__/process_input.py' $metadata_file_or_form.ena_receipt $genome_fasta_files './manifests' $manifest_base >> "$webin_cli_log" 2>&1; | |
| 99 | |
| 100 ## Extract center name from the receipt (used as -centerName). | |
| 101 center_name=`grep 'center_name' $metadata_file_or_form.ena_receipt | cut -f2,2 | tr -d '\n'`; | |
| 102 | |
| 103 ## Log if submit_list.tab exists, and dump its content for debugging. | |
| 104 if [ -s submit_list.tab ]; then | |
| 105 echo "submit_list.tab present:" >> "$webin_cli_log" 2>&1; | |
| 106 cat submit_list.tab >> "$webin_cli_log" 2>&1; | |
| 107 else | |
| 108 echo "submit_list.tab is missing or empty" >> "$webin_cli_log" 2>&1; | |
| 109 fi; | |
| 110 | |
| 111 #else: | |
| 112 ## --------------------------- FORM-DRIVEN WORKFLOW ------------------------ | |
| 113 ## Single-manifest flow: copy base, then append form fields. | |
| 114 #set $generated_manifest='./manifests/generated_manifest.txt' | |
| 115 cp $manifest_base $generated_manifest; | |
| 116 | |
| 117 ## Use local Cheetah vars for readability. | |
| 118 #set $study_id = $metadata_file_or_form.study_accession | |
| 119 #set $sample_id = $metadata_file_or_form.sample_accession | |
| 120 | |
| 121 ## Required accessions. | |
| 122 echo -e 'STUDY\t$study_id' >> $generated_manifest; | |
| 123 echo -e 'SAMPLE\t$sample_id' >> $generated_manifest; | |
| 124 | |
| 125 ## center_name is given by the user in this path. | |
| 126 center_name='$metadata_file_or_form.center_name'; | |
| 127 | |
| 128 ## Assembly name and platform. | |
| 129 echo -e 'NAME\t$metadata_file_or_form.assembly_name' >> $generated_manifest; | |
| 130 echo -e 'PLATFORM\t$metadata_file_or_form.sequencing_platform' >> $generated_manifest; | |
| 131 | |
| 132 ## Normalize FASTA name referenced by the manifest. | |
| 133 #if $metadata_file_or_form.genome_fasta.is_of_type('fasta'): | |
| 134 #set $fasta_file_name = $metadata_file_or_form.genome_fasta.element_identifier + '.gz' | |
| 135 gzip -c '$metadata_file_or_form.genome_fasta' > $fasta_file_name; | |
| 136 #else: | |
| 137 #set $fasta_file_name = $metadata_file_or_form.genome_fasta.element_identifier | |
| 138 #end if | |
| 139 echo -e 'FASTA\t$fasta_file_name' >> $generated_manifest; | |
| 140 | |
| 141 ## Optional extras for chromosome-scale assemblies. | |
| 142 #if $metadata_file_or_form.agp_file: | |
| 143 echo -e 'AGP\t$metadata_file_or_form.agp_file.element_identifier' >> $generated_manifest; | |
| 144 #end if | |
| 145 #if $metadata_file_or_form.chr_list_file: | |
| 146 ## If the name ends with .tsv, gzip it and reference the .gz; else use as-is. | |
| 147 #set $chr_file_name = $metadata_file_or_form.chr_list_file.element_identifier + '.gz' | |
| 148 gzip -c '$metadata_file_or_form.chr_list_file' > $chr_file_name; | |
| 149 echo -e 'CHROMOSOME_LIST\t$chr_file_name' >> $generated_manifest; | |
| 150 #end if | |
| 151 #end if | |
| 152 | |
| 153 ## ----------------------------------------------------------------------------- | |
| 154 ## 4) Prepare output directory and build CLI flags safely with Cheetah | |
| 155 ## ----------------------------------------------------------------------------- | |
| 156 | |
| 157 ## Webin-CLI will write receipts/logs under this directory (we later tar it). | |
| 158 #set $outputs_dir = 'outputs' | |
| 159 mkdir -p "$outputs_dir"; | |
| 160 | |
| 161 ## Build flags using #set (safer than inline #if within a single shell line). | |
| 162 #set $test_flag = '' | |
| 163 #if $submit_test == "true": | |
| 164 #set $test_flag = ' -test' | |
| 165 #end if | |
| 166 | |
| 167 ## By default we submit; in dry_run we validate instead. | |
| 168 #set $action_flag = ' -submit' | |
| 169 #if $dry_run == "true": | |
| 170 #set $action_flag = ' -validate' | |
| 171 #end if | |
| 172 | |
| 173 ## ----------------------------------------------------------------------------- | |
| 174 ## 5) Execute Webin-CLI | |
| 175 ## ----------------------------------------------------------------------------- | |
| 176 | |
| 177 #if $metadata_file_or_form.metadata_format == "file": | |
| 178 ## Loop over each manifest written by process_input.py (submit_list.tab). | |
| 179 while IFS= read -r line; do | |
| 180 ## Extract the manifest path (first whitespace-delimited field). | |
| 181 manifest=`echo "\$line" | cut -d' ' -f1`; | |
| 182 | |
| 183 ## Log which manifest we are submitting. | |
| 184 echo "Submitting manifest \$manifest" >> "$webin_cli_log" 2>&1; | |
| 185 | |
| 186 ## Invoke Webin-CLI with computed flags. | |
| 187 ena-webin-cli -context genome -manifest "\$manifest" -userName "'\$webin_id'" -password "'\$password'" -centerName "'\$center_name'" -inputDir './fasta' $test_flag $action_flag -outputDir $outputs_dir >> '$webin_cli_log' 2>&1 || true; | |
| 188 done < submit_list.tab; | |
| 189 | |
| 190 #else: | |
| 191 ## Single run in "form" mode with the one generated manifest. | |
| 192 ena-webin-cli -context genome -manifest $generated_manifest -userName "'\$webin_id'" -password "'\$password'" -centerName "'\$center_name'" -inputDir ./ $test_flag $action_flag -outputDir $outputs_dir >> "$webin_cli_log" 2>&1 || true; | |
| 193 #end if | |
| 194 | |
| 195 ## ----------------------------------------------------------------------------- | |
| 196 ## 6) Package outputs for Galaxy | |
| 197 ## ----------------------------------------------------------------------------- | |
| 198 | |
| 199 ## Tar up the Webin-CLI output directory so Galaxy can collect a single dataset. | |
| 200 tar -cf $webin_cli_outputs $outputs_dir ; | |
| 201 ]]></command> | |
| 202 | |
| 203 <!-- | |
| 204 Config files rendered by Galaxy *before* the command runs. | |
| 205 They are plain text files placed in the job working directory and referenced above. | |
| 206 --> | |
| 207 <configfiles> | |
| 208 <!-- Credentials file: | |
| 209 Pulls stored ENA Webin details (if set) from the Galaxy user preferences and writes | |
| 210 simple "username:..." and "password:..." lines. The command reads from this file. --> | |
| 211 <configfile name="credentials"><![CDATA[ | |
| 212 #set $webin_id = $__user__.extra_preferences.get('ena_webin_account|webin_id', "").strip() | |
| 213 #set $password = $__user__.extra_preferences.get('ena_webin_account|password', "").strip() | |
| 214 #if $webin_id != "": | |
| 215 username:$webin_id | |
| 216 password:$password | |
| 217 #end if | |
| 218 ]]></configfile> | |
| 219 | |
| 220 <!-- genome_fasta_files: | |
| 221 In "file" mode, build a JSON array containing the *element_identifier* (dataset name) | |
| 222 for each selected FASTA. process_input.py uses these names to derive sample aliases. --> | |
| 223 <configfile name="genome_fasta_files"> | |
| 224 #import json | |
| 225 #import re | |
| 226 #if $metadata_file_or_form.metadata_format == "file": | |
| 227 #set $fasta_files_list = list() | |
| 228 #for $file in $metadata_file_or_form.genome_fasta: | |
| 229 $fasta_files_list.append(str($file.element_identifier)) | |
| 230 #end for | |
| 231 #echo json.dumps($fasta_files_list) | |
| 232 #end if | |
| 233 </configfile> | |
| 234 </configfiles> | |
| 235 | |
| 236 <!-- | |
| 237 User-facing inputs: | |
| 238 - Assembly-level parameters | |
| 239 - Choice of metadata workflow (file vs form) with corresponding fields | |
| 240 - Submission toggles for ENA test server and validation-only | |
| 241 --> | |
| 242 <inputs> | |
| 243 <param name="assembly_type" type="select" label="Assembly type"> | |
| 244 <option value="clone">Clone</option> | |
| 245 <option value="isolate">Isolate</option> | |
| 246 <option value="COVID-19 outbreak">COVID-19 outbreak</option> | |
| 247 </param> | |
| 248 <param name="assembly_program" type="text" optional="False" label="Assembly program"/> | |
| 249 <param name="molecule_type" type="select" label="Molecule type"> | |
| 250 <option value="genomic RNA" selected="True">genomic RNA</option> | |
| 251 <option value="viral cRNA">viral cRNA</option> | |
| 252 <option value="genomic DNA">genomic DNA</option> | |
| 253 </param> | |
| 254 <param name="coverage" type="float" optional="False" value="10000" label="Coverage"/> | |
| 255 <param name="min_gap_length" type="text" optional="True" label="Minimum gap length (optional)"/> | |
| 256 | |
| 257 <conditional name="metadata_file_or_form"> | |
| 258 <param name="metadata_format" type="select" label="Select the method to load study and sample metadata"> | |
| 259 <option value="form" selected="True">Fill in required submission metadata</option> | |
| 260 <option value="file">I used the Galaxy ENA upload tool to submit the raw data: parse the received submission receipt</option> | |
| 261 </param> | |
| 262 | |
| 263 <!-- FILE workflow: receipt + multiple FASTA (+ optional AGP/TSV) --> | |
| 264 <when value="file"> | |
| 265 <param type="data" format="txt" name="ena_receipt" optional="False" label="Submission receipt obtained from ENA upload tool"/> | |
| 266 <param name="genome_fasta" type="data" optional="False" label="Select the consensus sequence assembly files or a collection of them. Use following syntax: sample_alias.fasta or sample_alias.fasta.gz" format="fasta,fasta.gz" multiple="true"/> | |
| 267 <param name="agp_file" type="data" optional="True" label="Sequences in AGP format. Use following syntax: sample_alias.agp" format="agp" multiple="true"/> | |
| 268 <param name="chr_list_file" type="data" optional="True" label="Chromosome List File, must be provided when the submission contains assembled chromosomes. Use following syntax: sample_alias.tsv" format="tsv" multiple="true"/> | |
| 269 </when> | |
| 270 | |
| 271 <!-- FORM workflow: single, user-specified submission --> | |
| 272 <when value="form"> | |
| 273 <param name="assembly_name" type="text" optional="False" label="Assembly name"/> | |
| 274 <param name="study_accession" type="text" optional="False" label="Study accession"/> | |
| 275 <param name="sample_accession" type="text" optional="False" label="Sample accession"/> | |
| 276 <param name="sequencing_platform" type="text" optional="False" label="Sequencing platform"/> | |
| 277 <param name="description" type="text" optional="True" value="" label="Description" help="Free text description of the genome assembly (optional)"/> | |
| 278 <param name="center_name" type="text" optional="False" label="Center name"/> | |
| 279 <param name="genome_fasta" type="data" optional="False" label="Select the consensus sequence assembly file" format="fasta,fasta.gz"/> | |
| 280 <param name="agp_file" type="data" optional="True" label="Sequences in AGP format." format="agp"/> | |
| 281 <param name="chr_list_file" type="data" optional="True" label="Chromosome List File, must be provided when the submission contains assembled chromosomes." format="tsv"/> | |
| 282 </when> | |
| 283 </conditional> | |
| 284 | |
| 285 <!-- Submission controls --> | |
| 286 <param name="submit_test" type="boolean" truevalue="true" falsevalue="false" label="Submit to ENA test server" help="Uploads to the test server of ENA will not be made public and will be removed automatically in 24 hours. Performing a preliminary test upload is advised to check for errors with metadata structure. You can find these uploads at https://wwwdev.ebi.ac.uk/ena/." /> | |
| 287 <param name="dry_run" type="boolean" truevalue="true" falsevalue="false" label="Validate files and metadata but do not submit" help="Generate input files and run Webin-CLI with -validate option. If 'No' is selected then it will validate and submit (-submit flag)"/> | |
| 288 </inputs> | |
| 289 | |
| 290 <!-- | |
| 291 Outputs: | |
| 292 - generated_manifests: discovered in manifests/ (via regex) for transparency | |
| 293 - webin_cli_log: combined stdout/stderr + helper echo statements | |
| 294 - webin_cli_outputs: tar archive of the Webin-CLI output directory | |
| 295 --> | |
| 296 <outputs> | |
| 297 <collection name="generated_manifests" type="list" label="Generated manifests"> | |
| 298 <discover_datasets pattern="(?P<designation>.+)\.txt" ext="txt" directory="manifests/"/> | |
| 299 </collection> | |
| 300 <data name="webin_cli_log" label="ENA submission log" format="txt"/> | |
| 301 <data name="webin_cli_outputs" label="Webin cli outputs" format="tar"/> | |
| 302 | |
| 303 </outputs> | |
| 304 | |
| 305 <tests> | |
| 306 <!-- Test 1: FORM workflow, no chr/AGP --> | |
| 307 <test> | |
| 308 <param name="submit_test" value="true" /> | |
| 309 <param name="dry_run" value="true" /> | |
| 310 <param name="assembly_type" value="isolate"/> | |
| 311 <param name="assembly_program" value="Test assembly program"/> | |
| 312 <param name="molecule_type" value="viral cRNA"/> | |
| 313 <param name="coverage" value="10000"/> | |
| 314 <conditional name="metadata_file_or_form"> | |
| 315 <param name="metadata_format" value="form"/> | |
| 316 <param name="assembly_name" value="Test assembly name"/> | |
| 317 <param name="study_accession" value="PRJEB49173"/> | |
| 318 <param name="sample_accession" value="SAMEA11953908"/> | |
| 319 <param name="sequencing_platform" value="Nanopore 0011"/> | |
| 320 <param name="description" value="Test Description"/> | |
| 321 <param name="center_name" value="Test center name"/> | |
| 322 <param name="genome_fasta" value="phiX2.fasta"/> | |
| 323 </conditional> | |
| 324 <param name="min_gap_length" value="30"/> | |
| 325 <output name="webin_cli_log"> | |
| 326 <assert_contents> | |
| 327 <has_text_matching expression="ERROR: Invalid submission account user name or password\."/> | |
| 328 </assert_contents> | |
| 329 </output> | |
| 330 <output_collection name="generated_manifests"> | |
| 331 <element name="generated_manifest"> | |
| 332 <assert_contents> | |
| 333 <has_text_matching expression="(?m)^FASTA\tphiX2\.fasta\.gz$"/> | |
| 334 <has_text_matching expression="(?m)^CHROMOSOME_LIST\t" negate="true"/> | |
| 335 <has_text_matching expression="(?m)^AGP\t" negate="true"/> | |
| 336 </assert_contents> | |
| 337 </element> | |
| 338 </output_collection> | |
| 339 </test> | |
| 340 | |
| 341 <!-- Test 2: FORM workflow, chr list present --> | |
| 342 <test> | |
| 343 <param name="submit_test" value="true" /> | |
| 344 <param name="dry_run" value="true" /> | |
| 345 <param name="assembly_type" value="isolate"/> | |
| 346 <param name="assembly_program" value="Test assembly program"/> | |
| 347 <param name="molecule_type" value="genomic DNA"/> | |
| 348 <param name="coverage" value="10000"/> | |
| 349 <conditional name="metadata_file_or_form"> | |
| 350 <param name="metadata_format" value="form"/> | |
| 351 <param name="assembly_name" value="Test assembly name"/> | |
| 352 <param name="study_accession" value="PRJEB49173"/> | |
| 353 <param name="sample_accession" value="SAMEA11953908"/> | |
| 354 <param name="sequencing_platform" value="Nanopore 0011"/> | |
| 355 <param name="description" value="Test Description"/> | |
| 356 <param name="center_name" value="Test center name"/> | |
| 357 <param name="genome_fasta" value="phiX3.fasta"/> | |
| 358 <param name="chr_list_file" value="phiX3.tsv"/> | |
| 359 </conditional> | |
| 360 <param name="min_gap_length" value="30"/> | |
| 361 <output name="webin_cli_log"> | |
| 362 <assert_contents> | |
| 363 <has_text_matching expression="ERROR: Invalid submission account user name or password\."/> | |
| 364 </assert_contents> | |
| 365 </output> | |
| 366 <output_collection name="generated_manifests"> | |
| 367 <element name="generated_manifest"> | |
| 368 <assert_contents> | |
| 369 <has_text_matching expression="(?m)^FASTA\tphiX3\.fasta\.gz$"/> | |
| 370 <has_text_matching expression="(?m)^CHROMOSOME_LIST\tphiX3\.tsv\.gz$"/> | |
| 371 <has_text_matching expression="(?m)^AGP\t" negate="true"/> | |
| 372 </assert_contents> | |
| 373 </element> | |
| 374 </output_collection> | |
| 375 </test> | |
| 376 | |
| 377 <!-- Test 3: FILE workflow, two FASTAs; one missing metadata --> | |
| 378 <test> | |
| 379 <param name="submit_test" value="true" /> | |
| 380 <param name="dry_run" value="true" /> | |
| 381 <param name="assembly_type" value="isolate"/> | |
| 382 <param name="assembly_program" value="Test assembly program"/> | |
| 383 <param name="molecule_type" value="viral cRNA"/> | |
| 384 <param name="coverage" value="10000"/> | |
| 385 <conditional name="metadata_file_or_form"> | |
| 386 <param name="metadata_format" value="file"/> | |
| 387 <param name="ena_receipt" value="receipt_sample_nophiX2.txt"/> | |
| 388 <param name="genome_fasta" value="phiX2.fasta.gz,sample_alias_001.fasta.gz"/> | |
| 389 </conditional> | |
| 390 <param name="min_gap_length" value="30"/> | |
| 391 <output name="webin_cli_log"> | |
| 392 <assert_contents> | |
| 393 <has_text_matching expression="Processing phiX2"/> | |
| 394 <has_text_matching expression="No metadata found for sample phiX2"/> | |
| 395 <has_text_matching expression="Processing sample_alias_001"/> | |
| 396 <has_text_matching expression="Submitting manifest .*manifests/sample_alias_001\.manifest\.txt"/> | |
| 397 <has_text_matching expression="ERROR: Invalid submission account user name or password\. Please try enclosing your password in single quotes\."/> | |
| 398 </assert_contents> | |
| 399 </output> | |
| 400 <output_collection name="generated_manifests"> | |
| 401 <element name="sample_alias_001.manifest"> | |
| 402 <assert_contents> | |
| 403 <has_text_matching expression="(?m)^FASTA\tsample_alias_001\.fasta\.gz$"/> | |
| 404 <has_text_matching expression="(?m)^CHROMOSOME_LIST\t" negate="true"/> | |
| 405 <has_text_matching expression="(?m)^AGP\t" negate="true"/> | |
| 406 </assert_contents> | |
| 407 </element> | |
| 408 </output_collection> | |
| 409 </test> | |
| 410 | |
| 411 <!-- Test 4: FILE workflow, single FASTA with metadata --> | |
| 412 <test> | |
| 413 <param name="submit_test" value="true" /> | |
| 414 <param name="dry_run" value="true" /> | |
| 415 <param name="assembly_type" value="isolate"/> | |
| 416 <param name="assembly_program" value="Test assembly program"/> | |
| 417 <param name="molecule_type" value="viral cRNA"/> | |
| 418 <param name="coverage" value="10000"/> | |
| 419 <conditional name="metadata_file_or_form"> | |
| 420 <param name="metadata_format" value="file"/> | |
| 421 <param name="ena_receipt" value="receipt_sample.txt"/> | |
| 422 <param name="genome_fasta" value="sample_alias_001.fasta.gz"/> | |
| 423 </conditional> | |
| 424 <param name="min_gap_length" value="30"/> | |
| 425 <output name="webin_cli_log"> | |
| 426 <assert_contents> | |
| 427 <has_text_matching expression="Processing sample_alias_001"/> | |
| 428 <has_text_matching expression="ERROR: Invalid submission account user name or password\. Please try enclosing your password in single quotes\."/> | |
| 429 </assert_contents> | |
| 430 </output> | |
| 431 </test> | |
| 432 | |
| 433 <!-- Test 5: FILE workflow, AGP for phiX2 --> | |
| 434 <test> | |
| 435 <param name="submit_test" value="true" /> | |
| 436 <param name="dry_run" value="true" /> | |
| 437 <param name="assembly_type" value="isolate"/> | |
| 438 <param name="assembly_program" value="Test assembly program"/> | |
| 439 <param name="molecule_type" value="genomic DNA"/> | |
| 440 <param name="coverage" value="10000"/> | |
| 441 <conditional name="metadata_file_or_form"> | |
| 442 <param name="metadata_format" value="file"/> | |
| 443 <param name="ena_receipt" value="receipt_sample.txt"/> | |
| 444 <param name="genome_fasta" value="phiX2.fasta"/> | |
| 445 <param name="agp_file" value="phiX2.agp"/> | |
| 446 </conditional> | |
| 447 <param name="min_gap_length" value="30"/> | |
| 448 <output name="webin_cli_log"> | |
| 449 <assert_contents> | |
| 450 <has_text_matching expression="Processing phiX2"/> | |
| 451 <has_text_matching expression="ERROR: Invalid submission account user name or password\. Please try enclosing your password in single quotes\."/> | |
| 452 </assert_contents> | |
| 453 </output> | |
| 454 <output_collection name="generated_manifests"> | |
| 455 <element name="phiX2.manifest"> | |
| 456 <assert_contents> | |
| 457 <has_text_matching expression="(?m)^FASTA\tphiX2\.fasta\.gz$"/> | |
| 458 <has_text_matching expression="(?m)^AGP\tphiX2\.agp$"/> | |
| 459 <has_text_matching expression="(?m)^CHROMOSOME_LIST\t" negate="true"/> | |
| 460 </assert_contents> | |
| 461 </element> | |
| 462 </output_collection> | |
| 463 </test> | |
| 464 | |
| 465 <!-- Test 6: FILE workflow, chr list for phiX3 + extra fasta --> | |
| 466 <test> | |
| 467 <param name="submit_test" value="true" /> | |
| 468 <param name="dry_run" value="true" /> | |
| 469 <param name="assembly_type" value="isolate"/> | |
| 470 <param name="assembly_program" value="Test assembly program"/> | |
| 471 <param name="molecule_type" value="genomic DNA"/> | |
| 472 <param name="coverage" value="10000"/> | |
| 473 <conditional name="metadata_file_or_form"> | |
| 474 <param name="metadata_format" value="file"/> | |
| 475 <param name="ena_receipt" value="receipt_sample_phiX3.txt"/> | |
| 476 <param name="genome_fasta" value="phiX3.fasta,phiX2.fasta.gz"/> | |
| 477 <param name="chr_list_file" value="phiX3.tsv"/> | |
| 478 </conditional> | |
| 479 <param name="min_gap_length" value="30"/> | |
| 480 <output name="webin_cli_log"> | |
| 481 <assert_contents> | |
| 482 <has_text_matching expression="Processing phiX3"/> | |
| 483 <has_text_matching expression="ERROR: Invalid submission account user name or password\. Please try enclosing your password in single quotes\."/> | |
| 484 </assert_contents> | |
| 485 </output> | |
| 486 <output_collection name="generated_manifests"> | |
| 487 <element name="phiX2.manifest"> | |
| 488 <assert_contents> | |
| 489 <has_text_matching expression="(?m)^FASTA\tphiX2\.fasta\.gz$"/> | |
| 490 <has_text_matching expression="(?m)^CHROMOSOME_LIST\t" negate="true"/> | |
| 491 <has_text_matching expression="(?m)^AGP\t" negate="true"/> | |
| 492 </assert_contents> | |
| 493 </element> | |
| 494 <element name="phiX3.manifest"> | |
| 495 <assert_contents> | |
| 496 <has_text_matching expression="(?m)^FASTA\tphiX3\.fasta\.gz$"/> | |
| 497 <has_text_matching expression="(?m)^CHROMOSOME_LIST\tphiX3\.tsv\.gz$"/> | |
| 498 <has_text_matching expression="(?m)^AGP\t" negate="true"/> | |
| 499 </assert_contents> | |
| 500 </element> | |
| 501 </output_collection> | |
| 502 </test> | |
| 503 </tests> | |
| 504 | |
| 505 | |
| 506 | |
| 507 <!-- Help text + citation --> | |
| 508 <help><![CDATA[ | |
| 509 This tool is a wrapper for the ENA Webin CLI submission tool (https://ena-docs.readthedocs.io/en/latest/submit/general-guide/webin-cli.html). | |
| 510 | |
| 511 .. class:: warningmark | |
| 512 | |
| 513 The ENA upload tool won't work unless you have provided an ENA Webin ID in User > Preferences > Manage Information > ENA Webin account details.]]></help> | |
| 514 | |
| 515 <citations> | |
| 516 <citation type="doi">10.1093/nar/gkac1051</citation> | |
| 517 </citations> | |
| 518 </tool> |
