annotate edger.xml @ 16:09965c918573 draft default tip

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 96699cdbdadf0a1c40fdbd87f870b47049c0449f
author iuc
date Wed, 15 Oct 2025 12:56:21 +0000
parents 119f41ec0ada
children
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1 <tool id="edger" name="edgeR" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@">
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2 <description>
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3 Perform differential expression of count data
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4 </description>
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5 <macros>
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6 <token name="@TOOL_VERSION@">3.36.0</token>
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7 <token name="@VERSION_SUFFIX@">7</token>
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8 </macros>
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9 <edam_topics>
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10 <edam_topic>topic_3308</edam_topic>
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11 </edam_topics>
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12 <edam_operations>
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13 <edam_operation>operation_3563</edam_operation>
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14 <edam_operation>operation_3223</edam_operation>
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15 </edam_operations>
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16 <xrefs>
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17 <xref type="bio.tools">edger</xref>
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18 <xref type="bioconductor">edger</xref>
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19 </xrefs>
0
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20 <requirements>
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21 <requirement type="package" version="@TOOL_VERSION@">bioconductor-edger</requirement>
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22 <requirement type="package" version="3.50.0">bioconductor-limma</requirement>
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23 <requirement type="package" version="0.2.20">r-rjson</requirement>
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24 <requirement type="package" version="1.20.3">r-getopt</requirement>
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25 <requirement type="package" version="1.4.36">r-statmod</requirement>
0
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26 <!-- required for alpha function used with plotMD -->
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27 <requirement type="package" version="1.1.1">r-scales</requirement>
0
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28 </requirements>
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29 <version_command><![CDATA[
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30 echo $(R --version | grep version | grep -v GNU)", edgeR version" $(R --vanilla --slave -e "library(edgeR); cat(sessionInfo()\$otherPkgs\$edgeR\$Version)" 2> /dev/null | grep -v -i "WARNING: ")", limma version" $(R --vanilla --slave -e "library(limma); cat(sessionInfo()\$otherPkgs\$limma\$Version)" 2> /dev/null | grep -v -i "WARNING: ")", scales version" $(R --vanilla --slave -e "library(scales); cat(sessionInfo()\$otherPkgs\$scales\$Version)" 2> /dev/null | grep -v -i "WARNING: ")", rjson version" $(R --vanilla --slave -e "library(rjson); cat(sessionInfo()\$otherPkgs\$rjson\$Version)" 2> /dev/null | grep -v -i "WARNING: ")", getopt version" $(R --vanilla --slave -e "library(getopt); cat(sessionInfo()\$otherPkgs\$getopt\$Version)" 2> /dev/null | grep -v -i "WARNING: ")
0
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31 ]]></version_command>
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32 <command detect_errors="exit_code"><![CDATA[
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33 #import json
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34 Rscript '$__tool_directory__/edger.R'
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35
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36 -R '$outReport'
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37 -o '$outReport.files_path'
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38
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39 #if $input.format=="files":
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40
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41 ## Adapted from DESeq2 wrapper
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42 #set $temp_factor_names = list()
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43 #for $fact in $input.rep_factor:
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44 #set $temp_factor = list()
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45 #for $g in $fact.rep_group:
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46 #set $count_files = list()
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47 #for $file in $g.countsFile:
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48 $count_files.append(str($file))
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49 #end for
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50 $temp_factor.append( {str($g.groupName): $count_files} )
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51 #end for
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52
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53 $temp_factor.reverse()
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54 $temp_factor_names.append([str($fact.factorName), $temp_factor])
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55 #end for
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56 -j '#echo json.dumps(temp_factor_names)#'
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57
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58 #elif $input.format=="matrix":
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59 -m '$input.counts'
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60 #if $input.fact.ffile=='yes':
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61 -f '$input.fact.finfo'
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62 #else:
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63 -i '${ '|'.join( ['%s::%s' % ($x.factorName, $x.groupNames) for x in $input.fact.rep_factor] ) }'
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64 #end if
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65 #end if
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66
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67 #if $anno.annoOpt=='yes':
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68 -a '$anno.geneanno'
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69 #end if
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70
14
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71 #if $formula:
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72 -F '$formula'
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73 #end if
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74
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75 #if $contrasts.contrastOpt == 'file':
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76 -C '$contrasts.cinfo'
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77 #else:
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78 -C '${ ','.join( ['%s' % $x.contrast for x in $contrasts.rep_contrast] ) }'
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79 #end if
0
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80
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81 #if $f.filt.filt_select == 'yes':
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82 #if $f.filt.cformat.format_select == 'cpm':
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83 -c '$f.filt.cformat.cpmReq'
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84 -s '$f.filt.cformat.cpmSampleReq'
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85 #elif $f.filt.cformat.format_select == 'counts':
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86 -z '$f.filt.cformat.cntReq'
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87 #if $f.filt.cformat.samples.count_select == 'total':
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88 -y
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89 #elif $f.filt.cformat.samples.count_select == 'sample':
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90 -s '$f.filt.cformat.samples.cntSampleReq'
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91 #end if
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92 #end if
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93 #end if
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94
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95 #if $out.normCounts:
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96 -x
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97 #end if
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98 #if $out.rdaOption:
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99 -r
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100 #end if
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101
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102 -l '$adv.lfc'
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103 -p '$adv.pVal'
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104 -d '$adv.pAdjust'
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105 -n '$adv.normalisationOption'
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106 #if $adv.robOption:
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107 -b
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108 #end if
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109 #if $adv.lrtOption:
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110 -t
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111 #end if
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112
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113 &&
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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114 mkdir ./output_dir
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115
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116 &&
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117 cp '$outReport.files_path'/*.tsv output_dir/
2
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parents: 1
diff changeset
118
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119 #if $out.rscript:
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120 && cp '$__tool_directory__/edger.R' '$rscript'
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121 #end if
0
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122 ]]></command>
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123 <inputs>
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124 <!-- Counts and Factors -->
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125 <conditional name="input">
12
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126 <param name="format" type="select" label="Count Files or Matrix?" help="You can choose to input either separate count files (one per sample) or a single count matrix">
0
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127 <option value="files">Separate Count Files</option>
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128 <option value="matrix">Single Count Matrix</option>
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129 </param>
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130 <when value="files">
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131 <repeat name="rep_factor" title="Factor" min="1">
6
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132 <param name="factorName" type="text" label="Name" help="Name of experiment factor of interest (e.g. Genotype). One factor must be entered and there must be two or more groups per factor. Optional additional factors (e.g. Batch) can be entered using the Insert Factor button below, see Help section for more information. NOTE: Please only use letters, numbers or underscores, and the first character of each factor must be a letter">
14
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diff changeset
133 <validator type="empty_field"/>
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134 <validator type="regex" message="Please only use letters, numbers or underscores">^[\w]+$</validator>
0
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135 </param>
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136 <repeat name="rep_group" title="Group" min="2" default="2">
12
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137 <param name="groupName" type="text" label="Name" help="Name of group that the counts files belong to (e.g. WT or Mut). NOTE: Please only use letters, numbers or underscores (case sensitive), and the first character of each group must be a letter">
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
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138 <sanitizer>
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139 <valid initial="string.letters,string.digits">
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140 <add value="_"/>
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141 </valid>
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142 </sanitizer>
0
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143 </param>
3
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144 <param name="countsFile" type="data" format="tabular" multiple="true" label="Counts files"/>
0
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145 </repeat>
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146 </repeat>
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147 </when>
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148 <when value="matrix">
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149 <param name="counts" type="data" format="tabular" label="Count Matrix"/>
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150 <conditional name="fact">
12
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151 <param name="ffile" type="select" label="Input factor information from file?" help="You can choose to input the factor and group information for the samples from a file or manually enter below. NOTE: Please only use letters, numbers or underscores (case sensitive), and the first character of each sample, factor and group must be a letter">
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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152 <option value="no">No</option>
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153 <option value="yes">Yes</option>
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154 </param>
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155 <when value="yes">
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156 <param name="finfo" type="data" format="tabular" label="Factor File"/>
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157 </when>
12
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158 <when value="no">
0
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159 <repeat name="rep_factor" title="Factor" min="1">
12
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160 <param name="factorName" type="text" label="Factor Name" help="Name of experiment factor of interest (e.g. Genotype). One factor must be entered and there must be two or more groups per factor. Additional factors (e.g. Batch) can be entered using the Insert Factor button below, see Help section below. NOTE: Please only use letters, numbers or underscores, and the first character of each factor must be a letter">
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
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161 <validator type="empty_field"/>
0
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162 <validator type="regex" message="Please only use letters, numbers or underscores">^[\w]+$</validator>
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163 </param>
12
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164 <param name="groupNames" type="text" label="Groups" help="Enter the group names for the samples separated with commas e.g. WT,WT,WT,Mut,Mut,Mut. The order of the names must match the order of the samples in the columns of the count matrix. NOTE: Please only use letters, numbers or underscores (case sensitive), and the first character of each group must be a letter">
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
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diff changeset
165 <validator type="empty_field"/>
0
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166 <validator type="regex" message="Please only use letters, numbers or underscores, and separate levels by commas">^[\w,]+$</validator>
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167 </param>
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168 </repeat>
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169 </when>
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170 </conditional>
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171 </when>
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172 </conditional>
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173 <!-- Gene Annotations -->
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174 <conditional name="anno">
12
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175 <param name="annoOpt" type="select" label="Use Gene Annotations?" help="If you provide an annotation file, annotations will be added to the table(s) of differential expression results to provide descriptions for each gene. See Help section below.">
0
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176 <option value="no">No</option>
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177 <option value="yes">Yes</option>
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178 </param>
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179 <when value="yes">
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180 <param name="geneanno" type="data" format="tabular" label="Gene Annotations"/>
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181 </when>
12
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182 <when value="no"/>
0
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183 </conditional>
14
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184 <!-- Optional formula -->
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185 <param name="formula" type="text" optional="true" label="Formula for linear model" help="An optional formula for the EdgeR linear model, this will override the use of the fields in factors as a simple sum. The formula can only use elements available in the factors file. This needs to be exactly as EdgeR expect the formula, ie. `~ 0 + factor_A + factor_B:factor_C`. See EdgeR documentation for more details.">
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186 <sanitizer invalid_char="">
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187 <valid initial="string.letters,string.digits">
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188 <add value="_"/>
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189 <add value="-"/>
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190 <add value="+"/>
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191 <add value="*"/>
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192 <add value="/"/>
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193 <add value="^"/>
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194 <add value=":"/>
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195 <add value="."/>
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196 <add value="~"/>
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197 <add value=" "/>
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198 <add value="("/>
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199 <add value=")"/>
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200 <add value="@"/>
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201 <add value="$"/>
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202 </valid>
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203 </sanitizer>
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204 </param>
0
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205 <!-- Contrasts -->
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206 <conditional name="contrasts">
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207 <param name="contrastOpt" type="select" label="Input contrasts manually or through a file">
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208 <option value="manual">manually</option>
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209 <option value="file">file</option>
0
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210 </param>
14
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211 <when value="manual">
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212 <repeat name="rep_contrast" title="Contrast" min="1" default="1">
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213 <param name="contrast" type="text" label="Contrast of Interest" help="Names of two groups to compare separated by a hyphen e.g. Mut-WT. If the order is Mut-WT the fold changes in the results will be up/down in Mut relative to WT. If you have more than one contrast enter each separately using the Insert Contrast button below. For differences between contrasts use e.g. (MT.t1-MT.t0)-(WT.t1-WT.t0). For more info, see Chapter 8 in the limma User's guide: https://www.bioconductor.org/packages/release/bioc/vignettes/limma/inst/doc/usersguide.pdf or https://bioconductor.org/packages/release/bioc/vignettes/edgeR/inst/doc/edgeRUsersGuide.pdf page 36 for nested comparisons.">
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214 <validator type="empty_field"/>
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215 <validator type="regex" message="Please only use letters, numbers, parentheses, colon or underscores">^[\w\-():]+$</validator>
14
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216 </param>
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217 </repeat>
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218 </when>
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219 <when value="file">
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220 <param name="cinfo" optional="true" type="data" format="tabular" label="Contrasts File" help="Setting this file will ignore any manually added contrasts above, make sure to remove any contrast fields above pressing the trash bin icon, or the tool will fail. First line of the file must be a header, below that each separate contrast should be on a line. Contrast formulas need to be based on ther factors data and potentially the formula provided. See EdgeR documentation on contrasts for more details."/>
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221 </when>
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222 </conditional>
0
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223 <!-- Filter Options -->
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224 <section name="f" expanded="false" title="Filter Low Counts">
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225 <conditional name="filt">
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226 <param name="filt_select" type="select" label="Filter lowly expressed genes?" help="Treat genes with very low expression as unexpressed and filter out. See the Filter Low Counts section below for more information. Default: No">
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227 <option value="no" selected="true">No</option>
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228 <option value="yes">Yes</option>
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229 </param>
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230 <when value="yes">
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231 <conditional name="cformat">
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232 <param name="format_select" type="select" label="Filter on CPM or Count values?" help="It is slightly better to base the filtering on count-per-million (CPM) rather than the raw count values so as to avoid favoring genes expressed in samples sequenced to a higher depth. ">
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233 <option value="cpm">CPM</option>
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234 <option value="counts">Counts</option>
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235 </param>
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236 <when value="cpm">
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237 <param name="cpmReq" type="float" value="1" min="0" label="Minimum CPM" help="Treat genes with CPM below this value as unexpressed and filter out. See the Filter Low Counts section below for more information."/>
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238 <param name="cpmSampleReq" type="integer" value="0" min="0" label="Minimum Samples" help="Filter out all genes that do not meet the Minimum CPM in at least this many samples. See the Filter Low Counts section below for more information."/>
0
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239 </when>
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240 <when value="counts">
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241 <param name="cntReq" type="integer" value="0" min="0" label="Minimum Count" help="Filter out all genes that do not meet this minimum count. You can choose below to apply this filter to the total count for all samples or specify the number of samples under Minimum Samples. See the Filter Low Counts section below for more information."/>
0
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242 <conditional name="samples">
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243 <param name="count_select" type="select" label="Filter on Total Count or per Sample Count values?">
0
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244 <option value="total">Total</option>
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245 <option value="sample">Sample</option>
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246 </param>
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acc76f69e8cc "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 38cf93fd693c810b4150c5d8aa3763aebc988b07"
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247 <when value="total"/>
0
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248 <when value="sample">
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249 <param name="cntSampleReq" type="integer" value="0" min="0" label="Minimum Samples" help="Filter out all genes that do not meet the Minimum Count in at least this many samples. See the Filter Low Counts section below for more information."/>
0
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250 </when>
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251 </conditional>
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252 </when>
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253 </conditional>
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254 </when>
12
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255 <when value="no"/>
0
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256 </conditional>
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257 </section>
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258 <!-- Output Options -->
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259 <section name="out" expanded="false" title="Output Options">
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260 <param name="normCounts" type="boolean" truevalue="1" falsevalue="0" checked="false" label="Output Normalised Counts Table?" help="Output a file containing the normalised counts, these are in log2 counts per million (logCPM). Default: No">
0
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261 </param>
2
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262 <param name="rscript" type="boolean" truevalue="True" falsevalue="False" checked="False" label="Output Rscript?" help="If this option is set to Yes, the Rscript used will be provided as a text file in the output. Default: No"/>
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263 <param name="rdaOption" type="boolean" truevalue="1" falsevalue="0" checked="false" label="Output RData file?" help="Output all the data used by R to construct the plots and tables, can be loaded into R. A link to the RData file will be provided in the HTML report. Default: No">
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264 </param>
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265 </section>
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266 <!-- Advanced Options -->
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267 <section name="adv" expanded="false" title="Advanced Options">
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268 <param name="lfc" type="float" value="0" min="0" label="Minimum Log2 Fold Change" help="Genes above this threshold and below the p-value threshold are considered significant and highlighted in the MD plot. Default: 0."/>
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269 <param name="pVal" type="float" value="0.05" min="0" max="1" label="P-Value Adjusted Threshold" help="Genes below this threshold are considered significant and highlighted in the MD plot. If either BH(1995) or BY(2001) are selected then this value is a false-discovery-rate control. If Holm(1979) is selected then this is an adjusted p-value for family-wise error rate. Default: 0.05."/>
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270 <param name="pAdjust" type="select" label="P-Value Adjustment Method" help="Default: BH">
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271 <option value="BH" selected="true">Benjamini and Hochberg (1995)</option>
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272 <option value="BY">Benjamini and Yekutieli (2001)</option>
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273 <option value="holm">Holm (1979)</option>
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274 <option value="none">None</option>
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275 </param>
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276 <param name="normalisationOption" type="select" label="Normalisation Method" help="Default: TMM">
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277 <option value="TMM" selected="true">TMM</option>
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278 <option value="RLE">RLE</option>
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279 <option value="upperquartile">Upperquartile</option>
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280 <option value="none">None (Don't normalise)</option>
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281 </param>
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282 <param name="robOption" type="boolean" truevalue="1" falsevalue="0" checked="true" label="Use Robust Settings?" help="Using robust settings is usually recommended to protect against outlier genes. Default: Yes"/>
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283 <param name="lrtOption" type="boolean" truevalue="1" falsevalue="0" checked="false" label="Use Likelihood Ratio Test?" help="Use likelihood ratio test instead of quasi-likelihood F-test. Default: No"/>
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284 </section>
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285 </inputs>
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286 <outputs>
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287 <data name="outReport" format="html" label="${tool.name} on ${on_string}: Report"/>
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288 <collection name="outTables" type="list" label="${tool.name} on ${on_string}: Tables">
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289 <discover_datasets pattern="(?P&lt;name&gt;.+)\.tsv$" format="tabular" directory="output_dir" visible="false"/>
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290 </collection>
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291 <data name="rscript" format="txt" label="${tool.name} on ${on_string}: Rscript">
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292 <filter>out['rscript']</filter>
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293 </data>
0
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294 </outputs>
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295 <tests>
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296 <!-- Ensure report is output -->
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297 <test expect_num_outputs="2">
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298 <param name="input|format" value="matrix"/>
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299 <param name="input|counts" value="matrix.txt"/>
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300 <param name="input|fact|ffile" value="no"/>
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301 <repeat name="input|fact|rep_factor">
0
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302 <param name="factorName" value="Genotype"/>
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303 <param name="groupNames" value="Mut,Mut,Mut,WT,WT,WT"/>
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304 </repeat>
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305 <param name="contrasts|contrastOpt" value="manual"/>
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306 <repeat name="contrasts|rep_contrast">
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307 <param name="contrast" value="Mut-WT"/>
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308 </repeat>
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309 <repeat name="contrasts|rep_contrast">
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310 <param name="contrast" value="WT-Mut"/>
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311 </repeat>
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312 <param name="adv|normalisationOption" value="TMM"/>
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313 <output_collection name="outTables" count="2">
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314 <element name="edgeR_Mut-WT" ftype="tabular">
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315 <assert_contents>
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316 <has_text_matching expression="GeneID.*logFC.*logCPM.*F.*PValue.*FDR"/>
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317 <has_text_matching expression="11304.*0.4582"/>
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318 </assert_contents>
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319 </element>
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320 <element name="edgeR_WT-Mut" ftype="tabular">
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321 <assert_contents>
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322 <has_text_matching expression="GeneID.*logFC.*logCPM.*F.*PValue.*FDR"/>
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323 <has_text_matching expression="11304.*-0.4582"/>
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324 </assert_contents>
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325 </element>
0
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326 </output_collection>
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327 <output name="outReport">
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328 <assert_contents>
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329 <has_text text="edgeR Analysis Output"/>
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330 <has_text text="quasi-likelihood"/>
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331 <not_has_text text="likelihood ratio"/>
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332 <not_has_text text="RData"/>
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333 </assert_contents>
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334 </output>
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335 </test>
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336 <!-- Complex contrasts constructions -->
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337 <test expect_num_outputs="2">
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338 <param name="input|format" value="matrix"/>
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339 <param name="input|counts" value="matrix-complex.txt"/>
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340 <param name="input|fact|ffile" value="no"/>
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341 <repeat name="input|fact|rep_factor">
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342 <param name="factorName" value="Genotype"/>
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343 <param name="groupNames" value="MutA,MutA,MutA,MutB,MutB,MutB,WTA,WTA,WTA,WTB,WTB,WTB"/>
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344 </repeat>
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345 <param name="contrasts|contrastOpt" value="manual"/>
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346 <repeat name="contrasts|rep_contrast">
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347 <param name="contrast" value="(MutA-MutB)-(WTA-WTB)"/>
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348 </repeat>
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349 <param name="adv|normalisationOption" value="TMM"/>
7
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350 <output_collection name="outTables" count="1">
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351 <element name="edgeR_(MutA-MutB)-(WTA-WTB)" ftype="tabular">
7
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352 <assert_contents>
12
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353 <has_text_matching expression="GeneID.*logFC.*logCPM.*F.*PValue.*FDR"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
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354 <has_text_matching expression="11304.*15.53"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
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355 </assert_contents>
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356 </element>
7
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357 </output_collection>
12
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358 <output name="outReport">
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359 <assert_contents>
12
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360 <has_text text="edgeR Analysis Output"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
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361 <has_text text="quasi-likelihood"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
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362 <not_has_text text="likelihood ratio"/>
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iuc
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363 <not_has_text text="RData"/>
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364 </assert_contents>
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iuc
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365 </output>
47094543a5ff planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 106b877ec24a2422c0c13e37bf275c7441108b36
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366 </test>
0
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367 <!-- Ensure annotation file input works -->
12
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368 <test expect_num_outputs="2">
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369 <param name="input|format" value="matrix"/>
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370 <param name="anno|annoOpt" value="yes"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
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371 <param name="anno|geneanno" value="anno.txt"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
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372 <param name="input|counts" value="matrix.txt"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
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373 <param name="input|fact|ffile" value="no"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
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374 <repeat name="input|fact|rep_factor">
0
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375 <param name="factorName" value="Genotype"/>
12
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diff changeset
376 <param name="groupNames" value="Mut,Mut,Mut,WT,WT,WT"/>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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377 </repeat>
15
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378 <param name="contrasts|contrastOpt" value="manual"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
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379 <repeat name="contrasts|rep_contrast">
12
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380 <param name="contrast" value="Mut-WT"/>
0
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381 </repeat>
15
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382 <param name="adv|normalisationOption" value="TMM"/>
0
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383 <output_collection name="outTables" count="1">
12
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384 <element name="edgeR_Mut-WT" ftype="tabular">
2
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parents: 1
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385 <assert_contents>
12
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diff changeset
386 <has_text_matching expression="EntrezID.*Symbol.*logFC.*logCPM.*F.*PValue.*FDR"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
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387 <has_text_matching expression="11304.*Abca4.*0.4582"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
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388 </assert_contents>
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389 </element>
0
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390 </output_collection>
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391 </test>
2
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392 <!-- Ensure RScript and RData file can be output -->
12
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393 <test expect_num_outputs="3">
15
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394 <param name="input|format" value="matrix"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
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395 <param name="out|rscript" value="True"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
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396 <param name="out|rdaOption" value="true"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
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397 <param name="input|counts" value="matrix.txt"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
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398 <param name="input|fact|ffile" value="no"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
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399 <repeat name="input|fact|rep_factor">
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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400 <param name="factorName" value="Genotype"/>
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diff changeset
401 <param name="groupNames" value="Mut,Mut,Mut,WT,WT,WT"/>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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402 </repeat>
15
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iuc
parents: 14
diff changeset
403 <param name="contrasts|contrastOpt" value="manual"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
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404 <repeat name="contrasts|rep_contrast">
12
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parents: 11
diff changeset
405 <param name="contrast" value="Mut-WT"/>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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406 </repeat>
15
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diff changeset
407 <param name="adv|normalisationOption" value="TMM"/>
12
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iuc
parents: 11
diff changeset
408 <output name="outReport">
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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409 <assert_contents>
12
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iuc
parents: 11
diff changeset
410 <has_text text="RData"/>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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411 </assert_contents>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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412 </output>
4
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413 <output name="rscript">
9a62dbdb122b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 230fc767e2e402ee460440afab0e348f2ccab179
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414 <assert_contents>
12
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parents: 11
diff changeset
415 <has_text_matching expression="Task run time"/>
4
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416 </assert_contents>
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417 </output>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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418 </test>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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419 <!-- Ensure secondary factors work -->
12
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420 <test expect_num_outputs="2">
15
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parents: 14
diff changeset
421 <param name="input|format" value="matrix"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
422 <param name="input|counts" value="matrix.txt"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
423 <param name="input|fact|ffile" value="no"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
424 <repeat name="input|fact|rep_factor">
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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diff changeset
425 <param name="factorName" value="Genotype"/>
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parents: 11
diff changeset
426 <param name="groupNames" value="Mut,Mut,Mut,WT,WT,WT"/>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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diff changeset
427 </repeat>
15
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diff changeset
428 <repeat name="input|fact|rep_factor">
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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diff changeset
429 <param name="factorName" value="Batch"/>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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diff changeset
430 <param name="groupNames" value="b1,b2,b3,b1,b2,b3"/>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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431 </repeat>
15
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parents: 14
diff changeset
432 <param name="contrasts|contrastOpt" value="manual"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
433 <repeat name="contrasts|rep_contrast">
12
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parents: 11
diff changeset
434 <param name="contrast" value="Mut-WT"/>
0
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435 </repeat>
15
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diff changeset
436 <param name="adv|normalisationOption" value="TMM"/>
12
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iuc
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diff changeset
437 <output_collection name="outTables" count="1">
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
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diff changeset
438 <element name="edgeR_Mut-WT" ftype="tabular">
2
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parents: 1
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439 <assert_contents>
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diff changeset
440 <has_text_matching expression="GeneID.*logFC.*logCPM.*F.*PValue.*FDR"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
441 <has_text_matching expression="11304.*0.4584"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
442 </assert_contents>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
443 </element>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
444 </output_collection>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
445 </test>
4
9a62dbdb122b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 230fc767e2e402ee460440afab0e348f2ccab179
iuc
parents: 3
diff changeset
446 <!-- Ensure factors file with unordered samples works -->
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
447 <test expect_num_outputs="2">
15
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
448 <param name="input|format" value="matrix"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
449 <param name="input|fact|ffile" value="yes"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
450 <param name="input|fact|finfo" value="factorinfo.txt"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
451 <param name="input|counts" value="matrix.txt"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
452 <param name="contrasts|contrastOpt" value="manual"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
453 <repeat name="contrasts|rep_contrast">
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
454 <param name="contrast" value="Mut-WT"/>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
455 </repeat>
15
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
456 <param name="adv|normalisationOption" value="TMM"/>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
457 <output_collection name="outTables" count="1">
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
458 <element name="edgeR_Mut-WT" ftype="tabular">
2
3de72f1f78aa planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents: 1
diff changeset
459 <assert_contents>
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
460 <has_text_matching expression="GeneID.*logFC.*logCPM.*F.*PValue.*FDR"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
461 <has_text_matching expression="11304.*0.4584"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
462 </assert_contents>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
463 </element>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
464 </output_collection>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
465 </test>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
466 <!-- Ensure normalised counts file output works-->
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
467 <test expect_num_outputs="2">
15
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
468 <param name="input|format" value="matrix"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
469 <param name="out|normCounts" value="true"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
470 <param name="input|counts" value="matrix.txt"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
471 <param name="input|fact|ffile" value="no"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
472 <repeat name="input|fact|rep_factor">
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
473 <param name="factorName" value="Genotype"/>
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
474 <param name="groupNames" value="Mut,Mut,Mut,WT,WT,WT"/>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
475 </repeat>
15
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
476 <param name="contrasts|contrastOpt" value="manual"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
477 <repeat name="contrasts|rep_contrast">
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
478 <param name="contrast" value="Mut-WT"/>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
479 </repeat>
15
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
480 <param name="adv|normalisationOption" value="TMM"/>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
481 <output_collection name="outTables" count="2">
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
482 <element name="edgeR_Mut-WT" ftype="tabular">
2
3de72f1f78aa planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents: 1
diff changeset
483 <assert_contents>
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
484 <has_text_matching expression="GeneID.*logFC.*logCPM.*F.*PValue.*FDR"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
485 <has_text_matching expression="11304.*0.4582"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
486 </assert_contents>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
487 </element>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
488 <element name="edgeR_normcounts" ftype="tabular">
2
3de72f1f78aa planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents: 1
diff changeset
489 <assert_contents>
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
490 <has_text_matching expression="GeneID.*Mut1.*Mut2.*Mut3.*WT1.*WT2.*WT3"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
491 <has_text_matching expression="11304.*15.75"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
492 </assert_contents>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
493 </element>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
494 </output_collection>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
495 </test>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
496 <!-- Ensure likelihood ratio option works -->
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
497 <test expect_num_outputs="2">
15
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
498 <param name="input|format" value="matrix"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
499 <param name="input|counts" value="matrix.txt"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
500 <param name="input|fact|ffile" value="no"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
501 <repeat name="input|fact|rep_factor">
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
502 <param name="factorName" value="Genotype"/>
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
503 <param name="groupNames" value="Mut,Mut,Mut,WT,WT,WT"/>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
504 </repeat>
15
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
505 <param name="contrasts|contrastOpt" value="manual"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
506 <repeat name="contrasts|rep_contrast">
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
507 <param name="contrast" value="Mut-WT"/>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
508 </repeat>
15
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
509 <param name="adv|normalisationOption" value="TMM"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
510 <param name="adv|lrtOption" value="true"/>
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
511 <output name="outReport">
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
512 <assert_contents>
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
513 <has_text text="likelihood ratio"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
514 <not_has_text text="quasi-likelihood"/>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
515 </assert_contents>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
516 </output>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
517 </test>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
518 <!-- Ensure multiple counts files input works -->
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
519 <test expect_num_outputs="2">
15
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
520 <param name="input|format" value="files"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
521 <repeat name="input|rep_factor">
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
522 <param name="factorName" value="Genotype"/>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
523 <repeat name="rep_group">
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
524 <param name="groupName" value="WT"/>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
525 <param name="countsFile" value="WT1.counts,WT2.counts,WT3.counts"/>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
526 </repeat>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
527 <repeat name="rep_group">
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
528 <param name="groupName" value="Mut"/>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
529 <param name="countsFile" value="Mut1.counts,Mut2.counts,Mut3.counts"/>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
530 </repeat>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
531 </repeat>
15
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
532 <repeat name="input|rep_factor">
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
533 <param name="factorName" value="Batch"/>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
534 <repeat name="rep_group">
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
535 <param name="groupName" value="b1"/>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
536 <param name="countsFile" value="WT1.counts,Mut1.counts"/>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
537 </repeat>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
538 <repeat name="rep_group">
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
539 <param name="groupName" value="b2"/>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
540 <param name="countsFile" value="WT2.counts,Mut2.counts"/>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
541 </repeat>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
542 <repeat name="rep_group">
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
543 <param name="groupName" value="b3"/>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
544 <param name="countsFile" value="WT3.counts,Mut3.counts"/>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
545 </repeat>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
546 </repeat>
15
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
547 <param name="anno|annoOpt" value="yes"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
548 <param name="anno|geneanno" value="anno.txt"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
549 <param name="contrasts|contrastOpt" value="manual"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
550 <repeat name="contrasts|rep_contrast">
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
551 <param name="contrast" value="Mut-WT"/>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
552 </repeat>
15
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
553 <repeat name="contrasts|rep_contrast">
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
554 <param name="contrast" value="WT-Mut"/>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
555 </repeat>
15
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
556 <param name="out|normCounts" value="true"/>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
557 <output_collection name="outTables" count="3">
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
558 <element name="edgeR_Mut-WT" ftype="tabular">
2
3de72f1f78aa planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents: 1
diff changeset
559 <assert_contents>
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
560 <has_text_matching expression="EntrezID.*Symbol.*logFC.*logCPM.*F.*PValue.*FDR"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
561 <has_text_matching expression="11304.*Abca4.*0.4584"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
562 </assert_contents>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
563 </element>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
564 <element name="edgeR_WT-Mut" ftype="tabular">
2
3de72f1f78aa planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents: 1
diff changeset
565 <assert_contents>
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
566 <has_text_matching expression="logFC.*logCPM.*F.*PValue.*FDR"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
567 <has_text_matching expression="11304.*Abca4.*-0.4584"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
568 </assert_contents>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
569 </element>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
570 <element name="edgeR_normcounts" ftype="tabular">
2
3de72f1f78aa planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents: 1
diff changeset
571 <assert_contents>
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
572 <has_text_matching expression="Mut1.*Mut2.*Mut3.*WT1.*WT2.*WT3"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
573 <has_text_matching expression="11304.*Abca4.*15.75"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
574 </assert_contents>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
575 </element>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
576 </output_collection>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
577 </test>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
578 <!-- Ensure filtering on CPM in Mnimum Samples works -->
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
579 <test expect_num_outputs="2">
15
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
580 <param name="input|format" value="matrix"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
581 <param name="input|counts" value="matrix.txt"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
582 <param name="input|fact|ffile" value="no"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
583 <repeat name="input|fact|rep_factor">
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
584 <param name="factorName" value="Genotype"/>
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
585 <param name="groupNames" value="Mut,Mut,Mut,WT,WT,WT"/>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
586 </repeat>
15
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
587 <param name="contrasts|contrastOpt" value="manual"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
588 <repeat name="contrasts|rep_contrast">
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
589 <param name="contrast" value="Mut-WT"/>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
590 </repeat>
15
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
591 <param name="adv|normalisationOption" value="TMM"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
592 <param name="f|filt|filt_select" value="yes"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
593 <param name="f|filt|cformat|format_select" value="cpm"/>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
594 <!-- real cpmReq values would be a lot lower
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
595 this is just for this tiny test dataset -->
15
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
596 <param name="f|filt|cformat|cpmReq" value="1000"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
597 <param name="f|filt|cformat|cpmSampleReq" value="3"/>
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
598 <output name="outReport">
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
599 <assert_contents>
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
600 <has_text text="CPM in at least"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
601 <not_has_text text="after summing counts for all samples"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
602 <not_has_text text="counts in at least"/>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
603 </assert_contents>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
604 </output>
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
605 <output_collection name="outTables" count="1">
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
606 <element name="edgeR_Mut-WT" ftype="tabular">
2
3de72f1f78aa planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents: 1
diff changeset
607 <assert_contents>
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
608 <has_text_matching expression="GeneID.*logFC.*logCPM.*F.*PValue.*FDR"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
609 <has_text_matching expression="11304.*0.4568"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
610 <not_has_text text="-0.0682"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
611 </assert_contents>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
612 </element>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
613 </output_collection>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
614 </test>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
615 <!-- Ensure filtering on Count in Minmum Samples works -->
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
616 <test expect_num_outputs="2">
15
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
617 <param name="input|format" value="matrix"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
618 <param name="input|counts" value="matrix.txt"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
619 <param name="input|fact|ffile" value="no"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
620 <repeat name="input|fact|rep_factor">
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
621 <param name="factorName" value="Genotype"/>
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
622 <param name="groupNames" value="Mut,Mut,Mut,WT,WT,WT"/>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
623 </repeat>
15
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
624 <param name="contrasts|contrastOpt" value="manual"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
625 <repeat name="contrasts|rep_contrast">
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
626 <param name="contrast" value="Mut-WT"/>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
627 </repeat>
15
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
628 <param name="adv|normalisationOption" value="TMM"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
629 <param name="f|filt|filt_select" value="yes"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
630 <param name="f|filt|cformat|format_select" value="counts"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
631 <param name="f|filt|cformat|cntReq" value="10"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
632 <param name="f|filt|cformat|samples|count_select" value="sample"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
633 <param name="f|filt|cformat|samples|cntSampleReq" value="3"/>
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
634 <output name="outReport">
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
635 <assert_contents>
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
636 <has_text text="counts in at least"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
637 <not_has_text text="after summing counts for all samples"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
638 <not_has_text text="CPM in at least"/>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
639 </assert_contents>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
640 </output>
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
641 <output_collection name="outTables" count="1">
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
642 <element name="edgeR_Mut-WT" ftype="tabular">
2
3de72f1f78aa planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents: 1
diff changeset
643 <assert_contents>
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
644 <has_text_matching expression="GeneID.*logFC.*logCPM.*F.*PValue.*FDR"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
645 <has_text_matching expression="11304.*0.4568"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
646 <not_has_text text="-0.0682"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
647 </assert_contents>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
648 </element>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
649 </output_collection>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
650 </test>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
651 <!-- Ensure filtering on Total Count works -->
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
652 <test expect_num_outputs="2">
15
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
653 <param name="input|format" value="matrix"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
654 <param name="input|counts" value="matrix.txt"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
655 <param name="input|fact|ffile" value="no"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
656 <repeat name="input|fact|rep_factor">
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
657 <param name="factorName" value="Genotype"/>
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
658 <param name="groupNames" value="Mut,Mut,Mut,WT,WT,WT"/>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
659 </repeat>
15
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
660 <param name="contrasts|contrastOpt" value="manual"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
661 <repeat name="contrasts|rep_contrast">
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
662 <param name="contrast" value="Mut-WT"/>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
663 </repeat>
15
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
664 <param name="adv|normalisationOption" value="TMM"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
665 <param name="f|filt|filt_select" value="yes"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
666 <param name="f|filt|cformat|format_select" value="counts"/>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
667 <!-- real cntReq values would be a lot lower
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
668 this is just for this tiny test dataset -->
15
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
669 <param name="f|filt|cformat|cntReq" value="1000"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
670 <param name="f|filt|cformat|samples|count_select" value="total"/>
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
671 <output name="outReport">
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
672 <assert_contents>
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
673 <has_text text="after summing counts for all samples"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
674 <not_has_text text="counts in at least"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
675 <not_has_text text="CPM in at least"/>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
676 </assert_contents>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
677 </output>
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
678 <output_collection name="outTables" count="1">
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
679 <element name="edgeR_Mut-WT" ftype="tabular">
2
3de72f1f78aa planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents: 1
diff changeset
680 <assert_contents>
12
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
681 <has_text_matching expression="GeneID.*logFC.*logCPM.*F.*PValue.*FDR"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
682 <has_text_matching expression="11304.*0.4568"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
683 <not_has_text text="-0.0682"/>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
684 </assert_contents>
07cc368bf34f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4955aeed800ea8e45057b7e8578dc878a07f9cfc
iuc
parents: 11
diff changeset
685 </element>
0
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
686 </output_collection>
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff changeset
687 </test>
14
c5fa04118f83 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4b17306763bc8eb92c8967c7b4b57abcc514e670
iuc
parents: 13
diff changeset
688 <!-- Ensure formula and contrast file work -->
c5fa04118f83 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4b17306763bc8eb92c8967c7b4b57abcc514e670
iuc
parents: 13
diff changeset
689 <test expect_num_outputs="2">
15
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
690 <param name="input|format" value="matrix"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
691 <param name="input|counts" value="matrix.txt"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
692 <param name="input|fact|ffile" value="no"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
693 <repeat name="input|fact|rep_factor">
14
c5fa04118f83 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4b17306763bc8eb92c8967c7b4b57abcc514e670
iuc
parents: 13
diff changeset
694 <param name="factorName" value="Genotype"/>
c5fa04118f83 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4b17306763bc8eb92c8967c7b4b57abcc514e670
iuc
parents: 13
diff changeset
695 <param name="groupNames" value="Mut,Mut,Mut,WT,WT,WT"/>
c5fa04118f83 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4b17306763bc8eb92c8967c7b4b57abcc514e670
iuc
parents: 13
diff changeset
696 </repeat>
15
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
697 <repeat name="input|fact|rep_factor">
14
c5fa04118f83 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4b17306763bc8eb92c8967c7b4b57abcc514e670
iuc
parents: 13
diff changeset
698 <param name="factorName" value="Batch"/>
c5fa04118f83 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4b17306763bc8eb92c8967c7b4b57abcc514e670
iuc
parents: 13
diff changeset
699 <param name="groupNames" value="b1,b2,b3,b1,b2,b3"/>
c5fa04118f83 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4b17306763bc8eb92c8967c7b4b57abcc514e670
iuc
parents: 13
diff changeset
700 </repeat>
15
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
701 <param name="contrasts|contrastOpt" value="file"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
702 <param name="contrasts|cinfo" value="contrasts_file.txt"/>
14
c5fa04118f83 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4b17306763bc8eb92c8967c7b4b57abcc514e670
iuc
parents: 13
diff changeset
703 <param name="formula" value="~ 0 + Genotype + Batch"/>
15
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
704 <param name="adv|normalisationOption" value="TMM"/>
14
c5fa04118f83 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4b17306763bc8eb92c8967c7b4b57abcc514e670
iuc
parents: 13
diff changeset
705 <output_collection name="outTables" count="3">
c5fa04118f83 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4b17306763bc8eb92c8967c7b4b57abcc514e670
iuc
parents: 13
diff changeset
706 <element name="edgeR_Mut-WT" ftype="tabular">
c5fa04118f83 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4b17306763bc8eb92c8967c7b4b57abcc514e670
iuc
parents: 13
diff changeset
707 <assert_contents>
c5fa04118f83 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4b17306763bc8eb92c8967c7b4b57abcc514e670
iuc
parents: 13
diff changeset
708 <has_text_matching expression="GeneID.*logFC.*logCPM.*F.*PValue.*FDR"/>
c5fa04118f83 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4b17306763bc8eb92c8967c7b4b57abcc514e670
iuc
parents: 13
diff changeset
709 <has_text_matching expression="11304.*0.4584"/>
c5fa04118f83 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4b17306763bc8eb92c8967c7b4b57abcc514e670
iuc
parents: 13
diff changeset
710 </assert_contents>
c5fa04118f83 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4b17306763bc8eb92c8967c7b4b57abcc514e670
iuc
parents: 13
diff changeset
711 </element>
c5fa04118f83 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4b17306763bc8eb92c8967c7b4b57abcc514e670
iuc
parents: 13
diff changeset
712 <element name="edgeR_WT-Mut" ftype="tabular">
c5fa04118f83 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4b17306763bc8eb92c8967c7b4b57abcc514e670
iuc
parents: 13
diff changeset
713 <assert_contents>
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iuc
parents: 13
diff changeset
714 <has_text_matching expression="GeneID.*logFC.*logCPM.*F.*PValue.*FDR"/>
c5fa04118f83 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4b17306763bc8eb92c8967c7b4b57abcc514e670
iuc
parents: 13
diff changeset
715 </assert_contents>
c5fa04118f83 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4b17306763bc8eb92c8967c7b4b57abcc514e670
iuc
parents: 13
diff changeset
716 </element>
c5fa04118f83 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4b17306763bc8eb92c8967c7b4b57abcc514e670
iuc
parents: 13
diff changeset
717 <element name="edgeR_(2*Mut_3*WT)-WT" ftype="tabular">
c5fa04118f83 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4b17306763bc8eb92c8967c7b4b57abcc514e670
iuc
parents: 13
diff changeset
718 <assert_contents>
c5fa04118f83 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4b17306763bc8eb92c8967c7b4b57abcc514e670
iuc
parents: 13
diff changeset
719 <has_text_matching expression="GeneID.*logFC.*logCPM.*F.*PValue.*FDR"/>
c5fa04118f83 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4b17306763bc8eb92c8967c7b4b57abcc514e670
iuc
parents: 13
diff changeset
720 </assert_contents>
c5fa04118f83 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4b17306763bc8eb92c8967c7b4b57abcc514e670
iuc
parents: 13
diff changeset
721 </element>
c5fa04118f83 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4b17306763bc8eb92c8967c7b4b57abcc514e670
iuc
parents: 13
diff changeset
722 </output_collection>
c5fa04118f83 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 4b17306763bc8eb92c8967c7b4b57abcc514e670
iuc
parents: 13
diff changeset
723 </test>
15
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
724 <!-- Test interaction contrasts with extended dataset (tests make.names fix for interaction terms) -->
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
725 <test expect_num_outputs="2">
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
726 <param name="input|format" value="matrix"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
727 <param name="input|counts" value="matrix-extended.txt"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
728 <param name="input|fact|ffile" value="yes"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
729 <param name="input|fact|finfo" value="factorinfo-extended.txt"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
730 <param name="formula" value="~ 0 + Genotype:Batch"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
731 <param name="contrasts|contrastOpt" value="manual"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
732 <repeat name="contrasts|rep_contrast">
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
733 <param name="contrast" value="Mut:b1-WT:b1"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
734 </repeat>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
735 <repeat name="contrasts|rep_contrast">
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
736 <param name="contrast" value="Mut:b2-Mut:b3"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
737 </repeat>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
738 <param name="adv|normalisationOption" value="TMM"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
739 <output_collection name="outTables" count="2">
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
740 <element name="edgeR_Mut.b1-WT.b1" ftype="tabular">
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
741 <assert_contents>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
742 <has_text_matching expression="GeneID.*logFC.*logCPM.*F.*PValue.*FDR"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
743 <has_text_matching expression="11304"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
744 </assert_contents>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
745 </element>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
746 <element name="edgeR_Mut.b2-Mut.b3" ftype="tabular">
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
747 <assert_contents>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
748 <has_text_matching expression="GeneID.*logFC.*logCPM.*F.*PValue.*FDR"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
749 <has_text_matching expression="11304"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
750 </assert_contents>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
751 </element>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
752 </output_collection>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
753 <output name="outReport">
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
754 <assert_contents>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
755 <has_text text="edgeR Analysis Output"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
756 <has_text text="quasi-likelihood"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
757 </assert_contents>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
758 </output>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
759 </test>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
760 <!-- Test full factorial interaction model (tests make.names fix with different interaction formula) -->
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
761 <test expect_num_outputs="2">
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
762 <param name="input|format" value="matrix"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
763 <param name="input|counts" value="matrix-extended.txt"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
764 <param name="input|fact|ffile" value="yes"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
765 <param name="input|fact|finfo" value="factorinfo-extended.txt"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
766 <param name="formula" value="~ Genotype * Batch"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
767 <param name="contrasts|contrastOpt" value="manual"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
768 <repeat name="contrasts|rep_contrast">
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
769 <param name="contrast" value="WT:b2"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
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diff changeset
770 </repeat>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
771 <repeat name="contrasts|rep_contrast">
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
772 <param name="contrast" value="WT:b3-WT:b2"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
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parents: 14
diff changeset
773 </repeat>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
774 <param name="adv|normalisationOption" value="TMM"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
775 <output_collection name="outTables" count="2">
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
776 <element name="edgeR_WT.b2" ftype="tabular">
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
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parents: 14
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777 <assert_contents>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
778 <has_text_matching expression="GeneID.*logFC.*logCPM.*F.*PValue.*FDR"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
779 <has_text_matching expression="11304"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
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780 </assert_contents>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
781 </element>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
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parents: 14
diff changeset
782 <element name="edgeR_WT.b3-WT.b2" ftype="tabular">
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
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783 <assert_contents>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
784 <has_text_matching expression="GeneID.*logFC.*logCPM.*F.*PValue.*FDR"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
parents: 14
diff changeset
785 <has_text_matching expression="11304"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
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786 </assert_contents>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
iuc
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787 </element>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
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parents: 14
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788 </output_collection>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
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diff changeset
789 <output name="outReport">
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
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790 <assert_contents>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
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parents: 14
diff changeset
791 <has_text text="edgeR Analysis Output"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
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parents: 14
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792 <has_text text="quasi-likelihood"/>
119f41ec0ada planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 76bd257ee51e9d7b912f570fb9ced1084ef44212
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793 </assert_contents>
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parents: 14
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794 </output>
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795 </test>
0
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diff changeset
796 </tests>
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iuc
parents:
diff changeset
797 <help><![CDATA[
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798 .. class:: infomark
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diff changeset
799
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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800 **What it does**
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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parents:
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801
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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802 Given a counts matrix, or a set of counts files, for example from **featureCounts**, and optional information about the genes, this tool
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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803 produces plots and tables useful in the analysis of differential gene expression.
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diff changeset
804
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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805 This tool uses the `edgeR`_ quasi-likelihood pipeline (edgeR-quasi) for differential expression analysis. This statistical methodology uses negative binomial generalized linear models, but with F-tests instead of likelihood ratio tests. This method provides stricter error rate control than other negative binomial based pipelines, including the traditional edgeR pipelines or DESeq2. While the limma pipelines are recommended for large-scale datasets, because of their speed and flexibility, the edgeR-quasi pipeline gives better performance in low-count situations. For the data analyzed in this `edgeR workflow article`_ ,the edgeR-quasi, limma-voom and limma-trend pipelines are all equally suitable and give similar results.
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806
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807 .. _edgeR: http://www.bioconductor.org/packages/release/bioc/html/edgeR.html
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808 .. _edgeR workflow article: https://f1000research.com/articles/5-1438
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809
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810 -----
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811
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812 **Inputs**
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813
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814 **Counts Data:**
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815
6
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816 The counts data can either be input as separate counts files (one sample per file) or a single count matrix (one sample per column). The rows correspond to genes, and columns correspond to the counts for the samples. Values must be tab separated, with the first row containing the sample/column labels and the first column containing the row/gene labels. The sample labels must start with a letter. Gene identifiers can be of any type but must be unique and not repeated within a counts file.
0
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817
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818 Example - **Separate Count Files**:
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819
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820 ========== =======
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821 **GeneID** **WT1**
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822 ---------- -------
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823 11287 1699
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824 11298 1905
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825 11302 6
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826 11303 2099
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827 11304 356
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828 11305 2528
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829 ========== =======
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830
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831 Example - **Single Count Matrix**:
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832
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833 ========== ======= ======= ======= ======== ======== ========
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834 **GeneID** **WT1** **WT2** **WT3** **Mut1** **Mut2** **Mut3**
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835 ---------- ------- ------- ------- -------- -------- --------
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836 11287 1699 1528 1601 1463 1441 1495
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837 11298 1905 1744 1834 1345 1291 1346
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838 11302 6 8 7 5 6 5
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839 11303 2099 1974 2100 1574 1519 1654
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840 11304 356 312 337 361 397 346
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841 11305 2528 2438 2493 1762 1942 2027
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842 ========== ======= ======= ======= ======== ======== ========
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843
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844
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845 **Gene Annotations:**
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846 Optional input for gene annotations, this can contain more
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847 information about the genes than just an ID number. The annotations will
3
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848 be available in the differential expression results table and the optional normalised counts table. The file must contain a header row and have the gene IDs in the first column. The number of rows should match that of the counts files, add NA for any gene IDs with no annotation. The Galaxy tool **annotateMyIDs** can be used to obtain annotations for human, mouse, fly and zebrafish.
0
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849
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850 Example:
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851
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852 ========== ========== ===================================================
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853 **GeneID** **Symbol** **GeneName**
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854 ---------- ---------- ---------------------------------------------------
3
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855 11287 Pzp pregnancy zone protein
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856 11298 Aanat arylalkylamine N-acetyltransferase
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857 11302 Aatk apoptosis-associated tyrosine kinase
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858 11303 Abca1 ATP-binding cassette, sub-family A (ABC1), member 1
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859 11304 Abca4 ATP-binding cassette, sub-family A (ABC1), member 4
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860 11305 Abca2 ATP-binding cassette, sub-family A (ABC1), member 2
0
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861 ========== ========== ===================================================
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862
4
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863 **Factor Information:**
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864 Enter factor names and groups in the tool form, or provide a tab-separated file that has the names of the samples in the first column and one header row. The sample names must be the same as the names in the columns of the count matrix. The second column should contain the primary factor levels (e.g. WT, Mut) with optional additional columns for any secondary factors.
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865
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866 Example:
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867
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868 ========== ============ =========
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869 **Sample** **Genotype** **Batch**
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870 ---------- ------------ ---------
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871 WT1 WT b1
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872 WT2 WT b2
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873 WT3 WT b3
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874 Mut1 Mut b1
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875 Mut2 Mut b2
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876 Mut3 Mut b3
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877 ========== ============ =========
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878
6
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879 *Factor Name:* The name of the experimental factor being investigated e.g. Genotype, Treatment. One factor must be entered, the name should start with a letter and spaces must not be used. Optionally, additional factors can be included, these are variables that might influence your experiment e.g. Batch, Gender, Subject. If additional factors are entered, an additive linear model will be used.
4
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880
6
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881 *Groups:* The names of the groups for the factor. The names should start with a letter, and only contain letters, numbers and underscores, other characters such as spaces and hyphens must not be used. If entered into the tool form above, the order must be the same as the samples (to which the groups correspond) are listed in the columns of the counts matrix, with the values separated by commas.
4
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882
14
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883 **Formula:**
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884 By default the tool will construct a formula for modelling counts based on the contents of the factors files or the factors given.
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885 This can be overriden by directly providing the EdgeR formula in section named Formula.
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886
0
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887 **Contrasts of Interest:**
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888 The contrasts you wish to make between levels.
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889 A common contrast would be a simple difference between two levels: "Mut-WT"
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890 represents the difference between the mutant and wild type genotypes.
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891 Multiple contrasts must be entered separately using the Insert Contrast button, spaces must not be used.
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892
14
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893 Alternatively, you can specify a file with contrasts. The file must contain a header (it's value is irrelevant)
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894 and one contrast per line on the first column (other columns are ignored). If using this option, make sure to
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895 remove any contrast section from the manual part, or the tool will fail.
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896
15
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897 **Working with Interactions:**
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898 When you have multiple factors, you may want to test for interaction effects between them.
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899 For example, with factors Genotype (Mut, WT) and Batch (b1, b2, b3), you might want to test
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900 whether the effect of genotype differs between batches.
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901
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902 *Interaction Formula:* To include interaction terms, use the Formula field with one of these patterns:
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903
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904 - ``~ Genotype * Batch`` - Full factorial model (main effects + interactions)
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905 - ``~ Genotype + Batch + Genotype:Batch`` - Equivalent to above
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906 - ``~ 0 + Genotype:Batch`` - Cell-means model (each group combination separately)
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907
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908 The cell-means model (``~ 0 + Genotype:Batch``) is often preferred for testing specific
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909 group comparisons as it creates individual parameters for each combination (e.g., Mut.b1, WT.b1, etc.).
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910
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911 *Interaction Contrasts:* When using interactions, contrast names will contain colons that get
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912 converted to dots automatically. For example:
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913
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914 **Cell-means model contrasts** (with ``~ 0 + Genotype:Batch``):
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915 - Input contrast: ``Mut:b1-WT:b1`` (compare Mut vs WT within batch b1)
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916 - Input contrast: ``Mut:b2-Mut:b3`` (compare batch b2 vs b3 within Mut genotype)
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917 - Output file names: ``edgeR_Mut.b1-WT.b1.tsv``, ``edgeR_Mut.b2-Mut.b3.tsv``
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918
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919 **Full factorial model contrasts** (with ``~ Genotype * Batch``):
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920 - Input contrast: ``WT:b2`` (interaction effect: WT×b2 vs baseline)
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921 - Input contrast: ``WT:b3-WT:b2`` (compare interaction effects: WT×b3 vs WT×b2)
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922 - Output file names: ``edgeR_WT.b2.tsv``, ``edgeR_WT.b3-WT.b2.tsv``
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923
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924 *Using Contrasts Files with Interactions:* When using a contrasts file instead of manual entry,
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925 the same interaction contrast syntax applies. Create a tab-separated file with a header and
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926 one contrast per line:
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927
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928 Example contrasts file for cell-means model::
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929
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930 Contrast
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931 Mut:b1-WT:b1
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932 Mut:b2-Mut:b3
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933 WT:b1-WT:b2
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934
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935 Example contrasts file for full factorial model::
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936
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937 Contrast
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938 WT:b2
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939 WT:b3-WT:b2
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940 WT
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941
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942 The colons in contrast names will be automatically converted to dots in output file names,
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943 regardless of whether contrasts are entered manually or via file.
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944
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945 Note: Ensure you have sufficient replicates for interaction models. With n factors each having
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946 k levels, you need more than n×k samples to estimate interactions properly.
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diff changeset
947
0
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948 **Filter Low Counts:**
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949 Genes with very low counts across all libraries provide little evidence for differential expression.
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950 In the biological point of view, a gene must be expressed at some minimal level before
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951 it is likely to be translated into a protein or to be biologically important. In addition, the
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parents:
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952 pronounced discreteness of these counts interferes with some of the statistical approximations
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953 that are used later in the pipeline. These genes should be filtered out prior to further
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954 analysis.
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955 As a rule of thumb, genes are dropped if they can’t possibly be expressed in all the samples
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956 for any of the conditions. Users can set their own definition of genes being expressed. Usually
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957 a gene is required to have a count of 5-10 in a library to be considered expressed in that
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958 library. Users should also filter with count-per-million (CPM) rather than filtering on the
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959 counts directly, as the latter does not account for differences in library sizes between samples.
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960
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961 Option to ignore the genes that do not show significant levels of
1844cd48af49 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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parents:
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962 expression, this filtering is dependent on two criteria: CPM/count and number of samples. You can specify to filter on CPM (Minimum CPM) or count (Minimum Count) values:
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963
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964 * **Minimum CPM:** This is the minimum count per million that a gene must have in at
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965 least the number of samples specified under Minimum Samples.
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966
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967 * **Minimum Count:** This is the minimum count that a gene must have. It can be combined with either Filter
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968 on Total Count or Minimum Samples.
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969
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970 * **Filter on Total Count:** This can be used with the Minimum Count filter to keep genes
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971 with a minimum total read count.
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972
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973 * **Minimum Samples:** This is the number of samples in which the Minimum CPM/Count
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974 requirement must be met in order for that gene to be kept.
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975
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976 If the Minimum Samples filter is applied, only genes that exhibit a CPM/count greater than the required amount in at least the number of samples specified will be used for analysis. Care should be taken to
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977 ensure that the sample requirement is appropriate. In the case of an experiment
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978 with two experimental groups each with two members, if there is a change from
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979 insignificant CPM/count to significant CPM/count but the sample requirement is set to 3,
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980 then this will cause that gene to fail the criteria. When in doubt simply do not
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981 filter or consult the `edgeR workflow article`_ for filtering recommendations.
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982
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983 **Advanced Options:**
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984
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985 By default error rate for multiple testing is controlled using Benjamini and
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986 Hochberg's false discovery rate control at a threshold value of 0.05. However
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987 there are options to change this to custom values.
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988
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989 * **Minimum log2-fold-change Required:**
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990 In addition to meeting the requirement for the adjusted statistic for
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991 multiple testing, the observation must have an absolute log2-fold-change
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992 greater than this threshold to be considered significant, thus highlighted
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993 in the MD plot.
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994
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995 * **Adjusted Threshold:**
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996 Set the threshold for the resulting value of the multiple testing control
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997 method. Only observations whose statistic falls below this value is
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998 considered significant, thus highlighted in the MD plot.
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999
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1000 * **P-Value Adjustment Method:**
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1001 Change the multiple testing control method, the options are BH(1995) and
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1002 BY(2001) which are both false discovery rate controls. There is also
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1003 Holm(1979) which is a method for family-wise error rate control.
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1004
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1005 **Normalisation Method:**
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1006 The most obvious technical factor that affects the read counts, other than gene expression
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1007 levels, is the sequencing depth of each RNA sample. edgeR adjusts any differential expression
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1008 analysis for varying sequencing depths as represented by differing library sizes. This is
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1009 part of the basic modeling procedure and flows automatically into fold-change or p-value
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1010 calculations. It is always present, and doesn’t require any user intervention.
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1011 The second most important technical influence on differential expression is one that is less
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1012 obvious. RNA-seq provides a measure of the relative abundance of each gene in each RNA
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1013 sample, but does not provide any measure of the total RNA output on a per-cell basis.
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1014 This commonly becomes important when a small number of genes are very highly expressed
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1015 in one sample, but not in another. The highly expressed genes can consume a substantial
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1016 proportion of the total library size, causing the remaining genes to be under-sampled in that
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1017 sample. Unless this RNA composition effect is adjusted for, the remaining genes may falsely
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1018 appear to be down-regulated in that sample . The edgeR `calcNormFactors` function normalizes for RNA composition by finding a set of scaling factors for the library sizes that minimize the log-fold changes between the samples for most genes. The default method for computing these scale factors uses a trimmed mean of M values (TMM) between each pair of samples. We call the product of the original library size and the scaling factor the *effective library size*. The effective library size replaces the original library size in all downsteam analyses. TMM is the recommended method for most RNA-Seq data where the majority (more than half) of the genes are believed not differentially expressed between any pair of the samples. You can change the normalisation method under **Advanced Options** above. For more information, see the `calcNormFactors` section in the `edgeR User's Guide`_.
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1019
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1020 **Robust Settings**
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1021 Option to use robust settings. Using robust settings (robust=TRUE) with the edgeR estimateDisp and glmQLFit functions is usually recommended to protect against outlier genes. This is turned on by default. Note that it is only used with the quasi-likelihood F test method. For more information, see the `edgeR workflow article`_.
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1022
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1023 **Test Method**
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1024 Option to use the likelihood ratio test instead of the quasi-likelihood F test. For more information, see the `edgeR workflow article`_.
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1025
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1026 .. _edgeR User's Guide: http://www.bioconductor.org/packages/release/bioc/html/edgeR.html
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1027
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1028 -----
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1029
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1030 **Outputs**
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1031
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1032 This tool outputs
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1033
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1034 * a table of differentially expressed genes for each contrast of interest
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1035 * a HTML report with plots and additional information
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1036
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1037 Optionally, under **Output Options** you can choose to output
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1038
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1039 * a normalised counts table
2
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1040 * the R script used by this tool
0
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1041 * an RData file
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1042
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1043 -----
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1044
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1045 **Citations**
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1046
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1047 Please try to cite the appropriate articles when you publish results obtained using software, as such citation is the main means by which the authors receive credit for their work. For the edgeR method itself, please cite Robinson et al., 2010, and for this tool (which was developed from the Galaxy limma-voom tool) please cite Liu et al., 2015.
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1048
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1049 ]]></help>
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1050 <citations>
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1051 <citation type="doi">10.1093/bioinformatics/btp616</citation>
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1052 <citation type="doi">10.1093/nar/gkv412</citation>
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1053 </citations>
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1054 </tool>