Mercurial > repos > iuc > crispr_studio
comparison crispr_studio.xml @ 0:ad4ae405cdc1 draft default tip
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/crispr_studio/ commit d746438ee434696f2867394eca7a93d6ec704320"
| author | iuc |
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| date | Wed, 14 Aug 2019 11:37:32 -0400 |
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| children |
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| -1:000000000000 | 0:ad4ae405cdc1 |
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| 1 <?xml version="1.0"?> | |
| 2 <tool id="crispr_studio" name="CRISPR Studio" version="1+galaxy0"> | |
| 3 <description> | |
| 4 facilitate and accelerate CRISPR array visualization from a GFF3 file generated with CRISPRDetect | |
| 5 </description> | |
| 6 <requirements> | |
| 7 <requirement type="package" version="1">crispr_studio</requirement> | |
| 8 </requirements> | |
| 9 <command detect_errors="exit_code"> | |
| 10 <![CDATA[ | |
| 11 ln -s '${input_data}' ./infile && | |
| 12 | |
| 13 #if $rerun.r == '-r': | |
| 14 ln -s '${rerun.prev_final}' infile.fasta_fasta36.spacermatch.mcl.final && | |
| 15 ln -s '${rerun.prev_svg}' infile.fasta_fasta36.spacermatch.mcl.svg && | |
| 16 #end if | |
| 17 | |
| 18 CRISPR_Studio | |
| 19 | |
| 20 #if $order.s_order == 'File': | |
| 21 -l '${order.isolate_list}' | |
| 22 #else: | |
| 23 -s '${order.s_order}' | |
| 24 #end if | |
| 25 | |
| 26 $f | |
| 27 $n | |
| 28 $gU | |
| 29 $gS | |
| 30 $rerun.r | |
| 31 | |
| 32 #if $cut_off: | |
| 33 -c '${cut_off}' | |
| 34 #end if | |
| 35 | |
| 36 -i ./infile | |
| 37 ]]></command> | |
| 38 <inputs> | |
| 39 <param name="input_data" type="data" format="gff" label="Input sequences" help="gff (GFF3) files are valid"/> | |
| 40 <conditional name="order"> | |
| 41 <param name='s_order' type="select" label="Order of Isolates" help="Determine order of isolates in figure (-s)"> | |
| 42 <option value="DistMatrix" selected="True">DistMatrix (Order extracted from a distance matrix based on the similarity of the arrays in the isolates.)</option> | |
| 43 <option value="CRISPRDetect">CRISPRDetect (Order in GFF File)</option> | |
| 44 <option value="File">By File</option> | |
| 45 </param> | |
| 46 <when value="DistMatrix"> | |
| 47 </when> | |
| 48 <when value="CRISPRDetect"> | |
| 49 </when> | |
| 50 <when value="File"> | |
| 51 <param name="isolate_list" type="data" format="txt" optional="true" label="List of Isolates to show" help="Generate the figure with the subset of isolates listed in the file"/> | |
| 52 </when> | |
| 53 </conditional> | |
| 54 <param name="n" argument="-n" truevalue="-n" falsevalue="" type="boolean" label="Show the size of the spacers over the boxed diamonds?" help="We recommend to use this option only for experimental analysis of the data"/> | |
| 55 <param argument="-f" truevalue="-f" falsevalue="" type="boolean" label="Skip the verification of the fasta file generated from the GFF file?" help="The verification is mainly based on the length of the spacer sequences. If a spacer is 1.5 time shorter or longer than the average spacer size of the dataset, a warning is raised and the script stops (Verification is ran by default)."/> | |
| 56 <param name="cut_off" type="integer" optional="true" label="Score cutoff for pairing of the spacers"/> | |
| 57 <param argument="-gU" truevalue="-gU" falsevalue="" type="boolean" label="Gray out unique?" help="The unique spacers will be grayed"/> | |
| 58 <param argument="-gS" truevalue="-gS" falsevalue="" type="boolean" label="Gray out similar?" help="The conserved spacers will be grayed"/> | |
| 59 <conditional name="rerun"> | |
| 60 <param argument="-r" type="select" label="Change spacer colour?" help="Use this option to keep the same color attributed to the spacer during a previous analysis"> | |
| 61 <option value="">No</option> | |
| 62 <option value="-r">Yes</option> | |
| 63 </param> | |
| 64 <when value="-r"> | |
| 65 <param name="prev_final" format="tabular" label="Previous spacer.mcl.final" type="data" help="Input a previous .final file you want to retain the colour from"/> | |
| 66 <param name="prev_svg" format="svg" label="Previous SVG" type="data" help="Input a previous .svg file you want to retain the colour from"/> | |
| 67 </when> | |
| 68 <when value=""> | |
| 69 </when> | |
| 70 </conditional> | |
| 71 <param name="outputs" type="select" multiple="true" display="checkboxes" label="Additional outputs"> | |
| 72 <option value="out_fasta36">Fasta36</option> | |
| 73 <option value="out_spacermatch">Spacermatch</option> | |
| 74 <option value="out_spacermatch_mcl">Spacermatch MCL</option> | |
| 75 <option value="out_spacermatch_mcl_col">Spacermatch MCL Col</option> | |
| 76 </param> | |
| 77 | |
| 78 </inputs> | |
| 79 <outputs> | |
| 80 <data name="output_fasta" format="tabular" label="${tool.name} on ${on_string}: fasta output" from_work_dir="infile.fasta"/> | |
| 81 <data name="output_fasta36" format="tabular" label="${tool.name} on ${on_string}: fasta_fast36 output" from_work_dir="infile.fasta_fasta36"> | |
| 82 <filter>outputs and 'out_fasta36' in outputs</filter> | |
| 83 </data> | |
| 84 <data name="output_spacermatch" format="tabular" label="${tool.name} on ${on_string}: spacermatch output" from_work_dir="infile.fasta_fasta36.spacermatch"> | |
| 85 <filter>outputs and 'out_spacermatch' in outputs</filter> | |
| 86 </data> | |
| 87 <data name="output_spacermatch_mcl" format="tabular" label="${tool.name} on ${on_string}: spacermatch.mcl output" from_work_dir="infile.fasta_fasta36.spacermatch.mcl"> | |
| 88 <filter>outputs and 'out_spacermatch_mcl' in outputs</filter> | |
| 89 </data> | |
| 90 <data name="output_spacermatch_mcl_col" format="tabular" label="${tool.name} on ${on_string}: spacermatch.mcl.col output" from_work_dir="infile.fasta_fasta36.spacermatch.mcl.col"> | |
| 91 <filter>outputs and 'out_spacermatch_mcl_col' in outputs</filter> | |
| 92 </data> | |
| 93 <data name="output_spacermatch_mcl_final" format="tabular" label="${tool.name} on ${on_string}: spacematch.mcl.final output" from_work_dir="infile.fasta_fasta36.spacermatch.mcl.final"/> | |
| 94 <data name="output_svg" format="svg" label="${tool.name} on ${on_string}: SVG output" from_work_dir="infile.fasta_fasta36.spacermatch.mcl.svg"/> | |
| 95 </outputs> | |
| 96 <tests> | |
| 97 <test expect_num_outputs="7"> | |
| 98 <param name="input_data" value="crispr_test.gff" ftype="gff"/> | |
| 99 <param name="outputs" value="out_fasta36,out_spacermatch,out_spacermatch_mcl_col,out_spacermatch_mcl"/> | |
| 100 <output name="output_fasta" file="crispr_test.gff.fasta"/> | |
| 101 <output name="output_fasta36"> | |
| 102 <assert_contents> | |
| 103 <has_text text="NC_010473"/> | |
| 104 </assert_contents> | |
| 105 </output> | |
| 106 <output name="output_spacermatch" file="crispr_test.gff.fasta_fasta36.spacermatch"/> | |
| 107 <output name="output_spacermatch_mcl" file="crispr_test.gff.fasta_fasta36.spacermatch.mcl"/> | |
| 108 <output name="output_spacermatch_mcl_col"> | |
| 109 <assert_contents> | |
| 110 <has_text text="NC_010473"/> | |
| 111 </assert_contents> | |
| 112 </output> | |
| 113 <output name="output_spacermatch_mcl_final"> | |
| 114 <assert_contents> | |
| 115 <has_text text="NC_010473"/> | |
| 116 </assert_contents> | |
| 117 </output> | |
| 118 <output name="output_svg"> | |
| 119 <assert_contents> | |
| 120 <has_text text="svg"/> | |
| 121 </assert_contents> | |
| 122 </output> | |
| 123 </test> | |
| 124 <test expect_num_outputs="3"> | |
| 125 <param name="input_data" value="crispr_test_adv.gff" ftype="gff"/> | |
| 126 <param name="isolate_list" value="test_isolates.txt" ftype="txt"/> | |
| 127 <param name="n" value="true"/> | |
| 128 <output name="output_fasta" file="crispr_test_adv.gff.fasta"/> | |
| 129 <output name="output_spacermatch_mcl_final"> | |
| 130 <assert_contents> | |
| 131 <has_text text="NC_010473"/> | |
| 132 </assert_contents> | |
| 133 </output> | |
| 134 <output name="output_svg"> | |
| 135 <assert_contents> | |
| 136 <has_text text="svg"/> | |
| 137 </assert_contents> | |
| 138 </output> | |
| 139 </test> | |
| 140 </tests> | |
| 141 <help><![CDATA[ | |
| 142 | |
| 143 **What it does** | |
| 144 | |
| 145 CRISPRStudio is a program developed to facilitate and accelerate CRISPR array visualization. | |
| 146 It works by first comparing spacers sequence homology in a dataset, then assigning a two-color-code | |
| 147 to each cluster of spacers and finally writing an svg file, which can be opened in graphics vector editor. | |
| 148 | |
| 149 ]]> | |
| 150 </help> | |
| 151 <citations> | |
| 152 <citation type="bibtex"> | |
| 153 @UNPUBLISHED{moineaulab2018, | |
| 154 author = {moineaulab}, | |
| 155 title = {CRISPRStudio: Program developed to facilitate and accelerate CRISPR array visualization}, | |
| 156 year = {2018}, | |
| 157 url = {https://github.com/moineaulab/CRISPRStudio}, | |
| 158 } | |
| 159 </citation> | |
| 160 </citations> | |
| 161 </tool> |
