Mercurial > repos > iuc > cnvkit_breaks
diff breaks.xml @ 3:9ff8e2b2cca1 draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cnvkit commit fc1282ec68b346988203ead860e9b9d6a47e9efb
| author | iuc |
|---|---|
| date | Sat, 01 Mar 2025 11:48:14 +0000 |
| parents | 382f61e50156 |
| children |
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--- a/breaks.xml Mon Jan 20 16:29:08 2025 +0000 +++ b/breaks.xml Sat Mar 01 11:48:14 2025 +0000 @@ -18,8 +18,8 @@ #end if ]]></command> <inputs> - <param name="input_filename_file" type="data" format="tabular" label="Bin-Level log2 Ratios/Coverages cnr file" help="The output of the CNVkit fix" /> - <param name="input_segment_file" type="data" format="tabular" label="CN segmentation calls cnn file" help="The output of the CNVkit segment" /> + <param name="input_filename_file" type="data" format="cnr" label="Bin-Level log2 Ratios/Coverages cnr file" help="The output of the CNVkit fix" /> + <param name="input_segment_file" type="data" format="cns" label="CN segmentation calls cns file" help="The output of the CNVkit segment" /> <param argument="--min-probes" optional="true" type="integer" label="Minimum propes" value="1" help="Minimum number of covered probes to label a gene" /> </inputs> <outputs> @@ -27,8 +27,8 @@ </outputs> <tests> <test expect_num_outputs="1"> - <param name="input_filename_file" ftype="tabular" value="sample.cnr" /> - <param name="input_segment_file" ftype="tabular" value="sample.cns" /> + <param name="input_filename_file" ftype="cnr" value="sample.cnr" /> + <param name="input_segment_file" ftype="cns" value="sample.cns" /> <param name="min_probes" value="1" /> <output name="gene_breaks"> <assert_contents><has_text text="chromosome"/></assert_contents> @@ -43,6 +43,41 @@ The breaks output is a text table of tab-separated values, which is amenable to further processing by scripts and standard Unix tools such as grep, sort, cut and awk. + +----- + +**Bin-level log2 ratios (.cnr)** + +Tabular file containing normalized log2 ratios for small genomic bins (divided regions of the genome). Used to detect raw copy number variations (CNVs) before segmentation. + +.. csv-table:: + :header-rows: 0 + + "chromosome","Genomic chromosome (e.g., chr1, chrX)" + "start","Start position of the bin." + "end","End position of the bin." + "gene","Gene name(s) overlapping the bin (if applicable)." + "log2","Normalized log2 ratio (sample coverage / reference coverage)." + "depth","Average read depth in the bin." + "weight","Reliability weight of the bin (higher = more reliable)." + +----- + +**Segmented log2 ratios (.cns)** + +Tabular file with smoothed, merged segments of stable copy number, derived from the .cnr file. Represents final CNV calls. + +.. csv-table:: + :header-rows: 0 + + "chromosome","start, end: Genomic coordinates of the segment" + "gene","Gene(s) overlapping the segment." + "log2","Mean log2 ratio of the segment." + "probes","Mean log2 ratio of the segment." + "depth","Average read depth." + "weight","Reliability weight." + "p_value","Statistical confidence (lower = more significant)." + ]]></help> <expand macro="citations" /> </tool>
