Mercurial > repos > iuc > bedtools
diff getfastaBed.xml @ 8:0d3aa592ce27 draft
Uploaded
| author | iuc |
|---|---|
| date | Tue, 28 Apr 2015 22:56:34 -0400 |
| parents | |
| children | a2d4c30ba2f9 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/getfastaBed.xml Tue Apr 28 22:56:34 2015 -0400 @@ -0,0 +1,75 @@ +<tool id="bedtools_getfastabed" name="GetFastaBed" version="@WRAPPER_VERSION@.0"> + <description></description> + <macros> + <import>macros.xml</import> + </macros> + <expand macro="requirements" /> + <expand macro="stdio" /> + <command> +<![CDATA[ + bedtools getfasta + $name + $tab + $strand + $split + -fi $fasta + -bed $input + -fo $output +]]> + </command> + <inputs> + <param format="bed,vcf,gff,gff3" name="input" type="data" label="BED/VCF/GFF file" /> + <param format="fasta" name="fasta" type="data" label="Fasta file" /> + <param name="name" type="boolean" checked="false" truevalue="-name" falsevalue="" + label="Use the 'name' column in the BED file for the FASTA headers in the output FASTA file" + help="(-name)" /> + <param name="tab" type="boolean" checked="false" truevalue="-tab" falsevalue="" + label="Report extract sequences in a tab-delimited format instead of in FASTA format" + help="(-tab)" /> + <param name="strand" type="boolean" checked="false" truevalue="-s" falsevalue="" + label="Force strandedness" + help="If the feature occupies the antisense strand, the sequence will be reverse complemented. (-s)" /> + <expand macro="split" /> + </inputs> + <outputs> + <data format="fasta" name="output"> + <change_format> + <when input="tab" value="-tab" format="tabular" /> + </change_format> + </data> + </outputs> + <tests> + <test> + <param name="input" value="nucBed1.bed" ftype="bed" /> + <param name="fasta" value="nucBed1.fasta" ftype="fasta" /> + <param name="tab" value="False" /> + <param name="split" value="False" /> + <output name="output" file="getfastaBed_result1.bed" ftype="fasta" /> + </test> + <test> + <param name="input" value="nucBed1.bed" ftype="bed" /> + <param name="fasta" value="nucBed1.fasta" ftype="fasta" /> + <param name="tab" value="True" /> + <param name="split" value="False" /> + <output name="output" file="getfastaBed_result2.tabular" ftype="tabular" /> + </test> + </tests> + <help> +<![CDATA[ +**What it does** + +bedtools getfasta will extract the sequence defined by the coordinates in a BED interval and create a new FASTA entry in the output file for each extracted sequence. By default, the FASTA header for each extracted sequence will be formatted as follows: “>chrom>:<start>-<end>”. + +.. image:: $PATH_TO_IMAGES/getfasta-glyph.png + +.. class:: warningmark + +1. The headers in the input FASTA file must exactly match the chromosome column in the BED file. + +2. You can use the UNIX fold command to set the line width of the FASTA output. For example, fold -w 60 will make each line of the FASTA file have at most 60 nucleotides for easy viewing. + +@REFERENCES@ +]]> + </help> + <expand macro="citations" /> +</tool>
