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comparison getfastaBed.xml @ 10:71af3ebbbb7a draft

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author iuc
date Wed, 29 Apr 2015 12:06:53 -0400
parents 0d3aa592ce27
children a2d4c30ba2f9
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9:21f1d1c5467b 10:71af3ebbbb7a
1 <tool id="bedtools_getfastabed" name="GetFastaBed" version="@WRAPPER_VERSION@.0">
2 <description></description>
3 <macros>
4 <import>macros.xml</import>
5 </macros>
6 <expand macro="requirements" />
7 <expand macro="stdio" />
8 <command>
9 <![CDATA[
10 bedtools getfasta
11 $name
12 $tab
13 $strand
14 $split
15 -fi $fasta
16 -bed $input
17 -fo $output
18 ]]>
19 </command>
20 <inputs>
21 <param format="bed,vcf,gff,gff3" name="input" type="data" label="BED/VCF/GFF file" />
22 <param format="fasta" name="fasta" type="data" label="Fasta file" />
23 <param name="name" type="boolean" checked="false" truevalue="-name" falsevalue=""
24 label="Use the 'name' column in the BED file for the FASTA headers in the output FASTA file"
25 help="(-name)" />
26 <param name="tab" type="boolean" checked="false" truevalue="-tab" falsevalue=""
27 label="Report extract sequences in a tab-delimited format instead of in FASTA format"
28 help="(-tab)" />
29 <param name="strand" type="boolean" checked="false" truevalue="-s" falsevalue=""
30 label="Force strandedness"
31 help="If the feature occupies the antisense strand, the sequence will be reverse complemented. (-s)" />
32 <expand macro="split" />
33 </inputs>
34 <outputs>
35 <data format="fasta" name="output">
36 <change_format>
37 <when input="tab" value="-tab" format="tabular" />
38 </change_format>
39 </data>
40 </outputs>
41 <tests>
42 <test>
43 <param name="input" value="nucBed1.bed" ftype="bed" />
44 <param name="fasta" value="nucBed1.fasta" ftype="fasta" />
45 <param name="tab" value="False" />
46 <param name="split" value="False" />
47 <output name="output" file="getfastaBed_result1.bed" ftype="fasta" />
48 </test>
49 <test>
50 <param name="input" value="nucBed1.bed" ftype="bed" />
51 <param name="fasta" value="nucBed1.fasta" ftype="fasta" />
52 <param name="tab" value="True" />
53 <param name="split" value="False" />
54 <output name="output" file="getfastaBed_result2.tabular" ftype="tabular" />
55 </test>
56 </tests>
57 <help>
58 <![CDATA[
59 **What it does**
60
61 bedtools getfasta will extract the sequence defined by the coordinates in a BED interval and create a new FASTA entry in the output file for each extracted sequence. By default, the FASTA header for each extracted sequence will be formatted as follows: “>chrom>:&lt;start>-&lt;end>”.
62
63 .. image:: $PATH_TO_IMAGES/getfasta-glyph.png
64
65 .. class:: warningmark
66
67 1. The headers in the input FASTA file must exactly match the chromosome column in the BED file.
68
69 2. You can use the UNIX fold command to set the line width of the FASTA output. For example, fold -w 60 will make each line of the FASTA file have at most 60 nucleotides for easy viewing.
70
71 @REFERENCES@
72 ]]>
73 </help>
74 <expand macro="citations" />
75 </tool>