Mercurial > repos > iuc > bctools_remove_spurious_events

annotate rm_spurious_events.xml @ 4:f0008ed4664b draft default tip

Find changesets by keywords (author, files, the commit message), revision number or hash, or revset expression.
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit a6a6774ae353f915a5ca34af8f281edd91e5c665
author iuc
date Fri, 04 Oct 2024 08:55:59 +0000
parents 4739be4dcf1e
children
Ignore whitespace changes - Everywhere: Within whitespace: At end of lines:
rev   line source
4
f0008ed4664b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit a6a6774ae353f915a5ca34af8f281edd91e5c665
iuc
parents: 3
diff changeset
1 <tool id="bctools_remove_spurious_events" name="Remove spurious" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@">
0
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
2 <description>crosslinking events</description>
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
3 <macros>
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
4 <import>macros.xml</import>
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
5 </macros>
2
b9e16508f80c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit 758b42538f722759e7b5414f64c9a7973cc221b4"
iuc
parents: 0
diff changeset
6 <expand macro="requirements">
b9e16508f80c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit 758b42538f722759e7b5414f64c9a7973cc221b4"
iuc
parents: 0
diff changeset
7 <requirement type="package" version="8.31">coreutils</requirement>
b9e16508f80c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit 758b42538f722759e7b5414f64c9a7973cc221b4"
iuc
parents: 0
diff changeset
8 </expand>
0
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
9 <command detect_errors="exit_code"><![CDATA[
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
10 rm_spurious_events.py
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
11 '$events'
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
12 --threshold '$threshold'
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
13 --outfile '$events_filtered'
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
14 ]]></command>
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
15 <inputs>
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
16 <param name="events" type="data" format="bed" label="BED6 file containing alignments"/>
4
f0008ed4664b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit a6a6774ae353f915a5ca34af8f281edd91e5c665
iuc
parents: 3
diff changeset
17 <param argument="--threshold" type="float" value="0.1" optional="true" label="Threshold for spurious event removal" help="Remove all crosslinking events that combine less reads than this fraction of the maximum number of reads at this position."/>
0
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
18 </inputs>
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
19 <outputs>
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
20 <data name="events_filtered" format="bed"/>
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
21 </outputs>
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
22 <tests>
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
23 <test>
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
24 <param name="events" value="merged_pcr_dupes_spurious.bed"/>
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
25 <param name="threshold" value="0.5"/>
3
4739be4dcf1e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit 446d9e101831b0c61c4a6b75d0b9bedeb1fdbdb6"
iuc
parents: 2
diff changeset
26 <output name="events_filtered" file="merged_pcr_dupes_spurious_filtered_thresh05.bed" sort="true"/>
0
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
27 </test>
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
28 </tests>
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
29 <help><![CDATA[
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
30
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
31 bctools - Remove spurious events originating from errors in unique molecular identifiers (UMIs)
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
32 ===============================================================================================
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
33
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
34 This tool compares all events sharing the same coordinates. Among each group
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
35 of events the maximum number of PCR duplicates is determined. By default, all events that
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
36 are supported by less than 10 percent of this maximum count are removed.
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
37
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
38 Input
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
39 -----
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
40
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
41 * BED6 containing crosslinking events with score field set to number of PCR duplicates
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
42
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
43 Output
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
44 ------
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
45
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
46 * BED6 with spurious crosslinking events removed, sorted by fields chrom, start, stop, strand
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
47
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
48 ]]></help>
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
49 <expand macro="citations"/>
8f93aecd73e7 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff changeset
50 </tool>