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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit 4aa44523d9df9bf1773ed724735120476d773a19
author iuc
date Thu, 06 Mar 2025 11:50:26 +0000
parents 1bf891405b87
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8eed0ebdba3d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit aca07f4e7d683d1b7d06abb63e05d4ff1b28771f
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1 <tool id="bbtools_tadpole" name="BBTools: Tadpole" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@">
8eed0ebdba3d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit aca07f4e7d683d1b7d06abb63e05d4ff1b28771f
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2 <description>Kmer-based assembler</description>
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3 <macros>
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4 <import>macros.xml</import>
8eed0ebdba3d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit aca07f4e7d683d1b7d06abb63e05d4ff1b28771f
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5 </macros>
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6 <expand macro="edam_ontology"/>
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7 <expand macro="bio.tools"/>
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8 <expand macro="requirements"/>
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9 <command detect_errors="aggressive"><![CDATA[
0
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10 #import os
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11
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12 #if str($input_type_cond.input_type) in ['single', 'pair']:
8eed0ebdba3d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit aca07f4e7d683d1b7d06abb63e05d4ff1b28771f
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13 #set read1 = $input_type_cond.read1
8eed0ebdba3d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit aca07f4e7d683d1b7d06abb63e05d4ff1b28771f
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14 ## Tadpole uses the file extension to determine the input format.
8eed0ebdba3d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit aca07f4e7d683d1b7d06abb63e05d4ff1b28771f
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15 #set ext = '.fastq'
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16 #if $read1.ext.endswith('.gz'):
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17 #set ext = $ext + '.gz'
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18 #end if
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19 #set read1_file = 'forward' + $ext
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20 ln -s '${read1}' '${read1_file}' &&
8eed0ebdba3d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit aca07f4e7d683d1b7d06abb63e05d4ff1b28771f
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21 #if str($input_type_cond.input_type) == 'pair':
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22 #set read2 = $input_type_cond.read2
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23 #set read2_file = 'reverse' + $ext
8eed0ebdba3d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit aca07f4e7d683d1b7d06abb63e05d4ff1b28771f
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24 ln -s '${read2}' '${read2_file}' &&
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25 #end if
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26 #else:
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27 #set read1 = $input_type_cond.reads_collection['forward']
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28 #set read1_identifier = re.sub('[^\s\w\-]', '_', str($read1.element_identifier))
8eed0ebdba3d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit aca07f4e7d683d1b7d06abb63e05d4ff1b28771f
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29 ## Tadpole uses the file extension to determine the input format.
8eed0ebdba3d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit aca07f4e7d683d1b7d06abb63e05d4ff1b28771f
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30 #set ext = $read1_identifier + '.fastq'
8eed0ebdba3d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit aca07f4e7d683d1b7d06abb63e05d4ff1b28771f
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31 #if $read1.ext.endswith('.gz'):
8eed0ebdba3d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit aca07f4e7d683d1b7d06abb63e05d4ff1b28771f
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32 #set ext = $ext + '.gz'
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33 #end if
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34 #set read1_file = $read1_identifier + $ext
8eed0ebdba3d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit aca07f4e7d683d1b7d06abb63e05d4ff1b28771f
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35 ln -s '${read1}' '${read1_file}' &&
8eed0ebdba3d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit aca07f4e7d683d1b7d06abb63e05d4ff1b28771f
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36 #set read2 = $input_type_cond.reads_collection['reverse']
8eed0ebdba3d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit aca07f4e7d683d1b7d06abb63e05d4ff1b28771f
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37 #set read2_identifier = re.sub('[^\s\w\-]', '_', str($read2.element_identifier))
8eed0ebdba3d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit aca07f4e7d683d1b7d06abb63e05d4ff1b28771f
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38 #set read2_file = $read2_identifier + $ext
8eed0ebdba3d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit aca07f4e7d683d1b7d06abb63e05d4ff1b28771f
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39 ln -s '${read2}' '${read2_file}' &&
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40 #end if
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41
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42 tadpole.sh
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43
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44 #### Input parameters
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45 in='${read1_file}'
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46 #if str($input_type_cond.input_type) in ['pair', 'paired']:
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47 in2='${read2_file}'
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48 #end if
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49
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50 #### Output parameters
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51 fastadump='$output_options.fastadump'
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52 mincounttodump='$output_options.mincounttodump'
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53 #if str($output_options.fastadump):
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54 dump='${outputdump}'
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55 #end if
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56 out='${output}'
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57 #if str($input_type_cond.input_type) in ['pair', 'paired'] and str($mode_options.mode) != 'contig':
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58 out2='${output2}'
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59 #end if
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60
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61 #### Processing modes
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62 #if str($mode_options.mode) == 'contig':
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63 mode=contig
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64 #elif str($mode_options.mode) == 'extend':
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65 mode=extend
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66 #elif str($mode_options.mode) == 'correct':
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67 mode=correct
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68 #end if
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69 threads=\${GALAXY_SLOTS:-4}
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70 overwrite=true
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71 ]]></command>
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72 <inputs>
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73 <expand macro="input_type_cond"/>
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74 <section name="mode_options" title="Mode options">
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75 <param name="mode" type="select" label="Select mode">
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76 <option value="contig" selected="true">Contig: make contigs from kmers.</option>
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77 <option value="extend">Extend: extend sequences to be longer.</option>
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78 <option value="correct">Correct: error correction.</option>
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79 </param>
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80 </section>
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81 <section name="output_options" title="Output options">
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82 <param argument="fastadump" type="boolean" truevalue="t" falsevalue="f" checked="true" label="Write kmers and counts as fasta."/>
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83 <param argument="mincounttodump" type="integer" value="1" label="Dump kmers with at least this depth."/>
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84 </section>
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85 </inputs>
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86 <outputs>
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87 <data name="output" format="fastqsanger" label="${tool.name} on ${on_string} (Forward)"/>
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88 <data name="output2" format="fastqsanger" label="${tool.name} on ${on_string} (Reverse)">
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89 <filter>input_type_cond['input_type'] != 'single' and mode_options['mode'] != 'contig'</filter>
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90 </data>
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91 <data name="outputdump" format="fasta" label="${tool.name} on ${on_string} (Fastadump}">
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92 <filter>output_options['fastadump']</filter>
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93 </data>
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94 </outputs>
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95 <tests>
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96 <!-- Single correction mode -->
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97 <test expect_num_outputs="2">
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98 <param name="input_type" value="single"/>
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99 <param name="read1" value="SRX7529235_SRR10859038_1.fastq.gz"/>
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100 <param name="mode" value="correct"/>
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101 <param name="fastadump" value="t"/>
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102 <output name="output" ftype="fastqsanger">
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103 <assert_contents>
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104 <has_size value="114660" delta="100"/>
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105 <has_n_lines n="800"/>
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106 </assert_contents>
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107 </output>
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108 <output name="outputdump" ftype="fasta">
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109 <assert_contents>
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110 <has_size value="1582245" delta="100"/>
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111 <has_n_lines n="90414"/>
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112 </assert_contents>
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113 </output>
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114 </test>
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115 <!-- Paired correction mode -->
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116 <test expect_num_outputs="3">
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117 <param name="input_type" value="pair"/>
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118 <param name="read1" value="SRX7529235_SRR10859038_1.fastq.gz"/>
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119 <param name="read2" value="SRX7529235_SRR10859038_2.fastq.gz"/>
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120 <param name="mode" value="correct"/>
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121 <param name="fastadump" value="t"/>
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122 <output name="output" ftype="fastqsanger">
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123 <assert_contents>
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124 <has_size value="114660" delta="100"/>
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125 <has_n_lines n="800"/>
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126 </assert_contents>
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127 </output>
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128 <output name="output2" ftype="fastqsanger">
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129 <assert_contents>
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130 <has_size value="116806" delta="100"/>
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131 <has_n_lines n="800"/>
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132 </assert_contents>
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133 </output>
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134 <output name="outputdump" ftype="fasta">
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135 <assert_contents>
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136 <has_size value="2057965" delta="100"/>
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137 <has_n_lines n="117598"/>
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138 </assert_contents>
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139 </output>
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140 </test>
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141 <!-- Paired assembly/contig mode -->
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142 <test expect_num_outputs="2">
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143 <param name="input_type" value="pair"/>
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144 <param name="read1" value="SRX7529235_SRR10859038_1.fastq.gz"/>
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145 <param name="read2" value="SRX7529235_SRR10859038_2.fastq.gz"/>
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146 <param name="mode" value="contig"/>
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147 <param name="fastadump" value="t"/>
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148 <output name="output" ftype="fastqsanger">
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149 <assert_contents>
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150 <has_size value="606" delta="100"/>
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151 <has_n_lines n="9"/>
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152 </assert_contents>
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153 </output>
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154 <output name="outputdump" ftype="fasta">
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155 <assert_contents>
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156 <has_size value="2057965" delta="100"/>
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157 <has_n_lines n="117598"/>
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158 </assert_contents>
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159 </output>
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160 </test>
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161 </tests>
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162 <help>
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163 **What it does**
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164
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165 Tadpole is a kmer-based assembler, with additional capabilities of error-correcting and extending reads. It does not do any complicated graph analysis or scaffolding, and therefore, is not particularly good for diploid organisms. However, compared to most other assemblers, it is incredibly fast, has a very low misassembly rate, and is very adept at handling extremely irregular or super high coverage distributions. It does not have any annoying side-effects of generating temp files and directories. Also, it can selectively assemble a coverage ‘band’ from a dataset (for example, just areas with a depth between 1000x and 1500x). These features make it a good choice for microbial single-cell data, viruses, organelles, and preliminary assemblies for use in binning, quality recalibration, insert-size estimation, and so forth. Tadpole has no upper limit on kmer length.
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166
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167 </help>
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168 <expand macro="citations"/>
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169 </tool>