Mercurial > repos > iuc > ampvis2_core
comparison core.xml @ 0:6c809af05ff6 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 9ed0c3078be166bd22136771f517ae91a5198ecf
| author | iuc |
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| date | Fri, 16 Aug 2024 08:51:03 +0000 |
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| -1:000000000000 | 0:6c809af05ff6 |
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| 1 <tool id="ampvis2_core" name="ampvis2 core community analysis" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@" license="MIT"> | |
| 2 <description></description> | |
| 3 <macros> | |
| 4 <import>macros.xml</import> | |
| 5 </macros> | |
| 6 <expand macro="header"/> | |
| 7 <command detect_errors="exit_code"><![CDATA[ | |
| 8 Rscript '$rscript' | |
| 9 ]]></command> | |
| 10 <configfiles> | |
| 11 <configfile name="rscript"><![CDATA[ | |
| 12 library(ampvis2, quietly = TRUE) | |
| 13 data <- readRDS("$data") | |
| 14 #set group_by_list='c("' + '", "'.join(str($group_by).split(",")) + '")' | |
| 15 plot <- amp_core( | |
| 16 data, | |
| 17 group_by = $group_by_list, | |
| 18 core_pct = $core_pct, | |
| 19 margin_plots = "$margin_plots", | |
| 20 margin_plot_values_size = $margin_plot_values_size, | |
| 21 widths = c($widths, 1), | |
| 22 heights = c(1, $heights) | |
| 23 ) | |
| 24 @OUTPUT_TOKEN@ | |
| 25 ]]></configfile> | |
| 26 </configfiles> | |
| 27 <inputs> | |
| 28 <expand macro="rds_metadata_input_macro"/> | |
| 29 <expand macro="metadata_select_discrete" argument="group_by" optional="false" multiple="true" label="Variable(s) which contain the desired grouping of samples" help="Can also be done by sample, just provide the name of the variable containing unique sample ID's"/> | |
| 30 <param argument="core_pct" type="float" value="80" min="0" max="100" label="Threshold in percent" help="Threshold for defining abundant (a.k.a. core) OTU's for each group"/> | |
| 31 <param argument="margin_plots" type="select" label="Margin plots to show" help="Margin plots display the cumulative read abundances of all OTU's per group sharing either x or y axis with the main plot"> | |
| 32 <option value="x">Only show x-axis margin plot</option> | |
| 33 <option value="y">Only show y-axis margin plot</option> | |
| 34 <option value="xy" selected="true">Show both x and y-axis margin plots</option> | |
| 35 <option value="">Don't show any margin plots</option> | |
| 36 </param> | |
| 37 <param argument="margin_plot_values_size" type="integer" value="3" min="0" label="The size of the values" help="As indicated in the margin plots on top of the bars. Set to 0 to disable."/> | |
| 38 <param argument="widths" type="integer" value="5" min="1" label="Relative width of the main and y margin plots"/> | |
| 39 <param argument="heights" type="integer" value="5" min="1" label="Relative widths of the main and x margin plots"/> | |
| 40 <expand macro="out_format_macro"/> | |
| 41 </inputs> | |
| 42 <outputs> | |
| 43 <expand macro="out_macro"/> | |
| 44 </outputs> | |
| 45 <tests> | |
| 46 <!-- defaults --> | |
| 47 <test expect_num_outputs="1"> | |
| 48 <param name="data" value="AalborgWWTPs.rds" ftype="ampvis2"/> | |
| 49 <param name="metadata_list" value="AalborgWWTPs-metadata.list"/> | |
| 50 <param name="group_by" value="Period"/> | |
| 51 <output name="plot" value="AalborgWWTPs-core.pdf" ftype="pdf" compare="sim_size"/> | |
| 52 </test> | |
| 53 <!--multiple groups + non-default widths / heights--> | |
| 54 <test expect_num_outputs="1"> | |
| 55 <param name="data" value="AalborgWWTPs.rds" ftype="ampvis2"/> | |
| 56 <param name="metadata_list" value="AalborgWWTPs-metadata.list"/> | |
| 57 <param name="group_by" value="Plant,Period"/> | |
| 58 <param name="core_pct" value="90"/> | |
| 59 <param name="widths" value="4"/> | |
| 60 <param name="heights" value="4"/> | |
| 61 <output name="plot" value="AalborgWWTPs-core-multiple.pdf" ftype="pdf" compare="sim_size"/> | |
| 62 </test> | |
| 63 </tests> | |
| 64 <help><![CDATA[ | |
| 65 What it does | |
| 66 ============ | |
| 67 | |
| 68 Core community plot to investigate how many of the most abundant OTU's comprise | |
| 69 the "core" of groups of samples, their abundances etc. | |
| 70 | |
| 71 The Galaxy tool calls the `amp_core | |
| 72 <https://kasperskytte.github.io/ampvis2/reference/amp_core.html>`_ function | |
| 73 of the ampvis2 package. | |
| 74 | |
| 75 This analysis only makes sense without aggregating OTU's to any taxonomic level, | |
| 76 or else it will be biased by taxonomy and only be done on OTU's that have been | |
| 77 classified, which is rarely all. | |
| 78 | |
| 79 | |
| 80 @HELP_RELATIVE_ABUNDANCES@ | |
| 81 | |
| 82 Input | |
| 83 ===== | |
| 84 | |
| 85 @HELP_RDS_INPUT@ | |
| 86 | |
| 87 @HELP_METADATA_LIST_INPUT@ | |
| 88 | |
| 89 Output | |
| 90 ====== | |
| 91 | |
| 92 A core community plot in the chosen output format. | |
| 93 ]]></help> | |
| 94 <expand macro="citations"/> | |
| 95 </tool> |
