Mercurial > repos > iracooke > tpp_prophets
diff interprophet.xml @ 17:b68f292b2904 draft
Uploaded
| author | simonalpha |
|---|---|
| date | Mon, 05 Jan 2015 05:37:56 -0500 |
| parents | |
| children | 84b47300a4c8 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/interprophet.xml Mon Jan 05 05:37:56 2015 -0500 @@ -0,0 +1,72 @@ +<tool id="proteomics_search_interprophet_1" name="InterProphet" version="1.0.1"> + + <requirements> + <container type="docker">simonalpha/protk:1.4.0</container> + <requirement type="package" version="1.3">protk</requirement> + <requirement type="package" version="4.6.3">trans_proteomic_pipeline</requirement> + </requirements> + + + <description>Combine Peptide Prophet results from multiple search engines</description> + + <command>interprophet.rb --galaxy + + -o interprophet_output.pep.xml + + $use_nss + + $use_nrs + + $use_nse + + $use_nsi + + $use_nsm + + --p-thresh $p_thresh + + --threads $threads + +#for $pepxml_file in $pepxml_files: + ${pepxml_file} +#end for + + </command> + + + <inputs> + + <param name="pepxml_files" multiple="true" type="data" format="peptideprophet_pepxml" label="Peptide Prophet Results" help="These files will typically be outputs from search tools that have subsequently been run through peptide prophet"/> + + <param name="use_nss" checked="true" type="boolean" label="Include NSS in Model" help="Include NSS (Number of Sibling Searches) in Statistical Model" truevalue="" falsevalue="--no-nss"/> + <param name="use_nrs" checked="true" type="boolean" label="Include NRS in Model" help="Include NRS (Number of Replicate Spectra) in Statistical Model" truevalue="" falsevalue="--no-nrs"/> + <param name="use_nse" checked="true" type="boolean" label="Include NSE in Model" help="Include NSE (Number of Sibling Experiments) in Statistical Model" truevalue="" falsevalue="--no-nse"/> + <param name="use_nsi" checked="true" type="boolean" label="Include NSI in Model" help="Include NSI (Number of Sibling Ions) in Statistical Model" truevalue="" falsevalue="--no-nsi"/> + <param name="use_nsm" checked="true" type="boolean" label="Include NSM in Model" help="Include NSM (Number of Sibling Modifications) in Statistical Model" truevalue="" falsevalue="--no-nsm"/> + + <param name="p_thresh" help="Peptides scoring less than this value are discarded" type="float" value="0.05" min="0" max="1" label="Probability Threshold"/> + + <param name="threads" type="integer" value="1" min="0" label="Threads" help="Number of threads to use"/> + + </inputs> + <outputs> + <data format="interprophet_pepxml" name="output" label="interprophet.${pepxml_files[0].display_name}" from_work_dir="interprophet_output.pep.xml"/> + </outputs> + + <help> + +**What it does** + +Takes a set of pepXML files (possibly generated using different search engines) and calculates updated identification probabilities for each peptide. The updated probabilities are based on a statistical model that combines evidence from identifications across all of the input files, spectra, modified states and charge states. + +---- + +**Citation** + +If you use this tool please read and cite the paper describing iProphet + +Shteynberg D, et al. “iProphet: Improved statistical validation of peptide identifications in shotgun proteomics.” *Molecular and Cellular Proteomics* 10, M111.007690 (2011). + + </help> + +</tool>
