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"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
author imgteam
date Tue, 17 Sep 2019 16:57:59 -0400
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1 <?xml version='1.0' encoding='UTF-8'?>
2 <tool id="imagej2_find_maxima" name="Find maxima" version="@WRAPPER_VERSION@.0">
3 <description></description>
4 <macros>
5 <import>imagej2_macros.xml</import>
6 </macros>
7 <expand macro="fiji_requirements" />
8 <command>
9 <![CDATA[
10 python $__tool_directory__/imagej2_find_maxima.py
11 --input "$input"
12 --input_datatype $input.ext
13 --scale_when_converting $scale_when_converting
14 --weighted_rgb_conversions $weighted_rgb_conversions
15 --noise_tolerance $noise_tolerance
16 --output_type $output_type
17 --exclude_edge_maxima $exclude_edge_maxima
18 --light_background $light_background
19 --jython_script $__tool_directory__/imagej2_find_maxima_jython_script.py
20 #if str( $output_type ) == "List" or str( $output_type ) == "Count":
21 --output "$results"
22 --output_datatype "tabular"
23 #else:
24 --output "$output"
25 --output_datatype $output.ext
26 #end if
27 ]]>
28 </command>
29 <inputs>
30 <param format="bmp,eps,gif,jpg,pcx,pgm,png,psd,tiff" name="input" type="data" label="Select image"/>
31 <param name="noise_tolerance" type="integer" value="10" min="0" label="Noise tolerance" help="Maxima are ignored if they do not stand out from the surroundings by more than this value."/>
32 <param name="output_type" type="select" label="Output type">
33 <option value="Single_Points" selected="True">Single Points</option>
34 <option value="Maxima_Within_Tolerance">Maxima Within Tolerance</option>
35 <option value="Segmented_Particles">Segmented Particles</option>
36 <!--
37 This version of Fiji does not produce the same resultsTable
38 as the previous version, so eliminating these options until
39 there is time to investigate
40 <option value="List">List</option>
41 <option value="Count">Count</option>
42 -->
43 </param>
44 <param name="exclude_edge_maxima" type="select" label="Exclude edge maxima?">
45 <option value="no" selected="True">No</option>
46 <option value="yes">Yes</option>
47 </param>
48 <param name="light_background" type="select" label="Light background?">
49 <option value="no" selected="True">No</option>
50 <option value="yes">Yes</option>
51 </param>
52 <param name="scale_when_converting" type="select" label="Scale when converting?" help="Applies only to 24-bit (RGB) images, ignored for other types.">
53 <option value="yes" selected="True">Yes</option>
54 <option value="no">No</option>
55 </param>
56 <param name="weighted_rgb_conversions" type="select" label="Weighted RGB conversions?" help="Applies only to 24-bit (RGB) images, ignored for other types.">
57 <option value="no" selected="True">No</option>
58 <option value="yes">Yes</option>
59 </param>
60 </inputs>
61 <outputs>
62 <data name="output" format_source="input" label="${tool.name} on ${on_string}: ${output_type}">
63 <filter>output_type != "List" and output_type != "Count"</filter>
64 </data>
65 <data name="results" format="tabular" label="${tool.name} on ${on_string}: ${output_type}">
66 <filter>output_type == "List" or output_type == "Count"</filter>
67 </data>
68 </outputs>
69 <tests>
70 <test>
71 <param name="input" value="blobs.gif" />
72 <output name="output" file="blobs_single_points.gif" compare="sim_size" />
73 </test>
74 <test>
75 <param name="input" value="blobs.gif" />
76 <param name="noise_tolerance" value="13" />
77 <param name="output_type" value="Maxima_Within_Tolerance" />
78 <output name="output" file="blobs_tolerance.gif" compare="sim_size" />
79 </test>
80 <test>
81 <param name="input" value="blobs.gif" />
82 <param name="noise_tolerance" value="16" />
83 <param name="output_type" value="Segmented_Particles" />
84 <param name="exclude_edge_maxima" value="yes" />
85 <output name="output" file="blobs_segmented.gif" compare="sim_size" />
86 </test>
87 <!--
88 This version of Fiji does not produce the same resultsTable
89 as the previous version, so eliminating these options until
90 there is time to investigate
91 <test>
92 <param name="input" value="blobs.gif" />
93 <param name="output_type" value="List" />
94 <output name="output" file="blobs_list.tabular" />
95 </test>
96 <test>
97 <param name="input" value="blobs.gif" />
98 <param name="output_type" value="Count" />
99 <output name="output" file="blobs_count.tabular" />
100 </test>
101 -->
102 </tests>
103 <help>
104
105 **What it does**
106
107 Determines the local maxima in an image and creates a binary (mask-like) image of the same size with the
108 maxima (or one segmented particle per maximum) marked. For RGB images, maxima of luminance are selected
109 with the luminance defined as the weighted or unweighted average of the colors depending on the **Scale
110 when converting** and **Weighted RGB conversions** settings.
111
112 - **Scale when converting** (RGB images only) - If "yes", the tool will scale from min--max to 0--255 when converting from 16-bit or 32-bit to 8-bit or to scale from min--max to 0--65535 when converting from 32-bit to 16-bit.
113
114 - **Weighted RGB conversions** (RGB images only) - If "yes", the formula gray = 0.299 × red + 0.587 × green + 0.114 × blue is used to convert RGB images to grayscale. If "no", the formula gray = (red + green + blue) ⁄ 3 is used.
115
116 - **Noise Tolerance** - Maxima are ignored if they do not stand out from the surroundings by more than this value (calibrated units for calibrated images). In other words, a threshold is set at the maximum value minus noise tolerance and the contiguous area around the maximum above the threshold is analyzed. For accepting a maximum, this area must not contain any point with a value higher than the maximum. Only one maximum within this area is accepted.
117
118 - **Output Type** can be:
119
120 - **Single Points** - Produces an image with one single point per maximum.
121
122 - **Maxima Within Tolerance** - Produces an image with all points within the "Noise Tolerance" for each maximum.
123
124 - **Segmented Particles** - Assumes that each maximum belongs to a particle and segments the image by a watershed algorithm applied to the values of the image.
125
126 - **List** - Produces the list x, y of maxima in the image.
127
128 - **Count** - Produces the number of maxima in the image.
129
130 - **Exclude Edge Maxima** - Excludes maxima if the area within the noise tolerance surrounding a maximum touches the edge of the image (edge of the selection does not matter).
131
132 - **Light Background** - Select "yes" if the image background is brighter than the objects you want to find.
133
134 </help>
135 <expand macro="fiji_headless_citations" />
136 </tool>