Mercurial > repos > imgteam > imagej2_find_maxima
annotate imagej2_find_maxima.py @ 0:2395ccf9ccc1 draft default tip
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
| author | imgteam | 
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| date | Tue, 17 Sep 2019 16:57:59 -0400 | 
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| rev | line source | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 1 #!/usr/bin/env python | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 2 import argparse | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 3 import os | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 4 import shutil | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 5 import subprocess | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 6 import tempfile | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 7 import imagej2_base_utils | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 8 | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 9 parser = argparse.ArgumentParser() | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 10 parser.add_argument( '--input', dest='input', help='Path to the input file' ) | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 11 parser.add_argument( '--input_datatype', dest='input_datatype', help='Datatype of the input image' ) | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 12 parser.add_argument( '--scale_when_converting', dest='scale_when_converting', help='Scale when converting RGB image' ) | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 13 parser.add_argument( '--weighted_rgb_conversions', dest='weighted_rgb_conversions', help='Weighted RGB conversions for RGB image' ) | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 14 parser.add_argument( '--noise_tolerance', dest='noise_tolerance', type=int, help='Noise tolerance' ) | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 15 parser.add_argument( '--output_type', dest='output_type', help='Output type' ) | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 16 parser.add_argument( '--exclude_edge_maxima', dest='exclude_edge_maxima', help='Exclude edge maxima' ) | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 17 parser.add_argument( '--light_background', dest='light_background', help='Light background' ) | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 18 parser.add_argument( '--jython_script', dest='jython_script', help='Path to the Jython script' ) | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 19 parser.add_argument( '--output', dest='output', help='Path to the output file' ) | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 20 parser.add_argument( '--output_datatype', dest='output_datatype', help='Datatype of the output image' ) | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 21 args = parser.parse_args() | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 22 | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 23 tmp_dir = imagej2_base_utils.get_temp_dir() | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 24 # ImageJ expects valid image file extensions, so the Galaxy .dat extension does not | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 25 # work for some features. The following creates a symlink with an appropriate file | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 26 # extension that points to the Galaxy dataset. This symlink is used by ImageJ. | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 27 tmp_input_path = imagej2_base_utils.get_input_image_path( tmp_dir, args.input, args.input_datatype ) | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 28 tmp_output_path = imagej2_base_utils.get_temporary_image_path( tmp_dir, args.output_datatype ) | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 29 | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 30 # Define command response buffers. | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 31 tmp_out = tempfile.NamedTemporaryFile().name | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 32 tmp_stdout = open( tmp_out, 'wb' ) | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 33 tmp_err = tempfile.NamedTemporaryFile().name | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 34 tmp_stderr = open( tmp_err, 'wb' ) | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 35 # Java writes a lot of stuff to stderr, so we'll specify a file for handling actual errors. | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 36 error_log = tempfile.NamedTemporaryFile( delete=False ).name | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 37 | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 38 # Build the command line. | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 39 cmd = imagej2_base_utils.get_base_command_imagej2( None, jython_script=args.jython_script ) | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 40 if cmd is None: | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 41 imagej2_base_utils.stop_err( "ImageJ not found!" ) | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 42 cmd += ' %s' % error_log | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 43 cmd += ' %s' % tmp_input_path | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 44 cmd += ' %s' % args.scale_when_converting | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 45 cmd += ' %s' % args.weighted_rgb_conversions | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 46 cmd += ' %d' % args.noise_tolerance | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 47 cmd += ' %s' % args.output_type | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 48 cmd += ' %s' % args.exclude_edge_maxima | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 49 cmd += ' %s' % args.light_background | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 50 cmd += ' %s' % tmp_output_path | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 51 cmd += ' %s' % args.output_datatype | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 52 | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 53 # Run the command. | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 54 proc = subprocess.Popen( args=cmd, stderr=tmp_stderr, stdout=tmp_stdout, shell=True ) | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 55 rc = proc.wait() | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 56 | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 57 # Handle execution errors. | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 58 if rc != 0: | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 59 error_message = imagej2_base_utils.get_stderr_exception( tmp_err, tmp_stderr, tmp_out, tmp_stdout ) | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 60 imagej2_base_utils.stop_err( error_message ) | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 61 | 
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"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 62 # Handle processing errors. | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 63 if os.path.getsize( error_log ) > 0: | 
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"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 64 error_message = open( error_log, 'r' ).read() | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 65 imagej2_base_utils.stop_err( error_message ) | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 66 | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 67 # Save the output image. | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 68 shutil.move( tmp_output_path, args.output ) | 
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2395ccf9ccc1
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 69 imagej2_base_utils.cleanup_before_exit( tmp_dir ) | 
