Mercurial > repos > imgteam > imagej2_bunwarpj_align
annotate imagej2_enhance_contrast.py @ 0:44af15655c6c draft default tip
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
| author | imgteam | 
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| date | Tue, 17 Sep 2019 16:58:33 -0400 | 
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| children | 
| rev | line source | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 1 #!/usr/bin/env python | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 2 import argparse | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 3 import os | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 4 import shutil | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 5 import subprocess | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 6 import tempfile | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 7 import imagej2_base_utils | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 8 | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 9 parser = argparse.ArgumentParser() | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 10 parser.add_argument( '--input', dest='input', help='Path to the input file' ) | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 11 parser.add_argument( '--input_datatype', dest='input_datatype', help='Datatype of the input image' ) | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 12 parser.add_argument( '--equalize_histogram', dest='equalize_histogram', help='Equalize_histogram' ) | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 13 parser.add_argument( '--saturated_pixels', dest='saturated_pixels', type=float, default=None, help='Saturated pixel pct' ) | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 14 parser.add_argument( '--normalize', dest='normalize', help='Normalize' ) | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 15 parser.add_argument( '--jython_script', dest='jython_script', help='Path to the Jython script' ) | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 16 parser.add_argument( '--output', dest='output', help='Path to the output file' ) | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 17 parser.add_argument( '--output_datatype', dest='output_datatype', help='Datatype of the output image' ) | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 18 args = parser.parse_args() | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 19 | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 20 tmp_dir = imagej2_base_utils.get_temp_dir() | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 21 # ImageJ expects valid image file extensions, so the Galaxy .dat extension does not | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 22 # work for some features. The following creates a symlink with an appropriate file | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 23 # extension that points to the Galaxy dataset. This symlink is used by ImageJ. | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 24 tmp_input_path = imagej2_base_utils.get_input_image_path( tmp_dir, args.input, args.input_datatype ) | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 25 tmp_output_path = imagej2_base_utils.get_temporary_image_path( tmp_dir, args.output_datatype ) | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 26 | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 27 # Define command response buffers. | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 28 tmp_out = tempfile.NamedTemporaryFile().name | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 29 tmp_stdout = open( tmp_out, 'wb' ) | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 30 tmp_err = tempfile.NamedTemporaryFile().name | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 31 tmp_stderr = open( tmp_err, 'wb' ) | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 32 # Java writes a lot of stuff to stderr, so we'll specify a file for handling actual errors. | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 33 error_log = tempfile.NamedTemporaryFile( delete=False ).name | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 34 | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 35 # Build the command line. | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 36 cmd = imagej2_base_utils.get_base_command_imagej2( None, jython_script=args.jython_script ) | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 37 if cmd is None: | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 38 imagej2_base_utils.stop_err( "ImageJ not found!" ) | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 39 cmd += ' %s' % error_log | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 40 cmd += ' %s' % tmp_input_path | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 41 cmd += ' %s' % args.equalize_histogram | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 42 cmd += imagej2_base_utils.handle_none_type( args.saturated_pixels ) | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 43 cmd += ' %s' % args.normalize | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 44 cmd += ' %s' % tmp_output_path | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 45 cmd += ' %s' % args.output_datatype | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 46 | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 47 # Run the command. | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 48 proc = subprocess.Popen( args=cmd, stderr=tmp_stderr, stdout=tmp_stdout, shell=True ) | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 49 rc = proc.wait() | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 50 | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 51 # Handle execution errors. | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 52 if rc != 0: | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 53 error_message = imagej2_base_utils.get_stderr_exception( tmp_err, tmp_stderr, tmp_out, tmp_stdout ) | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 54 imagej2_base_utils.stop_err( error_message ) | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 55 | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 56 # Handle processing errors. | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 57 if os.path.getsize( error_log ) > 0: | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 58 error_message = open( error_log, 'r' ).read() | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 59 imagej2_base_utils.stop_err( error_message ) | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 60 | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 61 # Save the output image. | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 62 shutil.move( tmp_output_path, args.output ) | 
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44af15655c6c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 63 imagej2_base_utils.cleanup_before_exit( tmp_dir ) | 
