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planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
author imgteam
date Sat, 09 Feb 2019 14:13:38 -0500
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055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
imgteam
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diff changeset
1 import argparse
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
imgteam
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2 import sys
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
imgteam
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3 import pandas as pd
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
imgteam
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4 import skimage.io
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
imgteam
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5 from skimage.measure import label
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
imgteam
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6 from skimage.data import checkerboard
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
imgteam
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7 import numpy as np
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
imgteam
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8 import warnings
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
imgteam
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12 def binaryimage2points(input_file):
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
imgteam
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13 # ignore warnings that arise when importing a package that was compiled against an older version of numpy than installed; https://github.com/numpy/numpy/pull/432
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
imgteam
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14 warnings.filterwarnings("ignore")
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
imgteam
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16 img_in = skimage.io.imread(input_file, plugin='tifffile')
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
imgteam
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18 #make label image
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
imgteam
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19 label = skimage.measure.label(img_in)
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
imgteam
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21 #amount of regions
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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22 amount_label = np.max(label)
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
imgteam
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24 # iterate over all regions in order to calc center of mass
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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25 center_mass = []
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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26 for i in range(1,amount_label+1):
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
imgteam
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27 #get coordinates of region
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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28 coord = np.where(label==i)
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
imgteam
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29 # be carefull with x,y coordinates
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
imgteam
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30 center_mass.append([np.mean(coord[1]),np.mean(coord[0])])
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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32 #make data frame of detections
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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33 out_dataFrame = pd.DataFrame(center_mass)
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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36 #return
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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37 return(out_dataFrame)
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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40 if __name__ == "__main__":
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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41 parser = argparse.ArgumentParser()
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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42 parser.add_argument('input_file', help='input file')
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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43 parser.add_argument('out_file', help='out file (TSV)')
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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45 args = parser.parse_args()
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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46 input_file = args.input_file
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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47 out_file = args.out_file
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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48
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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49 #TOOL
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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50 out_dataFrame = binaryimage2points(input_file)
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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51
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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52 #Print to csv file
055f88f0315a planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/binaryimage2points/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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53 out_dataFrame.to_csv(out_file, index=False, header=False, sep="\t")