# HG changeset patch
# User greg
# Date 1517856123 18000
# Node ID 06e63b5cb02539beb376012a8e9b83eb66187a34
# Parent f7563bb242fc1f21edeb5686c4e7848c5ef67a9b
Uploaded
diff -r f7563bb242fc -r 06e63b5cb025 ideas_preprocessor.xml
--- a/ideas_preprocessor.xml Thu Feb 01 11:22:27 2018 -0500
+++ b/ideas_preprocessor.xml Mon Feb 05 13:42:03 2018 -0500
@@ -35,25 +35,14 @@
#else:
--chrom_len_file '$chromInfo'
--window_size $specify_chrom_windows_cond.window_size
- #set restrict_chromosomes = $specify_chrom_windows_cond.restrict_chromosomes_cond.restrict_chromosomes
- #if str($restrict_chromosomes) == "yes":
- #set chroms = []
- #set chrom_repeat = $specify_chrom_windows_cond.restrict_chromosomes_cond.chrom_repeat
- #for $i in $chrom_repeat.chrom
- $chroms.append($i)
- #end for
- --restrict_to_chroms ",".join(chroms)
- #end if
#end if
-#if str($bychr) == "true":
- --bychr true
-#end if
---reads_per_bp $reads_per_bp
#if str($exclude_bed_input) not in ["None", ""]:
- --exclude_bed_input '$exclude_bed_input'
-#end if
-#if str($standardize_datasets) == "true":
- --standardize_datasets true
+ #set exclude_bed_inputs = list()
+ #for $i in $exclude_bed_input:
+ $exclude_bed_input.append(str($i))
+ #end for
+ #set exclude_bed_inputs = ",".join($exclude_bed_inputs)
+ --exclude_bed_input '$exclude_bed_inputs'
#end if
--output '$output'
--output_files_path '$output.files_path'
@@ -147,18 +136,6 @@
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@@ -166,13 +143,7 @@
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@@ -220,17 +191,9 @@
* **Define chromosome window positions from a bed file** - select "No" to run whole genome segmentation or select "Yes" to segment genomes within the unit of the windows defined by the bed file. This file can be in BED3, BED4 or BED5 format, but only the first three columns (chr posst posed) will be used.
* **Window size in base pairs** - Window size in base pairs if specifying manually.
- * **Restrict processing to specified chromosomes** - select "Yes" to restrict processing to specified chromosomes.
-
- * **Chromosomes** - enter a comma-separated list of chromosomes for processing.
-
* **Select bed file for defining chromosome window positions** - select a bed file for specifying the chromosome window positions.
-* **Output chromosomes in separate files** - select "Yes" to produce separate files for each chromosome, allowing you to run IDEAS on different chromosomes separately.
-* **Calculate the signal in each window using** - use the bigWigAverageOverBed utility from the UCSC genome browser to calculate the signal (i.e., the number of reads per bp) in each window.
* **Select bed file(s) containing regions to exclude** - select one or more bed files that contains regions you'd like excluded from your datasets.
-* **Standardize all datasets** - select "Yes" to standardize all datasets (e.g., reads / total_reads * 20 million) so that the signals from different cell types become comparable - your datasets can be read counts, logp-values or fold change.
-
10.1093/nar/gkw278