Mercurial > repos > greg > gregs
diff bam_to_bigwig/README.txt @ 0:bc71417f212c
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author | g2cmnty@test-web1.g2.bx.psu.edu |
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date | Fri, 17 Jun 2011 15:25:25 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/bam_to_bigwig/README.txt Fri Jun 17 15:25:25 2011 -0400 @@ -0,0 +1,18 @@ +Convert a BAM file into a BigWig coverage file. This can be used directly from +Galaxy for display at UCSC. The advantage over standard Wiggle format is that +the data is stored in a compressed format and can be retrieved by genome +region. This allows you to view regions of arbitrarily large Wiggle file data +at UCSC while avoiding the upload costs. + +The latest version of the bam_to_wiggle.py script is available at: + +https://github.com/chapmanb/bcbb/blob/master/nextgen/scripts/bam_to_wiggle.py + +Place the script in the same directory as the XML configuration file, or +provide a soft link to it. + +This requires: + +Python2, version 2.6 or better +pysam (http://code.google.com/p/pysam/) +wigToBigWig from UCSC (http://hgdownload.cse.ucsc.edu/admin/exe/)