diff bam_to_bigwig/README.txt @ 0:bc71417f212c

Uploaded
author g2cmnty@test-web1.g2.bx.psu.edu
date Fri, 17 Jun 2011 15:25:25 -0400
parents
children
line wrap: on
line diff
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/bam_to_bigwig/README.txt	Fri Jun 17 15:25:25 2011 -0400
@@ -0,0 +1,18 @@
+Convert a BAM file into a BigWig coverage file. This can be used directly from 
+Galaxy for display at UCSC. The advantage over standard Wiggle format is that 
+the data is stored in a compressed format and can be retrieved by genome
+region. This allows you to view regions of arbitrarily large Wiggle file data
+at UCSC while avoiding the upload costs.
+
+The latest version of the bam_to_wiggle.py script is available at:
+
+https://github.com/chapmanb/bcbb/blob/master/nextgen/scripts/bam_to_wiggle.py
+
+Place the script in the same directory as the XML configuration file, or
+provide a soft link to it.
+
+This requires:
+
+Python2, version 2.6 or better
+pysam (http://code.google.com/p/pysam/)
+wigToBigWig from UCSC (http://hgdownload.cse.ucsc.edu/admin/exe/)