Mercurial > repos > greg > gene_family_phylogeny_builder
diff gene_family_phylogeny_builder.xml @ 0:f4361d941aa3 draft
Uploaded
author | greg |
---|---|
date | Tue, 11 Apr 2017 11:05:24 -0400 |
parents | |
children | e5523659847a |
line wrap: on
line diff
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/gene_family_phylogeny_builder.xml Tue Apr 11 11:05:24 2017 -0400 @@ -0,0 +1,157 @@ +<tool id="plant_tribes_gene_family_phylogeny_builder" name="GeneFamilyPhylogenyBuilder" version="@WRAPPER_VERSION@.0"> + <description>builds gene family phylogenetic trees</description> + <macros> + <import>macros.xml</import> + </macros> + <expand macro="requirements_gene_family_phylogeny_builder" /> + <expand macro="stdio" /> + <command> + <![CDATA[ + #set input_format = $input_format_cond.input_format + #set tree_inference_cond = $phylogenetic_trees_cond.tree_inference_cond + #set tree_inference = $tree_inference_cond.tree_inference + #if str($tree_inference) == 'raxml': + #set rooting_order_file_cond = $tree_inference_cond.rooting_order_file_cond + #set rooting_order_file = $rooting_order_file_cond.rooting_order_file + #if str($rooting_order_file) == 'yes': + #set rooting_order = $rooting_order_file_cond.rooting_order + #end if + #set bootstrap_replicates = $tree_inference_cond.bootstrap_replicates + #end if + + python $__tool_directory__/gene_family_phylogeny_builder.py + --orthogroup_aln '$input_aln.extra_files_path' + --scaffold '$scaffold.fields.path' + --config_dir '$scaffold.fields.path' + --method $method + --tree_inference $tree_inference + #if str($tree_inference) == 'raxml': + #if str($rooting_order_file) == 'yes': + --rooting_order '$rooting_order' + ## No else block needed here because the default rooting_order + ## configuration will be used if the --rooting_order flag is missing. + #end if + --bootstrap_replicates $bootstrap_replicates + #end if + --min_orthogroup_size $min_orthogroup_size + --max_orthogroup_size $max_orthogroup_size + --num_threads \${GALAXY_SLOTS:-4} + #if str($input_format) == 'ptalign': + --alignment_type 'aln' + --sequence_type 'protein' + #else if str($input_format) == 'ptalignca': + --alignment_type 'aln' + --sequence_type $input_format_cond.sequence_type + #else if str($input_format) == 'ptalignfiltered': + --alignment_type 'filter' + --sequence_type 'protein' + #else if str($input_format) == 'ptalignfilteredca': + --alignment_type 'filter' + --sequence_type $input_format_cond.sequence_type + #else if str($input_format) == 'ptaligntrimmed': + --alignment_type 'trim' + --sequence_type 'protein' + #else if str($input_format) == 'ptaligntrimmedca': + --alignment_type 'trim' + --sequence_type $input_format_cond.sequence_type + #end if + --output '$output_pttree' + --output_dir '$output_pttree.files_path' + ]]> + </command> + <inputs> + <conditional name="input_format_cond"> + <param name="input_format" type="select" label="Select type of data"> + <option value="ptalign">Aligned gene family sequences</option> + <option value="ptalignca">Aligned gene family sequences with codon alignments</option> + <option value="ptalignfiltered">Filtered aligned gene family sequences</option> + <option value="ptalignfilteredca">Filtered aligned gene family sequences with codon alignments</option> + <option value="ptaligntrimmed">Trimmed aligned gene family sequences</option> + <option value="ptaligntrimmedca">Trimmed aligned gene family sequences with codon alignments</option> + </param> + <when value="ptalign"> + <param name="input_ptalign" format="ptalign" type="data" label="Aligned gene family sequences"> + <validator type="empty_files_path" /> + </param> + </when> + <when value="ptalignca"> + <param name="input_ptalignca" format="ptalignca" type="data" label="Aligned gene family sequences with codon alignments"> + <validator type="empty_files_path" /> + </param> + <expand macro="param_sequence_type" /> + </when> + <when value="ptalignfiltered"> + <param name="input_ptalignfiltered" format="ptalignfiltered" type="data" label="Filtered aligned gene family sequences"> + <validator type="empty_files_path" /> + </param> + </when> + <when value="ptalignfilteredca"> + <param name="input_ptalignfilteredca" format="ptalignfilteredca" type="data" label="Filtered aligned gene family sequences with codon alignments"> + <validator type="empty_files_path" /> + </param> + <expand macro="param_sequence_type" /> + </when> + <when value="ptaligntrimmed"> + <param name="input_ptaligntrimmed" format="ptaligntrimmed" type="data" label="Trimmed aligned gene family sequences"> + <validator type="empty_files_path" /> + </param> + </when> + <when value="ptaligntrimmedca"> + <param name="input_ptaligntrimmedca" format="ptaligntrimmedca" type="data" label="Trimmed aligned gene family sequences with codon alignments"> + <validator type="empty_files_path" /> + </param> + <expand macro="param_sequence_type" /> + </when> + </conditional> + <expand macro="param_scaffold" /> + <expand macro="param_method" /> + <conditional name="tree_inference_cond"> + <param name="tree_inference" type="select" label="Phylogenetic trees inference method"> + <option value="raxml" selected="true">RAxML</option> + <option value="fasttree">FastTree</option> + </param> + <when value="raxml"> + <conditional name="rooting_order_file_cond"> + <param name="rooting_order_file" type="select" label="Select rooting order configuration for rooting trees?" help="Select No for the default rooting order configuration which uses the most distant taxon present in the orthogroup"> + <option value="no" selected="true">No</option> + <option value="yes">Yes</option> + </param> + <when value="no" /> + <when value="yes"> + <param name="rooting_order" format="txt" type="data" label="Rooting order configuration for rooting trees" /> + </when> + </conditional> + <param name="bootstrap_replicates" type="integer" value="100" min="0" label="Number of replicates for rapid bootstrap analysis and search for the best-scoring ML tree" /> + </when> + <when value="fasttree" /> + </conditional> + <param name="max_orthogroup_size" type="integer" value="100" min="0" label="Maximum number of sequences in orthogroup alignments" /> + <param name="min_orthogroup_size" type="integer" value="4" min="0" label="Minimum number of sequences in orthogroup alignments" /> + </inputs> + <outputs> + <data name="output_pttree" format="pttree" label="Gene family phylogenetic trees on ${on_string}" /> + </outputs> + <tests> + <!-- Test framework does not currently support inputs whose associated files_path contains files to be analyzed. + <test> + </test> + --> + </tests> + <help> +This tool is one of the PlantTribes collection of automated modular analysis pipelines that utilize objective classifications of +complete protein sequences from sequenced plant genomes to perform comparative evolutionary studies. It performs phylogenomic +analyses by creating multiple sequence alignments and inferred maximum likelihood phylogenies for orthogroups produced by the +**GeneFamilyAligner** tool. + +----- + +**Required options** + +**Other options** + + </help> + <citations> + <expand macro="citation1" /> + <expand macro="citations2to4" /> + </citations> +</tool>