Mercurial > repos > dfornika > snippy
diff test-data/b_out_dev/snps.log @ 21:0e733df972b5 draft
planemo upload commit d65fe6718a4e9a9fa6dba28e6702335222c3e221-dirty
author | dfornika |
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date | Tue, 12 Mar 2019 17:37:29 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/b_out_dev/snps.log Tue Mar 12 17:37:29 2019 -0400 @@ -0,0 +1,261 @@ +### cd /home/dfornika/Code/tools-iuc/tools/snippy/test-data + +### /home/dfornika/miniconda3/envs/snippy-4.3.6/bin/snippy --outdir b_out_dev --ref ref.fna --mapqual 60 --mincov 2 --minfrac 0.9 --minqual 60.0 --R1 b_1.fastq --R2 b_2.fastq + +### samtools faidx reference/ref.fa + + +### bwa index reference/ref.fa + +[bwa_index] Pack FASTA... 0.00 sec +[bwa_index] Construct BWT for the packed sequence... +[bwa_index] 0.00 seconds elapse. +[bwa_index] Update BWT... 0.00 sec +[bwa_index] Pack forward-only FASTA... 0.00 sec +[bwa_index] Construct SA from BWT and Occ... 0.00 sec +[main] Version: 0.7.17-r1188 +[main] CMD: bwa index reference/ref.fa +[main] Real time: 0.009 sec; CPU: 0.003 sec + +### mkdir -p reference/genomes && cp -f reference/ref.fa reference/genomes/ref.fa + + +### ln -sf reference/ref.fa . + + +### ln -sf reference/ref.fa.fai . + + +### mkdir -p reference/ref && gzip -c reference/ref.gff > reference/ref/genes.gff.gz + + +### bwa mem -Y -M -R '@RG\tID:b_out_dev\tSM:b_out_dev' -t 8 reference/ref.fa /home/dfornika/Code/tools-iuc/tools/snippy/test-data/b_1.fastq /home/dfornika/Code/tools-iuc/tools/snippy/test-data/b_2.fastq | samclip --max 10 --ref reference/ref.fa.fai | samtools sort -n -l 0 -T /tmp/snippy.9396. --threads 8 -m 1000M | samtools fixmate -m - - | samtools sort -l 0 -T /tmp/snippy.9396. --threads 8 -m 1000M | samtools markdup -T /tmp/snippy.9396. -r -s - - > snps.bam + +READ 10 WRITTEN 10 +EXCLUDED 0 EXAMINED 10 +PAIRED 10 SINGLE 0 +DULPICATE PAIR 0 DUPLICATE SINGLE 0 +DUPLICATE TOTAL 0 + +### samtools index snps.bam + + +### fasta_generate_regions.py reference/ref.fa.fai 1000 > reference/ref.txt + + +### freebayes-parallel reference/ref.txt 8 -p 2 -P 0 -C 2 --min-repeat-entropy 1.5 --strict-vcf -q 13 -m 60 --min-coverage 2 -F 0.05 -f reference/ref.fa snps.bam > snps.raw.vcf + + +### bcftools view --include 'FMT/GT="1/1" && QUAL>=60.0 && FMT/DP>=2 && (FMT/AO)/(FMT/DP)>=0.9' snps.raw.vcf | vt normalize -r reference/ref.fa - | bcftools annotate --remove '^INFO/TYPE,^INFO/DP,^INFO/RO,^INFO/AO,^INFO/AB,^FORMAT/GT,^FORMAT/DP,^FORMAT/RO,^FORMAT/AO,^FORMAT/QR,^FORMAT/QA,^FORMAT/GL' > snps.filt.vcf + + +### cp snps.filt.vcf snps.vcf + + +### /home/dfornika/miniconda3/envs/snippy-4.3.6/bin/snippy-vcf_to_tab --gff reference/ref.gff --ref reference/ref.fa --vcf snps.vcf > snps.tab + +Loading reference: reference/ref.fa +Loaded 1 sequences. +Loading features: reference/ref.gff +Parsing variants: snps.vcf +Converted 1 SNPs to TAB format. + +### /home/dfornika/miniconda3/envs/snippy-4.3.6/bin/snippy-vcf_extract_subs snps.filt.vcf > snps.subs.vcf + + +### bcftools convert -Oz -o snps.vcf.gz snps.vcf + + +### bcftools index -f snps.vcf.gz + + +### bcftools consensus -f reference/ref.fa -o snps.consensus.fa snps.vcf.gz + +Note: the --sample option not given, applying all records regardless of the genotype + +### bcftools convert -Oz -o snps.subs.vcf.gz snps.subs.vcf + + +### bcftools index -f snps.subs.vcf.gz + + +### bcftools consensus -f reference/ref.fa -o snps.consensus.subs.fa snps.subs.vcf.gz + +Note: the --sample option not given, applying all records regardless of the genotype + +### rm -f snps.subs.vcf.gz snps.subs.vcf.gz.csi snps.subs.vcf.gz.tbi + +### cd /home/dfornika/Code/tools-iuc/tools/snippy/test-data + +### /home/dfornika/miniconda3/envs/snippy-4.3.6/bin/snippy --force --outdir b_out_dev --rgid b --ref ref.fna --mapqual 60 --mincov 2 --minfrac 0.9 --minqual 60.0 --R1 b_1.fastq --R2 b_2.fastq + +### samtools faidx reference/ref.fa + + +### bwa index reference/ref.fa + +[bwa_index] Pack FASTA... 0.00 sec +[bwa_index] Construct BWT for the packed sequence... +[bwa_index] 0.00 seconds elapse. +[bwa_index] Update BWT... 0.00 sec +[bwa_index] Pack forward-only FASTA... 0.00 sec +[bwa_index] Construct SA from BWT and Occ... 0.00 sec +[main] Version: 0.7.17-r1188 +[main] CMD: bwa index reference/ref.fa +[main] Real time: 0.008 sec; CPU: 0.002 sec + +### mkdir -p reference/genomes && cp -f reference/ref.fa reference/genomes/ref.fa + + +### ln -sf reference/ref.fa . + + +### ln -sf reference/ref.fa.fai . + + +### mkdir -p reference/ref && gzip -c reference/ref.gff > reference/ref/genes.gff.gz + + +### bwa mem -Y -M -R '@RG\tID:b\tSM:b' -t 8 reference/ref.fa /home/dfornika/Code/tools-iuc/tools/snippy/test-data/b_1.fastq /home/dfornika/Code/tools-iuc/tools/snippy/test-data/b_2.fastq | samclip --max 10 --ref reference/ref.fa.fai | samtools sort -n -l 0 -T /tmp/snippy.10001. --threads 8 -m 1000M | samtools fixmate -m - - | samtools sort -l 0 -T /tmp/snippy.10001. --threads 8 -m 1000M | samtools markdup -T /tmp/snippy.10001. -r -s - - > snps.bam + +READ 10 WRITTEN 10 +EXCLUDED 0 EXAMINED 10 +PAIRED 10 SINGLE 0 +DULPICATE PAIR 0 DUPLICATE SINGLE 0 +DUPLICATE TOTAL 0 + +### samtools index snps.bam + + +### fasta_generate_regions.py reference/ref.fa.fai 1000 > reference/ref.txt + + +### freebayes-parallel reference/ref.txt 8 -p 2 -P 0 -C 2 --min-repeat-entropy 1.5 --strict-vcf -q 13 -m 60 --min-coverage 2 -F 0.05 -f reference/ref.fa snps.bam > snps.raw.vcf + + +### bcftools view --include 'FMT/GT="1/1" && QUAL>=60.0 && FMT/DP>=2 && (FMT/AO)/(FMT/DP)>=0.9' snps.raw.vcf | vt normalize -r reference/ref.fa - | bcftools annotate --remove '^INFO/TYPE,^INFO/DP,^INFO/RO,^INFO/AO,^INFO/AB,^FORMAT/GT,^FORMAT/DP,^FORMAT/RO,^FORMAT/AO,^FORMAT/QR,^FORMAT/QA,^FORMAT/GL' > snps.filt.vcf + + +### cp snps.filt.vcf snps.vcf + + +### /home/dfornika/miniconda3/envs/snippy-4.3.6/bin/snippy-vcf_to_tab --gff reference/ref.gff --ref reference/ref.fa --vcf snps.vcf > snps.tab + +Loading reference: reference/ref.fa +Loaded 1 sequences. +Loading features: reference/ref.gff +Parsing variants: snps.vcf +Converted 1 SNPs to TAB format. + +### /home/dfornika/miniconda3/envs/snippy-4.3.6/bin/snippy-vcf_extract_subs snps.filt.vcf > snps.subs.vcf + + +### bcftools convert -Oz -o snps.vcf.gz snps.vcf + + +### bcftools index -f snps.vcf.gz + + +### bcftools consensus -f reference/ref.fa -o snps.consensus.fa snps.vcf.gz + +Note: the --sample option not given, applying all records regardless of the genotype + +### bcftools convert -Oz -o snps.subs.vcf.gz snps.subs.vcf + + +### bcftools index -f snps.subs.vcf.gz + + +### bcftools consensus -f reference/ref.fa -o snps.consensus.subs.fa snps.subs.vcf.gz + +Note: the --sample option not given, applying all records regardless of the genotype + +### rm -f snps.subs.vcf.gz snps.subs.vcf.gz.csi snps.subs.vcf.gz.tbi + +### cd /home/dfornika/Code/tools-iuc/tools/snippy/test-data + +### /home/dfornika/miniconda3/envs/snippy-4.3.6/bin/snippy --force --outdir b_out_dev --rgid b --ref ref.fna --mapqual 60 --mincov 2 --minfrac 0.9 --minqual 60.0 --R1 b_1.fastq --R2 b_2.fastq + +### samtools faidx reference/ref.fa + + +### bwa index reference/ref.fa + +[bwa_index] Pack FASTA... 0.00 sec +[bwa_index] Construct BWT for the packed sequence... +[bwa_index] 0.00 seconds elapse. +[bwa_index] Update BWT... 0.00 sec +[bwa_index] Pack forward-only FASTA... 0.00 sec +[bwa_index] Construct SA from BWT and Occ... 0.00 sec +[main] Version: 0.7.17-r1188 +[main] CMD: bwa index reference/ref.fa +[main] Real time: 0.009 sec; CPU: 0.003 sec + +### mkdir -p reference/genomes && cp -f reference/ref.fa reference/genomes/ref.fa + + +### ln -sf reference/ref.fa . + + +### ln -sf reference/ref.fa.fai . + + +### mkdir -p reference/ref && gzip -c reference/ref.gff > reference/ref/genes.gff.gz + + +### bwa mem -Y -M -R '@RG\tID:b\tSM:b' -t 8 reference/ref.fa /home/dfornika/Code/tools-iuc/tools/snippy/test-data/b_1.fastq /home/dfornika/Code/tools-iuc/tools/snippy/test-data/b_2.fastq | samclip --max 10 --ref reference/ref.fa.fai | samtools sort -n -l 0 -T /tmp/snippy.22109. --threads 8 -m 1000M | samtools fixmate -m - - | samtools sort -l 0 -T /tmp/snippy.22109. --threads 8 -m 1000M | samtools markdup -T /tmp/snippy.22109. -r -s - - > snps.bam + +READ 10 WRITTEN 10 +EXCLUDED 0 EXAMINED 10 +PAIRED 10 SINGLE 0 +DULPICATE PAIR 0 DUPLICATE SINGLE 0 +DUPLICATE TOTAL 0 + +### samtools index snps.bam + + +### fasta_generate_regions.py reference/ref.fa.fai 1000 > reference/ref.txt + + +### freebayes-parallel reference/ref.txt 8 -p 2 -P 0 -C 2 --min-repeat-entropy 1.5 --strict-vcf -q 13 -m 60 --min-coverage 2 -F 0.05 -f reference/ref.fa snps.bam > snps.raw.vcf + + +### bcftools view --include 'FMT/GT="1/1" && QUAL>=60.0 && FMT/DP>=2 && (FMT/AO)/(FMT/DP)>=0.9' snps.raw.vcf | vt normalize -r reference/ref.fa - | bcftools annotate --remove '^INFO/TYPE,^INFO/DP,^INFO/RO,^INFO/AO,^INFO/AB,^FORMAT/GT,^FORMAT/DP,^FORMAT/RO,^FORMAT/AO,^FORMAT/QR,^FORMAT/QA,^FORMAT/GL' > snps.filt.vcf + + +### cp snps.filt.vcf snps.vcf + + +### /home/dfornika/miniconda3/envs/snippy-4.3.6/bin/snippy-vcf_to_tab --gff reference/ref.gff --ref reference/ref.fa --vcf snps.vcf > snps.tab + +Loading reference: reference/ref.fa +Loaded 1 sequences. +Loading features: reference/ref.gff +Parsing variants: snps.vcf +Converted 1 SNPs to TAB format. + +### /home/dfornika/miniconda3/envs/snippy-4.3.6/bin/snippy-vcf_extract_subs snps.filt.vcf > snps.subs.vcf + + +### bcftools convert -Oz -o snps.vcf.gz snps.vcf + + +### bcftools index -f snps.vcf.gz + + +### bcftools consensus -f reference/ref.fa -o snps.consensus.fa snps.vcf.gz + +Note: the --sample option not given, applying all records regardless of the genotype + +### bcftools convert -Oz -o snps.subs.vcf.gz snps.subs.vcf + + +### bcftools index -f snps.subs.vcf.gz + + +### bcftools consensus -f reference/ref.fa -o snps.consensus.subs.fa snps.subs.vcf.gz + +Note: the --sample option not given, applying all records regardless of the genotype + +### rm -f snps.subs.vcf.gz snps.subs.vcf.gz.csi snps.subs.vcf.gz.tbi +