Mercurial > repos > dfornika > blast_xml_to_tabular
diff blast_xml_to_tabular.xml @ 0:efe0c7b8fb78 draft
planemo upload for repository https://github.com/dfornika/galaxy/tree/master/tools/blast_xml_to_tabular commit 006cbba6513492f5a06b573c676400a2d464520b-dirty
author | dfornika |
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date | Mon, 09 Sep 2019 17:17:09 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/blast_xml_to_tabular.xml Mon Sep 09 17:17:09 2019 -0400 @@ -0,0 +1,102 @@ +<tool id="blast_xml_to_tabular" name="BLAST XML to tabular" version="1.1.0"> + <description>Convert BLAST XML output to tabular</description> + <command detect_errors="exit_code"> + <![CDATA[ + '$__tool_directory__/blast_xml_to_tabular.py' + '${blastxml_file}' + '${tabular_file}' + '${out_format}' + ]]> + </command> + <inputs> + <param name="blastxml_file" type="data" format="blastxml" label="BLAST results as XML"/> + <param name="out_format" type="select" label="Output format"> + <option value="std" selected="True">Tabular (standard 12 columns)</option> + <option value="ext">Tabular (extended 24 columns)</option> + <option value="ext+">Tabular (extended 26 columns)</option> + </param> + </inputs> + <outputs> + <data name="tabular_file" format="tabular" label="BLAST results: data $blastxml_file.hid as tabular" /> + </outputs> + <help> + +.. class:: infomark + +**What it does** + +NCBI BLAST+ (and the older NCBI 'legacy' BLAST) can output in a range of +formats including tabular and a more detailed XML format. A complex workflow +may need both the XML and the tabular output - but running BLAST twice is +slow and wasteful. + +This tool takes the BLAST XML output and by default converts it into the +standard 12 column tabular equivalent: + +====== ========= ============================================ +Column NCBI name Description +------ --------- -------------------------------------------- + 1 qseqid Query Seq-id (ID of your sequence) + 2 sseqid Subject Seq-id (ID of the database hit) + 3 pident Percentage of identical matches + 4 length Alignment length + 5 mismatch Number of mismatches + 6 gapopen Number of gap openings + 7 qstart Start of alignment in query + 8 qend End of alignment in query + 9 sstart Start of alignment in subject (database hit) + 10 send End of alignment in subject (database hit) + 11 evalue Expectation value (E-value) + 12 bitscore Bit score +====== ========= ============================================ + +The BLAST+ tools can optionally output additional columns of information, +but this takes longer to calculate. Most (but not all) of these columns are +included by selecting the extended tabular output. The extra columns are +included *after* the standard 12 columns. This is so that you can write +workflow filtering steps that accept either the 12 or 24 column tabular +BLAST output. + +====== ============= =========================================== +Column NCBI name Description +------ ------------- ------------------------------------------- + 13 sallseqid All subject Seq-id(s), separated by a ';' + 14 score Raw score + 15 nident Number of identical matches + 16 positive Number of positive-scoring matches + 17 gaps Total number of gaps + 18 ppos Percentage of positive-scoring matches + 19 qframe Query frame + 20 sframe Subject frame + 21 qseq Aligned part of query sequence + 22 sseq Aligned part of subject sequence + 23 qlen Query sequence length + 24 slen Subject sequence length +====== ============= =========================================== + +Very slight modifications were made to the "BLAST XML to tabular" tool that +ships with Galaxy to output two more column columns: + +====== ============= =========================================== +Column NCBI name Description +------ ------------- ------------------------------------------- + 25 pcov Percentage coverage + 26 sallseqdescr All subject Seq-descr(s), separated by a ',' +====== ============= =========================================== + +---- + +.. class:: infomark + +This is a slightly modified version of a tool that ships with Galaxy. +If the 12 or 24 columns formats are desired, use the original tool. + +.. class:: warningmark + +Beware that the XML file (and thus the conversion) and the tabular output +direct from BLAST+ may differ in the presence of XXXX masking on regions +low complexity (columns 21 and 22), and thus also calculated figures like +the percentage identity (column 3). + + </help> +</tool>