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author devteam
date Tue, 01 Apr 2014 09:11:48 -0400
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1 <tool id="gatk_variant_apply_recalibration" name="Apply Variant Recalibration" version="0.0.4">
2 <description></description>
3 <requirements>
4 <requirement type="package" version="1.4">gatk</requirement>
5 </requirements>
6 <macros>
7 <import>gatk_macros.xml</import>
8 </macros>
9 <command interpreter="python">gatk_wrapper.py
10 --max_jvm_heap_fraction "1"
11 --stdout "${output_log}"
12 #for $var_count, $variant in enumerate( $reference_source.variants ):
13 -d "--input:input_${var_count},%(file_type)s" "${variant.input_variants}" "${variant.input_variants.ext}" "input_variants_${var_count}"
14 #end for
15 -p 'java
16 -jar "\$JAVA_JAR_PATH/GenomeAnalysisTK.jar"
17 -T "ApplyRecalibration"
18 ##--num_threads 4 ##hard coded, for now
19 -et "NO_ET" ##ET no phone home
20 #if $reference_source.reference_source_selector != "history":
21 -R "${reference_source.ref_file.fields.path}"
22 #end if
23 --recal_file "${reference_source.input_recal}"
24 --tranches_file "${reference_source.input_tranches}"
25 --out "${output_variants}"
26 '
27
28 #include source=$standard_gatk_options#
29
30 ##start analysis specific options
31 -p '
32 --mode "${mode}"
33
34 #for $ignore_filter in $ignore_filters:
35 #set $ignore_filter_name = str( $ignore_filter.ignore_filter_type.ignore_filter_type_selector )
36 #if $ignore_filter_name == "custom":
37 #set $ignore_filter_name = str( $ignore_filter.ignore_filter_type.filter_name )
38 #end if
39 --ignore_filter "${ignore_filter_name}"
40 #end for
41 --ts_filter_level "${ts_filter_level}"
42 '
43 </command>
44 <inputs>
45 <conditional name="reference_source">
46 <expand macro="reference_source_selector_param" />
47 <when value="cached">
48 <repeat name="variants" title="Variant" min="1" help="-input,--input &amp;lt;input&amp;gt;">
49 <param name="input_variants" type="data" format="vcf" label="Variant file to annotate"/>
50 </repeat>
51 <param name="input_recal" type="data" format="gatk_recal" label="Variant Recalibration file" help="-recalFile,--recal_file &amp;lt;recal_file&amp;gt;" />
52 <param name="input_tranches" type="data" format="gatk_tranche" label="Variant Tranches file" help="-tranchesFile,--tranches_file &amp;lt;tranches_file&amp;gt;" />
53 <param name="ref_file" type="select" label="Using reference genome" help="-R,--reference_sequence &amp;lt;reference_sequence&amp;gt;">
54 <options from_data_table="gatk_picard_indexes">
55 <!-- <filter type="data_meta" key="dbkey" ref="variants[0].input_variants" column="dbkey"/> -->
56 </options>
57 <validator type="no_options" message="A built-in reference genome is not available for the build associated with the selected input file"/>
58 </param>
59 </when>
60 <when value="history"> <!-- FIX ME!!!! -->
61 <repeat name="variants" title="Variant" min="1" help="-input,--input &amp;lt;input&amp;gt;">
62 <param name="input_variants" type="data" format="vcf" label="Variant file to annotate" />
63 </repeat>
64 <param name="input_recal" type="data" format="gatk_recal" label="Variant Recalibration file" help="-recalFile,--recal_file &amp;lt;recal_file&amp;gt;" />
65 <param name="input_tranches" type="data" format="gatk_tranche" label="Variant Tranches file" help="-tranchesFile,--tranches_file &amp;lt;tranches_file&amp;gt;" />
66 <param name="ref_file" type="data" format="fasta" label="Using reference file" help="-R,--reference_sequence &amp;lt;reference_sequence&amp;gt;" />
67 </when>
68 </conditional>
69
70 <expand macro="gatk_param_type_conditional" />
71
72 <param name="mode" type="select" label="Recalibration mode" help="-mode,--mode &amp;lt;mode&amp;gt;">
73 <option value="SNP" selected="True">SNP</option>
74 <option value="INDEL">INDEL</option>
75 <option value="BOTH">BOTH</option>
76 </param>
77 <repeat name="ignore_filters" title="Ignore Filter" help="-ignoreFilter,--ignore_filter &amp;lt;ignore_filter&amp;gt;">
78 <conditional name="ignore_filter_type">
79 <param name="ignore_filter_type_selector" type="select" label="Filter Type">
80 <option value="HARD_TO_VALIDATE">HARD_TO_VALIDATE</option>
81 <option value="LowQual" >LowQual</option>
82 <option value="custom" selected="True">Other</option>
83 </param>
84 <when value="custom">
85 <param name="filter_name" type="text" value="" label="Filter name"/>
86 </when>
87 <when value="HARD_TO_VALIDATE" />
88 <when value="LowQual" />
89 </conditional>
90 </repeat>
91 <param name="ts_filter_level" type="float" label="truth sensitivity level at which to start filtering, used here to indicate filtered variants in plots" value="99.0" help="-ts_filter_level,--ts_filter_level &amp;lt;ts_filter_level&amp;gt;"/>
92 </inputs>
93 <outputs>
94 <data format="vcf" name="output_variants" label="${tool.name} on ${on_string} (Variants File)" />
95 <data format="txt" name="output_log" label="${tool.name} on ${on_string} (log)" />
96 </outputs>
97 <tests>
98 <!-- ADD TESTS -->
99 </tests>
100 <help>
101 **What it does**
102
103 Applies cuts to the input vcf file (by adding filter lines) to achieve the desired novel FDR levels which were specified during VariantRecalibration
104
105 For more information on using the ApplyRecalibration module, see this `tool specific page &lt;http://www.broadinstitute.org/gsa/wiki/index.php/Variant_quality_score_recalibration&gt;`_.
106
107 To learn about best practices for variant detection using GATK, see this `overview &lt;http://www.broadinstitute.org/gsa/wiki/index.php/Best_Practice_Variant_Detection_with_the_GATK_v3&gt;`_.
108
109 If you encounter errors, please view the `GATK FAQ &lt;http://www.broadinstitute.org/gsa/wiki/index.php/Frequently_Asked_Questions&gt;`_.
110
111 ------
112
113 **Inputs**
114
115 GenomeAnalysisTK: ApplyRecalibration accepts a variant input file, a recalibration file and a tranches file.
116
117
118 **Outputs**
119
120 The output is in VCF format.
121
122
123 Go `here &lt;http://www.broadinstitute.org/gsa/wiki/index.php/Input_files_for_the_GATK&gt;`_ for details on GATK file formats.
124
125 -------
126
127 **Settings**::
128
129
130 recal_file The output recal file used by ApplyRecalibration
131 tranches_file The input tranches file describing where to cut the data
132 out The output filtered, recalibrated VCF file
133 ts_filter_level The truth sensitivity level at which to start filtering
134 ignore_filter If specified the optimizer will use variants even if the specified filter name is marked in the input VCF file
135 mode Recalibration mode to employ: 1.) SNP for recalibrating only SNPs (emitting indels untouched in the output VCF); 2.) INDEL for indels; and 3.) BOTH for recalibrating both SNPs and indels simultaneously. (SNP|INDEL|BOTH)
136
137 @CITATION_SECTION@
138 </help>
139 </tool>