Galaxy tool MarkDuplicates run at 12/05/2011 14:34:29
The following output files were created (click the filename to view/download a copy):
Picard on line resources
Picard output (transposed to make it easier to see)
| ## net.sf.picard.metrics.StringHeader |
| # net.sf.picard.sam.MarkDuplicates INPUT=/tmp/6729253.1.all.q/tmpM5wI_h/database/files/001/dataset_1097.dat OUTPUT=/tmp/6729253.1.all.q/tmpM5wI_h/database/files/001/dataset_1098.dat METRICS_FILE=/udd/rerla/galaxy-central/database/job_working_directory/1032/dataset_1099_files/MarkDuplicates.metrics.txt REMOVE_DUPLICATES=true ASSUME_SORTED=true READ_NAME_REGEX=[a-zA-Z0-9]+:[0-9]:([0-9]+):([0-9]+):([0-9]+).* OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 VALIDATION_STRINGENCY=LENIENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 TMP_DIR=/tmp/rerla VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false |
| ## net.sf.picard.metrics.StringHeader |
| # Started on: Thu May 12 14:34:28 EDT 2011 |
| ## METRICS CLASS net.sf.picard.sam.DuplicationMetrics |
| LIBRARY | |
| UNPAIRED_READS_EXAMINED | 1 |
| READ_PAIRS_EXAMINED | 3 |
| UNMAPPED_READS | 1 |
| UNPAIRED_READ_DUPLICATES | 1 |
| READ_PAIR_DUPLICATES | 1 |
| READ_PAIR_OPTICAL_DUPLICATES | 0 |
| PERCENT_DUPLICATION | 0.428571 |
| ESTIMATED_LIBRARY_SIZE
| 3
|
| ## HISTOGRAM java.lang.Double |
| BIN VALUE |
| 1.0 0.948181 |
| 2.0 1.296997 |
| 3.0 1.425319 |
| 4.0 1.472527 |
| 5.0 1.489893 |
| 6.0 1.496282 |
| 7.0 1.498632 |
| 8.0 1.499497 |
| 9.0 1.499815 |
| 10.0 1.499932 |
| 11.0 1.499975 |
| 12.0 1.499991 |
| 13.0 1.499997 |
| 14.0 1.499999 |
| 15.0 1.5 |
| 16.0 1.5 |
| 17.0 1.5 |
| 18.0 1.5 |
| 19.0 1.5 |
| 20.0 1.5 |
| 21.0 1.5 |
| 22.0 1.5 |
| 23.0 1.5 |
| 24.0 1.5 |
| 25.0 1.5 |
| 26.0 1.5 |
| 27.0 1.5 |
| 28.0 1.5 |
| 29.0 1.5 |
| 30.0 1.5 |
| 31.0 1.5 |
| 32.0 1.5 |
| 33.0 1.5 |
| 34.0 1.5 |
| 35.0 1.5 |
| 36.0 1.5 |
| 37.0 1.5 |
| 38.0 1.5 |
| 39.0 1.5 |
| 40.0 1.5 |
| 41.0 1.5 |
| 42.0 1.5 |
| 43.0 1.5 |
| 44.0 1.5 |
| 45.0 1.5 |
| 46.0 1.5 |
| 47.0 1.5 |
| 48.0 1.5 |
| 49.0 1.5 |
| 50.0 1.5 |
| 51.0 1.5 |
| 52.0 1.5 |
| 53.0 1.5 |
| 54.0 1.5 |
| 55.0 1.5 |
| 56.0 1.5 |
| 57.0 1.5 |
| 58.0 1.5 |
| 59.0 1.5 |
| 60.0 1.5 |
| 61.0 1.5 |
| 62.0 1.5 |
| 63.0 1.5 |
| 64.0 1.5 |
| 65.0 1.5 |
| 66.0 1.5 |
| 67.0 1.5 |
| 68.0 1.5 |
| 69.0 1.5 |
| 70.0 1.5 |
| 71.0 1.5 |
| 72.0 1.5 |
| 73.0 1.5 |
| 74.0 1.5 |
| 75.0 1.5 |
| 76.0 1.5 |
| 77.0 1.5 |
| 78.0 1.5 |
| 79.0 1.5 |
| 80.0 1.5 |
| 81.0 1.5 |
| 82.0 1.5 |
| 83.0 1.5 |
| 84.0 1.5 |
| 85.0 1.5 |
| 86.0 1.5 |
| 87.0 1.5 |
| 88.0 1.5 |
| 89.0 1.5 |
| 90.0 1.5 |
| 91.0 1.5 |
| 92.0 1.5 |
| 93.0 1.5 |
| 94.0 1.5 |
| 95.0 1.5 |
| 96.0 1.5 |
| 97.0 1.5 |
| 98.0 1.5 |
| 99.0 1.5 |
Picard Tool Run Log
Thu, 12 May 2011 14:34:29 INFO
## executing java -Xmx2g -jar /udd/rerla/galaxy-central/tool-data/shared/jars/MarkDuplicates.jar VALIDATION_STRINGENCY=LENIENT ASSUME_SORTED=true INPUT=/tmp/6729253.1.all.q/tmpM5wI_h/database/files/001/dataset_1097.dat OUTPUT=/tmp/6729253.1.all.q/tmpM5wI_h/database/files/001/dataset_1098.dat METRICS_FILE=/udd/rerla/galaxy-central/database/job_working_directory/1032/dataset_1099_files/MarkDuplicates.metrics.txt REMOVE_DUPLICATES=true READ_NAME_REGEX="[a-zA-Z0-9]+:[0-9]:([0-9]+):([0-9]+):([0-9]+).*" OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 returned status 0 and stderr:
[Thu May 12 14:34:28 EDT 2011] net.sf.picard.sam.MarkDuplicates INPUT=/tmp/6729253.1.all.q/tmpM5wI_h/database/files/001/dataset_1097.dat OUTPUT=/tmp/6729253.1.all.q/tmpM5wI_h/database/files/001/dataset_1098.dat METRICS_FILE=/udd/rerla/galaxy-central/database/job_working_directory/1032/dataset_1099_files/MarkDuplicates.metrics.txt REMOVE_DUPLICATES=true ASSUME_SORTED=true READ_NAME_REGEX=[a-zA-Z0-9]+:[0-9]:([0-9]+):([0-9]+):([0-9]+).* OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 VALIDATION_STRINGENCY=LENIENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 TMP_DIR=/tmp/rerla VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false
INFO 2011-05-12 14:34:28 MarkDuplicates Start of doWork freeMemory: 8644632; totalMemory: 9109504; maxMemory: 1908932608
INFO 2011-05-12 14:34:28 MarkDuplicates Reading input file and constructing read end information.
INFO 2011-05-12 14:34:28 MarkDuplicates Will retain up to 7575129 data points before spilling to disk.
INFO 2011-05-12 14:34:28 MarkDuplicates Read 7 records. 0 pairs never matched.
INFO 2011-05-12 14:34:29 MarkDuplicates After buildSortedReadEndLists freeMemory: 8316168; totalMemory: 130351104; maxMemory: 1908932608
INFO 2011-05-12 14:34:29 MarkDuplicates Will retain up to 59654144 duplicate indices before spilling to disk.
INFO 2011-05-12 14:34:29 MarkDuplicates Traversing read pair information and detecting duplicates.
INFO 2011-05-12 14:34:29 MarkDuplicates Traversing fragment information and detecting duplicates.
INFO 2011-05-12 14:34:29 MarkDuplicates Sorting list of duplicate records.
INFO 2011-05-12 14:34:29 MarkDuplicates After generateDuplicateIndexes freeMemory: 129615120; totalMemory: 607649792; maxMemory: 1908932608
INFO 2011-05-12 14:34:29 MarkDuplicates Marking 3 records as duplicates.
INFO 2011-05-12 14:34:29 MarkDuplicates Found 0 optical duplicate clusters.
INFO 2011-05-12 14:34:29 MarkDuplicates Before output close freeMemory: 128997520; totalMemory: 607649792; maxMemory: 1908932608
INFO 2011-05-12 14:34:29 MarkDuplicates After output close freeMemory: 128997280; totalMemory: 607649792; maxMemory: 1908932608
[Thu May 12 14:34:29 EDT 2011] net.sf.picard.sam.MarkDuplicates done.
Runtime.totalMemory()=607649792
The freely available
Picard software
generated all outputs reported here running as a
Galaxy tool