Mercurial > repos > devteam > fasta_compute_length
comparison fasta_compute_length.xml @ 2:d75972d4bd2a draft
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/fasta_compute_length commit 6e148b31fed1b322ce720804d8525088ec6d43f9
| author | devteam |
|---|---|
| date | Thu, 29 Oct 2015 22:14:42 -0400 |
| parents | 2811169ce62b |
| children | 19caae8fd9d4 |
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| 1:2811169ce62b | 2:d75972d4bd2a |
|---|---|
| 1 <tool id="fasta_compute_length" name="Compute sequence length" version="1.0.0"> | 1 <tool id="fasta_compute_length" name="Compute sequence length" version="1.0.1"> |
| 2 <description></description> | 2 <description></description> |
| 3 <command interpreter="python">fasta_compute_length.py $input $output $keep_first</command> | 3 <command interpreter="python">fasta_compute_length.py $input $output $keep_first $keep_first_word</command> |
| 4 <inputs> | 4 <inputs> |
| 5 <param name="input" type="data" format="fasta" label="Compute length for these sequences"/> | 5 <param name="input" type="data" format="fasta" label="Compute length for these sequences"/> |
| 6 <param name="keep_first" type="integer" value="0" label="How many title characters to keep?" help="'0' = keep the whole thing"/> | 6 <param name="keep_first" type="integer" value="0" label="How many title characters to keep?" help="'0' = keep the whole thing"/> |
| 7 </inputs> | 7 <param name="keep_first_word" type="boolean" truevalue="id_only" falsevalue="id_and_desc" |
| 8 <outputs> | 8 selected="false" label="Strip fasta description from header?" |
| 9 <data name="output" format="tabular"/> | 9 help="Stripping the description will truncate the fasta header to just the sequence ID. Otherwise the header description will be kept. This step is done before the 'How many characters to keep' option."/> |
| 10 </outputs> | 10 |
| 11 <tests> | 11 </inputs> |
| 12 <test> | 12 <outputs> |
| 13 <param name="input" value="454.fasta" /> | 13 <data name="output" format="tabular"/> |
| 14 <param name="keep_first" value="0"/> | 14 </outputs> |
| 15 <output name="output" file="fasta_tool_compute_length_1.out" /> | 15 <tests> |
| 16 </test> | 16 <test> |
| 17 | 17 <param name="input" value="454.fasta" /> |
| 18 <test> | 18 <param name="keep_first" value="0"/> |
| 19 <param name="input" value="extract_genomic_dna_out1.fasta" /> | 19 <param name="keep_first_word" value="id_and_desc" /> |
| 20 <param name="keep_first" value="0"/> | 20 <output name="output" file="fasta_tool_compute_length_1.out" /> |
| 21 <output name="output" file="fasta_tool_compute_length_2.out" /> | 21 </test> |
| 22 </test> | 22 |
| 23 | 23 <test> |
| 24 <test> | 24 <param name="input" value="extract_genomic_dna_out1.fasta" /> |
| 25 <param name="input" value="454.fasta" /> | 25 <param name="keep_first" value="0"/> |
| 26 <param name="keep_first" value="14"/> | 26 <param name="keep_first_word" value="id_and_desc" /> |
| 27 <output name="output" file="fasta_tool_compute_length_3.out" /> | 27 <output name="output" file="fasta_tool_compute_length_2.out" /> |
| 28 </test> | 28 </test> |
| 29 </tests> | 29 |
| 30 <help> | 30 <test> |
| 31 <param name="input" value="454.fasta" /> | |
| 32 <param name="keep_first" value="14"/> | |
| 33 <param name="keep_first_word" value="id_and_desc" /> | |
| 34 <output name="output" file="fasta_tool_compute_length_3.out" /> | |
| 35 </test> | |
| 36 </tests> | |
| 37 <help> | |
| 31 | 38 |
| 32 **What it does** | 39 **What it does** |
| 33 | 40 |
| 34 This tool counts the length of each fasta sequence in the file. The output file has two columns per line (separated by tab): fasta titles and lengths of the sequences. The option *How many characters to keep?* allows to select a specified number of letters from the beginning of each FASTA entry. | 41 This tool counts the length of each fasta sequence in the file. The output file has two columns per line (separated by tab): fasta titles and lengths of the sequences. The option *How many characters to keep?* allows to select a specified number of letters from the beginning of each FASTA entry. |
| 35 | 42 |
| 36 ----- | 43 ----- |
| 37 | 44 |
| 38 **Example** | 45 **Example** |
| 39 | 46 |
| 40 Suppose you have the following FASTA formatted sequences from a Roche (454) FLX sequencing run:: | 47 Suppose you have the following FASTA formatted sequences from a Roche (454) FLX sequencing run:: |
| 41 | 48 |
| 44 TTCGGCCGGCCCTTCTCGTCGAGGAATGACACCAGCGCTTCGCCCACG | 51 TTCGGCCGGCCCTTCTCGTCGAGGAATGACACCAGCGCTTCGCCCACG |
| 45 >EYKX4VC02D4GS2 length=60 xy=1573_3972 region=2 run=R_2007_11_07_16_15_57_ | 52 >EYKX4VC02D4GS2 length=60 xy=1573_3972 region=2 run=R_2007_11_07_16_15_57_ |
| 46 AATAAAACTAAATCAGCAAAGACTGGCAAATACTCACAGGCTTATACAATACAAATGTAAfa | 53 AATAAAACTAAATCAGCAAAGACTGGCAAATACTCACAGGCTTATACAATACAAATGTAAfa |
| 47 | 54 |
| 48 Running this tool while setting **How many characters to keep?** to **14** will produce this:: | 55 Running this tool while setting **How many characters to keep?** to **14** will produce this:: |
| 49 | 56 |
| 50 EYKX4VC02EQLO5 108 | 57 EYKX4VC02EQLO5 108 |
| 51 EYKX4VC02D4GS2 60 | 58 EYKX4VC02D4GS2 60 |
| 59 | |
| 60 However, if your IDs are not all the same length, you may wish to just keep the fasta ID, and not the description:: | |
| 61 | |
| 62 >EYKX4VC02EQLO5 length=108 xy=1826_0455 region=2 run=R_2007_11_07_16_15_57_ | |
| 63 TCCGCGCCGAGCATGCCCATCTTGGATTCCGGCGCGATGACCATCGCCCGCTCCACCACG | |
| 64 TTCGGCCGGCCCTTCTCGTCGAGGAATGACACCAGCGCTTCGCCCACG | |
| 65 >EYKX4VC length=60 xy=1573_3972 region=2 run=R_2007_11_07_16_15_57_ | |
| 66 AATAAAACTAAATCAGCAAAGACTGGCAAATACTCACAGGCTTATACAATACAAATGTAAfa | |
| 67 | |
| 68 Running this tool with **Strip fasta description from header** set to **True** and **How many characters to keep?** set to **0** will produce:: | |
| 69 | |
| 70 EYKX4VC02EQLO5 108 | |
| 71 EYKX4VC 60 | |
| 52 | 72 |
| 53 | 73 |
| 54 </help> | 74 </help> |
| 75 <citations> | |
| 76 <citation type="doi">10.1093/bioinformatics/btq281</citation> | |
| 77 </citations> | |
| 55 </tool> | 78 </tool> |
