Mercurial > repos > davidvanzessen > mutation_analysis
comparison aa_histogram.r @ 56:a4317b006d70 draft
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| author | davidvanzessen |
|---|---|
| date | Tue, 01 Mar 2016 05:06:47 -0500 |
| parents | d4e72eeea640 |
| children | cb66d6dd1e66 |
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| 55:0d5add1a9800 | 56:a4317b006d70 |
|---|---|
| 3 args <- commandArgs(trailingOnly = TRUE) | 3 args <- commandArgs(trailingOnly = TRUE) |
| 4 | 4 |
| 5 input = args[1] | 5 input = args[1] |
| 6 outfile = args[2] | 6 outfile = args[2] |
| 7 | 7 |
| 8 print("---------------- read input ----------------") | |
| 9 | |
| 8 dat = read.table(input, sep="\t", fill=T, header=T, quote="") | 10 dat = read.table(input, sep="\t", fill=T, header=T, quote="") |
| 9 | 11 |
| 10 | 12 print("---------------- as numeric ----------------") |
| 11 | 13 |
| 12 mutations.at.position = as.numeric(dat[1,]) | 14 mutations.at.position = as.numeric(dat[1,]) |
| 13 aa.at.position = as.numeric(dat[2,]) | 15 aa.at.position = as.numeric(dat[2,]) |
| 14 | 16 |
| 17 print("---------------- freq data.frame ----------------") | |
| 18 | |
| 15 dat_freq = mutations.at.position / aa.at.position | 19 dat_freq = mutations.at.position / aa.at.position |
| 16 dat_dt = data.frame(i=1:length(dat_freq), freq=dat_freq) | 20 dat_dt = data.frame(i=1:length(dat_freq), freq=dat_freq) |
| 21 | |
| 22 print("---------------- weird stuff ----------------") | |
| 17 | 23 |
| 18 options(width=220) | 24 options(width=220) |
| 19 | 25 |
| 20 print(dat[,20:40]) | 26 print(dat[,20:40]) |
| 21 | 27 |
| 22 print(dat_dt) #need this or it will fail???? | 28 print(dat_dt) #need this or it will fail???? |
| 29 | |
| 30 print("---------------- plot ----------------") | |
| 23 | 31 |
| 24 m = ggplot(dat_dt, aes(x=i, y=freq)) + theme(axis.text.x = element_text(angle = 90, hjust = 1)) | 32 m = ggplot(dat_dt, aes(x=i, y=freq)) + theme(axis.text.x = element_text(angle = 90, hjust = 1)) |
| 25 m = m + geom_histogram(stat="identity", colour = "black", fill = "darkgrey", alpha=0.8) + scale_x_continuous(breaks=1:length(dat_freq), labels=1:length(dat_freq)) | 33 m = m + geom_histogram(stat="identity", colour = "black", fill = "darkgrey", alpha=0.8) + scale_x_continuous(breaks=1:length(dat_freq), labels=1:length(dat_freq)) |
| 26 m = m + annotate("segment", x = 0.5, y = -0.05, xend=26.5, yend=-0.05, colour="darkgreen", size=1) + annotate("text", x = 13, y = -0.1, label="FR1") | 34 m = m + annotate("segment", x = 0.5, y = -0.05, xend=26.5, yend=-0.05, colour="darkgreen", size=1) + annotate("text", x = 13, y = -0.1, label="FR1") |
| 27 m = m + annotate("segment", x = 26.5, y = -0.07, xend=38.5, yend=-0.07, colour="darkblue", size=1) + annotate("text", x = 32.5, y = -0.15, label="CDR1") | 35 m = m + annotate("segment", x = 26.5, y = -0.07, xend=38.5, yend=-0.07, colour="darkblue", size=1) + annotate("text", x = 32.5, y = -0.15, label="CDR1") |
