Mercurial > repos > davidvanzessen > mutation_analysis
comparison wrapper.sh @ 99:86206431cbb0 draft
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author | davidvanzessen |
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date | Thu, 16 Jun 2016 10:01:54 -0400 |
parents | 5ffbf40cdd4b |
children | ff5be711382b |
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98:5ffbf40cdd4b | 99:86206431cbb0 |
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91 cat `find $PWD/files/ -name "7_*"` > "$outdir/new_IMGT/7_V-REGION-mutation-and-AA-change-table.txt" | 91 cat `find $PWD/files/ -name "7_*"` > "$outdir/new_IMGT/7_V-REGION-mutation-and-AA-change-table.txt" |
92 cat `find $PWD/files/ -name "8_*"` > "$outdir/new_IMGT/8_V-REGION-nt-mutation-statistics.txt" | 92 cat `find $PWD/files/ -name "8_*"` > "$outdir/new_IMGT/8_V-REGION-nt-mutation-statistics.txt" |
93 cat `find $PWD/files/ -name "9_*"` > "$outdir/new_IMGT/9_V-REGION-AA-change-statistics.txt" | 93 cat `find $PWD/files/ -name "9_*"` > "$outdir/new_IMGT/9_V-REGION-AA-change-statistics.txt" |
94 cat `find $PWD/files/ -name "10_*"` > "$outdir/new_IMGT/10_V-REGION-mutation-hotspots.txt" | 94 cat `find $PWD/files/ -name "10_*"` > "$outdir/new_IMGT/10_V-REGION-mutation-hotspots.txt" |
95 | 95 |
96 Rscript $dir/tmp/igat.r $outdir/new_IMGT/ $outdir/merged.txt 2>&1 | 96 mkdir $outdir/new_IMGT_ca |
97 cp $outdir/new_IMGT/* $outdir/new_IMGT_ca | |
98 | |
99 mkdir $outdir/new_IMGT_cg | |
100 cp $outdir/new_IMGT/* $outdir/new_IMGT_cg | |
101 | |
102 mkdir $outdir/new_IMGT_cm | |
103 cp $outdir/new_IMGT/* $outdir/new_IMGT_cm | |
104 | |
105 Rscript $dir/tmp/igat.r $outdir/new_IMGT/ $outdir/merged.txt "-" 2>&1 | |
106 Rscript $dir/tmp/igat.r $outdir/new_IMGT_ca/ $outdir/merged.txt "ca "2>&1 | |
107 Rscript $dir/tmp/igat.r $outdir/new_IMGT_cg/ $outdir/merged.txt "cg "2>&1 | |
108 Rscript $dir/tmp/igat.r $outdir/new_IMGT_cm/ $outdir/merged.txt "cm "2>&1 | |
97 | 109 |
98 | 110 |
99 tmp="$PWD" | 111 tmp="$PWD" |
100 cd $outdir/new_IMGT/ #tar weirdness... | 112 cd $outdir/new_IMGT/ #tar weirdness... |
101 tar -cJf ../new_IMGT.txz * | 113 tar -cJf ../new_IMGT.txz * |
102 | |
103 cp $dir/tmp/IgAT.xlsm $outdir/new_IMGT/IgAT.xlsm | 114 cp $dir/tmp/IgAT.xlsm $outdir/new_IMGT/IgAT.xlsm |
104 | |
105 #tar -cJf ../IgAT.txz * | |
106 zip -r ../IgAT.zip * | 115 zip -r ../IgAT.zip * |
107 | 116 |
117 cd $outdir/new_IMGT_ca/ | |
118 tar -cJf ../new_IMGT_ca.txz * | |
119 cp $dir/tmp/IgAT.xlsm $outdir/new_IMGT_ca/IgAT.xlsm | |
120 zip -r ../IgAT_ca.zip * | |
121 | |
122 cd $outdir/new_IMGT_cg/ | |
123 tar -cJf ../new_IMGT_cg.txz * | |
124 cp $dir/tmp/IgAT.xlsm $outdir/new_IMGT_cg/IgAT.xlsm | |
125 zip -r ../IgAT_cg.zip * | |
126 | |
127 cd $outdir/new_IMGT_cm/ | |
128 tar -cJf ../new_IMGT_cm.txz * | |
129 cp $dir/tmp/IgAT.xlsm $outdir/new_IMGT_cm/IgAT.xlsm | |
130 zip -r ../IgAT_cm.zip * | |
131 | |
108 cd $tmp | 132 cd $tmp |
109 | |
110 | 133 |
111 echo "---------------- mutation_analysis.r ----------------" | 134 echo "---------------- mutation_analysis.r ----------------" |
112 echo "---------------- mutation_analysis.r ----------------<br />" >> $output | 135 echo "---------------- mutation_analysis.r ----------------<br />" >> $output |
113 | 136 |
114 classes="ca,ca1,ca2,cg,cg1,cg2,cg3,cg4,cm,unmatched" | 137 classes="ca,ca1,ca2,cg,cg1,cg2,cg3,cg4,cm,unmatched" |
134 echo "---------------- sequence_overview.r ----------------" >> $output | 157 echo "---------------- sequence_overview.r ----------------" >> $output |
135 | 158 |
136 mkdir $outdir/sequence_overview | 159 mkdir $outdir/sequence_overview |
137 | 160 |
138 #Rscript $dir/sequence_overview.r $outdir/identified_genes.txt $PWD/sequences.txt $outdir/merged.txt $outdir/sequence_overview $classes $outdir/hotspot_analysis_sum.txt 2>&1 | 161 #Rscript $dir/sequence_overview.r $outdir/identified_genes.txt $PWD/sequences.txt $outdir/merged.txt $outdir/sequence_overview $classes $outdir/hotspot_analysis_sum.txt 2>&1 |
139 Rscript $dir/sequence_overview.r $outdir/before_unique_filter.txt $outdir/merged.txt $outdir/sequence_overview $classes $outdir/hotspot_analysis_sum.txt 2>&1 | 162 Rscript $dir/sequence_overview.r $outdir/before_unique_filter.txt $outdir/sequence_overview $classes $outdir/hotspot_analysis_sum.txt 2>&1 |
140 | 163 |
141 echo "<table border='1'>" > $outdir/base_overview.html | 164 echo "<table border='1'>" > $outdir/base_overview.html |
142 | 165 |
143 while IFS=$'\t' read ID class seq A C G T | 166 while IFS=$'\t' read ID class seq A C G T |
144 do | 167 do |
205 echo "<a href='absent_aa_id.txt'>Absant AA locations by id</a><br />" >> $output | 228 echo "<a href='absent_aa_id.txt'>Absant AA locations by id</a><br />" >> $output |
206 echo "<a href='sequence_overview/index.html'>Sequence Overview</a><br />" >> $output | 229 echo "<a href='sequence_overview/index.html'>Sequence Overview</a><br />" >> $output |
207 echo "<a href='base_overview.html'>Base overview</a><br />" >> $output | 230 echo "<a href='base_overview.html'>Base overview</a><br />" >> $output |
208 echo "<a href='baseline.pdf'>Baseline PDF</a><br />" >> $output | 231 echo "<a href='baseline.pdf'>Baseline PDF</a><br />" >> $output |
209 echo "<a href='baseline.txt'>Baseline Table</a><br />" >> $output | 232 echo "<a href='baseline.txt'>Baseline Table</a><br />" >> $output |
233 echo "<a href='baseline_ca.pdf'>Baseline ca PDF</a><br />" >> $output | |
234 echo "<a href='baseline_ca.txt'>Baseline ca Table</a><br />" >> $output | |
235 echo "<a href='baseline_cg.pdf'>Baseline cg PDF</a><br />" >> $output | |
236 echo "<a href='baseline_cg.txt'>Baseline cg Table</a><br />" >> $output | |
237 echo "<a href='baseline_cm.pdf'>Baseline cm PDF</a><br />" >> $output | |
238 echo "<a href='baseline_cm.txt'>Baseline cm Table</a><br />" >> $output | |
210 echo "<a href='IgAT.zip'>IgAT zip</a><br />" >> $output | 239 echo "<a href='IgAT.zip'>IgAT zip</a><br />" >> $output |
240 echo "<a href='IgAT_ca.zip'>IgAT ca zip</a><br />" >> $output | |
241 echo "<a href='IgAT_cg.zip'>IgAT cg zip</a><br />" >> $output | |
242 echo "<a href='IgAT_cm.zip'>IgAT cm zip</a><br />" >> $output | |
243 echo "<a href='new_IMGT.txz'>Filtered IMGT zip</a><br />" >> $output | |
244 echo "<a href='new_IMGT_ca.txz'>Filtered ca IMGT zip</a><br />" >> $output | |
245 echo "<a href='new_IMGT_cg.txz'>Filtered cg IMGT zip</a><br />" >> $output | |
246 echo "<a href='new_IMGT_cm.txz'>Filtered cm IMGT zip</a><br />" >> $output | |
247 | |
211 | 248 |
212 echo "---------------- images ----------------" | 249 echo "---------------- images ----------------" |
213 | 250 |
214 echo "<img src='all.png'/><br />" >> $output | 251 echo "<img src='all.png'/><br />" >> $output |
215 echo "<a href='all.txt'>download data</a><br />" >> $output | 252 echo "<a href='all.txt'>download data</a><br />" >> $output |
258 echo "</table>" >> $output | 295 echo "</table>" >> $output |
259 | 296 |
260 echo "</html>" >> $output | 297 echo "</html>" >> $output |
261 | 298 |
262 echo "---------------- baseline ----------------" | 299 echo "---------------- baseline ----------------" |
263 bash $dir/tmp/baseline/wrapper.sh 1 1 1 1 0 0 "25:26:38:55:65:104:-" $outdir/new_IMGT.txz "sample name" "$dir/tmp/baseline/IMGT-reference-seqs-IGHV-2015-11-05.fa" "$outdir/baseline.pdf" "Sequence.ID" "$outdir/baseline.txt" | 300 bash $dir/tmp/baseline/wrapper.sh 1 1 1 1 0 0 "25:26:38:55:65:104:-" $outdir/new_IMGT.txz "ca_cg_cm" "$dir/tmp/baseline/IMGT-reference-seqs-IGHV-2015-11-05.fa" "$outdir/baseline.pdf" "Sequence.ID" "$outdir/baseline.txt" |
301 bash $dir/tmp/baseline/wrapper.sh 1 1 1 1 0 0 "25:26:38:55:65:104:-" $outdir/new_IMGT_ca.txz "ca" "$dir/tmp/baseline/IMGT-reference-seqs-IGHV-2015-11-05.fa" "$outdir/baseline_ca.pdf" "Sequence.ID" "$outdir/baseline_ca.txt" | |
302 bash $dir/tmp/baseline/wrapper.sh 1 1 1 1 0 0 "25:26:38:55:65:104:-" $outdir/new_IMGT_cg.txz "cg" "$dir/tmp/baseline/IMGT-reference-seqs-IGHV-2015-11-05.fa" "$outdir/baseline_cg.pdf" "Sequence.ID" "$outdir/baseline_cg.txt" | |
303 bash $dir/tmp/baseline/wrapper.sh 1 1 1 1 0 0 "25:26:38:55:65:104:-" $outdir/new_IMGT_cm.txz "cm" "$dir/tmp/baseline/IMGT-reference-seqs-IGHV-2015-11-05.fa" "$outdir/baseline_cm.pdf" "Sequence.ID" "$outdir/baseline_cm.txt" | |
264 | 304 |
265 #optional output for naive | 305 #optional output for naive |
266 | 306 |
267 echo "---------------- naive_output.r ----------------" | 307 echo "---------------- naive_output.r ----------------" |
268 | 308 |
278 | 318 |
279 echo "</table>" >> $outdir/base_overview.html | 319 echo "</table>" >> $outdir/base_overview.html |
280 | 320 |
281 echo "---------------- Done! ----------------" | 321 echo "---------------- Done! ----------------" |
282 | 322 |
283 | |
284 | |
285 | |
286 | |
287 | |
288 | |
289 #rm $outdir/HS12RSS.txt | |
290 #rm $outdir/HS23RSS.txt |