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26
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1 library(ggplot2)
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2
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3 args <- commandArgs(trailingOnly = TRUE)
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4
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5 input = args[1]
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6 outfile = args[2]
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7
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56
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8 print("---------------- read input ----------------")
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9
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49
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10 dat = read.table(input, sep="\t", fill=T, header=T, quote="")
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11
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56
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12 print("---------------- as numeric ----------------")
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49
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13
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14 mutations.at.position = as.numeric(dat[1,])
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15 aa.at.position = as.numeric(dat[2,])
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26
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16
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56
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17 print("---------------- freq data.frame ----------------")
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18
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49
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19 dat_freq = mutations.at.position / aa.at.position
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26
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20 dat_dt = data.frame(i=1:length(dat_freq), freq=dat_freq)
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21
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56
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22 print("---------------- weird stuff ----------------")
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23
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49
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24 options(width=220)
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25
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26 print(dat[,20:40])
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27
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51
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28 print(dat_dt) #need this or it will fail????
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29
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56
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30 print("---------------- plot ----------------")
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31
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67
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32 #also need these two, I don't even know...
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33 print(paste("dat_freq", length(dat_freq)))
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34 print(paste("dat_dt", nrow(dat_dt)))
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35
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26
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36 m = ggplot(dat_dt, aes(x=i, y=freq)) + theme(axis.text.x = element_text(angle = 90, hjust = 1))
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82
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37 m = m + geom_bar(stat="identity", colour = "black", fill = "darkgrey", alpha=0.8) + scale_x_continuous(breaks=1:length(dat_freq), labels=1:length(dat_freq))
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49
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38 m = m + annotate("segment", x = 0.5, y = -0.05, xend=26.5, yend=-0.05, colour="darkgreen", size=1) + annotate("text", x = 13, y = -0.1, label="FR1")
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39 m = m + annotate("segment", x = 26.5, y = -0.07, xend=38.5, yend=-0.07, colour="darkblue", size=1) + annotate("text", x = 32.5, y = -0.15, label="CDR1")
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40 m = m + annotate("segment", x = 38.5, y = -0.05, xend=55.5, yend=-0.05, colour="darkgreen", size=1) + annotate("text", x = 47, y = -0.1, label="FR2")
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41 m = m + annotate("segment", x = 55.5, y = -0.07, xend=65.5, yend=-0.07, colour="darkblue", size=1) + annotate("text", x = 60.5, y = -0.15, label="CDR2")
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42 m = m + annotate("segment", x = 65.5, y = -0.05, xend=104.5, yend=-0.05, colour="darkgreen", size=1) + annotate("text", x = 85, y = -0.1, label="FR3")
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43 m = m + expand_limits(y=c(-0.1,1)) + xlab("AA position") + ylab("Frequency") + ggtitle("AA mutation frequency")
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57
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44
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45 print("---------------- write/print ----------------")
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46
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26
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47 write.table(dat_dt, paste(dirname(outfile), "/aa_histogram.txt", sep=""), sep="\t",quote=F,row.names=F,col.names=T)
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48
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49 png(filename=outfile, width=1280, height=720)
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50 print(m)
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51 dev.off()
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